September ****

Personal information:

First Name: Mahdiyar

Surname: Iravani Saadi

Sex: male

Date of Birth: January 16, 1980

Marital Status: Single

Current position:

Address:

Hematology Research Center, Shiraz University of Medical Sciences, P.O. Box: 71933-5899, Shiraz, Iran.

Tel: +98-71-361*****, Fax: +98-71-362*****, E-mail: adpq1a@r.postjobfree.com Education:

Ph.D ( Hematology)_(2014-2019): Hematology Research Center,, Shiraz University of Medical Sciences, Shiraz, Iran

Publications:

A-( Publications with first author, were shown by highlighted (Yellow color) B-( Publications with Corresponding author, were shown by highlighted (Green color) 1- Dysregulated Expression of miR-19b, miR-25, miR-17, WT-1, and CEBPA in Patients with Acute Myeloid Leukemia and Association with Graft versus Host Disease after Hematopoietic Stem Cell Transplantation. Mahdiyar Iravani Saadi, Raha Rahimikian, Zahed Karimi, Maryam Owjfard, Fatemeh Tahmasebijaroubi, Ahmad Niknam, Ehsan Nabi Abdolyousefi, Sanaz Salek, Reza Tabrizi, Elham Jamali . South Asian Journal of Cancer.October, 2021 (In printing).

2-MiR-181a and -b Expression in Acute Lymphoblastic Leukemia and Its Correlation with Acute Graft-Versus-Host Disease after Hematopoietic Stem Cell Transplantation, COVID-19 and Torque Teno Viruses. Mahdiyar Iravani Saadi, Mani Ramzi, Zahra Hesami, Nadiya kheradmand, Maryam Owjfard, Ehsan Nabi Abdolyousefi, Zahed Karimi. VirusDisease October 25, 2021.

3-Cloning of Bacillus Subtilis Phytase Gene Construct in Escherichia coli".Mahdiyar Iravani Saadi, Abbas Doosti, Heeva Jalali; Ehsan Nabi Abdolyousefi, Mansooreh Hooshiyar, Reza Tabrizi; Esmat Noshadi. Iranian Journal of Microbiology. Volume 13 Number 5 (October 2021) 664-670.

4-Gold Nanobiosensor Based on the Localized Surface Plasmon Resonance is Able to Diagnose Human Brucellosis, Introducing a Rapid and Affordable Method. Sina Vakili

,Mohammad Samare-Najaf,Amirreza Dehghanian,Amir Tajbakhsh, Hassan Askari,Reza Tabrizi,Mahdiyar Iravani Saadi, Ahmad Movahedpour, Marzieh Alizadeh, Ali Samareh

,Saeed Taghizadeh,Saam Noroozi. Nanoscale Research Letters. 2021 Sep 16;16(1):144. doi: 10.1186/s11671-021-03600-4 .

5-Opium use and risk of stroke: A systematic review and meta-analysis. Afshin Borhani Haghighi, Reza Tabrizi, Mehrdad Estakhr, Mahnaz Hosseini-Bensenjan, Maryam Niknam, Mahdiyar Iravani Saadi, Fatemeh Moazzen, and Peyman Nowrouzi-Sohrabi. Heroin Addiction And Related Clinical Problems .August 14, 2021(In printing). 6-Expression levels of Il-6 and Il-18 in acute myeloid leukemia and its relation with response to therapy and acute GvHD after bone marrow transplantation. Mahdiyar Iravani Saadi, Mani Ramzi, Mehran Hosseinzadeh, Narjes Ebrahimi,Maryam Owjfard, Ehsan Nabi Abdolyousefi, Zahra Hesami, Behnaz Valibeigi, Neda Zareei, Fataneh Tavasolian, Hourvash Haghighinejad, Abdolhossein Zare. Indian Journal of Surgical Oncology. May 2021. 7-Expression of Interleukin 1, Interleukin 27 and TNF α Genes inPatients with Ischemic Cardiomyopathy Versus IdiopathicDilated Cardiomyopathy. Mahdiyar Iravani Saadi, Javad Salami, Hanieh Abdi, Ehsan Nabi Abdolyousefi, Ahmad Niknam, Zahed Karimi, Mohammad Ali Babaee Beigi, Abbas Mohammadi, Alireza Manafi. BMC Cardiovascular Disorders.2021( In printing).

