CV

CURRICULUM VITAE

Dr. Kuldeep Kumar

S/o Shri S.R.Shivalya

SHIVALYA SADAN

Ward No.9 Adarsh Nagar Colony

Bazpur (Udham Singh Nagar) Uttarakhand

(M)-+91-971*******, +91-972*******

E-mail- *********@*****.***

http://www.kshivalya.webs.com/

Present Status

Working as Research Associate in NAIP (World Bank Funded) project entitled “Developmental Potency of Parthenogenetic goat embryos” under supervision of Dr. Sadhan Bag, Senior Scientist, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly (UP).

Education

Ph.D. in Biotechnology “PCR based detection of zoonotic Salmonella from foods” from H.N.B. Garhwal University, Srinagar Garhwal under supervision of Prof. Asha Chandola-Saklani, Co-ordinator, Biotechnology Department, H.N.B. Garhwal University, Srinagar Garhwal and co-supervision of Dr. R.K.Agarwal, Principal scientist, Incharge National Salmonella Centre (Vet.) I.V.R.I., Izatnagar Bareilly U.P. Submitted Dec 2009.

M.Sc. in Biotechnology July2006

Kumaun University Nainital, India

Dissertation Title: Antibiotic resistance pattern of Salmonella Virchow.

BSc, Zoology, Botany, Chemistry July 2004

Kumaun University Nainital India

Research Experience

THREE Years research experience working as Research Associate.

Technical Skills

Molecular Biology Techniques

• DNA, RNA Isolation, PCR, RT-PCR.

• Cloning of genes and prokaryotic protein expression studies.

Bioinformatics

• Gen bank BLAST searching, Sequence Alignments, Primer designing.

Microbiology techniques

• Isolation and Characterization of bacteria using conventional, biochemical and serological methods, revival of cultures, storage and maintenance for repository.

Protein Biochemistry studies

• Characterization of protein by one-dimensional SDS-PAGE and Western blot assay.

Mammalian cell culture/ stem cell biology

• Culture and maintenance of mammalian cell line including ES cells.

• Isolation and culture & maturation of Oocytes from buffalo & goat ovaries collected from slaughter house.

• IVF and in vitro culture of Embryos of different developmental stages for isolation & culture of ES cells.

• Microscopy (fluorescent, confocal).

Immunological techniques

• Immunological characterization recombinant protein by ELISA.

Handling of laboratory animal

• Maintenance, handling and treatment of laboratory animals such as Mice, Rat, Rabbit, goat and Poultry birds.

Computer Skills

Proficiency with MS Excel, MS Power Point, MS Word.

Academic Achievements/Awards

• Received Rajiv Gandhi National JRF Fellowship for the year 2007-08 from University Grants Commission New Delhi.

Project/ Training

Project:

• Three month project work on"Antibiotic resistance pattern of Salmonella Virchow" isolates in National Salmonella Centre (Vet.) from Indian Veterinary Research Institute, Izatnagar Bareilly (U.P.). Under supervision and guidance of Dr. R.K. Agarwal, Senior Scientist, Incharge National Salmonella Centre (Vet.).

Training:

• Two month Summer Training in Enzymology Laboratory of Biochemistry Divison of Indian Veterinary Research Institute, Izatnagar, Bareilly, (U.P.) Under guidance of Dr.(Mrs.) Meena Kataria, Senior Scientist.

• Summer school on “Recent Trends in Biology and Biotechnology” organized by the Forest Research Institute, Dehradun and sponsored by the Stare Biotechnology Programme, government of Uttarakhand from 18th to 29th August 2008.

• A training program entitled “Bioinformatics as a tool for gene amplification, cloning and molecular modeling” organized by Animal Biotechnology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly (U.P.) and held under the project of Niche Area of Excellence on Production and Qualoty Control of Veterinary Biologicals from 8th to 13th Decemver 2008.

Professional Affiliation

Member of Society of United Life Sciences India.

Member of World Poultry Science Association, India

National Conferences/Seminars/Symposiums Participated

Regional seminar on “Intellectual Property Right and Innovation Management in Knowledge Era” Organised by National Research Development Corporation, New Delhi and Uttarakhand State Councle of Science and Technology, held in Almora (Uttarakhand) from 28th to 29th Jan. 2008.

