Data University

Curriculum Vitae

Sudhakar Parthasarathy

Department of Chemistry, University of Illinois at Chicago

*** * ****** **, **** SES, Chicago, IL 60607

Phone: 312-***-**** email: **********@*****.***

Education

August 2006 – Current: PhD Candidate in Analytical Chemistry

June 2002: M.S Chemistry, University of Madras, India

June 2000: B.S Chemistry, University of Madras, India

Professional Experience

Aug 2006 – Current: Teaching and research Assistant, University of Illinois at Chicago

Jan 2003 – July 2006: Junior Scientist, Structure elucidation group, Analytical research, Dr.Reddy’s Laboratories Ltd., Hyderabad, India

Research Experience

Aug 2006- Till date

•Structural characterization of Amyloid β peptides Aβ(1-40) and Aβ(1-42) primarily by Solid-State NMR spectroscopy (SSNMR) using magic angle spinning (MAS) and cross-polarization (CP) 1D, 2D homonuclear dipolar recoupling experiments such as RFDR, DARR, heteronuclear double CP experiments and internuclear distance measurements from REDOR, CTfpRFDR experiments

•Molecular level metal (Cu2+/Zn2+) binding structures characterized by CP-MAS 1D, 2D and 13C, 15N and 1H paramagnetic relaxation (T1) experiments. Also used other methods like UV-VIS, fluorescence, circular dichroism and transmission electron microscopy for the characterization purposes

•Sensitivity enhancement in SSNMR by paramagnetic assisted condensed data collection method for nanomole scale protein micro crystals, amyloid fibrils and metallo proteins in hydrated and lyophilized conditions

•Have experience in handing first of its kind 1mm JEOL ultra high speed MAS probes (>70kHz) and 1.8mm high speed MAS probe (40kHz) . Handled 2.5mm and 3.2mm MAS probes frequently for routine analysis. Have knowledge in fixing solids probes.

•Have experience in working on 800MHz, 900MHz Bruker NMR instruments (Solids and Solutions equipped with cryo probe). Gained knowledge in setting up 1H detection experiments at ultra-high speed MAS (>70kHz). Implemented 1H detection pulse sequences with low power heteronuclear decoupling schemes

•Expertized in synthesizing, purifying and analyzing Aβ(1-40) and Aβ(1-42) peptides by solid phase peptide synthesis (SPPS) and in crystallizing proteins for SSNMR analysis using polyethyleneglycol (PEG) and methylpentanediol (MPD) precipitation. Have experience in hanging and sitting drop crystallisation methods

•Handled PC-12 Rat pheochromocytoma cells and performed toxicity assay experiments on these cells

Jan 2003- July 2006

•Structure elucidation of small organic molecules and their impurities in pharmaceutical products formed during drug discovery/process development/formulations/production using solution NMR experiments like DQCOSY, HSQC, HMBC, TOCSY, NOESY, HSQCTOCSY. Have experience in setting up LC-NMR experiments for impurity structural characterization

•Absolute configuration determination of chiral molecules using 1H and 13C solution NMR experiments

•Have good experience in interpretation of IR, UV-VIS, MALDI, electrospray, FT-MS, EI, CI mass spectral data

•Have sound knowledge in the solid-state characterization of drugs using Powder X-Ray diffraction, Thermal analysis Differential Scanning Calorimetry (DSC) and Thermo gravimetry (TG)

•Have grown single crystal x-ray diffraction quality small molecule organic crystals and co-crystals and also have experience in collecting x-ray diffraction data and solving the crystal structures.

•Written SOP and internal reports

Instrumentation experience

•Solid phase peptide synthesizer

•HPLC

•Single crystal X-Ray diffractometer

•Bruker/Agilent solution NMR 200-900MHz instruments

•Bruker/Agilent solid-state 400MHz NMR instrument (including Liq. N2 and He filling)

•UV-VIS spectrophotometer

•FT-IR spectrometer

•Fluorescence specrofluorimeter

•Polarimeter

•Circular Dichroism

•Elemental analyser

Computer skills

•Good programming skills in C/C++, worked in Unix and Linux Operating systems

Software skills

•NMR data acquisition and analysis programs (Topspin, XWINNMR, Spinsight, VNMR, NMRPipe, NMRDraw, Sparky)

•VMD

•Adobe Illustrator and Photoshop

Teaching Experience

•General, physical and analytical chemistry courses at University of Illinois at Chicago

•Trained colleagues and under graduate students in the industry as well as in academics

Awards and Honors

•University rank holder in MS, 2002

•ENC student travel award, 2009

•ISOTEC student Sponsorship award for ENC, 2010

•ISOTEC student Sponsorship award for ENC, 2011

•ISOTEC student Sponsorship award for ENC, 2012

Publications

•NMR Detection of Cyclic Redox Reactions in Cu2+-Bound Amyloid-b Fibrils Manuscript under preparation

•Resolution and Sensitivity Enhanced Solid-State NMR by SAIL Labeling and Ultra Fast MAS Manuscript under preparation

•Molecular level structural characterization of highly toxic A (1-42) amylo spheroids by by Solid-State NMR Spectroscopy Manuscript under preparation

