Shaozhao (Stan) Wang

Personal Information

A. Name: Shaozhao (Stan) Wang

B. Home Address: 2517-89 St. NW Edmonton, AB, Canada, T6K 2Y8

C. Tel. No.: 780-***-**** (home)

D. E-mail: w98ekf@r.postjobfree.com

E. Personal data: Canadian citizen, married, one son.

SUMMARY

A highly energetic organic chemist: has being working in Canadian industry for more than 5 years, has a good record on biotech R&D and project management; good at organic synthesis, chemicals isolation, purification and analysis; experience in biotransformation to achieve chiral and “green” products; biotransformation using whole cells of microbes, and downstream products recovery; QC methodology development; and experience in natural products and antibiotics, enzymes production R&D, technology transferring, process validation and academic & industrial collaboration.

TECHNICAL SKILL

Organic synthesis, biotransformations, chemoenzymatic synthesis routes design to achieve chiral and “green” products; chemicals recovery, purification, analytical and identification techniques: GC, Chiral-GC, HPLC, FT-IR, UV, LC-MS and NMR; Process scale-up skills, Microbial manipulation and enzymatic reactions; Knowledge about high throughput screening methods and Computer skills for data management, ChemDraw, and web-based databases.

EDUCATION

1999~2003: Doctor of Philosophy (organic chemistry), University of New Brunswick (Saint John, Canada)

1986 1989: Master of Science (organic chemistry), University of Zhongshan (Guangzhou, China)

1979~ 1983: Bachelor of Science (chemistry), University of Xiamen (Xiamen, China)

WORK EXPERIENCE

•10/2007 – 12/2011 – Research Scientist:

Afexa Life Sciences Inc. Edmonton, AB, Canada: Supervisor, Dr. Lei Ling (V.P.)

Responsible for directing and executing new products’ R&D, directs research associates and teammates in the initiation and execution of laboratory experimentation on discovery of new ingredients for Natural Health Product and botanical drugs, design the ingredient specification. Currently conduct two series of new products R&D: immuno-modulation and metabolic syndrome.

•01/2007 - 10/2007 – Research Scientist:

Biofine International Inc. Vancouver, BC, Canada: Supervisor, Dr. Wei Liu (President)

New products and contract projects R&D: planning, designing, implements and analyses; process scale-up and the final product quality control.

•07/2006 – 12/2006 - Guest Worker: Biotechnology Research Institute, NRC, Montreal Canada

Supervisor: Dr. Peter C. K. Lau, (Group Leader)

Joining the CBIN project on the biomass processing, cutinases application R&D, and new CHMO substrate profiling studies; Joining BioVision Technology Inc. (Nova Scotia, Canada) management team working on biomass pilot plant project, in STDC funding application: received the grant funding commitment for $ 3.0 million from STDC.

•07/2003 – 07/2006 - NSERC Fellowship: Biotechnology Research Institute, NRC, Montreal Canada

Supervisor: Dr. Peter C. K. Lau (Group Leader)

Responsible for carrying out biotransformations through new bioreagents, designing the products recovery, and chemical analysis by GC, GC-MS, LC-MS and NMR; designing and development of chemical screening methods for directed enzyme evolution and enzyme substrate profiling study.

•02/1999 – 08/1999 - Q. C. Technician: Glamour Look Ltd, Toronto, Canada

Supervisor: Ms. Jianping Zhao (MS, Lab manager)

Responsible for all income raw material inspection and recording, and laboratory management

•10/1995 – 11/1998 - Lecturer and Research Associate:

Institute of Microbiologic & Biochemical Engineering, Fujian Teachers’ University, Fuzhou, China

Supervisor: Shonggang Wu (Director General)

Responsible for WHMIS training and conducting research on developing a new process for isolated enzyme lipase from fermentations using various ultra-filtration systems; Conducting two subprojects in lipase R&D: A study of lipase immobilized on the solid support as biocatalyst; and the purification, characterization and N-terminal sequencing of the lipase from Penicillin expasum PF 868

•06/1989 – 09/1995 - Assistant Researcher: Fujian Institute of Microbiology, Fuzhou, China

Supervisor: Yuanrong Cheng (Director General)

Responsible for conducting research in extraction and purification of antibiotics, and establish the quality standards and quality control methods, and also exploring the semi-synthesis and modification of the antibiotics to obtain novel and improved drugs.

