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ANTHONY WONG

** ******** ********, ***********, *******, M1V 4N7

E-mail: adgokb@r.postjobfree.com

Tel: 416-***-**** (Home) ꞏ 647-***-**** (Cell) EDUCATION

August 2011 M.Sc., Microbiology and Immunology, University of Western Ontario May 2008 B.Sc. (Hons), Molecular Biology and Microbiology, University of Toronto WORK EXPERIENCE

June 2015 – June 2020, Research Associate

Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore

May 2013 – June 2015, Technical Scientist

RIKEN Center for Life Science Technologies, Yokohama, Japan October 2011 – August 2012, Translator

Agelle PR Design and Media Production, Kyoto, Japan August 2008 – August 2009, Assistant Language Teacher Minakami Board of Education, Minakami, Japan

RELEVANT RESEARCH EXPERIENCE

June 2015 – June 2020

Singapore Centre for Environmental Life Sciences Engineering, Singapore Our project focuses on identifying microorganisms that are within the air microbiome, both indoors and outdoors. A metagenomics approach is employed to determine the composition of this understudied environment. Complementing this, classical microbiological, molecular biology and microscopy techniques are being adapted to corroborate the bioinformatic data. Additionally, a patient cohort with lung disease is currently being investigated to determine if a link between the air and lung microbiome exists.

Selected list of relevant skills and responsibilities

Investigating feasibility of single cell sorting and downstream whole genome sequencing

Responsible for organizing a joint research collaboration agreement between Nanyang Technological University and the University of British Columbia (patient cohort/air microbiome joint study)

Responsible for tender process for a new scanning electron microscope

Training of interns and new staff on sample preparation and microscope use (light/EM)

Using light and electron microscopy to further project goals Page 2 of 9

May 2013 – June 2015

RIKEN CLST, Division of Genomic Technologies, Yokohama, Japan In order to analyze and quantify differential gene expression at the single cell level, our team worked towards developing a proof of principle to quantify expression of multiple genes using in situ bridge PCR amplification coupled with reverse transcription. Using TIRF microscopy, detection of single DNA/RNA molecules was possible. Additionally, I concurrently worked on a project involving automated high throughput screening of anti-sense oligonucleotides in human iPS cells.

Selected list of relevant skills and responsibilities

Light microscopy (confocal, TIRF)

Sourcing of microscope equipment and basic maintenance

Culturing/maintenance of human iPS cells

September 2009 – August 2011

University of Western Ontario, London, Ontario

Thesis title: Role of capsular modified heptose in the virulence of Campylobacter jejuni. In this project, I investigated differential capsular expression in various Campylobacter jejuni strains and also the role modified heptoses play. The creation and characterization of modified- heptoseless C. jejuni mutants both genetically and phenotypically was also carried out. SUPERVISORY EXPERIENCE

2018 – 2020

Supervising Phu Pwint Thin Hlaing, hired as a research assistant, on a day-to-day basis 2017 – 2018

Supervised Phu Pwint Thin Hlaing and Merrilyn Eng, final year students from Temasek Polytechnic.

2016 – 2017

Supervised Christopher Tan, a final year student from Temasek Polytechnic. AWARDS AND ACHIEVEMENTS

2013

Awarded the Japanese Language Proficiency Test (JLPT) N1 Certificate 2006

Accepted as an Associateship of the Royal Conservatory of Toronto (ARCT) and conferred a performer’s diploma in piano with standing first class honours OTHER

Currently holding a valid N3 National Environment Agency (Singapore) license for operation of non-ionizing irradiation equipment

Fluent in 3 languages (English, Cantonese, Japanese) Page 3 of 9

LIST OF PUBLICATIONS

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Anjali Bansal Gupta, Nicolas E. Gaultier, Ngu War Aung, Rikky W. Purbojati, Elaine L. Oliveira, Anthony Wong, Deepa Panicker, Alexander Putra, Akira Uchida, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome Sequence of Penicillium oxalicum Strain SGAir0226 Isolated from Outdoor Tropical Air in Singapore. Mycopathologia. 2020 Jun;185(3):591-594. Akira Uchida, Hie Lim Kim, Rikky W. Purbojati, Vineeth Kodengil Vettath, Anjali Bansal Gupta, James N. I. Houghton, Caroline Chénard, Anthony Wong, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexander Putra, Cassie E. Heinle, Balakrishnan N. V. Premkrishnan, Ana Carolina M. Junqueira, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete genome of Enterobacter sichuanensis Strain SGAir0282 Isolated from Air in Singapore. Gut Pathog 12, 12

(2020). February 27 2020.