8-The Immune Response and Effectiveness of COVID-19 Therapies. Fataneh Tavasolian

,Gholam Reza Hatam,Sayed Hussain Mosawi, Mahdiyar Iravani Saadi,Elham Abdollahi

,Tannaz Jamialahmadi,Thozhukat Sathyapalan,Amirhossein Sahebkar. March 2021.Advances in Experimental Medicine and Biology 1321:115-126. 9-Characterization of Mir-92b, 1275 and 551 in Patients with Acute Myeloid Leukemia and Their Association with Acute Graft Versus Host Disease after Hematopoietic Stem Cell Transplantation. Mahdiyar Iravani Saadi, Haleh Bozorgi, Mani Ramzi1, *, Esmat Noshadi, Arvand Akbari,Iman Jamhiri, Hourvash Haghighinejad, Zahed Karimi, Mohsen Nikandish.International.journal of hematology and therapy. February 07, 2020. 10-Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia . Mahdiyar Iravani Saadi, Nargess Arandi, Ramin Yaghobi, Negar Azarpira, Bita Geramizadeh, Mani Ramzi. Indian J Hematol Blood Transfus.2019 .

11- Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Association with Development of Graft versus Host Disease after Hematopoietic Stem Cell Transplantation . Mahdiyar Iravani Saadi, Mani Ramzi, Ramin Yaghobi, Nargess Arandi. International Journal of Organ Transplantation Medicine. Int J Org Transplant Med 2019; Vol. 10 (2) .84-90.

12-The circulating level of interleukins 6 and 18 in ischemic and idiopathic dilated cardiomyopathy .Mahdiyar Iravani Saadi, Mohammad Ali Babaee Beigi, Maryam Ghavipishe, Maryam Tahamtan, Bita Geramizadeh, Abdolhossein Zare, Ramin Yaghoobi.J Cardiovasc Thorac Res, 2019, 11(2), 132-137doi: 10.15171/jcvtr.2019.xx. 13- Genetic Variation of TNF-α and IL-10, IL-12, IL-17 Genes and Association with Torque Teno Virus Infection post Hematopoietic Stem Cell Transplantation . Mani Ramzi, Nargess Arandi, Tahereh Zarei, Mahdiyar Iravani Saadi, Ramin Yaghobi, Mohamad Moghadam1, Bita Geramizadeh. Acta virologica 63: 186 – 194, 2019. (Corresponding author: Mahdiyar Iravani Saadi).

14 -Up-Regulation of the miR-92a and miR-181a in Patients with Acute Myeloid Leukemia and their Inhibition with Locked Nucleic acid (LNA)-antimiRNA;Introducing c-Kit as a New Target Gene. Mahdiyar Iravani Saadi, Nargess Arandi, Ramin Yaghobi, Negar Azarpira, Bita Geramizadeh, Mani Ramzi. International Journal of Hematology and Oncology(UHOD). 2018; 4(28).

https://doi.org/10.1007/s12288-018-01066-6

15- Mutation of the DNMT3A and IDH1/2 genes in Iranian acute myeloid leukemia patients with normal karyotype (CN-AML): association with other gene mutation and clinical and laboratory characteristicsNarjes . Mahdiyar Iravani Saadi, Tahereh Zarei, Mani Ramzi, Nargess Arandi. Journal of Hematopathology (2018) 11:29–36. 16-Genetic Variation of Costimulatory Molecules, Including Cytotoxic T-Lymphocyte Antigen 4, InducibleT-Cell Costimulator, Cluster Differentiation 28, and Programmed Cell Death 1 Genes, in Iranian Patients With Leukemia. Mani Ramzi, Nargess Arandi, Mahdiyar Iravani Saadi, Ramin Yaghobi, Bita Geramizadeh .Experimental and Clinical Transplantation

(2018).( Corresponding author: Mahdiyar Iravani Saadi). 17- Association Between Cytotoxic T-Lymphocyte Antigen 4 Gene Polymorphisms and Torque Teno Virus Infection After Hematopoietic Stem Cell Transplantation.Mani Ramzi, Mahdiyar Iravani Saadi, Tahereh Zarei, Ramin Yaghobi, Nargess Arandi1. Experimental and Clinical Transplantation (2018).

