Surinder Kaur, MSc.

Lead Research Specialist

Department of Microbiology and Immunology

Emory Vaccine Center,

Yerkes National Primate Research Center,

*** ******** ****, ******* *****.

Atlanta, Georgia 30329 USA

Tel: 770-***-****,

e-mail: addmrn@r.postjobfree.com

CURRICULUM VITAE

CAREER OBJECTIVE

Seeking a challenging position in biological sciences in an academic/research setting. My interests in basic sciences encourage me to seek out an opportunity in any research firm. With the basic training, which I have already gained in learning the principles of microbiology, virology and immunology through these years, I would like to broaden my expertise and specialization in related research. I am committed to my work and always ready to put in extra time to achieve the goals. I am eager to learn new things and apply the same to fulfill the project’s requirement, which would eventually advance my knowledge. I am confident that I can make a strong contribution to any kind of research projects related to my focused field.

EDUCATION

2009 MS in Microbiology, Georgia State University. Atlanta, Georgia, USA.

Dissertation:

Major Professor: Dr. Rithu Aneja, Ph.D.

2006 B.Ed. Science, Punjab University, Punjab, India.

2003 BSc Medical Science, Punjab University, Punjab, India.

RESEARCH EXPERIENCE

I have worked as a Lead- research specialist in the Department of Microbiology and Immunology, Emory Vaccine Center over 8 years and investigated the immune response against various microbial pathogens such as (Influenza, HIV, SIV, Tuleremia, Malaria).

I have proven ability to perform highly competitive research projects, which are aimed for detecting immune response (both humoral and cell mediated immunity) against vaccine antigens such as (Tularemia, Tuberculosis and Malaria).

Well trained in multicolor flow-cytometry (15 colors) to detect immune cells such as T cells, B cells, Dendritic cells, macrophages and monocytes after vaccination and following infection.

Well trained in Elispot assay and ELISA assay to detect immune response against T or B cells.

Trained in performing experiments that determines organic compounds in water, air and soil samples using gas chromatography (GC), mass spectrometry.

Tissue culture of sugarcane and aloe vera, prepared various growth media using aseptic techniques.

Worked as a teaching assistant and taught lab molecular techniques, cell biology, Gram staining, isolation and identification of microorganisms.

AWARDS AND HONORS

2014 Best publication award for the manuscript entitled “Role of Intestinal Mucosal Integrity in HIV Transmission to Infants Through Breast-feeding: The BAN Study. Journal of Infectious Disease. CDC.

MENTORING AND TRAINING

2011- current Provided basic training for graduate/undergraduate/post doctoral students in Emory Vaccine Center:

: Blood processing using Fycol, CPT tubes

: To perform multicolor flow cytometry assays; Staining, acquisition and analysis.

: Elispot Assays for ASCs, Memory B cells, Plasma cells, Cytokines.

MAJOR RESEARCH INTERESTS

Microbial Immunology

- Adaptive T cell immunity in lymphoid and non-lymphoid tissues

- Blood specific regulation of cytotoxic effector functions

- Regulatory T cells in Blood

- Chemokine/receptor interactions in lymphoid and non-lymphoid tissues.

Viral Pathogenesis

- Identification of Immune exhaustion markers for HCV, HIV, CMV, EBV, FLU infection.

- Immune activation/Inhibitory receptors and HIV/SIV infection.

PROFESSIONAL MEMBERSHIPS

2011- present Member, Emory Center for AIDS Research (CFAR).

TECHNICAL EXPERTISE

Immunology: PBMC Isolation: Freezing/Thawing cells for various Immunology Assays

Isolation and quantitation of T cells, B cells, Dendritic cells and Macrophages- phenotypic analysis- Design and Phenoypic and functional haracterization of vaccine antigens

Cytokine and Antigen ELISAs (LAL, CD64L, IL-6, IL-17 etc),

Flow cytometry: Multicolor flow cytometry Assays- 16 color (Phenotype/Functional assays)

CD8/NK cells: Cytotoxicity assays, T/B cell cytokine assays

Cell sorting: Sorting of ASC from total PBMCs using FACS Aria.

T, B, Dendritic cells and Macrophages Phenotypic analysis

Human Dendritic Cell Activation assays,

Flow cytometry training and Analysis training using FACS

Molecular Biology: Gene cloning Techniques, Transgene expression studies

RNA/DNA extraction using extraction kits.

Cell Biology: Cell Culture techniques, Human Monocyte to MDM differentiation

Protein expression and Quantitation

Cell proliferation and Viability assays

Statistics: PRISM software, Excel.

Virology: Tetramer Titration Analysis. (CMV, EBV, HCV, HIV and TB antigens).

Peptides

Animal models: Rhesus,

Good hands on Animal Necropsies and tissues processing etc.

Non-human primate models of HIV/AIDS. (Rhesus, African Green Monkeys, Pigtails)

PUBLICATIONS

1.Role of Intestinal Mucosal Integrity in HIV Transmission to Infants Through Breast-feeding: The BAN Study. J Infect Dis. 2013 Aug 15;208(4):653-61. Athena P. Kourtis, Chris C. Ibegbu, Jeffrey Wiener, Caroline C. King, Gerald Tegha, Deborah Kamwendo, Jacob Kumwenda, Surinder P. Kaur, Valerie Flax, Sascha Ellington, Zebrone Kacheche, Dumbani Kayira, Charles Chasela.

2.Induction of human plasmablasts during infection with antibiotic-resistant nosocomial bacteria". J Antimicrob Chemother. 2014 Jul;69(7):1830-3. Victor Band, Chris Ibegbu, Surinder P Kaur, Stephanie Cagle, Ronald Trible, Crystal Jones, Yun Wang, Colleen Kraft, Susan Ray, Jens Wrammert, and David Weiss.

3.Skin immunization of non-human primates with influenza subunit vaccine. Ioanna Skountzou, Chris Ibegbu, Priya Kalluri, Devin McAllister, E.Stein Esser, Misha Taherbha, Elena Vassilieva, Chris Edens, Joanna Pulit-Penaloza, Surinder P. Kaur, Dimitrios Koutsonanos, Mark R. Prausnitz and Richard Compans, (Manuscript in preparation).

4.Differences in expression of gut-homing receptors on CD4+ T cells in black and white HIV-negative men who have sex with men. AIDS. 2016 May 15;30(8):1305-8. Kelley CF, Lai L, Ibegbu C, Rosenberg ES, Kaur S, Patel K, Mulligan MJ, Marconi VC, Sullivan PS, Amara RR.

RESEARCH ABSTRACTS (Poster & oral communications).

1.Emory CFAR Annual Symposium, 2013 – Center for AIDS research Immunology and Cytometry core at Emory Vaccine Center. (Invited presentation).

2.Role of intestinal mucosal integrity in HIV transmission to infants through breastfeeding: the breastfeeding, Antiretrovirals, and Nutrition Study. Presented in CROI (Conference on Retroviruses and Opportunistic Infections) 2013, Atlanta, Georgia.

3.Homeostasis of CD4+ T-cells in non-human primates. Presented in CROI (Conference on Retroviruses and Opportunistic Infections) 2013, Atlanta, Georgia.

4.Skin immunization of non-human primates with influenza subunit vaccine. Presented in Skin Vaccination Summit 2013, Seattle, Washington, USA

5.ROLE OF REGULATORY T CELLS IN MOTHER TO CHILD TRANSMISSION OF HIV" (ID 2259), a poster presentationin CROI (Retroviruses and Opportunistic Infections) (Peter A. Kessler1, Surinder P. Kaur1, Chris C. Ibegbu1

References: Up on request.

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