peptide/protein analytical chemist

OBJECTIVE

An industry position in the area of biotechnology, biomaterial, protein chemistry, analytical chemistry, with the opportunity of personal and professional growth

SUMMARY OF QUALIFICATIONS

 Intensive experience in protein chemistry, protein analysis and functional modification

 Expert in de novo design of peptide/metalloprotein, and the stability and kinetics analysis of metalloprotein by spectroscopic methods

 Strong multidisciplinary background, especially in biochemistry, peptide chemistry, analytical chemistry, and photochemistry

 Extensive experience in analysis instruments operation and trouble shooting: GC, 1H NMR, 13C NMR, GC-MS, MALDI-TOF, DIP, HPLC, SDS-PAGE, isothermal calorimetry, gel electrophoresis, circular dichroism spectrophotometer, UV-vis spectrophotometer, steady-state and time-resolved fluorimeter, N2-dye laser, transient absorption spectroscopy, transient emission spectroscopy, Fourier Transform IR spectroscopy (FT-IR) and spectro-electrochemistry

 A motivated, creative, passionate and hard worker and a professional team player with strong problem solving and coordinating multiple projects abilities, strong communication, presentation and interpersonal skills

EDUCATION

 Ph.D. Candidate, Photochemical Science, BGSU, Bowling Green, OH, anticipated Oct 2009

Advisor: Professor Michael Y. Ogawa

Thesis title: “Bottom-up Design of the Synthetic Photoactive Metalloproteins”

 M.S., Organic Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, July 2003

Advisor: Professor Guoqiang Yang

Thesis title: “Synthesis and spectroscopic properties of sandwich-like bis(phthalocyaninato) europium(III) under normal condition and high pressure condition”

 B.S., Chemistry, Shandong University, Ji’nan, China, July 2000

Advisor: Professor Miao Zhang

Thesis title: ‘‘The synthesis of a series of photosensitive materials based on3-nitro-1-(2,4,6,trimethyl -phenyl)-4-bromine-2-pyrazolin-5-one’’

RESEARCH EXPERIENCE

2004-present Research Assistant, Bowling Green State University

 Designed a series of peptides with different oligomerization states and investigated the effects of the coordination of metal ions on their structures

 Created a miniature Cu(I) metalloprotein, studied the collisional electron-transfer in the inverted Marcus region and the effect of diffusion limit

 Developed a negatively charged metallopeptide probe base on ruthenium complex

 Investigated the electron transfer between the negatively charged metallopeptide and ferricytochrome c

 Designed a miniature real-time emission-potentiometry

2000-2003 Research Assistant, Institute of Chemistry, Chinese Academy of sciences, China

 Synthesized a series of subphthalocyanines and sandwich-like europium bis(phthalocyaninato) complexes

 Studied the photochemical and photophysical properties under ambient and high pressure condition

 Investigated the mechanism of increased quantum yield under high pressure condition

1999-2000 Research Assistant, Sanxing Company, Shandong University

 Synthesized a series of photosensitive materials and investigated their spectroscopic properties

INTERNSHIP EXPERIENCE

Synthesis of drug-like intermediates and molecules May, 2009-August, 2009 CGene Tech Inc.

WORKING EXPERIENCE

2003-2004 Neotrident Company (Beijing, China)

 Co-developed the China Natural Products Database (CNPD)

AWARDS

 “Chinese Student Academic Awards in BGSU”, 2005

 “Outstanding Student Scholarship of Shandong University ” 1997-1999

 “Distinguished Student Scholarship of Shandong province” 1996

AFFLILIATION

 American Chemistry Society Member since 2005

PUBLICATION

 Fan, J.; Daniil Zaytsev ; Ogawa, M. Y. “Identifying important structural characteristics of Cadmium proteins by de novo designed coiled coils” In preparation

 Fan, J.; Ogawa, M. Y. “Effect of Protein Structure on the Driving Force Dependence of Bimolecular Electron-transfer Rates” In preparation

 Liu, L.; Hong, J.; Fan,J.; Ogawa, M. Y. “Observation of True Electron-transfer Rates within Dynamic Peptide-Protein Complexes” In preparation

 Ogawa, M. Y.; Fan, J; Fedorova, A.; Hong, J.; Kharenko, O. A.; Kornilova, A. Y.; Lasey, R.C.; Xie, F. “Electron-transfer functionality of synthetic coiled-coil metalloproteins” J. Braz. Chem. Soc. 2006, 17(8), 1516-1521 (Invited review paper).

