Researcher
Resume:
Jeffrey R. Anderson
Palos Hills, IL 60465-2676
****@***.***
EXPERIENCE
**/** - *******, ****. **. Research Specialist, Medicinal Chem. & Pharmacognosy, Univ. of Illinois, Chicago, Illinois
• Maintain notebook and data archives, follow safety regulations, train staff, and optimize protocols.
• Organize and analyze data using Excel, GraphPad Prism, GeneMapper, and Sequencher on Mac and PC.
• Operate luminometers, UV spectrophotometers, centrifuges, gel counter, and PCR machines.
• Culture various human cell lines and maintain aseptic tissue culture hoods and incubators.
• Authenticate human and rodent cell lines using species-specific PCR and STR genotyping.
• Optimize and perform manipulative cell culture experiments for RT-qPCR and RE-luciferase assays.
• Optimize PCR and DNA sequencing protocols for species identification of UIC Botanical Center plants.
• Perform PCR, RT-qPCR, western blotting, RE digests, BCA assay, maxiprep, DNA sequencing, cell culture,
transfection, RNA isolation, cDNA synthesis, luciferase assays, cytometry, and transformation.
09/05 - 06/10, Senior Research Technologist, Medicine-Gastroenterology, University of Chicago, Chicago, Illinois
• Maintained notebook and data archives, followed safety regulations, trained staff, and optimized protocols.
• Analyzed data using Excel, DNASTAR, MapDraw, Sequencher, Sequenom, gPlink, and Haploview.
• Prepared figures and charts, graphs, and tables of data for presentation with PowerPoint at regular meetings.
• Conducted bioinformatics research using NCBI, UCSC, HapMap, 1000-Genomes, and EMBL-EBI websites.
• Optimized 384-well PCR, sequencing, and Sequenom genotyping protocols using robotic liquid handlers.
• Performed PCR, DNA extraction, maxipreps, cell culture, TGC electrophoresis, and SNaPshot genotyping.
• Scanned colon cancer probands for DNA repair gene mutations (Ogg1, Apex1,2, Xrcc1, PolB, Lig3, Pcna).
• Genotyped human vitamin D receptor (Vdr) and base excision repair genes for cancer association.
• Cultured and screened human colorectal carcinoma cells for Nsmce2 (DNA repair gene) knockout constructs.
• Sequenced rRNA genes of murine gut microbes and conducted phylogenetic analysis using Megalign.
• Cloned wildtype Bloom syndrome gene (Blm) and SUMO mutants into lentiviral vectors for stable expression.
• Discovered novel SNPs in African, European, and Ashkenazi Jewish Americans.
04/05 - 09/05, Research Specialist, Great Lakes Water Institute, University of Wisconsin, Milwaukee, Wisconsin
• Tracked resources, maintained records, and operated ABI 3730xl DNA Sequencer and BD FACSCalibur.
• Analyzed data using Excel, Vector NTI, DNASTAR, and Sequencher on Mac and PC.
• Performed RNA isolation, cDNA synthesis, RACE, DD-PCR, cytometry, and DNA cycle sequencing.
• Optimized transgenic zebrafish embryo dissociation protocol and GFP cell sorting with F.A.C.S.
• Identified Gap-43 promotor in Medaka fish using Blast searches, ClustalW2, and Megalign.
02/01 - 12/04, Research Technician II, Human Genetics, HHMI/University of Chicago, Chicago, Illinois
• Served as lab manager: interacted with academic agencies, trained personnel, maintained and operated ABI DNA Sequencers,
maintained tissue culture room, purified Taq and LIF, and provided inventory control.
• Analyzed data using Excel, Vector NTI, DNASTAR, and Sequencher on Mac and PC.
• Conducted research using library and Internet resources (NCBI, UCSC, EMBL-EBI, etc.).
• Sequenced genetic orthologs of various mammals (e.g. GenBank Accessions: HM622069-72, HM630302-15, HM771309-27, HM803967-93, HQ141112-
23, HQ437692-HQ437702, AY570943-45, AY570949, AY665212-27, AY485418-24, AY489022, AY488994, AY488966, AY488938, AY488910).
• Conducted high-throughput DNA sequencing of Coriell Human Variation Panel for polymorphism.
• Performed positional cloning (LD genotyping) and gene-trapping (cloning, cell culture, transfections, etc.).
• Maintained mouse embryonic stem cells and human 293 cells to screen for gene trap insertions.
• Maintained primate fibroblast cultures and isolated, immortalized, and cultured primate lymphocytes.
