Medical Technologist, (ASCP)
Resume:
Karen L. Johnson, M.T. (ASCP)
Smyrna, Georgia 30082
Goal: To join a laboratory team that performs clinical assays and provides quality results for patient care. I have years of experience both as a technologist and supervisor in the more esoteric disciplines of oncology hematology, flow cytometry, tissue typing, viral load testing using endpoint and realtime PCR assays, viral genotyping, genomic DNA isolation, PBMC isolation, and CYP450 testing. Most recently, I worked as generalist with emphasis on immunological assays using the BioRad EVOLIS.
Education: B.S., Medical Technology
Towson University
Baltimore, Maryland
Professional Experience:
April 2010 – September 2011 Medical Technologist – Generalist
Wellstar Windy Hill Hospital
Marietta, Georgia
Sole technologist on evening shift and responsible for performing sample and QC testing, troubleshooting any instrument/assay problems, calling critical values, entering and/or reviewing patient results in the LIS. Instruments used include Beckman-Coulter DXC chemistry analyzer, Bayer Clinitek urinalysis analyzer, IL ACL Elite coagulation instrument, Beckman-Coulter LH500 hematology analyzer, Siemens Centaur, and BioRad EVOLIS. Also responsible for performing manual HITAB immunological assay, Hemocult testing, CDIFF testing, positive blood culture gram stains, and I-STAT troponin testing. Computer programs used include SunQuest, Misys, Microsoft Word & Excel. Other duties include logging in all samples, preparing samples for transport to reference labs, checking pending lists, reviewing and updating SOPs.
June 1996 – August 2009 Flow Cytometry Technologist
PCR Technologist
PCR Senior Technologist
Molecular Department Supervisor
Associate Manager, Molecular Department
Manager, Molecular Department
Quintiles Laboratories Limited
Marietta, Georgia
Member of Flow Cytometry Department operating Beckman-Coulter Flow Cytometers and personally developed the use of antibody cocktails to improve efficiency. Worked to set up IL ACL Coagulation instruments and SOPs.
Participated in training by Roche Technical Specialists to perform viral load PCR analysis. Instrumentation used included PerkinElmer 9600 Thermal Cyclers, Microwell plate washer, and Microwell plate reader. Responsible for reviewing all PCR results and QC.
Developed manual assay for genomic DNA isolation, worked with lab management to expand the PCR department by adding Roche COBAS Amplicor instruments. Wrote the SOPs for all assays used in the Molecular Department and trained all staff members. Developed all QC processes and maintenance procedures/forms.
Worked with external customers to set up PBMC isolation procedures, performed and trained staff members on manual sample prep for HCV, HBV, and HIV-1, set up automated genomic DNA isolation using the Gentra Autopure.
Assisted Lab Management with move to new facility. Instrumentation added to the Molecular Department included Roche AmpliPrep, Roche COBAS TaqMan, Luminex 100 with X-map software, Autoblot 3000H, Qiagen Autopure LS, as well as routine lab equipment.
Managed a department of 4 technologists and 1 lab aide, reviewed assay results, QC, instrument maintenance records, SOPs, worked with Quintiles labs in Edinburgh and Hong Kong to develop new assays within the Molecular Test menu, managed department supplies/inventory, responsible for employee performance evaluations. Assays included quantitative measurements of HIV-1 RNA, HCV RNA, HBV DNA, qualitative HCV RNA, HBV and HCV genotyping, genomic DNA extraction, RNA extraction for archival, PBMC isolation, and CYP450 analysis.
November 1992 – June 1996 Supervisor, Immunology Department
Scott & White Memorial Hospital
Temple, Texas
Supervised a staff of 12 people and responsible for the overall management of the department including QC review, results review, and updating procedural manuals. Monitored the use and maintenance of instrumentation that included a Becton-Dickinson FACScan, FACSort, Abbott IMx, and Hybritech Photo-ERA Analyzer. Presented Immunology and Flow Cytometry lectures to the medical laboratory technician class at the local community college.
January 1989 – November 1992 Supervisor, Flow Cytometry Department
National Reference Laboratory
Nashville, Tennessee
Supervised a staff of 10 people and monitored instrumentation that included 6 flow cytometers (Coulter Profile Flow Cytometers, Coulter EPICS 751 Flow Cytometer, Coulter EPICS Elite Flow Cytometer, Ortho Flow Cytometer), 3 hematology analyzers (Coulter JT, Technicon H-1), and routine lab equipment. Responsible for setting up the department, ordering equipment, developing procedures and quality control policies, training employees new to the field of flow cytometry, and reviewing all results, QC, and maintenance logs. Sole author of Focus Article “Basics of Flow Cytometry” in the Jan/Feb 1992 edition of Clinical Laboratory Science magazine.
March 1987 – December 1988 Flow Cytometry Technologist
Center for Clinical Sciences
Nashville, Tennessee
Performed DNA cell cycle analysis, two-color analysis of basic lymphocyte antigens, platelet antibody analysis, leukemia/lymphoma phenotyping, and HLA-B27 typing using the Coulter EPICS V flow cytometer, Coulter Profile flow cytometer, Ortho Cytofluorograf, and the Ortho ELT 15 Hematology Analyzer.
November 1976 – February 1987 Medical Technologist
Oncology Hematology Center
Johns Hopkins Hospital
Baltimore, Maryland
Performed manual WBC and platelet counts, assisted with bone marrows by preparing and staining smears, manually recorded patient sample results in lab log books. Organized the flow cytometry laboratory in a clinical oncology/hematology setting by developing QC procedures as well as clinical test procedures, and trained coworkers in the operation of the Ortho Spectrum III flow cytometer and in sample preparation. Researched and was first author on paper describing flow cytometric differentials, published in the American Journal of Hematology.
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