Project Development

ARUNA RAYAPEDDI

**** *** ****** **** ~ Mclean, VA, 22102

571-***-**** *********@*****.***

SUMMARY OF QUALIFICATIONS

Recent graduate of Johns Hopkins University with an M.S. in Biotechnology, Passionate and accomplished Biotech/Pharmaceutical researcher having a varied and extensive experience in molecular biology, cell culture and biochemistry techniques, commitment to continuous learning, Supportive team player, Detail oriented and well organized. Experienced and skilled presenter, motivational and a thoughtful instructor with Seven Years of experience teaching Biology at the University level.

EDUCATION

JOHNS HOPKINS UNIVERSITY, Krieger School of Arts and Sciences Rockville, MD

Master of Science in Biotechnology - GPA: 3.63 December, 2011

SRI VENKATESWARA UNIVERSITY, Tirupati Andhra Pradesh, India

Master of Science in Botany - GPA: 4.0 1997

Bachelor of Science in Botany, Zoology and Chemistry - GPA: 3.83 1995

RELEVANT EXPERIENCE

Volunteer Researcher Dec 2011 - Present

Laboratory of Biochemistry and Molecular Biology (LBMB), National Cancer Institute (NCI), NIH Bethesda, MD

Project: Telomere Capping Protein Complex

• Supervised by Dr. Yikang Rong.

• Stn 1/Ten 1 are the Telomere protecting proteins. Assist to clone a Vector to express Flag - Stn 1 and HA - Ten 1 proteins, purify proteins using affinity chromatography techniques and analyze them using Western blotting.

• Assist in 'Recombineering' to construct Stn 1 / Ten 1 mutation vectors for Fly injection.

• Contribute to the better understanding of how eukaryotic organisms maintain the physical integrity of their genomes.

• Maintain good written records of laboratory procedures, results and conclusions.

• Ability to work independently under minor supervision.

Laboratory Activities Related to Project

Molecular Biology, Cell Culture & Biochemistry techniques:

• Cloning Genomic DNA

• Plasmid DNA purification--Miniprep & Midiprep

• Quantification of DNA

• Agarose Gel electrophoresis

• Gel Purification

• Restriction Digestion

• Ligation

• Bacterial Transformation • PCR

• Single Fly PCR , Colony PCR

• Protein Gel electrophoresis (SDS-PAGE)

• Western Blot

• Affinity Chromatography

• ELISA

• Recombineering

• Aseptic techniques

• Cell culture techniques

LABORATORY SKILLS

MOLECULAR BIOLOGY & BIOCHEMISTRY TECHNIQUES: Cloning Genomic DNA | Quantification of DNA | Plasmid DNA purification | Agarose Gel electrophoresis | Protein Gel electrophoresis (SDS-PAGE) | Western blot | DNA Digestion | Ligation | E-coli transformation | Conventional PCR | Quantitative Real time PCR | Colony PCR | RNA isolation, analysis | ELISA | Recombineering | Affinity Chromatography techniques.

CELL CULTURE TECHNIQUES:

Mammalian Cell culture | Insect Cell culture | Plant Tissue culture | Transfection | Cell Toxicity Screening Assays (MTS and LDH) | Seed train development | Process development with SF-9 and Hybridoma cells and small scale bio-reactors (stirred tank bio-reactor, and wave bag bio-reactor).

OTHER EXPERIENCE

GURUKUL COLLEGE, Bangalore, India 2002 - 2004

Lecturer/Instructor Biology & Botany

M.N.R. COLLEGE, Hyderabad, India 1998 - 2001

Lecturer/ Instructor Biology & Botany

RISHI DEGREE COLLEGE, Hyderabad, India 1997 - 1998

Lecturer/Instructor Biology & Botany

• Taught 300+ undergraduates and positively influenced their appreciation of Botany & Biology.

• Related fundamental biology concepts to real world problems to aid students grasp of the subject matter.

• Collaborated with colleagues on strategies to keep students motivated and engaged.

• Assisted in the creation of ancillary cooperative learning activities giving students the opportunity to achieve a level of subject matter mastery and improve their presentation skills.

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