Downstream Purification Scientist

Downstream Purification Scientist needs 2 years of hands-on purification experience in an academic or industry setting

Downstream Purification Scientist requires:

• Hands-on experience in Cesium chloride (CsCl) density gradient ultracentrifugation, ion-exchange chromatography, affinity purification, and tangential flow filtration (TFF), with a strong understanding of purification principles and data analysis.

• Master’s degree in biology, biochemistry, chemical engineering, biotechnology, or related field.

• AAV or other viral vector CsCl density gradient and column purification.

• Experience with AKTA systems and Unicorn software.

Downstream Purification Scientist duties:

• Execute purification processes including CsCl density gradient ultracentrifugation, affinity chromatography (e.g., AVB, CaptureSelect), ion-exchange chromatography (AEX, CEX), and other polishing steps. •

• Operate AKTA systems (Avant, Pure, or Pilot) for small- to large-scale purification runs. • Perform TFF operations for buffer exchange and concentration of intermediate or final products.

• Support experimental design, data collection, and documentation following established procedures and GMP/GLP best practices.

• Assist in troubleshooting and optimization of purification processes.

• Analyze in-process and purified samples using relevant analytical methods (e.g., UV, SDS-PAGE, ddPCR, ELISA, or HPLC).

• Collaborate cross-functionally with upstream, analytical, and manufacturing teams.

• Maintain lab equipment, manage buffers and column inventory, and ensure compliance with safety and documentation standards.