6th annual conference of IAVPHS and national symposium on”Horizons of Veterinary Public Health in Augmenting Veterinary, Medical and Environmental health” organised by Department of VPH, Anand Agricultural university, Anand Gujarat from November 29th- 30th, 2007.

National workshop on “Patenting; What, Why, & How” at Department of Biotechnology Kumaun University, Nainital. Sponsored by PFC-TIFAC, DST, Govt. of India on 10 March, 2006.

National Seminar on “Quality assurance and prevention of contamination, adulterants & pollutants in livestock products” Organised by department of Veterinary Public Health, G.B.Pant University of Agriculture & Technology Pantnagar (Uttaranchal), from 27th to 28th February, 2006.

National Conference on advancing frontiers in Biotechnology for Substainable Agriculture & Health organised by the Society for Plant Research, department of Biotechnology Bareilly College, Bareilly from April 28-29, 2005.

73rd Annual Meeting of Society of Biological Chemists (India), Nov.21-24, 2004. G. B. Pant University of Agriculture & Technology, Pantnagar (Uttaranchal).

International Conferences/Seminars/Symposiums Participated

• International Symposium on “Food Safety, Quality, Assurance and Global Trade : Concerns & Strategies” and VIIth annual Conference of Indian Association of Veterinary Public Health Specialist organized by Dptt. of Veterinary Public Health, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology Pantnagar in Collaboration with Michigan State University, USA from 7th Nov. to 9th Nov. 2008.

• International Conference on “Physiological Capacity Building in Livestock Under Changing Climate Scenario” and Silver Jubilee of SAPI (Society of Animal Physiologists of India) organized by Physiology and Climatology Division, Indian Veterinary research Institute, Izatnagar, Bareilly (UP) from November 11th – 13th, 2010.

• International Conference of “9th Asia Peific Poultry Conference” organised by World Poultry Science Association in Taipei, Taiwan from 20th -24th March 2011.

Publications

• K. Kumar, R.K.Agarwal, A.C.Saklani, Shweta Singh, Prejit, M.Singh and K.N. Bhilegaonker (2008). “Antibiotic Resistance Pattern of Salmonella Virchow obtained from animal sources in India”. J. Vet. Pub. Hlth., 6(2): 73-80.

• S. Singh, R.K. Agarwal, S.C. Tiwari and K. Kumar (2009). “Evaluation of a PCR assay for the detection of Salmonella from human clinical samples”, Indian Journal of Comparative Microbiology Immunology and Infectious Diseases 30 (1).20-22.

• V. Gupta, S.K.Shukla, S.K.Shrivastava, S.Shukla, K.Kumar, D.P.Saxena, B.Shrivastava and M.Chaudhary(2010). “Studies of In -Vitro Evaluation and Formulation of Aceclofenac Loaded PLGA Microspheres”. Inter Journal Pharmacology. 6 (5): 726-731.

• S. Singh, R.K. Agarwal, S.C. Tiwari, K. Kumar and P. Nambier (2010). “Predominance of Paratyphi B var Java serotype among human and animal clinical isolates of Salmonella in North India”. J. Vet. Pub. Hlth., 8(2): 73-81.

• Prejit, R.K.Agarwal, K. Porteen, K. Kumar, Shweta Singh, sangeeta Singh and Asha K (2010). “Cloning and sequencing of outer membrane protein C (ompC) gene from Salmonella Typhimurium. J. Vet. Pub. Hlth., 8(2): 111-115.

• D.P.Saxena, S.K.Shukla, K.Kumar, R.Saxena, S.Saxena, S.Shukla, V.Gupta, R. Stephen, H. Kumar, L.Kumar (2011) “Efficacy Studies of In-Vitro Screening of Antiplasmodial Activity by

Crude Extracts of Diospyros melanoxylon”. Research Journal of Medicinal Plants. 5 (3): 312-320.

• K. Kumar, Asha C. Saklani, R.K.Agarwal and Shweta Singh.( 2011) Isolation, plasmid profiling and antibiogram of Salmonella from poultry meat and environmental sources in India. “9th APPC”, Taiwan.

