Caleb Ruiz-Jiménez, Ph.D.
Highly motivated and independent scientist. I hope to join a team where I can make an impact with my experience in immunology, molecular biology and protein purification techniques that will help develop new technologies to fulfill unmet biotechnological needs. 10+ years in interdisciplinary life sciences experience. SKILLS
• Bilingual (Spanish, English)
• PDX model
• Cell Culture (Bacterial, Mammalian)
• Blood processing for stem CD34+ cells isolations
• Protein Expression and Purification
• Western Blotting
• ELISA Assays
• Immunohistochemical Staining, Confocal Microscopy
• Bacterial Growth and Expression Assays
• Molecular Cloning
• IVIS Imaging CT System
• In vitro Assays (TLRs stimulation, Co-culture- DCs/Tcells)
(Transcription and transfection)
• Flow cytometry
• Affinity chromatography and FPLC
LOMA LINDA UNIVERSITY Loma Linda, CA
Postdoctoral Fellow July 2019 – Present
• Develop and optimize cancer cell cultures and stroma cell cultures transfected with protein of interest.
• Develop and maintain patient-derived xenograft (PDX) animal model of leukemia tumors.
• Test protein of interest in PDX mice model as a leukemia treatment.
• Develop experiments to study drug candidate stability.
• Follow up experiments with flow cytometry and with IVIS Spectrum CT system
• Isolating stem cells from cord blood and bone marrow samples from donors (human patients from hospital).
• Assisted senior staff in writing SOPs for grant proposals.
• Mentoring graduate and undergrad students on immunohistochemical and flow cytometry experiments.
• Supervise lab staff.
• Participated as a professor in an immunology graduate course. UNIVERSITY OF PUERTO RICO, MEDICAL SCIENCES CAMPUS San Juan, PR Graduate Research Assistant August 2012 – June 2019
• Recombinant Protein expression and purification.
• Native Protein Purification.
• Endotoxin Removal Process.
• In vitro experiments to study inflammation biomarkers with flow cytometry, ELISA, qPCR, WB and others techniques.
• Studying inflammatory and anti-inflammatory effects of different drugs/protein on the immune system.
• Animal model for septic shock.
• Mentoring undergrad students.
NATIONAL UNIVERSITY OF CORDOBA Córdoba, Argentina
Graduate Internship Researcher April – August 2016
• Differentiate dendritic cells from murine bone marrow.
• Study biologic drug effect on dendritic cells.
• Develop a co-culture with dendritic cells and splenocytes to study anti-inflammatory drug effects.
• Study inflammatory and apoptosis biomarkers on dendritic cells. UNIVERSITY OF PUERTO RICO, MEDICAL SCIENCES CAMPUS San Juan, PR Undergraduate Research Assistant May 2011 – July 2012
• Recombinant Protein expression and purification.
• iRNA assays.
• Design ELISA assays for diagnostic purpose.
• Design and test vaccine with recombinant protein in animal models. EDUCATION
• Loma Linda University, Loma Linda, California, 2019-2020 Postdoctoral Fellow in Cancer Research
• University of Puerto Rico, Medical Sciences Campus, San Juan, PR, 2012-2019: Ph.D. in Microbiology & Medical Immunology, Area of Specialization: Immunology, GPA: 3.66, Magna Cum Laude
• University of Puerto Rico, Bayamon, PR, 2004-2009 Bachelor in Natural Sciences, Major in Human Biology. AWARDS and HONORS
• Minorities Travel Award – American Association of Immunologists (AAI) 2020
• Trainee Abstract Award – American Association of Immunologists (AAI) 2020
• Minorities Travel Award- American Association of Cancer Research (AACR) 2020
• NIH Diversity Supplement for Postdoctoral Position- 2019- 2021
• Travel Award for Oral Presentation at Cancun Mexico, American Society of Parasitologists 2018
• Immunology Internship at National University of Cordoba, Argentina 2016
• MBRS-RISE Program Fellowship Funding 2014-2019
• Honor Student, UPR Medical Sciences Campus, 2013-2019
• Outstanding Grade Point Average, UPR Medical Sciences Campus, 2014-2019
• Outstanding in Research, UPR Medical Sciences Campus, 2015-2019
• Florida International University Travel Award 2012 for Biomedical and Comparative Immunology Symposium for Poster Presentation
• Smart Grant 2007-2009
• President of Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter, San Juan, PR. 2017-2018
• Medicine Student Council Member, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2014- 2017
• Representative of School of Medicine at General Student Council, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2014-2017
• Ph.D. Student Representative at Graduate Committee of Biomedical Sciences Associate Deanship, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2015- 2016
• President of General Student Council of University of Puerto Rico Medical Sciences Campus, San Juan, PR, 2015- 2016
• Senator at Academic Senate of University of Puerto Rico, Medical Sciences Campus, San Juan, PR, 2015 to 2016
• Student Representative at Graduate Committee Department of Microbiology, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2013- 2015
• Secretary, Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter. San Juan, PR. 2014- 2015
• Public Relations Specialist, Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter. San Juan, PR. 2012- 2015
• Ruiz-Jiménez C*, Ramos-Benítez MJ*, Aguayo V, Espino AM. 2017. Recombinant Fasciola hepatica fatty acid binding protein suppresses toll-like receptor stimulation in response to multiple bacterial ligands. Scientific Report. 2017 Jul 14;7(1):5455. Doi: 10.1038/s41598-017-05735-w. PMID: 28710478 *First author equal contribution.
• Ramos-Benitez, M. J., Ruiz-Jimenez, C., Rosado-Franco, J. J., Ramos-Pérez, W. D., Mendez, L. B., Osuna, A., & Espino, A. M. (2018). Fh15 Blocks the Lipopolysaccharide-Induced Cytokine Storm While Modulating Peritoneal Macrophage Migration and CD38 Expression within Spleen Macrophages in a Mouse Model of Septic Shock. mSphere, 3(6), e00548- 18. Doi:10.1128/mSphere.00548-18
• Aguayo, V., Valdés Fernandez, B. N., Rodríguez-Valentín, M., Ruiz-Jiménez, C., Ramos-Benítez, M. J., Méndez, L. B., & Espino, A. M. (2019). Fasciola hepatica GST downregulates NF-κB pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model. Scientific reports, 9(1), 2275. Doi:10.1038/s41598-018-37652-x
• Ramos-Benítez MJ, Lopez-Cruz LM, Aguayo V, Ruiz-Jimenez C., & Espino AM. Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2. Mol Biol Rep. 2018 Oct;45(5):1551-1556. Doi: 10.1007/s11033-018-4251-3.
• Cabán-Hernández, K., Gaudier, J. F., Ruiz-Jiménez, C., & Espino, A. M. (2014). Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis. Journal of clinical microbiology, 52(3), 766–772. Doi:10.1128/JCM.02875-13