*Sujoy Dutta** *Address *: **** Green Bay Rd, Apt 202,

North Chicago, IL60064.

Address 2: 2855 Unicornio St, Apt A, Carlsbad, CA, 92009.

Email: ab04gr@r.postjobfree.com. Tel. 224-***-****.

*Research Assistant Professor*

*Rosalind Franklin University of Medicine and Science*

*North Chicago, IL, USA*

* *

Research scientist with >6 years of extensive research experience in wide

areas of cell biology, molecular biology and immunology. An adaptable,

productive, and highly motivated researcher with experience in conceiving,

implementation and supervision of challenging research projects in diverse

areas of infectious diseases and cancer biology. Experienced in laboratory

and grant management and group leadership skills. Authored, co-authored and

published 12+ peer-reviewed manuscripts in Journals of high impact. 12+

Oral and poster presentations. Mentored postdoctoral fellows, graduate,

research technicians, undergraduate, and high school students to achieve

their research goals.

Recognized among supervisors and peers as a highly productive and

dependable scientist, who can work efficiently both as an individual

contributor as well as spearhead difficult projects as a team player. Known

to be a champion of using new methodology, assay development and efficient

project planning to minimize time and maximize research output. *Research

expertise:*

*Projects in BSL level 2 and 1*

*Cell culture methods (including 3D culture)*

*In vivo** infectious disease models (mice)*

*Vaccine development*

*Signal pathway analysis*

*DNA and RNA analysis*

*Molecular Cloning*

*Lenti-viral systems*

*Gene expression studies*

*Flow cytometry*

*Microscopy (live cell/immuno fluorescence-multi-color)*

*Protein purifications and analysis (including HPLC and FPLC)*

*ELISA*

*CAREER MILESTONES*

Leading role in setting up of new breast cancer research

laboratory in the H.M. Bligh cancer research center at the Rosalind

Franklin University of Medicine and Science (RFUMS) and successfully

developed, procured funding and implemented 2 research projects to study

the role of breast cancer cell secreted factors (proteins and exosomes) in

tumor development and metastasis.

Played a leading role to decipher how lymphoma secreted exosomes

help cancer cells to evade innate immune surveillance.

Developed a novel intracellular microbe based universal vaccine

delivery platform.

Pioneered the use of photodynamic therapy (using phthalocyanine

photosensitizers) for treatment of global parasitic disease,

Leishmaniasis.

* *

*SKILLS*

1. *Instrumentation*: Gel Electrophoresis, Ultra-centrifugation, Bio-rad

electroporation system Gene pulser, Thermocyclers (regular and real time

applied biosystems 7500 model), Flow-cytometry and FACS (BD systems LSRII,

Aria, Gallios and Caliber), Microscopy (Nikon 80i and TE-2000), TLC,

spectro-flourimetry (LS 50B), Odyssey system (Licor), BIORAD platform for

2D protein electrophoresis, GE AKATA chromatography systems, Alphaimager

and Plate reader (BioTek).

2. *Methods*:

Cell culture: Mammalian and microbial cell line development. Culture and

maintenance of Parasitic protozoa *Leishmania* (many different species),

murine and human macrophage cell lines, murine dendritic cell lines, T cell

lines, hybridoma, differentiation and culture of murine bone marrow derived

macrophages and dendritic cells, human primary endothelial cells,

epithelial cells and fibroblasts, several cancer cell lines (breast cancer

lines: T47D, MDA 231, MCF7, SUM149PT, MO2; melanoma cell line Malme-3M),

isolation/purification and short term cultures of human and mouse

neutrophils, monocytes and lymphocytes from peripheral blood and organs

e.g. spleen. 3D cell culture.

Animal handling: mice handling (parasitic infection models, immunization

and vaccination studies), dissection and drug administration to mice. IACUC

certification for animal handling.

RNA, miRNA and DNA isolations, western blotting, southern blotting, phase

and immunofluorescence microscopy (both qualitative and quantitative PLA),

molecular cloning, PCR and real time q-PCR, DNA sequencing, protein

purification (biotin-affinity columns), immunoprecipitations, lipid-raft

isolations, enzyme assays, PCR arrays, protein arrays, flowcytometry (BD

Caliber, LSRII, Aria, Gallios; Software: FACS DIVA and FlowJo), ELISA,

si-RNA, in vitro-antigen presentation assays, thin layer chromatography,

2D-gel electrophoresis, FPLC, antibody development and LC-Mass

spectrometry. Secreted nanovesicle (exosomes) isolation.