18-Association between TLR2 and TLR4 Expression and Response to Induction Therapy in Acute Myeloid Leukemia Patients. Mani Ramzi, Abolfazl Khalafi-Nezhad, Mahdiyar Iravani Saadi, Zahra Jowkar. International Journal of Hematology-Oncology and Stem Cell Research 12(4) - October, 1, 2018.

19- Leukemia microvesicles affect healthy hematopoietic stem cells. Farnaz Razmkhah, Masoud Soleimani, Davood Mehrabani,Mohammad Hossein Karimi, Sedigheh Amini Kafi- abad, Mani Ramzi, Mahdiyar Iravani Saadi, and Javad Kakoui. Tumor Biology .February 2017: 1–8-.

20- The Association Between Viral Infections and Co-stimulatory Gene Polymorphisms in Kidney Transplant Outcomes. Ahmad Niknam, Mohammad Hossein Karimi, Ramin Yaghobi, Bita Geramizadeh,. JamshidRoozbeh, Mehdi Salehipour, and Mahdiyar Iravani. Jundishapur J Microbiol. 2016 August; 9(8):e31338. 21- Study of the Associations Between TT Virus Single and Mixed Infections With Leukemia. Marjan Shaheli, Ramin Yaghobi, Abbasali Rezaeian, Mahdiyar Iravani Saadi, Mani Ramzi . Jundishapur J Microbiol. 2015 April; 8(4): e18212. 22-Association Between TTVirus Infection and Cirrhosis in Liver Transplant Patients. Mohammad Javad Kazemi, Ramin Yaghobi,Mahdiyar Iravani Saadi,Bita Geramizadeh, Javad Moayedi, Hepat Mon. 2015 Sep 27;15(9):e28370. doi: 10.5812/hepatmon.28370. eCollection 2015 Sep.

23- Polymorphism of costimulatory molecules (CTLA4, ICOS, PD.1 and CD28) and allogeneic hematopoietic stem cell transplantation in Iranian. Mahdiyar Iravani-Saadi, Mohammad Hossein Karimi,Ramin Yaghobi, Bita Geramizadeh, Mani Ramzi, Ahmad Niknam, and Arefeh Pourfathollah. Immunological Investigations, 2014; 43(4): 391–404- 24- Relation Between Costimulatory Molecule Polymorphism and Hepatitis B Infections in Hematopoietic Stem Cell Transplant Recipients. Ramin Yaghobi, Mahdiyar Iravani Saadi, Mohammad Hossein Karimi,Bita Geramizadeh,Mani Ramzi, Maryam Zakerinia. Experimental and Clinical Transplantation (2014) 4: 357-366. 25- Association of the costimulatory molecule gene polymorphismsand active cytomegalovirus infection in hematopoietic stem cell. Mahdiyar Iravani Saadi, Ramin Yaghobi, Mohammad Hossein Karimi, Bita Geramizadeh,Mani Ramzi, Maryam Zakerinia. Mol Biol Rep (2013) 40:5833–5842.

TEACHING EXPERIENCE

1. Teaching Assistant, Laboratory Course Sept. 2014-Sept. 2018 2- Good Laboratory Practices(GLP). 2 October, 2017.Held by Shiraz Transplant Research Center

3- Teaching Assistant,Molecular Biology:

A. Culture and Maintenance of Mouse Embryonic Stem Cells B. Genetic Engineering Techniques(Transfection)

C. Real-time PCR (Shadowing)

D. PCR, Gel Electrophoresis & Gel Documentation

E. Flowcytometry

F. Principles of Primer Design

G. Cell culture

H. MTT assay

In Collaboration with

1- Division of Hematology/Oncology-Pediatric Department, School of Medicine,Amir Oncology Hospital-Hematology/Oncology Ward, Shiraz University of Medical Sciences, Shiraz