 Hong, J.; Kharenko, O. A.; Fan, J.; Xie, F.; Petros, A. K.; Gibney, B. R.; Ogawa, M. Y. “Evidence that a Miniature Cu(I) metalloprotein Undergoes Collisional Electron Transfer in the Inverted Marcus Region.” Angew. Chem. Int. Ed. 2006, (37), 6137-6140.

 Liu, D.; Zhang, J-L.; Fan, J.; Han, B-X, Chen, J. “Effect of CO2 on the Properties of AOT/Water/Isooctane Reverse Micelles by Fluorescence Study” J. Phys. Chem. B 2004, 108(9), 2851-2856.

 Fan, J.; Li, H.; He, L-M; Xiong, F.; Li, S-Y; Wang, S-Q; Shen, S-Y; Xu, H-J; Yang, G. “High Pressure Effect on Spectroscopy Properties of Sandwich-like Europium bis[2,3,9,10,16,17,23,24-octakis(4-methoxyphenoxy)phthalocyninato]” Acta Chimica Sinica 2004, 62(1), 37-41.

 He, L.; Li, H.; Fan, J.; Li, S.; Gan, Q.; Zhang, G.; Zhang, B.; Li, Y.; Yang, G. “High Pressure Effect on the Intramolecular Electron Transfer Process” Chem. Phys. Lett. 2003, 378(34), 263-268.

 Liu, D.; Zhang, J.; Han, B-X; Fan, J.; Mu, T.; Liu, Z.; Wu, W.; Chen, J. “Effect of Compressed CO2 on the Properties of AOT Reverse Micelles Studied by Spectroscopy and Phase Behavior” J. Chem. Phys. 2003, 119(9), 4873-4878.

 Hu, J; Sui, Z.; Lu, H.; Qin, H.; Fan, J.; Wang, J.; Liu, Q. “Nanoparticles TiO2 and Photo-catalyst Degradations of Biological Dyes” Gongneng Cailiao 2003, 34(3), 336-337.

PATENT

 Hu, J.; Sui, Z.; Qin, H.; Lu, H.; Fan, J. “Preparation of nickel nanoparticles” Faming Zhuanli Shenqing Gongkai Shuomingshu China Patent CN20011007728 20010109 (2001).

POSTER & PREAENTATIONS

 Fan, J; Ogawa, M. Y. “The effect of protein structure in regulating biomolecular photoinduced electron-transfer rates in designed metalloproteins” 39th Central Regional Meeting of the American Chemical Society, Covington, KY, United States, May 20-23, 2007

 Fan, J; Ogawa, M. Y. “The effect of protein structure in regulating bimolecular photoinduced electron-transfer rates in designed metalloproteins” 233rd ACS National Meeting, Chicago, IL, United States, Mar. 25-29, 2007

 Fan, J; Hong, J.; Ogawa, M. Y. “Spectra and Electron Transfer Study of A Miniature Cu(I) Metalloprotein in the Inverted Marcus Region” Center for Photochemical Sciences Advisory Board Meeting Bowling Green, OH, United States ,Oct. 17-18, 2006

 Fan, J, Zhong, B.; Yang, G. “high pressure effects on the luminescence and crystalline properties of the complex of 2-(2’-hydroxyphenyl) oxazole with zinc” The First Asian Conference on High-Pressure Research, (ACHPR) Dunhuang, China, Aug. 20-25, 2002

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