Quality Control Technician

AUDREY NGUYEN

714-***-**** *************@*****.***

CAREER OBJECTIVE:

Seeking an opportunity to work as a Histotechnician.

LAB SKILLS:

• Fixation, processing, embedding, sectioning/cutting, and staining biopsies and tissue specimens.

• Specimens such as bone marrow, lymph node, gastrointestinal, breast, liver, lung, prostate, cervix, ECC, derm (skin), nails, bones, vas deferens, tonsil, and more.

• Performing Immunohistochemistry. ER, PR, Hercep, EGFR, Kappa/Lambda-ISH, CD markers, Cytokeratins, Ki67, P53, P27, HP (Helicobacter Pylori), Triple Stain, and more.

• Antigen and epitope retrieval.

• Routine and special staining on biopsies and tissue specimens manually and using automated stainers. Stainings include: routine H&E; and special stainings Alcian Blue, PAS, dPAS, Wright Giemsa, Iron Stain, Colloidal Fe, AFB, GMS, Reticulin, Trichrome, Mucicarmine, Acid Fast, EVG, Gram, Congo Red, Feulgen Stain, MPO, and more.

• Frozen sectioning.

• Minimizing the facing of the block when necessary.

• Prepared reagents (diluents) and wash buffers.

• Cover slipping slides manually.

• Grossing bone marrows.

• Imaging slides.

• Performing DNA analysis using computer software application.

• Quality control.

• Using Windows XP/Me/2000/NT/Vista and Microsoft Office 1997/2003/2007.

• Interacting and communicating with staff and pathologists, and assisting them.

• Compliant to Rules and Regulations.

SKILLS IN OPERATING AND MAINTAINING LABORATORY EQUIPMENTS:

• Sakura Tissue-Tek V.I.P. processor and Leica Tissue processor.

• Sakura Tissue-Tek embedding station.

• Sakura and Leica microtomes.

• Leica Bond automated IHC staining.

• Ventana Benchmark XT automated IHC/ISH slide staining system.

• Ventana NexES automated IHC slide staining system.

• Ventana NexES Special Stains automated slide stainer.

• Leica, Sakura, and DakoCytomation autostainers.

• Dako Artisan special stains.

• Water bath.

• Decloaker.

• pH Meter

• Centrifuge.

• Sakura Finetek Tissue Tek automated cover slipping machine.

• Aperio Scanscope for imaging tissue.

CERTIFICATION:

ASCP (American Society for Clinical Pathology) Certified Histology Technician (CM)

No. 20030 State Issued: Illinois

Date Acquired: 11/2006

Expiration Date: 11/2012

EDUCATION:

• California State University, Long Beach BS Computer Science

• Rancho Santiago College AS Chemistry, AS Pharmacy

WORK EXPERIENCE:

Quest Diagnostics, Nichols Institute, San Juan Capistrano, CA

HISTOTECHNOLOGIST I 11/10–10/11

• Processed, embedded, sectioned, and stained patients’ tissue specimens and biopsies.

• Tissue specimens and biopsies included bone marrows, lymph nodes, prostates, skins, and more.

• Performed Immunohistochemistry. CD markers, Cytokeratins, HP, Kappa/Lambda-ISH, ER/PR, Ki67, EGFR, and more.

• Antigen and epitope retrieval.

• Stains included routine H&E and special stains Wright Giemsa, Iron Stain, Trichrome, Congo Red, Reticulin, GMS, AFB, MPO (Myeloperoxidase), Mucicarmine, Alcian Blue, PAS, and more.

• Grossing bone marrows.

• Cutting client’s blocks by adjusting the block holder on microtome to minimize facing the block too deep.

• Shaving blocks at 10 and 50 microns.

• Quality control. Troubleshooting.

Torrance Memorial Medical Center, Torrance, CA

ASCP CERTIFIED HISTOLOGY TECHNIICIAN II 7/09 –11/10

• Processed, embedded, sectioned, and stained patients’ tissue specimens and biopsies.

• Tissue specimens and biopsies included gastrointestinals, breasts, livers, prostates, derms (skins), bones, lymph nodes, bone marrows, renals, ECC, cervix, and more.

• Stains included routine H&E and special stains HP, Alcian Blue, PAS, dPAS, ABP (Alcian Blue PAS), Iron Stain, Trichrome, Reticulin, GMS, AFB, Mucicarmine, Acid Fast, Wright Giemsa, EVG, Congo Red, Gram, and more.

• Frozen sectioning.

• Quality control. Troubleshooting.

West Dermatology, Placentia, CA

ASCP CERTIFIED HISTOLOGY TECHNIICIAN (PER DIEM) 7/09 –11/10

• Processed, embedded, sectioned, and stained patients’ tissue specimens, which were derms (skins) and nails.

• Stains included routine H&E and special stains PAS and dPAS.

• Quality control. Troubleshooting.

Segane LLC, Costa Mesa,CA

WEB DEVELOPER (CONTRACT) 8/07–4/09

• Developed and maintained website according to the specification for financial business.

Los Alamitos Medical Center, Los Alamitos, CA

HISTOLOGY TECHNICIAN 3/06 – 6/07

• Fixed, processed, embedded, sectioned, and stained patients’ tissue specimens and biopsies.

• Tissue specimens and biopsies included gastrointestinals, breasts, prostates, skins, ECC, cervix, bones, and more.

• Stains included routine H&E and special stains Alcian Blue, Wright Giemsa, Iron Stain, Trichrome, PAS, dPAS, Mucicarmine, and Acid Fast.

• Assisted pathologists in frozen sectioning.

• Cytology.

• Manual cover slipping.

• Quality control.

US Labs Accupath Diagnostics, Irvine, CA

LAB AND IMAGE TECHNICIAN 10/03 –7/05

• Fixed, processed, embedded, sectioned, and stained patients’ tissues and biopsies.

• Patients’ tissues and biopsies included breasts, lung, prostates, and more.

• Stains included routine H&E and special stain Fuelgen stain.

• Performed Immunohistochemistry. ER, PR, Herceps, Ki67, P53, P27, and more.

• Antigen and epitope retrieval.

• Quality control.

• Imaged slides using Aperio.

• DNA analysis.

• Prepared patients’ paperwork and slides for Pathologists.

REFERENCES:

• Joyce Tonui 909-***-****

• Dana Contis 310-***-****

• Dr. Ehsan Gharadjedaghi 949-***-****

Contact this candidate