Birth: **** Washington Minot, Thomas ND USA Street, • DOB: San 30.Francisco, 11.Anthony 1994 CA • Nationality: 94018 • (316) South Perkins, 305-Korean-8304 • American tperkins313@Jr. • Gender: gmail.com Male Profile

Currently Assembly/working Maxiprep. for My Twist education Bioscience and job as a experience Manufacturing has exposed Team Lead me in to Gene a multitude of proactive, instruments/passionate, techniques science-utilized based, in and research innovative. and high-I am throughput looking for production. an opportunity I am to a develop Passionate my about skills new and to advancements learn more every in synthetic day. Helping biology. people is what motivates me. Skills Molecular that translate cloning, Primer to a Research design to Associate’s ensure specificity job duties (Degenerative are: and Nested), CHO cell insulin viability (bioreactor)assay, Maintenance, of of CHO KEX2 cell restriction culture that enzyme was used to cut to pro-express insulin pro-

(restriction-bioreactor)enzymes,, Chromatography Ligation, Transformations, via Hydrophobic interaction, Analysis of SPR Western data, Blot, Analysis PCR, of Use of Dynamic reviewing Light and keeping Scattering up and to date Static with Light influential Scattering academic data for literature proteins, relevant Excited to to learn, antibody Grit, drug Persistent discovery in and phage display.

Lab Organic experience Chemistry, in: Physical Chemistry, Biotechnology, Aquatic Toxicology, Quality Control labs for DNA samples, Microbiology, Gene Assembly lab, Molecular Biology, Reagents. Education

Harvard Genetics, Solano Associate Prior to College 2017, Immunology, Medical of Science I spent School and 4 and years Associate - Pharmacology HMX spread of across Arts in University Chemistry of Kansas for Nursing and University of Hawaii enrolled Vacaville, Boston, 2017-Maui 2021 2019 MA CA for Sustainable Science Management is in Chemistry/from Biotechnology 2013 – 2017. which Throughout I am actively my university seeking a career experience, in. I found my passion Projects • Multistep Reaction Sequence: Benzaldehyde to Benzilic Acid; use of thiamine hydrochloride “Green” reagent for catalysis of benzaldehyde to benzoin. o Thiamine Oxidation hydrochloride of benzoin to benzil was used using as a a mild catalyst oxidizing to mend agent, two nitric moles acid(of benzaldehyde HNO3). Finally, into a benzoin. rearrangement hydroxide(KOH)reaction . Crystallization to form benzilic and purification acid from of benzil each solid using was the performed strong base to potassium analyze and

• Cloning calculate each of conservative step, compound was purified gene, yield, through melting GAPDH, ethanol point, through washes and other use and of physical examined isolated properties. after genomic drying. The DNA crude from substance from Arabidopsis thaliana, Solanum lycopersicum, and Daucus carota as means of source of gene.

o Specific was then plant used material for extraction was chosen of the for highly-genomic conserved DNA isolation, GAPDH gene carrot through (Dc) and “Nested” tomato PCR. (Sl) Isolating which genomic Concentration DNA from of DNA plants: and Quality Harvest, Check. Grind, After Lyse, genomic Removal DNA of Cellular is successfully Debris, Purification isolated, continued of DNA,

• Exploration o working primers. Primer § § of Design: First nucleotide Second “nested” with Checked Diversity round the Round primers gDNA the sequences of amongst concentration PCR PCR to that amplify with with typically select proteomics “Degenerative” “Nested” our and only desired surround purity for primers sequences of target of Fish primers GAPDH, genomic to gene using select that GAPC designed sequence, DNA hold SDS-against through gene the PAGE. from GAPDH, to GAPDH false amplify gel a consensus positives electrophoresis. gene. using target degenerate of by sequence. utilizing o Immunoblotting followed SDS-PAGE to amplify specific proteins on the nitrocellulose membrane.

• Detection Birth: 1085 Washington Minot, of the Thomas ND “Jumping USA Street, • DOB: San Gene,30.Francisco, 11.Anthony ” 1994 Human CA • Nationality: 94018 PV92 • (316) Alu South Perkins, 305-Insertion. Korean-8304 • American tperkins313@Jr. • Gender: gmail.com Male o Tested are short for DNA PV92 sequences Alu insertion consisting in a population of 300 bp. of Found 30 random 11 of 30 participants. participants The had PV92 the Human Alu elements PV92

• • Ligation Green Fluorescent Alu of insertion purified in Protein PCR their product genome. purification (GAPDH) via into Hydrophobic pJet 1.2 plasmid Interaction vector. Chromatography.

• Transformation of pGLO DNA plasmid into Escherichia coli (HB101). o Introduced pGLO plasmid a foreign DNA contains: gene into Origin Escherichia of Replication Coli (Ec) (ori)HB101, β-lactamase cells using (bla pGLO or amp)plasmid, multiple DNA. The cloning expression sites plasmid (MCS), due cytosine to in arabinoside order to express (araC) recombinant and the Green proteins. Fluorescent With the Protein help of (GFP)bla . Used an restrictive did not contain enzyme, the plasmid was able through to separate the process host cells of containing selection. The expression colonies plasmid containing and the those GFP that gene contains: in the transformation expression plasmid solution, inside pGLO the plasmid host cell DNA, should arabinose, be isolated ampicillin, in the LB and agar LB plate agar. that This can be observed using a UV light underneath the +pGLO ara/amp/LB agar plate. Transformation

• 2017 The in 1999. World World steps: The Youth event Youth CaCl2 Congress is and for Congress young heat is an shock leaders international - Aloha+ to insert to come Challenge plasmid event together hosted into hosted and E. in coli. discuss a multitude by United world of issues. countries Nations. As the since vice its president beginning of the members SOS club from in each Maui, came I represented together to our collaborate school in the on Aha how Ho’owaiwai we would model on Oahu. the lessons During this and event, plan each club day during the Aloha+ the World challenge Youth as a Congress reference which when took formatting place in each June, day 2017. that Directed the delegates by Governor would be Ige, present we utilized for the event.

