High School Technician

KRISTA LYN HAGLUND

Phone: 314-***-**** Email: ******.*******@*****.***

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SUMMARY

Senior Research Technician most recently performing finishing techniques for the Genome Center at Washington University. Experience performing multiple assays on various species of laboratory animals. Experience with instrumentation such as MJ Research and Perkin Elmer cyclers for DNA sequencing, UV/VIS spectrophotometer, Gould TAII Physiograph Recording System, analytical balances, Hamilton repeating syringes, and various pipettes. Computer software experience with Consed, SAS, Microsoft Word, Microsoft Excel, various email programs and Ponemah Digital Acquisition Analysis and Archive Systems. Extensive experience with animals in both a veterinary and a research setting. Bachelor of Science Degree: Coursework included: Biochemistry, Biology, Genetics, Inorganic Chemistry, Nutrition, Organic Chemistry, Physics, Statistics, Physiology, and Zoology.

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PROFESSIONAL EXPERIENCE

Genome Center / Washington University School of Medicine, St. Louis, MO Oct.2000 – June 2011

Senior Research Technician

Research Technician performing finishing techniques according to Genbank finishing standards.

Responsibilities:

Evaluation and manipulation of Consed databases in order to assure the consensus standards for what is considered to be finished sequence are met in all areas for all genomic databases, which are to be submitted to Genbank. Performance of PCR, dye primer sequencing, and dye terminator sequencing techniques, in order to gather additional data to meet consensus standards. Verification of the accuracy of finished genomic databases using restriction digests and various computer programs, specifically designed to check accuracy prior to submission to Genbank.

Monsanto, St. Louis, MO Feb.2000 - Oct. 2000

Research Biologist

Biologist developing and performing assays for the evaluation of the effects of nutraceuticals on hypertension in animal models.

Responsibilities:

Performance of assay validation for the efficacy of nutraceuticals, monitoring chronically catheterized rats with a physiograph and data acquisition software; the validation required the development of methods and familiarization with the physiograph and associated software. Creation of drafts of SOPs for assay activities. Assistance with metabolism study, preparation of data collection forms, and assistance with in phase specimen collections. Representation of the Nutrition sector on the Monsanto Biosafety Committee and representation of the Nutrition Pre-Clinical team on the Nutrition Health and Safety Committee.

Celsis Laboratory Group, Inc., St. Louis, MO Aug. 1999-Jan. 2000

Biological Analyst (Aug.1999 - Jan. 2000)

Member of the Biology Department analytical team, provided contract laboratory services to clients in pharmaceutical and raw materials industries, conducted studies under GLP and/or GMP regulations.

Responsibilities:

Performance of assays involving various species, including rats, mice, rabbits, guinea pigs, cats and chickens. Performance of Pyrogen tests, Toxicity tests, and General Safety tests, according to the appropriate USP or CFR methods. Underwent training to perform Oxytocin Potency and Depressor tests, using a physiograph to monitor blood pressure. Preparation of client samples for testing. Preparation of media for the Department of Microbiology. Performance of calibration checks on analytical balances.

Monsanto (AgDairy Business), Chesterfield, MO Today’s Staffing (Temp) Dec. 1997 - July 1999

Research Biologist

Biologist performing quality control assays in a GMP/GLP environment, for the commercial release and the research and development of bovine somatotropin. Qualified to perform all aspects of all varieties of bioassays performed by the team. Recognized for contributing to the preparation and accomplishment of performing in-house pharmacokinetic studies, which saved the business over $60,000 in 1998. Also, recognized for contributing to the completion of the 1998 year-end go/no go milestone of the Replacement Heifer team, through data verification.

KRISTA LYN HAGLUND (cont.) Phone: 314-***-**** Email: ******.*******@*****.*** Page 2

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Responsibilities:

Performance of rat growth assays on bovine somatotropin for both commercial release and research, including weight collection and injection of rats, and preparation of treatments. Solubilization of powdered bovine somatotropin, including operation of UV/VIS spectrophotometer. Performance of rat pharmacokinetic assays for bovine somatotropin research and development. Ordering of animals for the Rat Bioassay team, utilizing the CAPS system. Preparation of computer files for assays. Execution of computer programs involved with bioassays, including running statistical analysis using SAS. Receipt of bovine somatotropin samples and logging samples into Newlims computer system. Scheduling of assays for the Rat Bioassay team. Interaction with Monsanto Laboratory Animal Resources personnel relating to GMP documentation procedures. Experience in performance of bovine somatotropin radioimmunoassays. Underwent training on the performance of ELISA tests on the Basic Robotic Immunoassay Operator.

Animal House Veterinary Hospital, Arnold, MO June 1992 - Dec. 1997

(part/full time while attending college)

Veterinary Assistant

Member of staff, providing care to patients and education to customers.

Responsibilities:

Assistance with surgical procedures. Care and handling of dogs, cats and other small animals. Preparation of samples in laboratory for diagnosis and analysis of blood samples on Vet Test equipment. Use of Soft Vet computer program. Training of new employees. Preparation of vaccination schedules for customers, along with education of customers about health care and safety of pets. Assistance with examination and treatment of animals.

Horizon Farms, Inc., Barrington, IL (internship) Jan. 1997 - Apr.1997

Internship

Intern working in the breeding division of a premier Thoroughbred breeding and training facility. Provided daily care of mares, foals and stallions.

Responsibilities:

Assistance with semen collection from stallion and analysis of semen using a UV spectrophotometer. Assistance with artificial insemination of mares and natural breeding. Assistance with foaling and medication of mares and foals. Assistance with veterinary exams and treatments of mares and foals.

EDUCATION

Bachelor of Science-Animal Science; University of Missouri-Columbia, December 1996.

Course work in Biochemistry, Biology, Genetics, Inorganic Chemistry, Nutrition, Organic Chemistry, Physics, Statistics, and Zoology.

Volunteered with the University Raptor Rehabilitation Project, caring for birds of prey.

AutoMax Training: Completed AutoMax sales training course in May 2012.

Honors

Awarded the Wurdack Scholarship by the University of Missouri-Columbia, Department of Agriculture in 1992. Awarded the Mark Woods Memorial Scholarship by Hillsboro High School in 1992. National Honor Society 1989-1992, Who’s Who in America, High School Students, 1992

Coursework/Committees

Several Safety Courses at Monsanto

Served on Several Safety Committees at Monsanto

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