Namrata Kalsi, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Megan E. Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E. Gaultier, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Stephan C. Schuster. Draft Genome Sequence of Bacillus megaterium Type Strain SGAir0080. Microbiology Resource Announcements. 2019 Dec 12;8(50).

Phu Pwint Thin Hlaing, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexandra Putra, Carmon Kee, Nicolas E. Gaultier, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Serene B.Y. Lim, Vineeth Kodengil Vettah, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome Sequence of Curtobacterium sp. strain SGAir0471 Isolated from Singapore Air. Microbiology Resource Announcements. 2019 Nov 21;8(47).

Gusareva ES, Acerbi E, Lau KJX, Luhung I, Premkrishnan BNV, Kolundžija S, Purbojati RW, Wong A, Houghton JNI, Miller D, Gaultier NE, Heinle CE, Clare ME, Vettath VK, Kee C, Lim SBY, Chénard C, Phung WJ, Kushwaha KK, Nee AP, Putra A, Panicker D, Yanqing K, Hwee YZ, Lohar SR, Kuwata M, Kim HL, Yang L, Uchida A, Drautz-Moses DI, Junqueira ACM, Schuster SC. Microbial communities in the tropical air ecosystem follow a precise diel cycle. Proc Natl Acad Sci U S A. 2019 October 28.

Shruti Ketan Kutmutia, Daniela I. Drautz-Moses, Akira Uchida, Rikky Wenang Purbojati, Anthony Wong, Kavita K Kushwaha, Alexander Putra, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Ana Carolina Martins Junqueira, Stephan C. Schuster. Complete Genome Sequence of Micrococcus luteus Strain SGAir0127 Isolated from Indoor Air Samples of Singapore. Microbiology Resource Announcements. 2019 October 10; 8(41). Elaine Lopes Oliveira, Daniela I. Drautz-Moses, Akira Uchida, Rikky Wenang Purbojati, Anthony Wong, Kavita K Kushwaha, Alexander Putra, Ngu War Aung, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Ana Carolina Martins Junqueira, Stephan C. Schuster. Complete Genome Sequences of Rhodococcus sp. Strain SGAir0479, Isolated from Indoor Air Collected in Singapore. Microbiology Resource Announcements. 2019 October 3; 8(40).

Anthony Wong, Ana Carolina M. Junqueira, Ankur Chaturvedi, Akira Uchida, Rikky W. Purbojati, James N.I. Houghton, Caroline Chénard, Sandra Kolundžija, Megan E. Clare, Kavita K. Page 4 of 9

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Kushwaha, Nikappil S. Deepa, Alexander Putra, Nicolas E. Gaultier, Balakrishnan N.V. Premkrishnan, Vineeth Kodengil Vettath, Cassie E. Heinle, Daniela I. Drautz-Moses, and Stephan C. Schuster. Complete Genome Sequence of Lysinibacillus sp. Strain SGAir0095 Isolated from Tropical Air Samples Collected in Singapore. Microbiology Resource Announcements. 2019 September 19; 8(38).

