Molecular Biology Ph D

Location:

Indianapolis, IN

Posted:

November 25, 2024

Contact this candidate

Resume:

Chiranjeevi Tikka. Ph.D.

School of Medicine Indiana University

Indianapolis- IN 46202

Contac: +1-317-***-****

********@**.***

*****.*****@*****.***

Professional Summary

I am a dedicated researcher specializing in tumor immunology and T-cell biology, with a strong commitment to advancing knowledge in cancer biology. My research focuses on identifying pivotal molecular and metabolic factors influencing cancer initiation and progression. I bring extensive experience in immunology, molecular biology, and microbiology, with a proven track record of investigating adaptive immune responses in cancer. Currently a post-doctoral researcher at Indiana University's School of Medicine, I study IL-9 producing Th9/Tc9 cells and CD4 CAR T cells to enhance anti-tumor immunity. I excel in experimental design and execution, proficient in techniques such as flow cytometry, ELISA, Western blotting, and molecular biology assays. Skilled in data analysis using statistical software and bioinformatics tools, I have contributed to multiple peer-reviewed publications and presentations at prestigious conferences. My passion lies in applying innovative research methodologies to unravel complex immunological mechanisms in cancer.

Education

• Ph.D. (Biotechnology): 2010 -2014. Sri Venkateswara University, Tirupati, AP-India.

• Master of Science (Biotechnology): 2006-2008. Periyar University, Salam, Tamil Nadu, India.

• Bachelor of Science (Botany, Zoology, Chemistry): 2002-2005. Sri Venkateswara University, Tirupati, AP-India. Research Experience

Post-Doctoral Researcher

School of Medicine, Indiana University, Indianapolis. Sep 2021 – Present

o Investigating adaptive immune responses in cancer, focusing on IL-9 producing Th9/Tc9 cells and the role of CD4 CAR T cells.

o Studying the regulatory role of PTPN2 in T-cell functions. o Explored the function of LKB1 in M2-like tumor-associated macrophages and its impact on bone marrow-derived macrophages (BMDM).

o Investigated BATF’s role in maintaining bone marrow Treg cell homeostasis and its influence on hematopoiesis and B cell development.

o Examined GPX4's involvement in Treg cells, ferroptosis, and lipid metabolism. Research Associate, Sri Venkateswara University, Tirupati, India. Oct 2019 - Aug 2021

o Investigated the role of NOD2 in gut microbiome dynamics and its implications in arsenic-induced colon cancer.

o Explored mTOR signaling pathway in arsenic-induced neurotoxicity across brain regions. Post-Doctoral Researcher, Shanxi Agricultural University, Shanxi, China Mar 2017 - Sep 2019

o Studied the interrelation between NOD2 and gut microbiome in arsenic-induced models. o Investigated autophagy in brain regions affected by arsenic exposure. Teaching Faculty Aug 2015- Feb 2017 (Academic)

Skills

Research Expertise:

Tumor Immunology and T-Cell Biology:

o Investigated adaptive immune responses in cancer. o Studied the T-cell regulation within the tumor microenvironment. Immunotherapy:

o Explored the role of BATF in maintaining bone marrow Treg cell homeostasis and its impact on hematopoiesis and B cell development.

o Investigated GPX4’s influence on Treg cells, ferroptosis, and lipid metabolism. Microbiology and Molecular Biology:

o Researched the role of NOD2 in gut microbiome dynamics and its implications in colon cancer in arsenic-exposed models.

o Examined mTOR signaling in arsenic-induced neurotoxicity across brain regions. Laboratory Techniques:

• Techniques: Flow cytometry, ELISA, Western blotting, qRT-PCR, IsoPlexis, IHC, IF and electroporation.

• Cell culture Techniques: Mammalian cell culture, immune cell isolation from both mouse and human (T- cell enrichment and cloning, CAR-T cell cloning), Co-culture, PBMCs isolation from Buffy coat and maintenance, Microbial cell culture and gene cloning.

• Model Systems: Proficient in using knockout animal models and human samples. Data Analysis and Scientific Tools:

• Analytical Skills: Strong proficiency in statistical software such as GraphPad Prism and Flowjo.

• Bioinformatics: Trained in Next Generation Sequencing (NGS) and data analysis from AU-KBC Research Centre.

