Scientist / Manager Scientist

Xinyuan Duan Ph.D.

**** ****** ****** **, *******, CT 06810

Phone: 502-***-****

E-mail: ******@*****.***

Objective

Protein biochemist with 12 years of extensive skills and experience looking for an industry position where I can leverage my knowledge in biochemistry/ molecular biology/ microbiology and my capability in research and product development

Highlights

* 9 first-author publications in peer-reviewed journals, including Molecular Cell, Journal of Biological Chemistry, etc.

* 12 years of extensive experience in protein expression, protein purification, protein modification and protein assay.

* Strong personal and communication skills, exceptional independent research ability as well as great team worker.

* Supervisory experience

Laboratory Skills

* Protein Engineering:

Extensive experience in protein expression design in both prokaryotic and eukaryotic expression systems

Expression vector making--primer design, PCR, long PCR, RT-PCR, Sewing PCR, sub-cloning, site-directed mutation, restricted enzyme analysis, DNA and RNA purification;

Conversant with E.coli and baculovirus protein expression system; Extensive experience in development and maintenance of stable bacterial/insect cell lines

* Protein Purification:

Expert in natural protein and recombinant protein purification method development;

Extensive experience with various chromatography and electrophoresis techniques, such as Ion Exchange Chromatography, Size Exclusion Chromatography (SEC), Affinity Chromatography, hydrophobic interaction chromatography (HIC), SDS-PAGE, Native-PAGE, Isoelectric Equilibrium Electrophoresis (IEF), etc;

Hand-on experience with AKTA FPLC system;

* Protein assay/enzyme assay:

Extensive experience and knowledge in protein modification (chemical and enzymatic);

Conversant with protein assay development and various protein assay techniques, such as forced protein degradation, protein stability studies, Western Blot, ELISA, protein concentration determination, UV-Visible Spectrophotometer, protein-protein binding assay (eg ITC, Pull down), enzyme activity assay, enzyme kinetic assay;

* Statistics graduate courses taken: Probability, Biostatistical Method I, Biostatistical Method II (Statistical Linear Regression), Categorical Data Analysis, Mathematical Statistics, New Drug & Device Development, Survival Analysis, Epidemiology Methods II, Survival analysis, and Statistical Computational Data Management.

Education

Ph.D. Microbiology, 2004

State Key Laboratory of Microbial Technology

Shandong University, China

B.S. Microbiology, 1999

Shandong University, China

Research/Work Experience

2011-present Scientist, Medical Chemistry Department, Yoh Contractor to Boehringer Ingelheim Pharmaceuticals; Working on protein refolding, protein purification and protein characterization

2004-2011 Postdoctoral Scholar/Research Associate, James Graham Brown Cancer Center, University of Louisville; Studied the structure and function of Smc5/6 complex

* Defined the interaction region of Smc5 for Mms21 (Smc5 and Mms21 are two subunits of Smc5/6 complex); obtained crystals of the Smc5-Mms21 complex and solved the structure.

* Studied the function of Smc5-Mms21 complex in DNA damage repair and DNA replication

* Elucidated how the 8 subunits of Smc5/6 complex are able to bind each other using pull-down and yeast hybrid methods and verified protein NSE1 as a Ubiquitin E3 ligase

* Studied the interaction between SUMO E2-E3 complex

1999-2004 Graduate Research and Teaching Assistant, Shandong University

* Investigated the roles of several low-molecular-weight compounds involved in lignin degradation

* Studied regulation of cellulase gene expression in the filamentous fungus Trichoderma pseudokoingii S-38

* Developed a new method for isolating new cellulose degradation micro-organism from natural sample;

* Purified a new cellulase from mixed fermentations; studied biochemical and molecular characteristics of the new cellulase

1999-1999 Undergraduate Research Assistant, Shandong University

* Optimized Laccase producing conditions for Trametes versicolor, purified laccases from Trametes versicolor, identified some physical and chemical properties of laccase

Teaching Experience

6/2005 - 8/2005 Mentor to one summer student intern, University of Louisville

9/2002 - 6/2004 Thesis advisor to 1 graduate (MS) student and 4 undergraduate students, Shandong University.

