Process development scientist

Annathur Kalingan.

*** ********** ****, *** #****

Houston

TX 77058.

*********@*****.***

Cell Phone: 832-***-****

EDUCATION

MHA Healthcare Administration. May 2009 University of Houston, Clear Lake, Texas.

Ph.D. Microbiology Sept 1996, Anna University, Chennai, India.

RESEARCH EXPERIENCE

University of Oklahoma Health Sciences Center, Oklahoma City, OK.

Postdoctoral fellow ( with Dr.Wen Wu), 1st March 2004 to 31st July.

Thorough knowledge on Radioimmunoassay and ELISA. Experimental techniques involve routine isolation of mRNA, RNA, & DNA, and other related molecular biology work.

Oklahoma State University, School Of Chemical Engineering, Stillwater, OK.

Postdoctoral fellow (with Dr.Lewis), 1st October 2002 28th Feb 2004

Explored different low cost carbon & nitrogen sources and conducted kinetic studies on Clostridium carboxidovorans cell growth and product (ethanol & butanol) formation. Immobilization of cells on low cost matrices to increase the ethanol yield has shown some promises. My research work primarily revolved around process optimization, to convert CO / CO2 to ethanol using clostridium carboxidovorans. Presently I am a working on cell recycling systems using New Brunswick fermenter (Bioflo 110) aiming to achieve high cell density.

National Taiwan University, Bioenvironmental Systems Engineering, Taiwan

Postdoctoral fellow (with Dr. Liao) November 2000 July 2002

Evaluated the recovery of riboflavin using spray driers, vacuum driers, freeze-drier and rotavapour. Biodegradation of ammonia using biocatalyst in biofilters. Designed and determined stability of various biofiltration matrices, Analyzed adsorption and degradative properties of packing matrices in combination with microbial population.

Academia Sinica, Institute of Biomedical Sciences, Taiwan

Postdoctoral fellow (with Dr. Steve) April 2000 October 2000

Working knowledge on molecular biology techniques with emphasis on site directed mutagenesis involving primer design, RT-PCR, agarose gel electrophoresis, plasmid purification, restriction digestion, ligation, transformation, DNA sequencing and confirmation of mutants. Also Familiar with isolation of RNA, mRNA from sheep cells. Thorough knowledge on radio immunoassay and ELISA.

Indian Institute of Science, CRBME, Bangalore, India

Post-doctoral fellow (with Dr. Dighe) Apr 1995 - Mar 1996

Cloned and expressed the DNA encoding chicken riboflavin carrier protein (RCP) in methylotrophic Pichia pastoris using pHIL D4 expression vector. The RCP activity was checked employing polyclonal antiserum. It was found that the Pichia sp cells could synthesize and secrete RCP like activity into the medium. The level of RCP expression was approximately 450 microgram/ L of the medium estimated in radioimmunoassay.

WORK EXPERIENCE

Biomed Hitech Industries Limited, Quality Control Department. Madras, India

Manager (Reporting to Chairman) Aug 1996 - July 1998

Examined routine laboratory operations in medical device manufacturing unit. This includes bio-burden assays, screen environmental monitoring plates, assessing growth and product particulate matter, LAL pyrogen testing of raw materials and final products. Sterilization and validation activities as per standard operating procedures (SOP). Quality systems interfacing with regulatory agencies. Writing and reviewing standard operating procedures, batch records, protocols and reports. Trained in Fresenius Medical Care , Germany at St. Wendell.

PUBLICATIONS.

Kalingan, A.E. & Randy S. Lewis. Comparison of growth profile of Clostridium carboxidovorans in batch, continuous and recycled fermentations under the influence of producer gas. (In preparation for Biotechnology progress.)

Kalingan, A.E., Chung-Min Liao. Jein-Wen Chen; Szu-Chieh Chen (2004) Microbial degradation of livestock-generated ammonia by using biofilters at typical ambient temperatures. Journal of Environmental Science and Health (Part B) B39, 1, pg 185-198.

Kalingan, A.E. & Chung-Min Liao. (2002) The influence of type and concentration of the flavinogenic factors in production of riboflavin by Eremothecium ashbyii nrrl 1363. Bioresource Technology Vol.82, 3. pg 219- 224

Kalingan, A.E. (1998) The kinetics of riboflavin secretion by Eremothecium ashbyii nrrl 1363. Bioprocess Engineering Vol. 18, No.6. pg. 445-449.

Kalingan, A.E. & Krishnan, M.R.V. (1997) Agro-industrial by-products as flavinogenic stimulators for riboflavin production. Bioprocess Engineering - Vol. 17, No. 2. pg. 87-91

Kalingan, A.E. & Krishnan, M.R.V. (1997) Application of agro industrial by products for riboflavin production by Eremothecium ashbyii nrrl 1363. Applied Microbiology & Biotechnology - Vol. 47, No.3, pg. 226 - 230.

Kalingan, A.E. & Krishnan, M.R.V. (1996) Fungal fermentation of Agro-industrial waste to produce riboflavin, Journal of Indian Institute of Chemical Engineers Section A, Vol. 38, No. 2, pg. 43 - 47

Kalingan, A.E. & Krishnan, M.R.V. (1995) Riboflavin - A status report, Journal of Indian Institute Chemical Engineers. Section B - Industry and News. Vol. 37, No.1 & 2 pg. 14 - 22.

HONOURS / AWARDS

Post-doctoral fellowship, awarded in OSU-Stillwater, September 2002 February 2004

Post-doctoral fellowship awarded in Taiwan, November 2000 &July 2002

Post-doctoral fellowship awarded in Taiwan, April 2000 & October 2000

Contributions and achievement recognized by internationally respected Marquis Whos Who in the World 2000;, Directory 17th Edition

Post-doctoral fellowship awarded in Indian Institute of Science April 1995-Mar 1996

Awarded second prize while representing & Biotechnology Consortium India Ltd. in green Expo exhibition in January 1992 at Madras, India

REFERENCES

Will be provided upon request.

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