Clinical lab
Resume:
Yong-Feng Jiang
West Roxbury, MA 02132
Tel: 617-***-****
**********@*****.***
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Summary
Specific experience in rodent disease models, cell-biology and immunology includes: the rat model of renal ischemia and cerebral ischemia, pancreatic disease models in rats and rabbits, and mice asthma and allergy model, flow cytometry, immunohistochemistry, Calcium Flux assay, Lenti (retro) virus SiRNA knock down system, mammalian tissue culture, In vitro cell proliferation assays, and HPLC.
Professional Experience
2004-present Brigham & Women's Hosp, Harvard Medical School. Boston, MA
Research Fellow,
• Focus on Immunology, mast cell biology, asthma models.
• Established that cysteinyl leukotrienes (cys-LTs ) trigger proliferation by inducing transactivation of c-Kit receptor tyrosine kinase.
• Established that the cys-LT1 and cys-LY2 receptors function as a heterodimer on mast cells. I have used energy transfer techniques(FLIM-FRET), confocal imaging, and ShRNA knock-down and knock out mice approaches to establish that cys-LT2 receptor function in this heterodimer as a negative regulator of mitogenic signaling.
2000-2003 Brigham & Women's Hosp, Harvard Medical School. Boston, MA
Research Fellow,
• Demonstrated that calcium-sensing receptor (CaR) functionally works as a homodimer, and PKC phosphorylation of the CaR modulates functional interaction of G-proteins with the CaR cytoplasmic tail.
1999-2000 Meharry Medical College, Nashville, TN. Postdoctoral fellow,
• Investigated the protein-protein interaction of sindbis virus by using yeast Two-Hybrid system.
1997-1999 INSERM U315, Unite de Recherches de Physiologie et Pathologie
Digestives. Marseille, France Postdoctoral Fellow
• Cloned a new gene (we named it VMP1) which is expressed in high levels in many organs during the acute phase of acute pancreatitis.
1994-1997 Shanghai Medical University. Shanghai, China. PhD. candidate.
• Established the ideal animal models with rat and rabbit for acute pancreatitis research. Demonstrated the role of cytokines such as IL6, IL-1B and TNF-α in AP.
1993-1994 Affiliated Hospital of Shandong Medical University. Shandong, China
• Assistant professor. Performed regular clinical practice in GI surgery, and participated in teaching of surgical principle and clinical skill for medical students.
1990-1993 Affiliated Hospital of Qingdao Medical College. Qingdao, China
• Performed general clinical practice mainly in GI Surgery. Conducted a study of the expression of estrogen receptor on human colorectal cancer by using immunohistochemical assay.
Education
Bachelor of Medical science, Qingdao Medical College, Qingdao, China
Master of General Surgery, Qingdao Medical College/ Shanghai Medical University.
Honors and Awards
2002 Dual Mentored Fellowship. Brigham and Women's Hospital, Harvard Medical
School. Boston, USA
2005 Training Grant of NIH, Brigham and Women's Hospital, Harvard Medical
School. Boston, USA
2008 Committee of Brigham and Women Hospital’s Confocle core facility.
Publications/Presentations/Poster Sessions
1. Jiang Y, Borrelli L, Bacskai BJ, Kanaoka Y, Boyce JA. P2Y6 receptors require an
intact cysteinyl leukotriene synthetic and signaling system to induce survival
activation of mast cells. The Journal of Immunology. 2009 Jan 15,182 (2):1129-37.
2. Jiang YF, Borrelli L, Kanaoka Y, Bacskai B, and Boyce JA, CysLT2 Receptors
Interact with CysLT1 Receptors and Down-Modulate Cystinyl Leukotriene-
Dependent Mitogenic Responses of Mast Cells. Blood: 2007 Nov, 110(9): 3263-70
3. Jiang YF, Kanaoka Y, Feng C, Karl N, Sudhir R, and Boyce JA, Cutting Edge:
Interleukin 4-Dependent Mast Cell Proliferation Requires Autocrine/Intracrine
Cysteinyl Leukotriene-Induced Signaling. The Journal of Immunology: 2006 Sep,
177(5): 2755-59
4. Jiang YF, Minet E, Zhang Z, Silver PA, Bai M. Modulation of interprotomer
relationships is important for activation of dimeric calcium-sensing receptor, The
Journal of Biol Chem. 2004 April, 279(14): 14147-56
5. Jiang YF, Kifor O, Lane C, Quinn SJ and Bai M, PKC phosphorylation of the CaR
modulates functional interaction of G proteins with the CaR cytoplasmic tail, The
Journal of Biol Chem. 2002 Dec, 277(52) : 50543-49
6. Zhang Z, Jiang YF, Quinn SJ, Krapcho K, Nemeth EF, Bai M, L- phenylalanine and
NPS R-467 synergistically potentiate the function of the extracellular calcium-sensing
receptor through distinct sites, The Journal of Biological Chemistry: 2002 Sep,
277(37): 33736-41
7. Dusetti NJ, Jiang YF, Vaccaro MI, Tomasini R, Azizi Samir A, Calvo EL,
Ropolo A, Fiedler F, Mallo GV, Dagorn JC, Iovanna JL, Cloning and Expression of
the Rat Vacuole Membrane Protein 1 (VMP1), Biochemical and Biophysical
Research Communications, 2002 Jan, 290(2): 641-9
8. Jiang YF, Vaccaro MI, Fredler F, Calvo ZL, Iovanna JL, Lipopolysaccharides induce
p8 mRNA expression in vivo and in vitro, Bioch & Bioph Research Comm, 1999 July.
260(3): 686-90
9. Jiang YF, Yang J, Ciu Z, Zhou Y, He Q, Studies on the estrogen receptor in
human colorectal carcinoma. Chinese J of Experi Surgery, 1996 May, 13(5), 15-18
10. Ni Q, Jiang YF, Analysis of prognostic factors for acute pancreatitis, Acta
Academiae Medicinae Shanghai . 1996 Jan, 23(1): Pages: 256-58
11. Jiang YF, Ni Q, Zhang Y, Exploring ideal model of acute pancreatitis in rats,
Acta Academiae Medicinae Shanghai. 1996 Jan, 23(1): 25-30
Poster
1. Jiang YF and Boyce JA. CysLT2 receptor Negatively Regulate CysLT1 Receptor
Function on Mast Cells Through Heterodimerization. AAAAI Annual Metting. San
Diego, California.. 2/2007 (oral poster presentation)
2. Jiang YF and Boyce JA. CysLT1 Receptors Form Heterodimers with the P2Y6
Pyrimidinergic receptor; a Basis for Cross-Talk and Diversity of Inflammatory
Leukotriene and Nucleotide Signaling. Keystone Symposia, Eicosanoids in
Inflammation and Chronic Diseases. Park City, Utah,, 1/2006
3. Jiang YF and Bai M, Phosphorylation of the CaR Blocks G-proteins from
Functionally Interacting with Cytoplasmic Tail of caR . The XIVth World Congress
of Pharmacology. San Francisco, CA. 7/2002
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