2- Transplant Research Center, Shiraz University of Medical Sciences, Shiraz

3- Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz

4-Cardiovascular Research Center, Shiraz University of Medical Sciences,

RESEARCH INTEREST (My Research Interests is Focused on LEUKEMIA and LYMPHOMA)

1- Gene Therapy Using Nanobiomaterials based Microfluidic System 2- Gene Therapy Using LNA-anti-miRNA/mimic in the treatment of malignancies. 3- Gene Therapy Using SiRNA in the treatment of malignancies. 3-Gene Editing based on CRISPR Technology for Cell Transplantation 4- Hematopoietic Differentiation from Embryonic Stem Cells and induced Pluripotent Stem Cells for Stem Cell Transplantation

5- Using Autophagy in to limit blast cell survival and reduce leukemic cell proliferation in AML patients.

TRAINING COURSE

1. The se cond national Workshop on Haemostasis Tests among targeted lab in Iran March 2019,shiraz,Iran

2-Microfluidics March 14, 2018

Held by Council for Development of Stem Cell Sciences and Technologies in Collaboration with Stem Cell Technology Research Center, Tehran 3-Transmission Electron Microscopy (TEM) March 14, 2018 Held by (SUCL)niversity, National Institute of Genetic Engineering and Biotechnology, 4-Fluorescence Microscopy and its Application Held by (SUCL)niversity, March 14, 2018

5-Synthesis of Solution and Buffer Held by General Chemistry Laboratory, Department of Chemistry, Shiraz University Central Laboratory. Sept. 292013 Stem Cells

1. Familiarity with Production and Maintenance of Induced Pluripotent Stem Cells (iPS Cells)

Held by Epigenetic Reprogramming Group, Department of Stem Cell Biology and Technology, Royan Institute, Tehran March. 2015

2. Isolation and Culture of Umbilical Cord Blood, Peripheral Blood and Bone Marrow Mesenchymal Stem Cells

Held by Stem Cell Laboratory, Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shirazmay. 7, 2015 3. Isolation and Characterization of Cancer Stem Cells April., 2015 4. Held by Cancer and Hematopoietic Stem Cells Group, Department of Stem Cell Biology and Shiraz Transplant Research Center . April., 2014 5. Culture and Maintenance of Mouse Embryonic Stem Cells Jan. 28, 2016 6. Held by Biology of Pluripotent Stem Cells Group, Department of Stem Cell Biology and Shiraz Transplant Research Center . may 2014

Culture of Peripheral Blood Mononuclear Cell (PBMC) Sept. 30, 2015 Held by Research Consultation Center at Burn Research Center in Collaboration with Molecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz.

Molecular Biology

1. Regenerative Gene Therapy with CRISPR Technology Sept. 5, 2016 Held by Stem Cells Preparation Department and Ophthalmology Research Center of Farabi Hospital-Tehran University of Medical Sciences in Collaboration with Council for Development of Stem Cell Sciences and Technologies, Tehran 2. Genetic Engineering Techniques(Transfection) may, 2013 Held by Plant Bioproducts Department, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran 3. Real-time PCR (Shadowing) June 6, 2015

Held by Molecular and Cytogenetic Laboratory, Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 4. PCR, Gel Electrophoresis & Gel Documentation June 6, 2015 Held by Molecular and Cytogenetic Laboratory, Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 5. Flowcytometry December, 2012

Held by 4th Postgraduate Summer School, Shiraz University of Medical Sciences, Shiraz and Shiraz Transplant Research Center

6. Flowcytometry (Shadowing) June, 2015

Held by Molecular Pathology Laboratory, Department of Pathology, Shiraz University of Medical Sciences, Shiraz

7. Principles of Primer Design June, 2014

Held by 4th Postgraduate Summer School, Shiraz University of Medical Sciences, Shiraz

8. 9- Principles of Primer Design . Held by and Bioinformatic, Shiraz Transplant Research Center. July, 2011

9. SDS-PAGE and Western Blotting May, 2015

Held by 4th Postgraduate Summer School, Shiraz University of Medical Sciences, Shiraz and Shiraz Transplant Research Center

