Assistant Professor of Biochemistry, Protein Biochemist/Enzymologist

Dr. Kholis Abdurachim

Department of Biochemistry, College of Medicine, University of Hail, Hail, Saudi Arabia

E-mail: *******@*****.***; *.*****@***.***.**

http://faculty.uoh.edu.sa/k.audah

Mobile Phone: +966-*-***-***-**

Objective

To work as a lecturer and researcher in a research-oriented university that seeks a dedicated and good work ethic person with initiative to establish a reputable teaching and research institution.

Current Position

Sept 2009- Assistant Professor of Biochemistry

Teaching responsibility: Clinical Biochemistry, Medicinal Chemistry, and Medical Foundation courses

Administrative Experiences

Sept 2009- present Coordinator of Biochemistry Courses and Laboratory

Sept 2009-present Head of Biochemistry Laboratory

May 2010-Oct 2010 Acting Head of Department of Clinical Nutrition

Education and Research Experiences

2008-2009 Postdoctoral Research Associate (Infectious Diseases and Microbial Pathogenesis), Yale University School of Medicine, USA

2003-2007 Ph.D (Biochemistry), Auburn University, USA

1998-2000 MS (Molecular Biology)(graduated with Distinction), University of Malaya, Malaysia.

1991-1996 BS (Chemistry and Biochemistry),Bogor Agricultural University, Indonesia.

1995-1996 Biotechnology Research Center for Estate Crops, Bogor, Indonesia

June-August 1994 Research and Development Center for Applied Chemistry, Indonesian Institute of Science, Indonesia

Research Grant Achievement

Major contributor to the success of four-year research funding extension (2009-2013) from the National Institute of Health (NIH), USA to Barbara Kazmierczak, MD, PhD (Associate Professor of Internal Medicine and Microbial Pathogen) at Yale University School of Medicine

Research Interests

Proteomics Research, Protein-Protein Interactions, DNA-Protein Interactions, G-protein, Enzyme Kinetics (Steady State and Pre-Steady State), X-Ray Crystallography, Medical-Related Research (Drug Discovery, and Microbial Pathogen)

Technical Expertise

DNA manipulation (cloning, PCR, mutagenesis, large scale plasmid purification by ultracentrifugation), gene transformation, bacterial cells culture, DNA and protein electrophoresis, protein research (expression, purification, and characterization), pull-down experiments, western blotting, chemical cross-linking, chromatography, FPLC, HPLC, UV-VIS spectrophotometry, fluorescence spectroscopy, circular dichroism spectroscopy, enzyme kinetics, stopped-flow spectroscopy, MALDI-MS, and radio isotope handling.

Peer Reviewed Journal Publication

Kholis Abdurachim and Holly R. Ellis (2006). Detection of Protein-Protein Interactions in the Alkanesulfonate Monooxygenase from E. coli. The Journal of Bacteriology, vol. 188 (23): 8153-8159.

Manuscripts in Preparation

1. Kholis Abdurachim and Holly R. Ellis. Protein-Protein Interaction is Essential for Efficient Reduced Flavin Transfer in the Alkanesulfonate Monooxygenase System (ready for submission).

2.Kholis Abdurachim and Barbara Kazmierczak. Characterization of FlhF, a novel flagellar GTPase from Pseudomonas aeruginosa.

Thesis and Dissertation

1. Kholis Abdurachim (2007). Studies to Elucidate the Mechanism of Reduced Flavin Transfer in the Alkanesulfonate Monooxygenase System from Escherichia coli. Doctor of Philosophy Dissertation. Auburn University, Auburn, Alabama, United States of America.

2. Kholis Abdurachim (2000). Construction of Arginine Repressor Fusion Protein and Analysis of Its Function in Xer Site-Specific Recombination in Escherichia coli. Master of Science Thesis. University of Malaya, Malaysia.

3. Kholis Abdurachim (1996). Identification of Heat-Shock Protein from Robusta Coffee Tree as a Response to Water Stress. Bachelor of Science Thesis, Bogor Agricultural University, Indonesia.

Notable Oral Presentations

1. FlhF, a novel flagellar GTPase from Pseudomonas aeruginosa (2009). Department of Internal Medicine, Section of Infectious Diseases, Yale University School of Medicine, USA.

2. Characterization of FlhF protein from Pseudomonas aeruginosa (2009). Department of Microbial Pathogenesis, Yale University School of Medicine, USA.

Presentations and Proceedings

1. Kholis Abdurachim, Thomas S. Murray, and Barbara Kazmierczak (2009) Title: The role of FlhF in the regulation of flagella and pili assembly in Pseudomonas aeruginosa. Microbial Pathogenesis and Host Response Conference, Cold Spring Harbor Laboratory, New York.

2. Kholis Abdurachim and Barbara Kazmierczak (2008) Title: FlhF, a Novel Magnesium-Independent GTPase from Pseudomonas aeruginosa. Poster presented at the 10th Microbiology Retreat of Yale University School of Medicine, Westbrook, Connecticut.

