CHHAVI GUPTA

PROFILE

I am an enthusiastic and self-motivating person with extensive experience in Mycobacterium tuberculosis diagnostics and genotyping. I am highly motivated to learn and eager to contribute to team success through hard work, attention to detail and excellent organizational skills. My real strength is my passion, teamwork, and never-ending enthusiasm.

Contact information:

134 Marburth Ave, Baltimore,

MD 21286

Phone: 443-***-****

Email: adg1am@r.postjobfree.com

SKILLS

Microbiology

Nucleic acid (DNA and RNA) extraction

PCR (RT-PCR, DD-PCR,

Real time PCR)

Media preparation

Handling of clinical specimens and blood specimens

Handling of infectious micro-organisms (Mycobacterium tuberculosis, COVID19)

Culture of pathogenic bacteria (Mycobacterium tuberculosis) and nonpathogenic micro- organisms

Drug susceptibility testing, MIC and MBC determination

Biosafety Level 2 (BSL-2) & Biosafety Level 3 (BSL-3) experience

Mycobacteriophage experience

Cell culture

Sanger's sequencing

Cloning and transformation

Genotyping techniques: Spoligotyping, MIRU-VNTR

Primers and probes design

DNA and protein sequence analysis using various software's

Writing and analysis of laboratory procedures (SOPs), manuscripts

Technical writing

EDUCATION

2006-02 TO 2012-09: PHD (MICROBIOLOGY)

All India Institute of Medical Sciences - New Delhi, India

2002-07 TO 2004-12: MASTER OF SCIENCE (MICROBIOLOGY)

Dr Bhimrao Ambedkar University - Agra, India

1997-07 TO 2000-06: BACHELOR OF SCIENCE (BIOLOGY)

Dr Bhimrao Ambedkar University - Agra, India

WORK EXPERIENCE

MOLECULAR SCIENTIST (7/21/20 TO 8/31/20: 6-WEEK CONTRACT POSITION)

Real Diagnostics, Reisterstown, Baltimore, MD

Project was "Diagnosis of COVID19 by using real time PCR". Experience earned during this time is handling and accessing of COVID19 clinical specimens and to handle high throughput machine like:

Kingfisher Flex for RNA extraction

Integra and Agilent-bravo workstation for real time PCR

QuantStudio12K for real time PCR analysis

Post Doctorate Fellow (2017-09 to 2019-06)

Johns Hopkins University, Baltimore, United States

Project was "Genomic analysis of non-tuberculous mycobacteria". MIC (Minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) was performed on nontuberculous mycobacteria using liquid media, solid media and sensititre plates. Combining different approaches helps to validate results. DNA extraction and quantification were done, and samples sent to do whole genome sequencing. Additionally, other experiments were also carried like:

Checkerboard assay to find out synergy between drugs (antibiotics).

Mutant generations

MIC and MBC determination

Drug susceptibility testing,

Handling of infectious pathogens in BSL-2 and BSL-3 laboratories.

Post Doctorate Fellow (2015-07 to 2016-05)

Johns Hopkins University, Baltimore, United States

Project was "Phage therapy to treat M. tuberculosis". This was very interesting project where synthetic Myco- bacteriophage was designed that could be able to survive in host but should be able to kill bug (MTB). Synthetic chunks of D29 (myco-bacteriophage) was designed and transformed in yeast and further re transformed in E. coli. Various steps were taken to accomplish goals, and some are listed below:

Mycobacteriophage amplification and characterization

Myco-bacteriophage DNA isolation

Cloning and transformation.

Primers design

Post Doctorate Fellow (2015-07 to 2016-05)

University of Maryland, Baltimore, Maryland

Project was "Identification of small noncoding RNA as biomarkers for lung cancer patients using molecular approaches". Focus of this project was to identify small non-coding pi-RNA as biomarker in lung cancer patients. Clinical specimens were collected and stored as per guidelines. Major duties involve achieving goals were:

Isolation of PBMCs from blood samples

Cell culture

RNA isolation

PCR: DD (droplet digital)-PCR, Real time PCR, RT-PCR

Gene knockdown

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