DNA manufacture and Associate Production manger

Safaa Khairi

• Cell: 571-***-**** • *****@**********.***

PROFESSIONAL SUMMARY

Organized, motivated and innovative molecular biologist with specialization in molecular genetics, cell biology, and biochemistry. Expertise in development and execution of genetic and biochemistry-based research methods. Ardent about learning new scientific skills from fellow team members EDUCATION

Master of Science: Biochemistry and Molecular Biology, May 2017 Georgetown University - Washington, DC

Thesis: “Roll of Prominin I (CD133) in tumor initiation and Metastasis of BRAFWT/NRASQ61K mutated

melanoma cells.”

Bachelor of Science: Medicine and Applied Medical Science, 2007- 2011 Umm Al Qura University - Makkah, KSA

EXPERIENCE

DNA Manufacture Manager and Associate Production Manager 11/2019 to present CytoTest Inc. – Maryland, Rockville

• Performs general molecular techniques such as plasmid purification, gel-electrophoresis, DNA/RNA isolation, Western blot, ELISAs, basic cell transfection experiments, and basic flow cytometry.

• Prepares research summaries, provides input to interpretation of experimental results, and troubleshoots general technical problems.

• Maintains cell lines for experiments and freezes cells.

• Works with scientific managers to ensure all internally generated data is correctly recorded and archived

• Prepares and sends laboratory reagents and supplies for instrument demonstrations to support domestic and international field application scientists.

• Works cooperatively and effectively with others both within and between departments.

• Uses scientific literature to keep aware of current technologies.

• Attends and presents results at department meetings and may present at company meetings.

• Maintains inventory and orders lab supplies.

• Maintains, calibrates and operates equipment according to established protocols. Monitors equipment for appropriate operation within set parameters and refers abnormal results to lab management.

• Complies with all applicable policies regarding health, safety and environmental policies.

• Other duties as assigned.

Lab Technician and Production,01/2018 to 11/2019

Extraction of the DNA using Qiagen plasmid Maxi or Midi kit. Production of FISH probe.

Manage GMP folder, stock in the DNA and glycerol.

Quantification of the DNA using Nano-Drop machine

Deals with machines such as incubator shaker, water bath, centrifuge, Autoclave, Nano-drop and vacufuge and run calibration for them Prepare different kinds of media and plates.

Wash glassware and Autoclave them.

Barista at Starbucks 09/2019 to 10/2020

Starbucks Pike 7 Plaza- Virginia, Vienna

Greeting customers in a friendly manner and taking food and drink orders Preparing and serving beverages

Preparing and serving food items such as sandwiches and pastries Answering customers' questions about menu choices Taking payment from customers

Maintaining inventory and equipment by cleaning and troubleshooting Keeping the work environment sanitary and organized Evaluating and modifying processes to improve efficiency and quality of service Internship, 06/2016 to 08/2017

Georgetown University – Washington, DC

Conducted research that tests and analyzes the feasibility, design, operation and performance of equipment, components and systems.

Performed Cell culture, immune technique, western blot, migration assays, times lapse, animals (mice and zebra-fish) injections with melanoma cells and using different microscopes such as inverted, Olympic and mastro for in vivo studies. Blood Bank Technology Specialist, 05/2015 to 01/2016 Ministry of interior, Security Forces Hospital Laboratory – Makkah, KSA Tested for blood group antigens, compatibility and antibody identification.

Investigated abnormalities such as hemolytic diseases of the newborn, hemolytic anemias and adverse reactions to transfusion. Supported physicians in transfusion therapy for patients with coagulopathies. Performed blood collection and processing, and donor selection.

Perfumed blood typing and molecular testing.

Analyzed donor blood for different kind of diseases. Performed viral marker testing to ensure patient safety, collected blood bone marrow, analyzed it under microscope and made films on slides. Conducted tests, inspections and maintenance of equipment, products, services, and processes for quality assurance.