Serene B. Y. Lim, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexander Putra, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Stephan C. Schuster. Genome Sequence of Pontibacter sp. Strain SGAir0037, Isolated from Tropical Air Collected in Singapore. Microbiology Resource Announcements. 2019 September 12; 8(37). Lakshmi Chandrasekaran, Daniela I. Drautz-Moses, Akira Uchida, Rikky Wenang Purbojati, Anthony Wong, Kavita K Kushwaha, Alexander Putra, Nicolas E. Gaultier, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Ana Carolina Martins Junqueira, Stephan C. Schuster. Complete Genome of Citricoccus sp. Strain SGAir0253 Isolated from Indoor Air in Singapore. Microbiology Resource Announcements. 2019 September 12; 8(37). Anjali Bansal Gupta, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexander Putra, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome Sequence of Streptomyces sp. strain SGAir0924, an Actinobacterium Isolated from Outdoor Air of Singapore. Microbiology Resource Announcements. 2019 August 29; 8(35). Anthony Wong, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N.I. Houghton, Caroline Chénard, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Nikappil S. Deepa, Alexander Putra, Nicolas E. Gaultier, Balakrishnan N.V. Premkrishnan, Vineeth Kodengil Vettath, Cassie E. Heinle, Daniela I. Drautz-Moses, and Stephan C. Schuster. Complete Genome Sequence of Pseudomonas sp. Strain SGAir0191 Isolated from Tropical Air Collected in Singapore. Microbiology Resource Announcements. 2019 August 22; 8(34). Namrata Kalsi, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Megan E. Clare, Kavita K Kushwaha, Deepa Panicker, Alexander Putra, Nicolas E. Gaultier, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome of Microbacterium sp SGAir0570 Isolated from Tropical Air Collected in Singapore. Microbiology Resource Announcements. 2019 August 22; 8(34). Rikky Wenang Purbojati, Daniela I. Drautz-Moses, Akira Uchida, Anthony Wong, Megan E. Clare, Kavita K Kushwaha, Alexander Putra, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Ngu War Aung, Vineeth Kodengil Vettath, Ana Carolina Martins Junqueira, Stephan C. Schuster. Complete Genome Sequence of Enterococcus faecalis Strain SGAir0397 Isolated from Tropical Air in Singapore. Microbiology Resource Announcements. 2019 August 15; 8(33). Phu Pwint Thin Hlaing, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexandra Putra, Carmon Kee, Nicolas E. Gaultier, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Serene B.Y. Lim, Vineeth Kodengil Vettah, Daniela I. Drautz- Moses, Stephan C. Schuster. Complete Genome Sequence of Brachybacterium rhamnosum strain Page 5 of 9

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SGAir0954 Isolated from Singapore Air. Microbiology Resource Announcements. 2019 August 8; 8(32).

Kenny J. X. Lau, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Nicolas E. Gaultier, Cassie E. Heinle, Balakrishnan N. V. Premkrishnan, Vineeth Kodengil Vettah, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome Sequence of Agrococcus sp. SGAir0287 Isolated from Tropical Air collected in Singapore. Microbiology Resource Announcements. 2019 August 8; 8(32).

Nicolas E. Gaultier, Ana Carolina M. Junqueira, Akira Uchida, Rikky W. Purbojati, James N. I. Houghton, Caroline Chénard, Anthony Wong, Sandra Kolundžija, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexander Putra, Carmon Kee, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Serene B.Y. Lim, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Stephan C. Schuster. Complete Genome of Nissabacter sp. Type Strain SGAir0207 Isolated from Singapore Air Samples. Microbiology Resource Announcements. 2019 August 8; 8(32). Rikky Wenang Purbojati, Daniela I. Drautz-Moses, Akira Uchida, Anthony Wong, Megan E. Clare, Kavita K Kushwaha, Alexander Putra, Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Ngu War Aung, Vineeth Kodengil Vettath, Ana Carolina Martins Junqueira, Stephan C. Schuster. Complete Genome Sequence of Brevundimonas sp. Strain SGAir0440 Isolated from Indoor Air in Singapore. Microbiology Resource Announcements. 2019 August 1; 8(31). Luhung I, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Premkrishnan BNV, Heinle CE, Vettath VK, Drautz-Moses DI, Schuster SC. Genome Sequence of Pantoea ananatis SGAir0210, Isolated from Outdoor Air in Singapore. Genome Announc. 2018 Jul 5;6(27). Lim SBY, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Premkrishnan BNV, Heinle CE, Vettath VK, Drautz-Moses DI, Schuster SC. Genome Sequence of Bacillus velezensis SGAir0473, Isolated from Tropical Air Collected in Singapore. Genome Announc. 2018 Jul 5;6(27).

Gaultier NE, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Clare ME, Kushwaha KK, Panicker D, Putra A, Kee C, Premkrishnan BNV, Heinle CE, Lim SBY, Vettath VK, Drautz-Moses DI, Schuster SC. Complete Genome Sequence of the Bacterium Serratia marcescens SGAir0764, Isolated from Singapore Air. Genome Announc. 2018 Jul 5;6(27).

Gaultier NE, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Kee C, Premkrishnan BNV, Heinle CE, Lim SBY, Vettath VK, Drautz-Moses DI, Schuster SC. Genome Sequence of Geobacillus thermoleovorans SGAir0734, Isolated from Singapore Air. Genome Announc. 2018 Jul 5;6(27). Kee C, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Premkrishnan BNV, Heinle CE, Vettath VK, Drautz-Moses DI, Schuster SC. Complete Genome Sequence of Acinetobacter schindleri SGAir0122 Isolated from Singapore Air. Genome Announc. 2018 Jun 28;6(26). Kee C, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Page 6 of 9

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Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Premkrishnan BNV, Heinle CE, Vettath VK, Drautz-Moses DI, Schuster SC. Complete Genome Sequence of Pseudomonas stutzeri Type Strain SGAir0442, Isolated from Singapore Air Samples. Genome Announc. 2018 May 31;6(22).

