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Engineering Plant

Location:
Qadian, Punjab, India
Posted:
September 30, 2020

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Resume:

Saiqa

Problem solver and highly motivated skilled

biotechnology fresher.

Internship at Department of Biophysics, Panjab University, Chandigarh

Supervisor: Dr. Sarvnarinder kaur

Duration: January to June, 2020 6 Months

Title of internship: To determine the chemopreventive potential of hydro-ethanolic Murayya koenigii leaves extract (HEMKLE) against DMBA/TPA induced skin carcinogenesis in mice. Description: Skin cancer was induced by applying DMBA/TPA on the skin of mice, resulted in DNA mutations occurred in oncogenes and tumor suppressor genes. The observations of the present study clearly indicated that HEMKLE exerted remarkable chemopreventive effects against DMBA/TPA induced skin carcinogenesis. The retardation in the development of skin carcinogenesis upon HEMKLE supplementation was revealed through reduced histo-architectural alterations, reduced incidence as well as total number of tumors. It has also shown to modulate key processes like ROS generation, LPO levels and activity of antioxidant enzymes which are crucial in the initial stages of chemical carcinogenesis.

B.Tech Biotechnology

Punjab Technical University; (2016-2020)

Major subjects: Cell and molecular

biology, Microbiology, Immunology,

Genetic engineering, Biostatistics, Animal

tissue culture, and Bioprocess

engineering.

9.16 CGPA

Date of Birth

November 26, 1997

Address

H.No. 104/8, Ahmadiyya street,

Qadian, Teh. Batala, Distt.

Gurdaspur, Punjab, India.

Email

adgiw5@r.postjobfree.com

LinkedIn

https://www.linkedin.com/in/saiq

a-bajwa-a9466414b

Skype

saiqa.qadian2

Mobile No

+91-750*******

Hobbies

Reading, travelling, and singing

Languages

English and learning Deutsch

Educational event

management

President of two college

society

Personal information

Academic qualification

Work experience 8 Months

Extracurricular activities

Internship at Institute of Nano Science and Technology, Mohali

Supervisor: Dr. Manish Singh

Duration: May to July, 2018 2 months

Title of internship: Quantitative analysis of azo dye Eriochrome Black T (EBT) in industrial effluent and its in vitro toxicological studies.

Description: Determination of the quality of water samples

(from industrial effluent sites) by measuring Total dissolved solute (TDS) concentration, COD, and BOD and concentration of EBT in water sample was measured by UV-VIS

Spectrophotometry. MTT assay was performed to measure the cytotoxicity of EBT on NIH-3T3 cell lines, revealing that increase in dose of EBT resulted in increased cell toxicity as well as cell mortality.

Bacterial culturing techniques- Media preparation, stearilization, filtration, spreading, streaking, subculturing, bacterial isolation, preservation, physical and biochemical testing.

Downstream processing- DNA extraction, RNA extraction, centrifugation, protein extraction and purification (filtration, concentration, electrophoresis, chromatography, and dialysis)

Immunology- ELISA, immune-precipitation, immune- agglutination, and Dot Blot

Biochemistry- Qualitative and quantitative analysis of amino acids, protein, and carbohydrates.

In vitro studies- Good lab practices in BSL-II, maintenance of cell lines, sub-culturing, preservation, MTT assay, microscopy, lyophilization, and SEM.

Tumor studies in vivo- Induction of skin tumor, protectant administration, isolation of brain and liver, homogenate preparation, histology, biochemical estimations of ROS, LPO, GST, GPx, and LDH.

Plant tissue culture techniques- Micro-propagation and organogenesis.

Strong communication skills

Good presentation skills

Determined

Team leader

Friendly

Technical skills

Strengths



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