Scientific Communication:

• Writing and Presentation: Excellent scientific writing skills, evidenced by multiple publications and conference presentations.

Training Programs

• Next Generation Sequencing (NGS)- Bioinformatics and data analysis training from AU-KBCR Research Centre, Madras Institute of Technology (MIT), Madras, India- 2014.

• Two-week international training on Bio-informatics 4th Jan to 18th Jan 2021. Awards

• Best Research / Idea Award Biomedical Innovations and Small Business Opportunities SVIMS 22-23-01-2020.

• Basic Science Research (BSR): UGC-Delhi, India 2012 to 2014. Books & Chapters

• Book entitled “"Periodontitis and Diabetes-Microbial Pathogen study as marker" (ISBN 978- 3-659-84425-6).

Publications

Google Scholar: https://scholar.google.com/citations?hl=en&user=HANgZ6UAAAAJ 1. Tikka C, Beasley L, Xu C, Yang J, Cooper S, Lechner J, Gutch S, Kaplan MH, Capitano M and Yang K (2023) BATF sustains homeostasis and functionality of bone marrow Treg cells to preserve homeostatic regulation of hematopoiesis and development of B cells. Front. Immunol. 14:1026368. doi: 10.3389/fimmu.2023.1026368.

2. Chiranjeevi Tikka, Ram Kumar Manthari, Ruiyan Niu, Zilong Sun, Jundong Wang. Dendritic cell CX3CR1 and macrophages F4/80 play a central role in betweengut micro biome and inflammation in Arsenic induced mice (2021); BioRxiv preprint doi: https://doi.org/10.1101/2021.01.25.428065. 3. Chiranjeevi Tikka, Ram Kumar Manthari, Mohammad Mehdi Ommati Ruiyan Niu Zilong Sun, Jianhai Zhang, Jundong Wang. Immune Disruption Occurs through Altered Gut Microbiome and NOD2 in Arsenic induced Mice: Correlation with ColonCancer Markers. Chemosphere 246 (2020) 125791 https://doi.org/10.1016/j.chemosphere.2019.125791. 4. Manthari R.K., Tikka, C, Ommati, M.M., Ruiyan Niu., Zilong Sun., Jinming Wang Jianhai Zhang Jundong Wang. Arsenic induced autophagy in developmental mouse cerebral cortex and hippocampus by inhibiting PI3K/Akt/mTOR signaling pathway: involvement of blood- brain barrier’s tight junction proteins, Arch Toxicol, (2018) 92:3255. https://doi.org/10.1007/s00204-018-2304-y 5. Ram Kumar M, Chiranjeevi Tikka, Mohammad Mehdi Ommati, Ruiyan Niu, Zilong Sun, Jinming Wang, Jianhai Zhang, and Jundong Wang. Arsenic-Induced Autophagy in the Developing Mouse Cerebellum: Involvement of the Blood-Brain Barrier’s Tight-Junction Proteins and the PI3K-Akt-mTOR Signaling Pathway, Journal of Agricultural and Food Chemistry, (2018) 8602- 8614.DOI:10.1021/acs.jafc.8b02654. https://doi.org/10.1021/acs.jafc.8b02654. 6. M.M. Ommati R. Heidari, R.K. Manthari, Tikka Chiranjeevi, R. Niu, Z. Sun, S. Sabouri, M.J. Zamiri, L. Zaker, J. Yuan, J. Wang, J. Zhang, J. Wang. Paternal exposure to arsenic resulted in oxidative stress, autophagy, and mitochondrial impairments in the HPG-S axis of pubertal male offspring, Chemosphere, 236 (2019) 124325. https://doi.org/10.1016/j.chemosphere.2019.07.056 7. Mohammad MehdiOmmati, Ram Kumar Manthari, Chiranjeevi Tikka, Ruiyan Niu, Zilong Sun, Samira Sabouria, Mohammad Javad, Zamiri Hasan, Nategh Ahmadi, Hasti Ghaffarie, Reza Heidari, Jundong Wang. Arsenic-induced Autophagic Alterations and Mitochondrial Impairments in HPG-S Axis of Mature Male Mice Offspring (F1-generation): A persistent toxicity study. https://doi.org/10.1016/j.toxlet.2020.02.013. Toxicology Letters 326 (2020) 83-98. 8. Chen Liang, Yan Gao, Yuyang He, Yongli Han, Ram Kumar Manthari, Chiranjeevi Tikka, Chenkai Chen, Jundong Wang, Jianhai Zhang. Fluoride induced mitochondrial impairment and PINK1- mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies. Environmental Pollution, 256 (2020) 113438. https://doi.org/10.1016/j.envpol.2019.113438 9. Yongli Han, Chen Liang, Ram Kumar Manthari, Yuxiang Yu, Yan Gao, Yu Liu, Shanshan Jiang, Chiranjeevi Tikka, Jundong Wang, Jianhai Zhang. Arsenicinfluences spermatogenesis by disorganizing the elongation of spermatids in adult male mice, Chemosphere, 238 (2020) 124650 https://doi.org/10.1016/j.chemosphere.2019.124650