Paper Writing

1. Systematic Review: an Introduction May 31-June 1, 2016 Held by Research Consultation Center in Collaboration with The International Journal of Occupational and Environmental Medicine, Shiraz University of Medical Sciences, Shiraz

2. Advanced Scientific Writing july., 2015

Held by Research Consultation Center in Collaboration with The International Journal of Occupational and Environmental Medicine, Shiraz University of Medical Sciences, Shiraz

3. How to Read and Understand a Scientific Paper Nov. 13-14, 2017 Held by Research Consultation Center in Collaboration with The International Journal of Occupational and Environmental Medicine, Shiraz University of Medical Sciences, Shiraz

4. EndNote Software-Advanced Level July, 2011.Held by Shiraz University of Medical Sciences, Shiraz

5. Advanced PubMed October, 2011

Held by Research Consultation Center, Shiraz University of Medical Sciences, Shiraz and Shiraz Transplant Research Center.

6. Basic Research Methodology(Design,Metod,Academic Ethics October, 2017 Held by Research Consultation Center in Collaboration with Department of Community Medicine-School of Medicine, Shiraz University of Medical Sciences, Shiraz

7. Computer Operator ICDL-Grade 1 & 2 October, 2017.Held by Shiraz Transplant Research Center

Statistics

1. Design and Analysis of Clinical Trial Nov. 25, 2017 Held by Research Consultation Center in Collaboration with Department of Biostatistics-School of Medicine, Shiraz University of Medical Sciences, Shiraz 2. The Design and Analysis of Experiment July 18, 2013 Held by Research Consultation Center in Collaboration with Department of Community Medicine-School of Medicine, Shiraz University of Medical Sciences, Shiraz

3. SPSS Software (Introduction and Application) Nov. 23, 2014 Held by Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz

4. GraphPad Prism Software (Introduction and Application) Nov. 30, 2015 Held by Research Consultation Center in Collaboration with Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz English Language

1. Graduated in TOLIMO 1 & 2 Courses May. 2017- University of Tehran Language Center NO.3, Faculty of Foreign Languages and Literatures, University of Tehran, Tehran

SKILL

Instrumental

Microscopy

1- ASSY PRIFERAL BLOOD AND BONE MARROW LAM

Transmission Electron Microscopy and Take Picture and Analyzing the Images Fluorescent Microscopy, Inverted Microscopy, Optical Microscopy and Stereo Microscopy: Work with Device, Taking Images and Analyzing

Molecular Biology

Real time-PCR, Flowcytometry, PCR, Gel Electrophoresis, Gel Documentation and Plate Reader (ELISA): Working with Device and Software, Interpret the Data Technique

Synthesis & Engineering System

, RNA Isolation, cDNA Synthesis, Primer Designing, PCR, Electrophoresis, Gene Cloning, SDS-PAGE, Protein Expression and Purification

Laboratory Animals

Animal Welfare and Animal Ethics, Husbandry, Handling (Injections, Blood Sampling …), Anatomy of the Rat, Euthanasia, Anesthesia, Necropsy, Tissue Sampling, Gavage and Suture

Biological

Derivation and Preparation of MEF, SDS-PAGE and Western Blotting, IHC Technique, MTT Assay, Magnetic Cell Sorting, Transfection & Transduction, Neubauer Chamber Cell Counting, Cell Culture & Passaging, Electroporation and Staining

Statistical Software

SPSS

SmartDraw 2013

Rest2009

MedCalc 8.0

Morpheus Heatmap

GraphPad Prism 6 (GraphPad Software, Inc., La Jolla, CA, USA)

Arlequin v 311

linkage disequilibrium(LD)

Hardy–Weinberg was evaluated using Arlequin V311. bioinformatic

A. ensemble

B. gene in NCBI

C. GeneCards

D. Primer Design(Allele ID 7.5-Becone Ddsigner-Primer 6-MEGA 5-Gene Runner.) E. Direct sequencing was performed with each gene corresponding forward primer after PCR amplification using ABI Prism 3730XL/ FinchTV/Chromas DNA sequencing analyzer.

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