• Satisha K B, Pankaj R S, Venkatesh V, Rohita Gupta, K. Kumar, B C Das, A C Majumdar and Sadhan Bag. “Effect of different activation protocol on generation of parthenogenetic embryos and comparative expression of oct4 and nanog between parthenogenetic and ivf derived embryos in buffalo”. J.Animal Reprod. Sci.(Comm.)

• Pankaj R S, Satisha K B, Venkatesh V, Rohita Gupta, K. Kumar, B C Das , A C Majumdar and Sadhan Bag. “Comparative expression of oct 4 gene in parthenogenetic and ivf derived embryos in goat”. Indian J Ani. Sci. (comm.)

• Pankaj R S, Satisha K B, Venkatesh V, Rohita Gupta, K. Kumar, B C Das , A C Majumdar and Sadhan Bag. “Effect of different activation protocol on generation of parthenogenetic embryos in caprine”. Indian J Ani. Sci. (comm.)

• K Kumar, Renu Singh, R Ranjan, Yasotha T, R K Singh, Manish Kumar, S K Bhanja, B C Das, N H Mohan and Sadhan Bag. “Effecet of “extract egg” in development of parthenonogenetic caprine embryo” Indian J Ani. Sci. (comm.)

• Renu, K Kumar, Iram Khan, R Ranjan, Yasotha T, R K Singh, B C Das and Sadhan Bag. “Comparative expression analysis of embryonic developmental related genes in different stages of haploid and diploid parthenogenetic embryos in caprine” Indian J Ani. Sci. (comm.)

• Gopal Puri, Renu, K Kumar, Iram Khan, Rakesh Kumar, Yasotha T, R Ranjan, B C Das and Sadhan Bag. “Effect of certain cytokines on formation of embryonic stem cell like cells from preimplantation embryos and their characterization in buffalo” J of stem cell & Regenerative Medicine (Comm.)

Sequence Submitted

• GenBank: GQ305124.1, Prejit,N., Agarwal,R.K., Bhilegaonkar,K., Biswas,R., Bagath,M. and Kumar,K Salmonella enterica subsp. enterica serovar Typhimurium strain E-2375 outer membrane protein C protein-like protein precursor (ompC) gene, complete cds

Articles Presented at National Symposia & Conferences

• Rajveer Singh, P.P.Singh, R.S.Rathore, Kuldeep Kumar, Geeta Chauhan, K.Dhama and O.P.Pandey. Studies on river water samples collected from western Uttar Pradesh for detection of Thermophilic campylobacter spp. 12th Indian Agricultural scientists and Farmers congress, 20-21 Feb, 2010 Allahabad UP.

• Shweta Singh, Kuldeep Kumar, R. K. Agarwal and L. Singh (2008). Salmonellosis in Human and animal. XIV annual Convention of Indian Society of Veterinary Immunology and Biotechnology (ISVIB) & national Symposium on “Recent Trends in Immuno-Biotechnology Based Biologicals and Their Commercialization”. Feb. 27-29, Department of Veterinary Biochemistry, G.B.Pant University of Agriculture & Technology, Pantnagar, U.S.Nagar (Uttarakhand).

• Kuldeep Kumar, R. K. Agarwal, M.Singh, M.Z. Siddiqui and S.K. Srivastava (2007). Antibiotic resistance pattern of Salmonella Virchow. 6th Annual Conference of Indian Association of Veterinary Public Health Specialists, and National Symposium on “Horizons of Veterinary Public Health in Augmenting Veterinary, Medical and Environmental Health”. Nov 29-30, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand (Gujarat).

M.Singh, R. K. Agarwal, Kuldeep Kumar, M.Z. Siddiqui and S.K. Srivastava (2007). Antibiotic resistance pattern of Salmonella Typhimurium. 6th Annual Conference of Indian Association of Veterinary Public Health Specialists, and National Symposium on “Horizons of Veterinary Public Health in Augmenting Veterinary, Medical and Environmental Health”. Nov 29-30, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand (Gujarat).