10. Yan Gao., Chen Liang., Jianhai Zhang., Junjie Ma., Jinming Wang., Ruiyan., Niu., Chiranjeevi Tikka, Yewei Wang Jundong Wang. Combination of Fluoride and SO2 Induce DNA Damage and Morphological Alterations in Male Rat Kidney, Cell Physiol Biochem, (2018) 734-744. https://doi.org/10.1159/000494239.

11. Tikka Chiranjeevi, Osuru Hari Prasad, Uppu Venkateswara Prasad, Avula Kishor Kumar, Veeraraghavulu Praveen Chakravarthi1, Paramala Balaji Rao, Potuguchi Venkata Gurunadha Krishna Sarma, Nagi reddy Raveendra Reddy, Matcha Bhaskar. Identification of microbial pathogens in Periodontal disease and Diabetic patients of South Indian population, Bioinformation, (2014)10(4). doi: 10.6026/973***********.

12. Chiranjeevi Tikka, Hari Prasad Osuru, Navya Atluri, Praveen Chakravarthi Veera Raghavulu, Nanda Kumar Yellapu, Ismail Shaik Mannur, Uppu Venkateswara Prasad, Sudheer Aluru, Narasimha Varma K, Matcha Bhaskar. Isolation and Characterization of EthanoL Tolerant Yeast Strains. Bioinformation, (2013): 421-425. doi: 10.6026/973*********** 13.Kishore Kumar Avula, Hari Prasad Osuru, Sudheer Aluru, Mohan Kumar Vinay Chavan, Chiranjeevi Tikka, Bhaskar Matcha, P.V.G.K. Sarma, Alahari Papa Rao, Ravindra Reddy Nagireddy. Identification of predominant subgingival microbes in acute myocardial infarction patients of South Indian population, JICC, (2013) 104-110. https://doi.org/10.1016/j.jicc.2013.06.002. 14.O. Hari Prasad, A. Navya, D. Vasu, T. Chiranjeevi, M. Bhaskar, K.V. Sreedhar Babu, P.V.G.K. Sarma. Protective effects of Prosopis juliflora against Staphylococcus aureus induced hepatotoxicity in rats, Int J Pharm Biomed Res, (2011), 172-178.

15. V. Praveen Chakravarthi, Y. Nanda Kumar, K. Mahamanjunath, A. Rukmini, D. Bhavani, J. A. Pradeep Kiran, T.Chiranjeevi, M.Ismail, J. Durga Prasad, M. Bhaskar. Analysis of polymorphism in Kir 6.2 gene in Type 2 diabetic. Asian Journal of Biomedical and Pharmaceutical Sciences, (2014) 15-20. DOI:10.15272/ajbps.v4i37.584

16. Sudheer Aluru, Ismail SM, Kishore Kuamr A, Chiranjeevi T, Matcha Bhaskar and Papa Rao A. Enumeration of anaerobic periodontal pathogens from tuberculosis and chronic periodontitis patients by paper points and curettes: CFU based evaluation IJOMAS, (2014), 1- 9. Reference-1

Prof. Huda Salman, MD, PhD

Director, Brown Center for Immunotherapy

Don Brown Professor of Immunotherapy

Professor of Medicine and Microbiology & Immunology Co-Leader, HHM CC Program

975 W. Walnut St., IB554A’

Indianapolis, IN 46202

Email: *******@**.***

Office:317-***-****

cell: 317-***-****

Reference-2

Prof. Pravin Kaumaya, PhD,

Department of Microbiology and Immunology, School of Medicine, Indiana University, Director, Brown Center for Tumor Vaccine Core,