Internship, 05/2010 to 05/2011

King Fahd Armed Forces Hospital – Jeddah, KSA

Fractionation, transduction and conjugation

Prepared and identified mutants with mutagenic chemicals. Internship Training, 06/2009 to 08/2009

King Khalid National Guard Hospital – Jeddah, KSA

Summer training in Blood Bank and hematology.

Tested blood groups, cross matched patient blood with donor blood to check compatibility.

Internship Training, 06/2008 to 08/2008

Alawi Tuesi and Bros Hospital – Jeddah, KSA

Summer training in Biochemistry and Hematology.

Successfully used cobas machine to run different blood tests. Analyzed urine and semen samples and performed HIV tests. SKILLS & ABILITIES

Hematology

Hem-agglutination and hemolysis assay, inoculation using inculcation bodies to obtain specific infections. Lymphocyte culture based on blood original lymph, Purify blood coagulations factors.

Microbiology

Prepared media such as blood media, GFA media and special media, pour plates in certain types of containers to study the types of infections. Plasmid amplification from already made plasmid, prepare and assay bacteriophage lysate. Histology

Routine frozen sections of whole animal brains, cell and fiber stains. Perfusion of animals, staining processes such as PAP satin and H&E satin. Embedding processes; paraffin, colliding and classification. Autoradiography of tissue sections. Plaque assay for antibody forming cell.

Serology

Florescent antibody technique using fluorescent tag, Spectrofluorometric. Isolate viruses from human tissues. Cytotoxicity assay to study kinds of toxic body. Maintained and purified bacteria, phage stocks, cell culture, tissue culture, culture transfers from human tissues to culture media. Blood Bank

Identified blood types and antibodies. Tested blood for viruses that might be transmi ed during transfusion. Investigated harmful responses of the body to blood transfusion. Supervising the collection, separation, delivery and storage of blood components. Supported physicians and nurses in blood transfusion therapy

Biochemistry

Determinations: RNA, protein, nucleic acid, phosphate and others. Gradients: Density, equilibrium.

Assays: enzyme, infectivity. Spectrophotometers: UV, infrared, atomic absorption. Electrophoresis: Immune, acrylamide gel, paper. Chromatography column: ion exchange resins, gel, gas liquid, HPLC, immunoprecipitation techniques. Analyze peptides, lipids. Manipulate DNA, equilibrium sedimentation and centrifugation. Prepare antibodies, synthesize peptides, and test for mutations, cell fractionation, transduction and conjugation. Prepare and identify mutants with mutagenic chemicals

RESEARCH

2016-2017, Role of Prominin I (CD133) in tumor initiation and metastasis of BRAFWT/NRASQ61Kmutated melanoma cells supervised by Dr. Cynthia Rosenthal. January 2009 – January 2010 Scientific Miracles in the Treatment by Genetic Engineering under supervision of Dr. Yusef Al-Maghrabi. November 2009 – October 2010 Retrospective study of Methicillin-resistant Staphylococcus aureus (MRSA) infections in King Abdul-Aziz university hospital, Jeddah. Supervised by Dr. Hani Faidah

LANGUAGES

Fluent in Arabic and English

AWARDS

26th December, 2015: Certificate of appreciation as a member of the blood bank department that helped during the disaster of pilgrims at Hajj 2015. 27th October, 2015: Certificate of appreciation at security forces hospital at Hajj 2015. 21st September, 2015: Organizer during international Blood Donation Day at Security Forces Hospital in Makkah, Saudi Arabia.

17th May, 2012: Certificate of a endance at the Research Proposal Course, held at King Abdullah Medical City.

14th June, 2011: Organizer during International Blood Donation Day at King Fahad Armed Forces Hospital in Jeddah, Saudi Arabia. 20th, April 2009: Organizer during Suitable for 25 years to create the Department of Laboratory Medicine and Quality Control in Diagnostic Medical Laboratories Workshop, Umm Al-Qura University, Makkah, Saudi Arabia.

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