•06/1983 – 09/1986 - Assistant Teacher: Fujian Medical University, Fuzhou, China

Supervisor: Buyin Li (Sector Director)

Responsible for the chemical lab WHMIS training and chemistry experimental course, maintaining the chemical lab and reagents inventory update.

PUBLICATIONS:

Patent:

[1]. US Patent Application Publication, Shan et al. US2009/0285915A1, Nov 19, 2009.

Recent Conference Presentation:

S. Wang et al. “Methodologies of Ginseng Species Differentiation” 7th NHP Research Conference, Halifax, Nova Scotia, May 23rd-26th, 2010. (Speech)

Books (Chapter):

[1]. Practical Methods for Biocatalysis and Biotransformations, Chapter 11. Enzymatic Oxidation Chemistry; Ellis, A. J., Reiss, R., Snape, T. J., Turner, N. J., Pezzotti, F., Therisod, H., Therisod, M., Kaup, B.-A., Piantini, U., Wüst, M., Schrader, J., Chiappe, C., Colonna, S., Gaggero, N., Pellegrino, S., Zambianchi, F., Kirschner, A., Bornscheuer, U. T., Grogan, G., Hill, C., Wang, S., Yang, J. and Lau, P. C. K. (2009) Enzymatic Oxidation Chemistry, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/978**********.ch11

Journal Publication:

(1). Stephan Grosse, Helene Bergeron, Akihiro Imura, Jason Boyd, Shaozhao Wang, Kazuo Kubota, Akihiko Miyadera, Traian Sulea and Peter C. K. Lau;

Nature Versus Nurture in Two Highly Enantioselective Esterases Bacillus cereus and Thermoanaerobacter tengcongensis.

Microbial Biotechnology, (2010), 3(1): 65-73.

(2). Ahmad Mirza, Brahm J. Yachnin, Shaozhao Wang, Stephan Grosse, Helene Bergeron, Akihiro Imura, Hiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau and Albert M. Berghuis;

Crystal Structures of Cyclohexanone Monooxygenase Reveal Complex Domain Movements and a Sliding Cofactor.

Journal American Chemical Society, (2009), 131: 8848-8854.

(3). Jianzhong Yang, Shaozhao Wang, Marie-Josee Lorrain, Denis Rho, Kofi Abokitse and Peter C. K. Lau;

Bioproduction of Laury Lactone and 4-Vinyl Guaiacol as Value-added Chemicals in Two-phase Biotransformation systems.

Applied Microbiology and Biotechnology, (2009), 84(5): 867-876.

(4). Zhizhuang Xiao, Shaozhao Wang, Helene Bergeron, Jianchun Zhang, Peter Lau;

A Flax-retting Endopolygalacturonase-encoding Gene from Rhizopus oryzae.

Antonie van Leeuwenhoek, (2008), 94(4): 563-571.

(5). Jianzhong Yang, Yongqi Hu, Dishun Zhao, Shaozhao Wang, Peter C. K. Lau and Ian W. Marison;

Two-layer Continuous-process Design for the Biodesulfurization of Desel Oils under bacterial Growth Conditions.

Biochemical Engineering Journal, (2007), 37: 212-218.

(6). Hiroaki Iwaki, Shaozhao Wang, Stephan Grosse, Helene Bergeron, Ayako Nagahashi,

Jittiwud Lertvorachon, Jianzhong Yang, Yasuo Konishi, Yoshie Hasegawa, and Peter C.K. Lau;

Pseudomonad Cyclopentadecanone Monooxygenase Displaying an Uncommon Spectrum of

Baeyer-Villiger Oxidations of Cyclic Ketones.

Applied and Environmental Microbiology, (2006), 72(4): 2707-2720.