2/2002 - 6/2002 Teaching assistant, Microbial Physiology & Genetics Lab, Shandong University, P. R. China

Internship

6/2001 - 8/2001 Shandong Xinhua Pharmaceutical Company, Shandong, P. R. China, Summer Research Fellow. Participated in clinical studies of an oral tablet (Glimepiride tablets) for diabetics with focus on drug dynamics research

6/1998 - 8/1998 Shandong Xinhua Pharmaceutical Company, Shandong, P. R. China. Documented quality control procedures and implementation instructions for a new product

Honors and Awards

* Fellowship Research Fund: “Structural Studies of SUMO E2-E3 Interaction” funded by Kentucky Lung Cancer Program, $38,000, 2007

* University Research Studentship 1999-2004

* Honored Student, Shandong University, 1999

Affiliation/Membership

* Member of American Association of Cancer Research (AACR)

* Member of the American Crystallography Association (ACA)

* Full Member of Sigma Xi (The Scientific Research Society)

Selected Publications

1. Xinyuan Duan, Prabha Sarangi, Xianpeng Liu, Gurdish K. Rangi, Xiaolan Zhao, Hong Ye. Structural and functional insights into roles of the Mms21 subunit of the Smc5/6 complex. Molecular Cell, 2009, 35(5), 657-668.

2. Yu-Hung Chen, Koyi Choi, Barnabas Szakal, Jacqueline Arenz, Xinyuan Duan, Hong Ye, Dana Branzei, Xiaolan Zhao. Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair. Proceedings of the National Academy of Sciences (PNAS), 2009, 106(50), 212**-*****.

3. Xinyuan Duan, Hong Ye. Purification, crystallization and preliminary X-ray crystallographic studies of the complex between Smc5 and the SUMO E3 ligase Mms21. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2009, 65(8), 849-852.

4. Duan X, Yang Y, Chen YH, Arenz J, Rangi GK, Zhao X, Ye H. The architecture of the Smc5/6 complex of S. cerevisiae reveals a unique interaction between the Nse5-6 subcomplex and the hinge regions of Smc5 and Smc6. Journal of Biological Chemistry, 2009, 284(13), 8507-8515.

5. Duan X, Trent JO, Ye H. Targeting the SUMO E2 conjugating enzyme Ubc9 interaction for anti-cancer drug design. Anti-Cancer Agents Medicinal Chemistry (Formerly 'Current Medicinal Chemistry - Anti-Cancer Agents'), 2009, 9(1), 51-54.

6. Duan XY, Gao PJ. Volumetric productivity improvement for endoglucanase of Trichroderma pseudokoingii S-38. Journal of Applied Microbiology, 2004, 96(4), 772-776.

7. Duan XY, Xin W, Gao PJ. The role of cellobiose in cellulose biological degradation. Microbiology, 2003, 30(5), 94-98.

8. Duan, XY, Wang W, Lu XM, Gao PJ. The role of several low-molecular-weight compounds involved in lignin degradation. Journal of Chinese Biotechnology, 2003, Vol.23, 51-56.

9. Duan XY, Lu XM, Wang W, Gao PJ. The study of laccase production conditions of Phanerochaete chrysosporium. Journal of Shandong University, 2002, Vol.37, 91-94.

10. Duan XY, Li Y, Gao PJ. Recent advances on molecular biology of lignin degradation. Journal of Cellulose Science and Technology, 2001, Vol.9, 51-58.

11. Yu R, Wang L, Duan X, Gao P. Isolation of cellulolytic enzymes from moldy silage by new culture-independent strategy. Biotechnology Letters, 2007, 29(7), 1037-1043.

12. Liu SY, Duan XY, Lu XM, Gao PJ. A novel thermophilic endoglucanase from a mesophilic fungus Fusarium oxysporum. Chinese Science Bulletin, 2006, 51(2), 191-197.

Selected Presentations/Posters

1. Xinyuan Duan, Hong Ye. Structural and functional insights into roles of the SUMO E3 ligase Mms21 subunit of the Smc5/6 complex. 2009 American Crystallography Association Meeting. ON, Canada, July, 2009.

2. Xinyuan Duan, Hong Ye. The role of smc5/6 in chromosome function. Cold Spring Harbor 2009 meeting: The Ubiquitin Family. NY, April 22-25, 2009.

3. Xinyuan Duan Gurdish Kaur Rangi, Hong Ye. The interaction studies between Smc5 and SUMO E3 ligase Mms21. 12th Annual Midwest DNA Repair Symposium. KY, May 15-16, 2010

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