Manufacturing Maintenance

Resume of Fernando Arias

EDUCATION:

University of Santiago Chile Santiago, Chile

Bachelors in Biology

Harvard extension school Boston, MA

ESL course 2005

EXPERIENCE:

Xcellerex

Manufacturing Associate II January 2009 - November 2010

Responsible for maintaining all types of cell culture responsibilities including calculating daily feed, expansion, and cell counting

Responsible for following batch records and recording data according to GMP guidelines

Cell culture including human, animal and insect cell lines ( sf9, cho. per.c6, hi5)

Responsible for assembling the bio-reactor, and then processing and harvesting the cells

Worked with new cell culture technology, the Disposable Bioreactor

Completed whole process from thaw, maintenance, cell culture, expansion, harvest, and purification in GMP setting

BD Biosciences

Manufacturing Associate II December 2007-2008

Responsible for following batch records and recording all data according to GMP guidelines

Head of the 150 donor processing team (human livers).

Complete cell line maintenance: Expansion, Feeding, Gassing.

Worked with Hi5 cell line and S9 cell line

Human fresh hepatocytes

Human and primate microsome, S9 and cytosol extraction

Blending Batches

Lowry protein assay procedure

Cell counting

Experience with the plating process

Cytochrome P450 spectral quantization

Biopsy processing

AMAG Pharmaceutical February 2005- October2007

Acquired knowledge: Vial washing, Depyrogenation, Solution preparation, Reactor process, filtration and Fill finish

Genzyme Biosurgery Cambridge, MA

Senior Manufacturing Technician January 2000- December 2004

Support Epicel by training new patient coordinators.

Trained support technicians in all procedures and increased productivity by improving processes.

Epicel cultured keratinocytes for burned patients:

Mastered the cell culture techniques, including processing of biopsy cells, maintenance, expansion and grafting

Manufacturing Assistant

Acquired knowledge of GMP.

Documentation and traceability of records.

Trained all aspects of glassware room, R/O water systems, autoclave, and dry heat oven.

Animal surgery procedures

Use aseptic techniques in preparing media and in sterilizing materials.

Order materials and media for the manufacturing department.

Trained using MFG software.

Human tissue cell extractions

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