•Molecular-Level Examination of Cu2+ Binding Structure for Amyloid Fibrils of 40-Residue Alzheimer’s β by Solid-State NMR Spectroscopy

Sudhakar Parthasarathy, Fei Long, Yifat Miller, Yiling Xiao, Dan McElheny, Kent Thurber, Buyong Ma, Ruth Nussinov, and Yoshitaka Ishii; J. Am. Chem. Soc., 2011, 133 (10), pp 3390–3400, February 22, 2011

•Nanomole-scale protein solid-state NMR by breaking intrinsic 1H T1 boundaries

Nalinda P Wickramasinghe, Sudhakar Parthasarathy, Christopher R Jones, Chhavi Bhardwaj, Fei Long, Mrignayani Kotecha, Shahila Mehboob, Leslie W-M Fung, Jaan Past, Ago Samoson and Yoshitaka Ishii; Nature Methods, Vol 6, 3, 215-218, 2009

•Solid state structural studies of saccharin salts with some heterocyclic bases

P. Sudhakar, S. Vijay Kumar, Peddy Vishweshwar, J. Moses Babu, K. Vyas; CrystEngComm, 2008,10, 996-1002

•Spectral Characterization of Itraconazole Impurities in the Drug Product

Nirmala Munigela, J.Moses Babu, Anjaneyulu Yerramilli, Sudhakar Parthasarathy, Vyas Krishnamurthy, K.N.Reddy; Trade science Inc, Analytical Chemistry, An Indian Journal, Vol. 7, Issue 2, 2008

•Identification and characterization of potential impurities of rabeprazole sodium

Ganta Madhusudan Reddy, B. Vijaya Bhaskar, P. Pratap Reddy, P. Sudhakar, J. Moses Babu K. Vyas, P. Ramachandra Reddy, K. Mukkanti; Journal of pharmaceutical and Biomedical analysis, Volume 43, 2007, 1262-1269

•Structural identification and characterization of potential impurities of pantoprazole sodium

Ganta Madhusudan Reddy, B. Vijaya Bhaskar, P. Pratap Reddy, S. Ashok, P. Sudhakar, J. Moses Babu K. Vyas, K. Mukkanti; Journal of pharmaceutical and Biomedical analysis, Volume 43, 2007, 1262-1269

•Carboxylic acid-pyridine supramolecular heterocatemar in a co-crystal

P. Sudhakar, R. Srivijaya, B.R. Sreekanth, P.K. Jayanthi, Peddy Vishweshwar, Moses J. Babu, K. Vyas, Javed Iqbal; Journal of molecular structure, 885, 2008, 45-49

•Synthesis and Crystal Structure of 2-(1-methyl-1H-3-indolyl) nicotinonitrile

Sudhakar Parthasarathy, Surajit Banerjee, Krishnamurthi Vyas, J.Moses Babu, Indu Dager and Manojit Pal; Analytical sciences: X-Ray structure analysis online, March 2006, Vol.22, x55.

•Identification and characterization of potential impurities of amlodipine maleate

P. Sudhakar, M. Nirmala, J. MosesBabu, K. Vyas, Ganta Madhusudan Reddy, B. Vijaya Bhaskar, P. Pratap Reddy, K. Mukkanti; Journal of pharmaceutical and Biomedical analysis, Volume 40, 2006, Issue 3, 605-613

•Structural studies of impurities of risperidone by hyphenated techniques

P. Sattanathan, J. Moses Babu, K. Vyas, R. B. Reddy, S.T. Rajan, P. Sudhakar; Journal of pharmaceutical and Biomedical analysis, Volume 40, 2006, Issue 3, 598-604.

•Identification and characterization of impurities in Racecadotril by LC-MS

K. Mallikarjun Reddy, J. Moses Babu, P. Sudhakar, M.S.P. Sharma, G. Sudershan Reddy, K. Vyas; Pharmazie, 61, 2006, 994-998

•Crystal Structure and Synthesis of 1-benzyl-4-(5,6-dimethoxy-1H-2-indenylmethyl) piperidine, a donepezil impurity

P. Sudhakar, Surajit Banerjee, K. Vyas, J. Moses Babu, E.R.R. Chandrashekar, P. Anil Kumar and Vijayavitthal T. Mathad; Analytical sciences: X-Ray structure analysis online, July 2005, Vol.21, Part 6, x97

Oral Presentations

•Molecular level Cu2+ binding structure of Aβ(1-40) and Aβ(1-42) fibrils and its red-ox reaction studied by SSNMR; Sudhakar Parthasarathy, 1st Baxter-UIC NMR exchange, 24th May 2011, Chicago, IL

•Solid-state NMR studies of Cu2+ binding to Alzheimer’s -amyloid fibrils; Sudhakar Parthasarathy and Yoshitaka Ishii; 51st Rocky Mountain conference on Solid State NMR, July 19-23, 2009 Snowmass, CO

•Cu2+ Binding Studies to Alzheimer’s -Amyloid Fibrils by Solid-state NMR; Sudhakar Parthasarathy and Yoshitaka Ishii; CANMRDG 2009, Baxter International Inc., Deerfield, IL, November 7th, 2009