• Performed PCR, DNA extraction, total/mRNA isolation, cDNA synthesis, R.A.C.E, PCR product and sequence purification,
recombinant protein expression and purification (E.coli), and electrophoresis.
EDUCATION
May 2000, Master of Science - Biology (GPA 3.6/4.0), Central Michigan University, Mount Pleasant, Michigan
August 1994, Bachelor of Science - Biology, Benedictine University, Lisle, Illinois
ASSISTED PUBLICATIONS
Genetic associations in the vitamin D receptor and colorectal cancer in African Americans and Caucasians. Kupfer S.S., Anderson J.R., Ludvik A.E., Hooker S., Skol A., Keku T.O., Sandler R.S., Kittles R.A., Ruiz-Ponte C., Castellvi-Bel S., Castells A., Carracedo A., Ellis N.A. PLoS One 2011, 6:e26123.
Genetic heterogeneity in colorectal cancer associations between African and European Americans. Kupfer S.S., Anderson J.R., Hooker S., Skol A., Kittles R.A., Keku T.O., Sandler R.S., Ellis N.A. Gastroenterology 2010, 139:1677-85.
Novel single nucleotide polymorphism associations with colorectal cancer on chromosome 8q24 in African and European Americans. Kupfer S.S., Benn Torres J., Hooker S., Anderson J.R., Ellis N.A., Kittles R.A. Carcinogenesis 2009, 8:1353-57.
3.6kb genomic sequence from Takifugu capable of promoting axon growth-associated gene expression in developing and regenerating zebrafish. Udvadia A.J. Gene Expr. Patterns 2008, 8:382-88. (Acknowledgments)
Sonic Hedgehog, a key development gene, experienced intensified molecular evolution in primates. Dorus S., Anderson J.R., Vallender E.J., Gilbert S.L., Li W., Lahn B.T. Human Molecular Genetics 2006, 15:2031-37.
Trak1 mutation disrupts GABA(A) receptor homeostasis in hypertonic mice. Gilbert S.L., Zhang L., Forster M.L., Anderson J.R., Iwase T., Soliven B., Donahue L.R., Wollmann R.L., Lahn B.T. Nature Genetics 2006, 38:245-50.
Ongoing adaptive evolution of ASPM, a brain size determinant in Homo sapiens. Mekel-Bobrov N., Gilbert S.L., Evans P.D., Vallender E.J., Anderson J.R., Hudson R.R., Tishkoff S.A., Lahn B.T. Science 2005, 309:1720-22.
Microcephalin, a gene regulating brain size, continues to evolve adaptively in humans. Evans P.D., Gilbert S.L., Mekel-Bobrov N., Vallender E.J., Anderson J.R., Vaez-Azizi L.M., Tishkoff S.A., Hudson R.R., Lahn B.T. Science 2005, 309:1717-20.
Accelerated evolution of nervous system genes in the origin of Homo sapiens. Dorus S., Vallender E.J., Evans P.D., Anderson J.R., Gilbert S.L., Mahowald M., Wyckoff G.J., Lahn B.T. Cell 2004, 119:1027-40.
Reconstructing the evolutionary history of microcephalin, a gene controlling human brain size. Evans P.D., Anderson J.R., Vallender E.J., Choi S.S., Lahn B.T. Human Molecular Genetics 2004, 13:1139-45.
Adaptive evolution of ASPM, a major determinant of cerebral cortical size in humans. Evans P.D., Anderson J.R., Vallender E.J., Gilbert S.L., Malcom C.M., Dorus S., Lahn B.T. Human Molecular Genetics 2004, 12:419-21.
REFERENCES
1. Birgit Dietz, Ph.D. (Current Supervisor)
Department of Medicinal Chemistry and Pharmacognosy
University of Illinois at Chicago
833 S. Wood Street (M/C 781)
Chicago, IL 60612
(312) 996 - 2358 (office)
*******@***.***
2. Nathan A. Ellis, Ph.D. (Former Supervisor)
Institute of Human Genetics
University of Illinois at Chicago
900 S. Ashland Ave., MBRB 3352
Chicago, Illinois 60607
(312) 413 - 8616 (office)
(312) 996 - 0626 (fax)
*******@***.***
3. Daniel E. Wujek, Ph.D.-Emeritus (Former Supervisor)
Department of Biology
Central Michigan University
Brooks Hall 164
Mt. Pleasant, MI 48859
(989) 774 - 3626 (office)
(989) 774 - 3462 (fax)
(989) 774 - 3227 (Department)
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