3. *Practices*: Conceive new projects and design of experiments and

regulatory requirements (biosafety, environmental safety and ethics).

Writing of SOPs, LOIs, IRBs, Grants and manuscripts. Grant and resource

management. New laboratory set up and research group management.

Supervision of research assistants and fellows. Working in BSL level 2 and

IACUC certification for animal handling.

4. *Communication*: Communicated several manuscripts for publications as a

corresponding author. Oral and poster presentations at various conferences

and symposia. Reviewed various manuscripts for several journals as a

referee.

5. *Software*: Windows (XP, Vista, Seven), Microsoft Office (Word, Excel

and PowerPoint), Adobe Photoshop, Molecular biology and DNA sequence

analysis software: Gene Doc, Chromas, Bioedit; Flow cytometry softwares:

FACS DIVA and FlowJo Microscopy software: Metamorphosis, DuoLINK for

quantitative microscopy and co-localization analysis.

*CAREER HISTORY*

*Research Assistant Professor*

*Department of Microbiology and Immunology, Rosalind Franklin University of

Medicine and Science/Chicago Medical School. *North Chicago, IL, USA.

July 2012 to present

Setting up of new breast cancer research laboratory in the H.M.

Bligh cancer research center at the Rosalind Franklin University of

Medicine and Science (RFUMS).

Designed and developed new research projects to study the role of

cancer cell secreted factors (proteins and exosomes) in tumor development

and metastasis.

Performed, trained and supervised personnel in day-to-day bench

work.

Assay development role in research group.

Success in obtaining competitive research grant.

Established successful new collaborations within and outside of

RFUMS for ongoing projects.

Organized, directed and also participated in successful team

research projects.

Writing and communication of manuscripts.

Managed core facilities.

* *

*Senior Research Associate*

*H.M. Bligh Cancer Research Laboratory, Rosalind Franklin University of

Medicine and Science/Chicago Medical School. (Lab. of Prof. Bala

Chandran).*North Chicago, IL, USA.

February 2012 to June 2012-

- Development and implementation of research projects on breast cancer

and lymphoma

- Assay development role and team research activities.

- Technical leadership role.

*Senior Research Associate*

*Dept. of Microbiology and Immunology, Chicago Medical School, Rosalind

Franklin University of Medicine and Science.* (*Lab. of Prof. K.P. Chang*).

North Chicago, Illinois, USA.

November 2010 to January 2012

• Development and implementation of research projects in BSL level

2 settings (*in vitro* and *in vivo* disease models using small animal

models).

- Assay development and technical leadership role.

- Writing of grant applications and manuscripts.

- Training students and fellows.

- Established new research collaborations outside of RFUMS *(both in US

and international collaborations and leading successful team research

activities*).

*Post-Doctoral Research Associate *

*Chicago Medical School, Rosalind Franklin University of Medicine and

Science. *(*Lab. of Prof. K.P. Chang *North Chicago, Illinois,

USA.

November 2007 to October 2010.

- Designed and performed *in vitro and in vivo *disease model* *(mice

model) experiments in BSL level 2 settings.

- Assay development role.

- Writing and communication of manuscripts.

*EDUCATION/OTHER PROFESSIONAL SERVICE/ AFFILIATIONS/AWARDS*

Ø *AWARDS *

Principal investigator in ALGH-CMS translational pilot project award

(2012), *Title: “Role of Angiogenin in breast cancer metastasis”*.

Ø *REVIEWER OF JOURNAL *

Served as reviewer in 8 different scientific journals.

Ø *EDITORIAL BOARD MEMBER** *

Biochemistry and pharmacology: Open Access. Omics group.

Ø *EDUCATION*

*Ph.D *Ph.D. in Microbiology and Immunology, 2002 - 2007

Dept. of Microbiology and Immunology, Chicago

Medical School, Rosalind Franklin University of Medicine and Science, North

Chicago, USA.

*Thesis title:* “Photosensitization of *Leishmania* and its applications”

*M.Sc *M.Sc in Biochemistry, 1999 – 2001. University of

Calcutta, West Bengal, India.

*B.Sc* B.Sc in Chemistry (with Honors), 1996 – 1999.

University of Calcutta, West Bengal, India.* *

* *

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