•Crystal Structure of Quetiapine HCl; P. Sudhakar, J. Moses Babu, K. Vyas, Polavarapu Srinivas; Oral presentation, 35th National Seminar on crystallography, 22-24 February 2006, National Physical Laboratories, New Delhi, India

Poster Presentations

•NMR Detection of Cyclic Redox Reactions in Cu2+-Bound Amyloid-b Fibrils and Resolution Enhanced Solid-State NMR by SAIL Labeling and Ultra Fast MAS

Sudhakar Parthasarathy, Yusuke Nishiyama, Yuki Endo, Takahiro Nemoto, Masatsune Kainosho, Yoshitaka Ishii, 53rd Experimental NMR conference, April 15-20, 2012, Miami, Fl; 7th Annual Mid-west conference on protein folding, University of Notre Dame, IN

•Molecular-level Structure in Cu2+ Bound Aβ(1-40) Amyloid Fibril and its Redox Reaction Studied by Solid-State NMR and Molecular Dynamics Simulations; Sudhakar Parthasarathy, Yiling Xiao, Fei Long, Yifat Miller, Dan McElheny, Buyong Ma, Ruth Nussinov, and Yoshitaka Ishii; 52nd Experimental NMR conference, April 10-15, 2011, Asilamor Conference Grounds, Pacific Grove, CA; 6th Annual Mid-west conference on protein folding, University of Notre Dame, IN

•Metal-Binding Structure and Sensitivity Enhancement in Solid-state NMR studies of Cu2+ bound Alzheimer’s b-amyloid fibrils; SudhakarParthasarathy, FeiLong, Yiling Xiao, Dan McElheny, Yoshitaka Ishii; 51st Experimental NMR conference, April 18 - 23, 2010 at the Hilton Hotel Daytona Beach, Florida.

•Paramagnetic relaxation-assisted condensed data collection (PACC) for sensitivity enhancement in solid-state NMR and copper binding studies to Aβ(1-40) fibrils; Sudhakar Parthasarathy, Nalinda P. Wickramasinghe, Medhat A. Shaibat and Yoshitaka Ishii; 50th Experimental NMR conference, March 29 – April 3 2009, Asilomar Conference Grounds, Pacific grove, California; Society for applied spectroscopy (SAS) – Chicago, April 7, 2009, UIC, Chicago, Illinois; SES poster session, April 7, 2009, UIC, Chicago, Illinois; UIC Student Research Forum, April 17, 2009, Chicago, Illinois

•Accelerated Data Collection of Multi-Dimensional Solid-State NMR for Non-Paramagnetic Proteins in Microcrystals and Amyloid Fibrils by Paramagnetic Relaxation; Nalinda P. Wickramasinghe, Sudhakar Parthasarathy, Christopher R. Jones, Mrignayani Kotecha, and Yoshitaka Ishii; 49th ENC, March 9 - 14, 2008, Asilomar Conference Grounds, Pacific Grove, California

•Structural information and sensitivity enhancement for Aβ(1-40) intermediates and fibrils by solid-state NMR by paramagnetic relaxation; Medhat Shaibat, Sudhakar Parthasarathy, Nalinda Wickramasinghe, and Yoshitaka Ishii; Rocky mountain conference on Solid State NMR, July 27-31, 2008, Breckenridge, Colarado

•Crystal Structure of Amlodipine Maleate; P. Sudhakar, J. Moses Babu, K. Vyas, Ganta Madhusudhan Reddy; 35th National Seminar on crystallography, 22-24 February 2006, National Physical Laboratories, New Delhi, India

•Crystal Structure of Tizanidine Hydrochloride – an 2-adrenergic agonist; P. Sudhakar, J. Moses Babu, K. Vyas, M.N.V Brahmeshwara Rao, G. Srinivasulu; 35th National Seminar on crystallography, 22-24 February 2006, National Physical Laboratories, New Delhi, India

•Crystal Structure of Rutaecarpine; P. Sudhakar, J. Moses Babu, K. Vyas, V. Balasubramanian, Bipul Baruah;35th National Seminar on crystallography, 22-24 February 2006, National Physical Laboratories, New Delhi, India

•Structural Studies on the impurities of Esomeprazole using NMR Spectroscopy; P. Sudhakar, J. Moses Babu, K. Vyas, Santhosh. K. Deolia, Durvas N. Patil, A. Malleshwar Reddy and K. N. Reddy; Pharmacophore January 16-17, 2004, International Symposia conducted by Dr. Reddy’s Research Foundation, Hyderabad, India

References

•Professor. Yoshitaka Ishii,

Department of Chemistry & Director of UIC Center for Structural Biology,

University of Illinois at Chicago, 845 W. Taylor St., Chicago, IL 60607 USA,

******@***.***

•Professor. Leslie Fung,

Department of Chemistry, University of Illinois at Chicago,

845 W. Taylor St, Chicago, IL 60607 USA,

*****@***.***

•Professor. Tim Keiderling,

Department of Chemistry, University of Illinois at Chicago,

845 W. Taylor St, Chicago, IL 60607 USA

***@***.***

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