Premkrishnan BNV, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Wong A, Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Heinle CE, Vettath VK, Drautz-Moses DI, Schuster SC. Complete Genome Sequence of Staphylococcus haemolyticus Type Strain SGAir0252. Genome Announc. 2018 May 10;6(19). Vettath VK, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Drautz-Moses DI, Wong A, Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Heinle CE, Premkrishnan BNV, Schuster SC. Complete Genome Sequence of Acinetobacter indicus Type Strain SGAir0564 Isolated from Tropical Air Collected in Singapore. Genome Announc. 2018 May 3;6(18).

Heinle CE, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Drautz-Moses DI, Wong A, Kolundžija S, Clare ME, Kushwaha KK, Panicker D, Putra A, Gaultier NE, Premkrishnan BNV, Vettath VK, Schuster SC. Complete Genome Sequence of Lelliottia nimipressuralis Type Strain SGAir0187, Isolated from Tropical Air Collected in Singapore. Genome Announc. 2018 May 3;6(18).

Vettath VK, Junqueira ACM, Uchida A, Purbojati RW, Houghton JNI, Chénard C, Drautz-Moses DI, Wong A, Kolundžija S, Clare ME, Lau KJX, Gaultier NE, Heinle CE, Premkrishnan BNV, Gusareva ES, Acerbi E, Yang L, Schuster SC. Complete Genome Sequence of Bacillus altitudinis Type Strain SGAir0031 Isolated from Tropical Air Collected in Singapore. Genome Announcements. 2017 Nov 9;5(45).

Wong A, Lange D, Houle S, Valvano MA, Knirel Y., Dozois CM and Creuzenet C. Role of capsular modified heptose in the virulence of Campylobacter jejuni. Molecular Microbiology. 2015 June; 96(6): 1136-58.

Virginie Braun, Anthony Wong, Marija Landekic, Wan Jin Hong, Sergio Grinstein, and John H. Brumell. Sorting nexin 3 (SNX3) is a component of a tubular endosomal network induced by Salmonella and involved in maturation of the Salmonella-containing vacuole. Cell Microbiology. 2010 September 1; 12(9): 1352 – 67.

LIST OF PUBLICATIONS (SUBMITTED)

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Ngu War Aung, Elaine L. Oliveira, Anjali Bansal Gupta, Rikky W. Purbojati, Nicolas E. Gaultier, Anthony Wong, Deepa Panicker, Alexander Putra, Akira Uchida, Daniela I. Drautz-Moses and Stephan C. Schuster. The Complete Genome Sequence of Aspergillus fumigatus Strain SGAir0713 Isolated from Outdoor Air in Singapore.

Kenny J. X. Lau, Balakrishnan N.V. Premkrishnan, Anthony Wong, Tuang Yeow Poh, Akira Uchida, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Ana Carolina M. Junqueira, and Stephan C. Schuster. Structure vs. Chemistry: Alternate mechanisms for controlling leaf microbiomes.

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Balakrishnan N. V. Premkrishnan, Cassie E. Heinle, Akira Uchida, Rikky W. Purbojati, Kavita K. Kushwaha, Alexander Putra, Puramadathil Sasi Santhi, Benjamin WY Khoo, Anthony Wong, Vineeth Kodengil Vettath, Daniela I. Drautz -Moses, Ana Carolina M. Junqueira, Stephan C. Schuster. Comparative Genomics of Bacillus cereus strains Isolated from Indoor Air. LIST OF ABSTRACTS

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Anthony Wong. Imaging of Airborne Microorganisms. Air Microbiome Symposium. September 19 – 20 2019, Singapore.

Kenny J. X. Lau, Balakrishnan N.V. Premkrishnan, Anthony Wong, Tuang Yeow Poh, Akira Uchida, Vineeth Kodengil Vettath, Daniela I. Drautz-Moses, Ana Carolina M. Junqueira, and Stephan C. Schuster. Structure vs. Chemistry: Alternate mechanisms for controlling leaf microbiomes. International Society of Molecular Plant-Microbe Interaction, July 14 – 19 2019, Glasgow, Scotland.

