Project Assistant

Jennifer M. Frame

**** **** ***** **. 361-***-****

Irvine, CA 92617 ******@***.***

Job Objective: To obtain a challenging position in a progressive laboratory that will utilize my education and experience to make a meaningful contribution to society through science.

Education:

University of California, Irvine, CA

Master’s of Science in Biotechnology with Stem Cell Emphasis

2006-2008 University of Arizona, Tucson, AZ

Non-degree seeking graduate student; Molecular Neuropharmacology and Molecular and Cellular Neurobiology, Cells to Organs, Systems Physiology

2001-2005 University of Arizona, Tucson, AZ

Bachelor of Science in Molecular and Cellular Biology; Minor in Chemistry

Job Experience:

University of California, Irvine – Keirstead Research Group – RIRC,

Staff Research Associate II, May 2008-July 2010.

Cultured Motor Neurons, Schwann cells, and Myoblasts.

Performed Co-Culture experiments using axon isolation chambers.

Prepared Lenti-viral constructs for transduction of myoblasts and Schwann cells

Designed and executed an In Vivo experiment to determine GDNF secretion with transduced human myoblasts In Vivo.

Designed and executed q-PCR experiments to determine levels of inflammation in a contused rat spinal cord.

Performed ELISA’s and Western Blots to determine concentrations of cell supernatants.

Performed Taqman Protein Assays.

Used the IVIS Lumina Animal Imager to track stem cell migration.

Assists graduate students in rat surgeries and project goals.

Presented a poster at the 13th International Symposium on Neural Regeneration, on Detection of GDNF Secretion in Muscle.

Authorship on a paper in The Journal of Stem Cells called Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Improve Recovery after Cervical Spinal Cord Injury. J.Sharp et al.

Authorship on a paper in Stem Cells International called Human Motor Neuron Progenitor Transplantation Leads to Endogenous Neuronal Sparing in 3 Models of Motor Neuron Loss. T. Wyatt et al.

University of Arizona – Arizona Research Labs – Division of Biotechnology – Human Origins Genotyping Laboratory, Research Specialist, March 2008 –May 2008

Performed high throughput genotyping of mice

Managed undergraduate training as well as assisted other technicians with training

Conduct daily operations necessary for continued progress of samples through testing procedures

Coordinating other laboratory staff to perform group procedures

Coordinating HOGL projects with collaborators

Directing laboratory staff to perform procedures

Managing sets of samples through the appropriate procedures to produce the necessary data sets (including retesting of drop-outs)

Providing guidance or assistance when necessary

Assist with research, development, and facilitation of additional laboratory projects

University of Arizona – Arizona Research Labs – Division of Biotechnology – Human Origins Genotyping Laboratory, Research Technician, July 2005 – March 2008

Performed Genotyping on human samples from Family Tree DNA Inc. and National Geographic’s Genographic project.

Designed PCR experiments and sequencing primers in both mice and human genomes

Maintained polymorphism and primer databases

Assisted with preparation and writing of scientific articles for publication

Provided assistance with undergraduate student research projects

Provided assistance with new hire training and orientation to the lab

Assumed a leadership role on a multiplex STR analysis project

Designed and presented a poster on “Customizing Commercially Available DNA Extraction Kits”, at the 18th Annual Promega Human Identity Conference in Hollywood, CA. in Oct. 2007

University of Arizona - College of Nursing – Biolaboratory Tucson, AZ

Lab Assistant/ Scientist, July 2002 - May 2005

Participated in research investigating phospholipid levels in childhood leukemia and traumatic brain injury

Assisted in the presentation of results of a traumatic brain injury study at the 2004 Neurocritical Care Society meeting in San Diego, CA

Lab Experience:

Allergan Inc. – Irvine, CA, Summer Intern, June 2010-September 2010

Investigated gene expression in age related macular degeneration in rat and human samples

Performed FLIPR assay for detection of calcium release with agonists and antagonist drugs

University of Arizona - College of Medicine - Neurology laboratory Tucson, AZ

Lab Assistant/ Student, January 2005 - May 2005

Injected DNA into spinal canals of chick embryos

Used known protocols to discover gene expression of stem cells in adult mice and in stage 12 chick embryos

Used electroporation techniques to determine gene expression in chick embryos

Extracted cells from adult mouse spinal cords \

Experience with laboratory automation systems.

Experience with data from ABI capillary electrophoresis system.

Skills/Abilities:

Proficient in Microsoft Word, Excel, and Power Point applications

Communicates clearly and concisely with co-workers

Performs job duties in an accurate and timely fashion; adapts to new duties quickly

Able to quickly organize and execute job duties.

Proficient in the Primer3 application and Genotyper software

References:

Available upon request

Contact this candidate