(7). Shaozhao Wang, Margaret M. Kayser, and Valdas Jurkauaskas. (2003)

Access to Optical Pure 4- and 5-Substituted Lactones: Chemical-Biocatalytic Cooperaction.

Journal of Organic Chemistry 68: 6222-6228.

(8). Shaozhao Wang, Margaret M. Kayser, Hiroaki Iwaki, Peter C. K. Lau. (2003)

Monooxygenase-Catalyzed Baeyer-Villiger Oxidations: CHMO vs CPMO.

Journal of Molecular Catalysis B: Enzymatic 22: 211-218.

(9). Shaozhao Wang, Gang Chen, Margaret M. Kayser, Hiroaki Iwaki, Peter C. K. Lau, and Yoshie Hasegawa. (2002)

Baeyer-Villiger Oxidation Catalyzed by Engineered Microorganisms: Enantioselective Synthesis of -Valerolactones with Functionalized Chains.

Canadian Journal of Chemistry, 80: 613-621.

(10). Hiroaki Iwaki, Yoshie Hasegawa, Shaozhao Wang, Margaret M. Kayser, and Peter C. K. Lau. (2002)

Cloning and Characterization of a Gene Cluster Involved in Cyclopentanol Metabolism in Comamonas sp. NCIMB 9872 and Biotransformations Effected by Escherichia coli-Expressed Cyclopentanone 1, 2-Monooxygenase.

Applied and Environmental Microbiology, 68(11): 5671-5684.

(11). Marko D. Mihovilovic, Gang Chen, Shaozhao Wang, Brian Kyte, Fernande Rochon, Margaret M. Kayser, and Jon D. Stewart. (2001)

Asymmetric Baeyer-Villiger Oxidations of 4-Mono- and 4,4-Disubstituted Cyclohexanones by Whole Cells of Engineered Escherichia Coli.

Journal of Organic Chemistry, 66: 733-738.

(12). Chen Xiulin, Zheng Yi, Wang Shaozhao, Shi Qiaoqin, Wu shonggang. (1999)

Studies of Lipase Immobilized on CM-Dextran Gel.

Pharmaceutical Biotechnology,(Chinese) 6(1): 20-23 (China, 9th-5 years project).

(13). Tang Lianghua, Wang Shaozhao, Shi Qiaoqin, Wu Shonggang. (1999)

Purification and N-Terminal Sequencing of the Lipase from Pencillium expansum PF 868.

Journal of Fujian Teachers University (Chinese) 15(4): 80-84 (China, 9th –5 years project).

(14). Chen Jianping, Wang Shaozhao, Wu Shonggang. (1998)

A Study on the Concentration of Alkaline Lipase by Using Various Ultrafilters. Food and Fermentation Industries (Chinese) 24(4): 9-11(China, 9th-5 years project).

(15). Shaozhao Wang, Chen Jianping, Wu Songgang. (1997)

Preliminary Study on the Technique of Oil Hydrolysis Catalyzing by Alkaline Lipases.

Pharmaceutical Biotechnology (Chinese) supplement editor, (9th-5 years project).

(16). M. N. Preobrazhenskaya, Shaozhao Wang, E. I. Lazhko, A. M. Korolev, and L. S.

Povarov. (1995)

Interaction of Anthracycline Antibiotics with Bis(trimethylsilye)- trifluoroacetamide.

Bioorg. Khim. (Russ) 21(11): 890-893 (Visiting Scholar).

(17). Xuguang Yan, Shaozhao Wang and Jianbo Wu. (1993) Isolation and Structure Determination of XF-1, A New Anthracycline Antibiotic.

Chinese Chemical Letters (Engl.) 4(9): 181-190 (Visiting Scholar)

(18). Huang Jinke, Wang Shaozhao, Wu Xuanchi. (1991)

The Study in Aza-Dieles-Alder Reaction of Aryl Imines with Cyclopentadiene.

Chinese Journal of Chemical Physics (Chinese) Vol. 4, No. 4, 302-312 (M.S. work).

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