• K. Porteen, R. K. Agarwal, Kuldeep Kumar, and K.N. Bhilegaonkar (2007). Detection of the outer membrane protein C (OmpC) gene in Salmonella serovars of zoonotic significance by polymerase chain reaction. 6th Annual Conference of Indian Association of Veterinary Public Health Specialists, and National Symposium on “Horizons of Veterinary Public Health in Augmenting Veterinary, Medical and Environmental Health”. Nov 29-30, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand (Gujarat).

Articles Presented at International Symposia & Conferences

• Kuldeep Kumar, R.K. Agarwal, A.C. Saklaini, Shweta Singh, Prejit, Sangeeta Singh and V.P.Singh (2008). Evaluation of specificity for invA gene PCR for detection of Salmonella spp. International Symposium on “Food Safety, Quality, Assurance and Global Trade : Concerns & Strategies” and VIIth annual Conference of Indian Association of Veterinary Public Health Specialist organized by Department of Veterinary Public Health, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology Pantnagar in Collaboration with Michigan State University, USA from 7th Nov. to 9th Nov. 2008.

• Kuldeep Kumar, R.K. Agarwal, A.C. Saklaini, Shweta Singh, Prejit, Sangeeta Singh, K.N Bhilegaonkar and V.P. Singh (2008). PCR based detection of Salmonella from chicken meat and poultry faecal samples. International Symposium on “Food Safety, Quality, Assurance and Global Trade : Concerns & Strategies” and VIIth annual Conference of Indian Association of Veterinary Public Health Specialist organized by Dptt. of Veterinary Public Health, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology Pantnagar in Collaboration with Michigan State University, USA from 7th Nov. to 9th Nov. 2008.

• Lakshyaveer Singh, Kuldeep Kumar, J.Kumar, B.bhusan, A.Sharma, S.Singh and A.K.Srivastava (2008). Identification of Gonadotropin releasing Hormone Receptor in different tissuses of pig. International Symposium on “Food Safety, Quality, Assurance and Global Trade : Concerns & Strategies” and VIIth annual Conference of Indian Association of Veterinary Public Health Specialist organized by Department of Veterinary Public Health, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology Pantnagar in Collaboration with Michigan State University, USA from 7th Nov. to 9th Nov. 2008.

• Shweta Singh, R.K.Agarwal, Kuldeep Kumar, R.K.Saxena, Prejit, S.Singh and V.P.Singh (2008). Detection of Salmonella from clinical cases of Human and Animals by PCR and cultural isolation methods. International Symposium on “Food Safety, Quality, Assurance and Global Trade : Concerns & Strategies” and VIIth annual Conference of Indian Association of Veterinary Public Health Specialist organized by Department of Veterinary Public Health, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology Pantnagar in Collaboration with Michigan State University, USA from 7th Nov. to 9th Nov. 2008.

• R.S.Pankaj, Sadhan Bag, B.C.Das, K.B.Satisha, V.Venkatesh, Kuldeep Kumar, Rohita Gupta and A.C Majumdar. Comprative expression of oct4 gene in Parthenogenetic and IVF derived embryos in goat. International conference on reproductive health and 20th annual meeting of the ISSRE organized by deptt of Zoology, University of Rajasthan, Jaipur from 8th Feb to 10th Feb 2010.

• K.B.Satisha, Sadhan Bag, B.C.Das, R.S.Pankaj, V.Venkatesh, N.C.Nikhil, C.Anees, Rohita Gupta, Anand Pandey, Kuldeep Kumar and A.C Majumdar. Comprative expression of oct4 gene in Parthenogenetic and IVF derived embryos in goat. International conference on reproductive health and 20th annual meeting of the ISSRE organized by deptt of Zoology, University of Rajasthan, Jaipur from 8th Feb to 10th Feb 2010.

• Sadhan Bag, Kuldeep Kumar, B C Das and A C. Majumdar. Chronology of oocytes maturation in indigenous caprines. International Conference on Physiological Capacity Building in Livestock Under Changing Climate Scenario and Silver Jubilee of SAPI, IVRI, Bareilly (UP) from November 11th – 13th, 2010.

