Analytical Chemist

Zamir H. Warsi

*** ***** **** ******, ******, California 92324-6834

Home: 909-***-**** • ***********@*****.***

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Professional Experience

Immunochemical, Biochemical, Radioimmunoassay, Immunoassays, PCR, SDS-PAGE, ELISA, Immunohistochemistry, Immunocytology, Tissue culture (Mammalian cell culture), Cytogenetic FISH, FACS (Immunoflouresence), Antigens/ Antibodies production and purification, Affinity column chromatography, PH and salt gradients, Chromato-Isoelectro focusing, Protein concentrations, Immunoelecrtophoresis, Iodination (Isotopes), Animal immunization and surgery (Mice, Rat, Hamster, Guinea Pig, Rabbit, Goat), and Cell separation.

Children Hospital Los Angeles - Los Angeles, California (May 1990 – January 2009)

Department of Pathology (January 2007 - January 2009)

Tissue Culture Technologist

Specific tumor cell lines cultured from fresh patient tissues obtained from OR using pure tumor cells and stored in the clinical database, develop new methods. Total of 96 tumor cell lines including Brain tumors, Retinoblastoma, Ewing’s Sarcoma and other solid tumors have been developed.

Tissue banking: Freeze/thaw tissues & cells; develop specific cancer cell lines from fresh tumor cells separated from bone marrow and tissues.

Cryostat sectioning for DNA/RNA extraction and stain tissue sections for H&E slides.

Maintaining frozen tissues and cell lines banking and fill all research requests.

Maintaining lab equipment and ordering supplies.

Updating database of pure cancer cell lines and tissues

Department of Pediatrics, Division of Hematology-Oncology (January 2000 – January 2007)

Research Specialist III

Independently maintaining all histology staining and the training of new hires.

Developed new methods for routine and non-routine staining for clinical research.

Tissue culture, maintaining cell lines and tissue banking.

Staining bone marrow and cell lines for neuroblastoma.

Purifying DNA from cryostat tissue sections for N-myc amplifications.

Gene amplification using PCR techniques.

Separate mononuclear cells from normal blood donors and isolate NK and NKT cells to propagate in culture for injections

Department of Molecular Diagnostics/Pathology (October 1996 – January 2000)

Research Specialist III

Follow standard methods and develop new methods (30` hybridization of DNA probes for N-myc, XX/XY, EWS, and HER-2 New Chromosomes on Bone-Marrows and fresh tissues, 4-6 hours hybridization for other probes including paraffin tissues), report results and order laboratory supplies, trouble shoot.

Maintaining tissues culture, cell harvesting on variety of tissue specimens (neonatal, prenatal, postnatal blood and bone marrows) FISH testing, confirmatory FISH, microscope analysis and continuous improvement of laboratory procedures and equipment maintenance.

Department of Pediatrics, Division of Hematology-Oncology Department (May 1990 – October 1996)

Research Specialist II

Develop new methods for routine and non-routine staining for clinical research.

Purify DNA from cryostat tissue sections for N-myc amplification.

Independently maintaining all histology staining.

Gene amplification using PCR techniques.

Training of new laboratory personnel.

City of Hope – Duarte, California

Department of Anatomic Surgical/Pathology (April 1984 – May 1990)

Research Assistant II

Provide technical support, communication, interpersonal and organizational skills to help build a research and diagnostic program. Immunohistochemistry methods.

Design and perform assays and tests for frozen and paraffin embedded tissues for research and clinical diagnosis.

Tissue culture, production of new antibodies for cancer diagnostics.

Provide services to test new antibodies from different organizations before they are commercially available.

University of Manitoba, Canada

Department of Immunology (September 1982 – September 1983)

Research Associate III

Immunochemical and radioimmunoassay isolation of immunosupressor and stimulatory factors from rats and mice salivary gland extracts.

Column/gradient/affinity chromatography, chromatofocusing, isoelctrofocusing, RIA, ELISA and SDS-PAGE electrophoresis.

D.A. Stewart Respiratory Center (August 1980 – September 1982)

Senior Research Associate IV

Performed immunochemical, immunobiological, radioimmunoassay for total serum IgE (PRIST) and serum antibodies to common allergens (RAST).

Prepare extracts of local allergenic materials and develop RIA for common environmental allergens which are not commercially available, (Farmers lung disease).

Department of Immunology (August 1974- August 1980)

Research Associate IV

Production of Antibodies, Isolation and purification of Antigens Antibodies.

Affinity column chromatography, pH and salt gradients, chromatofocusing, isoelectrofocusing, protein concentrations, immunoassays and SDS-PAGE electrophoresis.

Activation of Gels, paper discs, Iodination and use of radioactive materials.

Tissue culture and cell separation, use of animal models, Immunization, tumor growth in vivo, Immunoassays and ELISA tests.

Certificates and Training

The Association of Genetic Technologists Inc.

Successfully Completing the Requirements for Spectra-Vision Wet-Lab Workshop, and training

Provided by Vysis Issued on October 26, 1998 Downers Grove, Illinois 605

Certificate of Training for the Introduction to Molecular Cytogenetics

Issued on November 15, 1996 by Oncor, Marryland, Dulles

American Society for Quality Control

Certified as a Member, Issued on March 14, 1983

P.O. Box 3005, Milwaukee, Wisconsin, 53201

Education

London Metropolitan University (formerly Polytechnic University of North London) London, England. Courses in Analytical Chemistry (MS).

University of Karachi – Karachi, Pakistan Bachelor of Science Degree

Dual Major: Chemistry and Zoology

Publications

Metelista LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.

J Exp Med. 2004 May 3; 199(9):1213-21.

Esteban JM, Warsi Z, Hanau M, Hall P, Shively JE, Chen S. Detection of intratumoral aromatase in breast carcinomas. An immunohistochemical study with clinicopathologic correlation. Am J Pathology. 1992 Feb; 140(2):337-43.

Esteban JM, Battifora H, Warsi Z, Bailey A, Bacus S. Quantification of estrogen receptors on paraffin-embedded tumors by image analysis. Mod Pathology. 1991 Jan; 4(1):53-7.

Berczi I, Holford-Strevens V, Warsi ZH, McMorris LS, Thorlakson RH, Thorlakson TK, Sehon AH. Tumor-reactive IgE antibodies in plasma of patients with gastrointestinal carcinomas. Cancer Immunology, Immunotherapy. 1983; 14(3):180-4.

References

Provided upon request

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