Phu Pwint Thin Hlaing, Akira Uchida, Anthony Wong, Cassie Elizabeth Heinle, James Nicholas Ian Houghton, Stephan Schuster. Characterization of airborne microorganisms in Singapore using cultivation methods and DNA sequencing. Temasek Polytechnic Diploma in Biomedical Science Poster Presentation, January 2 2018, Singapore.

Eng Kai Lin Merrilyn, Anthony Wong, Akira Uchida. Isolation and identification of airborne microorganisms in Singapore using cultivation and molecular biology methods. Temasek Polytechnic Diploma in Biomedical Science Poster Presentation, January 2 2018, Singapore. Creuzenet C., Wong A., McCallum M., Barnawi H., Woodward L., Kubinec C., Lange D., Houle S., Shaw SD., Naismith. J, Dozois CM. and Shaw GS. Biochemical and functional analysis of modified heptoses from the capsule of Campylobacter jejuni. Canadian Glycomics Symposium, May 9-12 2017, Banff (Alberta, Canada).

Tan Kah Shean Christopher, Anthony Wong, Akira Uchida, Gusareva Elena, Stephan Schuster. Unravelling allergenic substances in the urban air environment of Singapore. Temasek Polytechnic Diploma in Biomedical Science Poster Presentation, January 3 2017, Singapore. McCallum M., Wong A., Lange D., Houle S., Shaw SD., Dozois CM., Shaw GS and Creuzenet C. Biochemical and functional analysis of modified heptoses from the capsule of Campylobacter jejuni. International Union of Microbiological Societies congresses, July 27-Aug 1 2014, Montreal

(Quebec, Canada).

Creuzenet C., McCallum M., Wong A., Lange D., Houle S., Shaw SD., Dozois CM., Shaw GS. Biochemical and functional analysis of modified heptoses from the capsule of Campylobacter jejuni. FASEB 2014, Microbial Glycobiology June 8-13 2014, Itasca (Illinois, USA). Anthony Wong, Takahiro Arakawa, Yusuke Oguchi, Masayoshi Itoh, and Sotaro Uemura. Multiple gene expression detection via a novel array system. RIKEN Centre for Life Science Technologies, July 3 – 4 2013, Kobe, Japan.

McCallum M., Wong A., Lange D, Shaw SD, Houle S., Dozois CM, Shaw GS, and Creuzenet C. Biochemical and functional analysis of modified heptoses from the capsule of Campylobacter Page 8 of 9

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jejuni. Canadian Campylobacter Workshop, Ottawa, ON. October 2012. Anthony Wong, Dirk Lange and Carole Creuzenet: Modified Heptose Synthesis in the Campylobacter jejuni Capsule and its Involvement in Virulence. Margaret Moffat Research Day, University of Western Ontario, London, ON. March 2011. Anthony Wong, Dirk Lange and Carole Creuzenet: Modified Heptose Synthesis in the Campylobacter jejuni Capsule and its Involvement in Virulence. Infection and Immunity Research Forum, University of Western Ontario, London, ON. November 2010. Anthony Wong, Dirk Lange and Carole Creuzenet: Modified Heptose Synthesis in the Campylobacter jejuni Capsule and its Involvement in Virulence. Margaret Moffat Research Day, University of Western Ontario, London, ON. March 2010. Anthony Wong, Virginie Braun and John Brumell: The Role and Functions of Sorting Nexins in S. Typhimurium Infections. The Hospital for Sick Children Poster Presentation Day, Toronto, ON. July 2007.

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LIST OF TECHNICAL SKILLS

Cell culture

Bacterial culture

Cell culture (human iPS, HeLa, HEK293, P. falciparum, RAW 264.7, Caco-2) Microscopy

Standard light microscopy (brightfield, darkfield, DIC, phase contrast) Light microscopy (widefield, scanning and spinning disc confocal, TIRF, N-SIM and N-STORM super resolution)

Scanning electron microscopy (procurement, sample preparation, imaging) Molecular Biology

Agarose gel electrophoresis

DNA extraction and purification

RNA extraction and purification

Polymerase chain reaction (PCR)

Quantitative PCR

Reverse transcription qPCR

Gene SOE-ing

Multiplex PCR

DNA Fluorescent in situ hybridization (FISH)

Transfection/siRNA knockdown

Basic bacterial transformation as well as novel transformation methods for C. jejuni (selective methylation followed by electroporation)

Protein Related

Gel chromatography

SDS-PAGE and subsequent visualization methods (Alcian Blue, Coomassie, Silver, etc) Western Blot

Immunohistochemistry

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