Certifications

• COVID-19 Contact Tracing -Johns Hopkins Bloomberg School of Public Health October 2020 o Credential ID: 738DTHJBHXKM

Clubs

• • Student Student Ohana Sustainability for Sustainability Coalition of – Hawaii Vice President – Representative of Maui 2016 - 2017 2017 Work Twist Bioscience Experience South San Francisco, CA Manufacturing Team Lead April 2020 - Present

As • Train a Team staff Lead on new for Maxiprep: processes and process changes.

• • Assist Coordinate in development manufacturing of new staff processes to achieve involved production in or goals. surrounding Maxiprep.

• • Oversee Identify process manufacturing failures processes and troubleshoot and prioritize errors. tasks according to production needs.

• • Create Implement Powerpoint and maintain presentations, 5S organization excel templates, in laboratory and tutorials spaces. for teaching team new methods.

• • Attend Involved to in staff New concerns Product and Introduction relay issues regarding to management. different plate/tube formats. Teaching/training for new team members:

- - Starter Creating Culture starter planning. cultures for all clonal gene orders from RCA plates.

- - Scaled-Trouble up shooting Qiagen on Maxiprep MES, Confluence, process. and Sample tracker software.

- liquid-Shipping handling methods machines. for 96 DWP, 1.4 Micronic tubes, 2mL tubes, and 4 mL Micronic tubes on Hamilton automated

- Methods on Hamilton to produce copies of our finished product for QC testing. Birth: 1085 Washington Minot, Thomas ND USA Street, • DOB: San 30.Francisco, 11.Anthony 1994 CA • Nationality: 94018 • (316) South Perkins, 305-Korean-8304 • American tperkins313@Jr. • Gender: gmail.com Male QC methods taught include:

- - DNA Preparation quantification of genes using for NGS a spectramax screening in by 96w transferring format. samples into Echo plates.

- a Endotoxin Charles River Assay PTS. by creating 1ng DNA/μL dilutions and testing the samples on an accompanying LAL cartridge in

- Twist Genomic Bioscience DNA(gDNA) quantification using gel electrophoresis and imaging software. South San Francisco, CA Manufacturing Associate January 2020 – Present

One have of developed the first five Midiprep members (10μg brought - 100μg onto DNA the yield) Maxiprep and Maxiprep team after (100μg the process - 1mg was DNA introduced. yield) into functional As a team, we products shipping methods offered by for Twist! our midi/Recently, maxi we clonal transitioned genes. from soft-launch to commercial-launch and we developed our Twist Molecular Bioscience: Biology Developed Revolution. novel I work silcon-in lab based that handles Oligo printing a multitude technology of processes which has spanning changed oligo the assembly face of the to Next Generation Sequencing.

As • Qiagen a Manufacturing Maxiprep Process. Associate for Maxiprep:

• • Experienced Collaboration using with MES, Antibody Confluence, Engineering Tableau, and Sample Gene Assembly Tracking lab. software.

• • Manufacture Follow SOPs to high ensure quality efficient custom and gene consistent products production. in a high throughput lab.

• • Operate Utilize a robotic variety of laboratory traditional equipment laboratory such equipment as automated including liquid bioanalyzers, handlers. centrifuges, thermocyclers, and spectrophotometers. • Perform large scale production and shipping in 48-well 2D tubes, 96-well Deep Well Plates, 96-well 1.4mL tubes, 2mL • Maintain tubes detailed and 384-record well formats. keeping and thorough documentation of manufacturing processes.

• • Prep Employ of samples sterile technique for Next Generation to prevent contamination Sequencing to ensure of products. quality of gene products.

• Qubit-based spectroscopy.

Pacific EcoRisk Cordelia, CA

Aquatic Toxicology Laboratory Assistant Oct 2018 – Sept 2019

• • Handling Shipping of of sample samples kits involving to clients. custody forms.

• • Performance Preparation of of synthetic routine test waters solution and test analyses solutions. (Dissolved Oxygen, pH, Conductivity, Alkalinity, Hardness, etc.)

• • Determination Pick-up and transport of organism of aliquot weights. and sediment samples.

• restocking Tasks related of workstations, to the maintenance cleaning of of a glassware clean, safe, and and facilities, unencumbered and other laboratory general housekeeping work environment, responsibilities. including

• • Experience Handling of with samples an abundance involving of custody freshwater forms. and marine species which allowed me to become proficient in animal husbandry, test maintenance, and data analysis in on going experiments for: Americamysis bahia, Ampelisca abdita, Chironomus dilutus, Crassostrea gigas, Cyprinidon variegates, Strongylocentrotus purpuratus, Haliotis rufescens, Hyalella azteca, Leptocheirus plumulosus, Lumbriculus varieagatus, Macoma nausta, Menidia beryllina, Mytilus spp, Neanthes arenaceodonta, Nereis virens, Oncorhynchus mykiss, Pimephales promelas, and Nephtys caecoides.

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