Quality Control Project

Name : Subha Eswaran

Qualification : M.Sc. (Biotechnology), B.Sc. (Microbiology), DISM

Computer familiarity : MS-Office, HTML, VB, Javascript, Java,Web Designing

Authorized to work in USA : Yes

Experience : 1 year

Educational Details:

Education Board/College/University % of

Marks

B.Sc.

(Microbiology) Bharathiyar University, Coimbatore, Tamil Nadu, India 70%

M.Sc.

(Biotechnology) Bharathidasan University, Trichy, Tamil Nadu, India 86%

Technical skills:

1. Microbial techniques:

Isolation, Characterization, culturing of microbes and developing pure cultures under aseptic condition, sterility testing, antibiotic assays, endo toxin assyas.

Aseptic techniques, Good laboratory practices.

Environmental sample collection and quality control and testing of samples, bio burden, membrane filtration.

Molecular biological techniques: Nucleic acid isolation, precipitation, purification, protein estimation,

Staining and measuring microbes Microscopy (Light,Bright field, Dark field, Fluorescent, Phase contrast, Electron microscope).

Quality control. Water, dairy and Food Microbiology – Plating techniques, MPN technique, Biochemical tests for Coliform identification and confirmation.

Extraction and purification of secondary metabolites,

2. Basic biochemical techniques, chromatographic techniques,

3. Genetic Engineering:

Gene isolation, transfer, fusion, cloning, synthesis & screening, mutagenesis.

Electrophoresis (Gel,SDS-PAGE,PAGE,PFGE), Blotting,Gel documentation.

PCR, RT-PCR

Gene transfer: Electroporation, chemical method of gene tranfer, Agrobacterium mediated gene transfer, Transformation, Transduction.

4. Immunological techniques: ELISA, EID, RIA, column chromatography, Immunological assay methods, blood grouping, VDRL, Widal tests, agglutination tests, antibody and antigen assays.

5. Plant tissue culture, Extraction and purification of metabolites.

6. Plant molecular virology.

7. Developing PCR, RT-PCR diagnostic kits.

Work Experience:

1. Designation : Lecturer

Institution : Muthayammal College of Arts and Science, Rasipuram, India (Affiliated with Periyar university, India).

Duration

Subject Handled (theory)

Practical’s Handled :

:

:

1 academic year (from June 2003 till April 2004)

Food microbiology, Genetics, Bio physics, Genetic engineering.

Microbiology and Immunology

Project Management : Isolation and characterization of calcium oxalate (Kidney stone) degrading Psuedomonas and isolating the concerned Plasmid and transferring to E.coli.

Description

Goal: To develop a living microbe as a therapeutic agent for kidney stone since it can circumvent the problems of pain, expense, need for skilled hands and repetitive administration.

Calcium oxolate (kidney stone) degrading microorganisms were isolated from the urinary soil sample and identified as Pseudomonas putida.

Using planting techniques and in half strength nutrient medium Pseudomonas putida has been isolated and characterized and checked for its ability to degrade calcium oxalate.

The plasmid prone character was confirmed and since Pseudomonas is pathogenic it is impossible to use it for therapeutic purposes.

Plasmid trasformed to non-pathogenic intestinal noraml flora E.coli, calcium oxalate degradation was confirmed further and trails have to be undertaken to develop it as therapeuitc agents.

Project guide : Subha Eswaran

Team : 6

Subjects handled (Theory) : Micorbiology, Biophysics, Immunology, Animal cell culture & technology.

Practicals handled : Microbiology, Immunology, Genetics.

2. Designation : Faculty

Institution : Aptech Computer Education, Namakkal, India.

Duration : 3 months

Subjects handled : MS-O, word, exel, power point, access

HTML

Project Experience (M.Sc

Project guide : Dr. S.K. Raj, Scientist F, Molecular Virology.

Institution : NBRI, Lucknow, India

Project 1 : Development of RT-PCR diagnostic kits

Project 2 : Development of dual viral resistance in tobacco plants against CMV & TyLCV by CPMR method using fused gene product.

Paper Presentation:

In National level biological conference ‘Biome and Biosciences’ conducted by Council for Scientific Industrial Research, India (CSIR) & Tamil Nadu State Council For Science and Technology, India (TNSCST) on January 10th 2004 on “Development of dual viral resistance in tobacco against CMV & TLCV by CPMR method using fused gene product”.

Training Attended:

Attended 1 week training in ‘Electrophoresis’ at Electrophoresis Institute, Yercaud, Tamil Nadu, India.

Techniques learned: Paper electrophoresis, Gel electrophoresis, PAGE, SDS- PAGE, Capillary electrophoresis,

2D gel electrophoresis, discontinuous gel electrophoresis, gradient gel electrophoresis, PFGE, Gel documentation.

Computer Skills:

Course : Diploma In System Management

Duratin : 1 year

Institution : Aptech Computer Education

Software learned : MS-O, HTML, Java, Java Script, VB, Front Office

Academic achievements:

10 th Grade : District first

M.Sc. (Biotechnology) : 5th rank in the Bharathidhasan University, Trichy, India.

Reference :

Dr.A.Mohan kumar Professor, Dept. of Zoology(aff. to Bharthiyar University)

Govt Arts and Science college, Truipur, Tamil nadu, India.

Ph: 011-91-984-***-****.

E-mail: *******@******.***

Dr. A.A. Mohamed Hatha

Associate Professor

Department of Marine Biology, Microbiology & Biochemistry

School of Marine Sciences

Kochi University of Science and Technology

Kochi

Kerala

E-mail: ************@*****.***

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