Protein Purification Scientist
Resume:
Tony S. Byun
****.****@*****.***
Brisbane, CA 94005
EDUCATION
Doctor of Philosophy, Molecular Pharmacology, February 2005
Stanford University School of Medicine, Stanford, CA
Bachelor of Science, Biochemistry, June 1996
University of California at Davis, Davis, CA
Graduated with High Honors
RESEARCH EXPERIENCE
Catalyst Biosciences South San Francisco, CA
Staff Scientist II (promoted), Protein Sciences 2005-April 2012
• Responsible for protein purification development and production for four biopharmaceutical programs (2 currently)
• Played key role in Factor VIIa program that led to a clinical candidate nomination in 1 year (clinical candidate and back-up compounds were partnered with Pfizer)
• Developed protein expression and purification processes for the Factor VIIa program (mammalian system)
• Developed automated protein purification methods for the various programs on either the AKTA Pilot, Purifier or Express
• Refolded and purified hundreds of protease variants from inclusion bodies expressed in E. coli for both in vitro and in vivo studies
• Responsible for LC/MS characterization of drug candidates to confirm identity and to characterize post-translational modifications
• Developed methods to evaluate post-translational modifications of Factor VIIa by IEF and HPLC (IEX)
• Expressed and purified proteases from P. pastoris
Stanford University School of Medicine Stanford, CA
Predoctoral Fellow/Ph.D. Research 2000-2005
Laboratory of Karlene Cimprich, Department of Molecular Pharmacology
Dissertation: Replication-dependent Activation of the DNA Damage Checkpoint
• Identified a novel mechanism by which cells sense the presence of lesions on DNA using Xenopus egg extracts
• Expressed and purified proteins in E. coli, insect (baculovirus system) and mammalian cells
• Expressed and purified monoclonal antibodies
• Performed transfections and immunofluorescence experiments in mammalian cells
• Performed loss of function experiments in mammalian cells using siRNA
• Proficient in current molecular biology techniques
• Supervised graduate students
Novozymes Biotech Inc. (formerly Novo Nordisk Biotech) Davis, CA
Research Associate (promoted), Department of Protein Chemistry 1996-2000
• Purified numerous proteins to homogeneity from crude extracts using conventional chromatography methods (size exclusion, ion exchange, hydrophobic interaction, affinity chromatography) by FPLC
• Purified milligram to gram quantities of recombinantly expressed proteins
• Performed enzyme characterizations by reverse-phase HPLC and GC
• Performed enzyme kinetics studies on metalloproteases
• Performed extensive biochemical characterization of enzymes for patents and for peer-reviewed publications
• Provided protein chemistry support to other departments within the company
• Supervised many undergraduate interns
University of California, Davis Davis, CA
Undergraduate Research 1994-1996
Laboratory of Kristnan Nambiar, Department of Chemistry
Honors Thesis: Chemical Synthesis of a Synthetic Protease
• Synthesized chemical compounds using standard organic synthesis techniques
• Purified organic compounds by TLC, crystallization and flash chromatography
• Performed analysis of organic compounds by TLC, NMR, and IR
U.S. PATENTS
Co-inventor
7,279,308 Polynucleotides encoding polypeptides having aminopeptidase activity
7,098,001 Polypeptides having dipeptidyl aminopeptidase activity and nucleic acids encoding same
6,800,467 Polypeptides having aminopeptidase activity and nucleic acids encoding the same
6,682,922 Polypeptides having dipeptidyl aminopeptidase activity and nucleic acids encoding same
6,673,571 Polypeptides having aminopeptidase activity and nucleic acids encoding the same
6,664,092 Polypeptides having dipeptidyl aminopeptidase activity and nucleic acids encoding same
6,489,154 Polypeptides having lysophospholipase activity and nucleic acids encoding same
6,465,209 Methods of producing protein hydrolysates
6,303,360 Polypeptides having aminopeptidase activity and nucleic acids encoding same
6,184,020 Polypeptides having aminopeptidase activity and nucleic acids encoding same
U.S. PATENT APPLICATIONS
Co-inventor
200******** Polypeptides having aminopeptidase activity and nucleic acids encoding same
200******** Polypeptides having aminopeptidase activity and nucleic acids encoding same
200******** Polypeptides having dipeptidyl aminopeptidase activity and nucleic acids encoding same
200******** Polypeptides having phospholipase activity and nucleic acids encoding same
200******** Methods for producing coagulated whey protein
200******** Polypeptides having aminopeptidase activity and nucleic acids encoding same
PUBLICATIONS
Academia
MacDougall CA, Byun T, and Cimprich KA. 2007. Structural Determinants of Checkpoint Activation. Genes & Development. 21(8): 898-903
Byun T, Pacek M, Yee MC, Walter JC, Cimprich KA. 2005. Functional Uncoupling of MCM Helicase and DNA Polymerase Activities Activate the ATR-dependent Checkpoint. Genes & Development. 19(9): 1040-1052
Lupardus P, Byun T, Yee MC, Hekmat-Nejad M, Cimprich KA. 2002. Requirement for Replication in Activation of the ATR-dependent DNA Damage Checkpoint. Genes & Development. 16(18): 2327-32.
Industry
Langston J, Blinkovsky A, Byun T, Terribilini M, Ransbarger D, Xu F. 2007. Substrate Specificity of Streptomyces Transglutaminases. Appl Biochem Biotechnol. 136(3):291-308
Byun T and Blinkovsky AM. 2004. Glycyl Aminopeptidase (Sphingomonas). Handbook of Proteolytic Enzymes, 2nd ed. 470-471.
Xu F, Byun T, Dussen HJ, Duke KR. 2003. Degradation of N-Acylhomoserine Lactones, the Bacteril Quorum-sensing Molecules, by Acylase. J Biotechnol. 101(1): 89-96.
Byun T, Tang M, Sloma A, Brown KM, Marumoto C, Fujii M, Blinkovsky AM. 2001. Aminopeptidase from Sphingomonas Capsulata. J Biol Chem. 276: 17902-7.
Byun T, Kofod L, Blinkovsky AM. 2001. Synergistic Action of an X-prolyl Dipeptidyl Aminopeptidase and a Non-specific Aminopeptidase in Protein Hydrolysis. J Agric Food Chem. 49: 2061-3.
Blinkovsky AM, Byun T, Brown KM, Golightly EJ, Klotz AV. 2000. A Non-specific Aminopeptidase from Aspergillus. Biochem Biophys Acta. 1480: 171-81.
Blinkovsky AM, Byun T, Brown KM, Golightly EJ. 1999. Purification, Characterization, and Heterologous Expression in Fusarium venenatum of a Novel Serine Carboxypeptidase from Aspergillus oryzae. Appl Environ Microbiol. 65(8): 3298-303.
Berka RM, Rey MW, Brown KM, Byun T, Klotz AV. 1998. Molecular Characterization and Expression of a Phytase Gene from the Thermophilic Fungus Thermomyces lanuginosus. Appl Environ Microbiol. 64(11): 4423-7.
PRESENTATIONS
Presented talk at Eukaryotic DNA Replication 2003 September 2003
Single-stranded DNA is an Integral Intermediate Involved in Activation of the ATR-mediated DNA Damage and Replication Checkpoints
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Presented poster at ASCB 42nd Annual Meeting December 2002
Requirement for Replication in Activation of the ATR-dependent DNA Damage Checkpoint
The American Society for Cell Biology, San Francisco, CA
Contact this candidate