3. Kholis Abdurachim and Holly R. Ellis (2008) Title: Physical and Kinetic Evidence for Direct Reduced Flavin Transfer in the Alkanesulfonate Monooxygenase System. Poster presented at the 108th General Meeting of the American Society for Microbiology, Boston, Massachusetts.

4. Kholis Abdurachim and Holly R. Ellis (2007) Title: The effect of Protein-Protein Interactions on the Mechanism of FMN reduction in the Alkanesulfonate Monooxygenase System. Poster presented at the 21 Symposium of Protein Science, Boston, Massachusetts.

5. Xuanzhi Zhan, Kholis Abdurachim, Russell A. Carpenter, Holly R. Ellis (2007) Title: Kinetic Investigation into the Catalytic Properties of the Alkanesulfonate Monooxygenase Enzyme. Poster presented at the Gordon Research Conference-Enzyme, Coenzymes and Metabolic Pathways Conference, Biddeford, Maine.

6. Kholis Abdurachim and Holly R. Ellis (2006) Title: Protein-Protein Interactions in the Alkanesulfonate Monooxygenase System from E. coli. Poster presented at the Trends in Enzymology Conference, Como, Italy.

7. Tsunghsueh Wu, Kholis Abdurachim, Holly R. Ellis, and Curtis Shannon (2006) Title: Characterization of Protein-Protein Interaction Using SPR and AFM. Presented at 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Orlando, Florida.

8. Kholis Abdurachim and Holly R. Ellis (2005) Title: Physical Evidence for Protein Interactions between the FMN Reductase and the Monooxygenase Enzyme in the Alkanesulfonate Monooxygenase System. Poster presented at the 19th Enzyme Mechanism Conference in Pacific Grove, California.

9. Benlian Gao, Kholis Abdurachim and Holly R. Ellis (2004) Title: Physical and Kinetic Evidence of Protein-protein Interactions in the Alkanesulfonate Monooxygenase from E. coli. Poster presented at the Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways. New Hampshire.

10. Kholis Abdurachim and Amir Feisal Merican (1999) Title: Construction of Arginine Repressor Fusion Protein and Analysis of Its Function in Xer Site-Specific Recombination in Escherichia coli. Proceeding of the 11th National Biotechnology Seminar pp299-302, Melaka, Malaysia.

11. Kholis Abdurachim and Amir Feisal Merican (1999) Title: Construction of Arginine Repressor Biotynilated Peptide Fusion Protein in Escherichia coli. Proceeding of the Graduate Student Conference of University of Malaya, Malaysia.

Teaching Experiences

Graduate Teaching Assistant (Auburn University)

Spring 2003-2007, General Chemistry Lab

Spring 2005, Organic Chemistry I Lab

Undergraduate Teaching Assistant (Bogor Agricultural University)

1993-1995, General Chemistry Lab

1994-1995, Quantitative Analysis Chemistry Lab, and Biochemistry Lab

Work Experience

January-December 2002, Center for Radiation Safety Assessment, Nuclear Energy Control Board of Indonesia.

Workshops

Phage display technology

Protein and DNA sequencing

Awards

1. American Society for Microbiology Travel Award, Boston, MA, USA (2008).

2. International Society of Quantum Biology and Pharmacology (ISQBP) Travel Award, Como, Italy (2006).

3. Nichols Fellowship, College of Science and Mathematics, Auburn University (2005).

4. College of Science and Mathematics, Auburn University Travel Award, Pacific Groove, California, USA (2005).

5. Graduate School, Auburn University Travel Award, Pacific Groove, California, USA (2005).

6. Fourth place in the poster competition (2005), Chemistry Graduate Student Association, Auburn University.

Professional Associations

1. American Society for Microbiology

2. Protein Society

3. New York Academy of Sciences

4. Yale Biotechnology and Pharmaceutical Society

Honorary Society

Phi Lambda Upsilon, National Chemical and Chemical Engineering Honorary, USA.

Volunteer Work

Spring 2007, Regional Science Olympiad, Auburn University

Spring 2006, Regional Science Olympiad, Auburn University

Spring 2003, Regional Science Olympiad, Auburn University

1999-2000, Executive member, Indonesian Student Organization in Malaysia

1999-2000, Executive member, Postgraduate Student Organization of University of Malaya

1998-1999, Executive member, Indonesian Student Organization in Malaysia (University of Malaya Branch)

References

1. Dr. Holly R. Ellis (Associate Professor of Biochemistry, Department of Chemistry and Biochemistry, Auburn University)

PhD Advisor (committee chair)

e-mail: *******@******.***

2. Dr. Evert C. Duin (Associate Professor of Biochemistry, Department of Chemistry and Biochemistry, Auburn University, USA)

PhD Advisor (committee member)

e-mail: *******@******.***

3. Dr. Barbara Kazmierczak (Associate Professor of Medicine and Microbiology, Yale University School of Medicine )

Postdoctoral Advisor

e-mail: *******.***********@****.***

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