• Sadhan Bag, Rajni Chhetri, Kuldeep Kumar, B C Das and A C Majumdar. Culture of murine embryonic stem cell on buffalo and goat fibroblast feeder cells. International Conference on Physiological Capacity Building in Livestock Under Changing Climate Scenario and Silver Jubilee of SAPI, IVRI, Bareilly (UP) from November 11th – 13th, 2010.

• K.K Meena., Kuldeep Kumar, G. Gupta, D.K.Singh, R.K. Agarwal and Shrivastav D. Prevalence of Listeria in fruits and fruit juices. International Conference on Physiological Capacity Building in Livestock Under Changing Climate Scenario and Silver Jubilee of SAPI, IVRI, Bareilly (UP) from November 11th – 13th, 2010.

• G. Gupta, Kuldeep Kumar, K. K Meena, D.K Singh, S.K. Shukla and R. K. Agarwal. Studies on prevalence of Escherichi coli and Salmonella in fruits and fruit juices. International Conference on Physiological Capacity Building in Livestock Under Changing Climate Scenario and Silver Jubilee of SAPI, IVRI, Bareilly (UP) from November 11th – 13th, 2010.

• K.K Meena., Kuldeep Kumar, G. Gupta, D.K.Singh, D.P.,Saxena, V. Gupta and K.N. Bhilegoankar Prevalence of Aeromonas in fruits and fruit juices. International Conference on Physiological Capacity Building in Livestock Under Changing Climate Scenario and Silver Jubilee of SAPI, IVRI, Bareilly (UP) from November 11th – 13th, 2010.

• Kuldeep Kumar, Asha C. Saklani, R.K.Agarwal and Shweta Singh. Isolation, plasmid profiling and antibiogram of Salmonella from poultry meat and environmental sources in India. International Conference of “9th Asia Peific Poultry Conference” organised by World Poultry Science Association in Taipei, Taiwan from 20th -24th March 2011.

Paper presented at Short course

Three papers and pricticals in “Recent advances in stem cell research” organized by centre of advanced faculty training in veterinary physiology P & C Division, IVRI, Izatnagar, Bareilly (UP).

1. Sadhan Bag, BC Das, C Anees, Kuldeep Kumar, Rajni Chettri and AC Majumdar. Chromosomal analysis of murine ES cell.

2. Sadhan Bag, BC Das, C Anees, Kuldeep Kumar, Rajni Chettri and AC Majumdar. Immunocytochemical detection of Oct4/Nanog in ES cells.

3. Sadhan Bag, BC Das, C Anees Kuldeep Kumar, Rajni Chettri and AC Majumdar. Molecular characterization of stem cell.

References

Prof Asha Chandola-Saklani, FASc

Co-Ordinator, DST/DBT Projects

Deptt. Of Zoology and Biotechnology

HNB Garhwal Central University

Srinagar Garhwal Uttarakhand 246174

Phone No: 013**-****** (O), 013**-****** (R)

E-mail: ************@*****.***

Dr. R.K. Agarwal, Ph.D.,

Principal Scientist & Incharge

National Salmonella centre (NSCVET)

Division of Bacteriology & Mycology

Indian Veterinary Research Institute

Izatnagar (Bareilly) 243122, U.P.

Mobile No: 91-952*******, 058*-******* (R)

E-mail: *********@*****.***

Dr.K.N.Bhilegaonkar, Ph.D.,

Principal Scientist,

Division of Veterinary Public Health,

Indian Veterinary Research Institute,

Izatnagar (Bareilly) 243122, U.P.

Mobile No: 91-941*******, 058*-******* (R)

E-mail: ********@**********.***

Personal Introduction

Name Kuldeep Kumar

Father’s Name Shri S.R. Shivalya

Mother’s Name Smt. Mithlesh Shivalya

Date of birth 22 Jan, 1982

Gender Male

Marital Status Married

Nationality Indian

Language Proficient in English & Hindi

Hobbies Participation in scientific activity, reading Research paper, Internet.

Declaration

I hereby declare that the above information given by me are true the best of my knowledge and belief.

Date:

Place: (KULDEEP KUMAR)

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