Caleb Ruiz-Jiménez, Ph.D.

****-* ********** ****** • Durham, NC 27705 • 787-***-**** • adddqg@r.postjobfree.com SUMMARY OF QUALIFICATIONS

Highly motivated and independent scientist. I hope to join a team where I can make an impact with my experience in immunology, molecular biology and protein purification techniques that will help develop new technologies to fulfill unmet biotechnological needs. 10+ years in interdisciplinary life sciences experience. SKILLS

• Bilingual (Spanish, English)

• PDX model

• Cell Culture (Bacterial, Mammalian)

• Blood processing for stem CD34+ cells isolations

• Protein Expression and Purification

• Western Blotting

• ELISA Assays

• Immunohistochemical Staining, Confocal Microscopy

• Bacterial Growth and Expression Assays

• Molecular Cloning

• IVIS Imaging CT System

• In vitro Assays (TLRs stimulation, Co-culture- DCs/Tcells)

(Transcription and transfection)

• Flow cytometry

• Affinity chromatography and FPLC

• qPCR

WORK EXPERIENCE

LOMA LINDA UNIVERSITY Loma Linda, CA

Postdoctoral Fellow July 2019 – Present

• Develop and optimize cancer cell cultures and stroma cell cultures transfected with protein of interest.

• Develop and maintain patient-derived xenograft (PDX) animal model of leukemia tumors.

• Test protein of interest in PDX mice model as a leukemia treatment.

• Develop experiments to study drug candidate stability.

• Follow up experiments with flow cytometry and with IVIS Spectrum CT system

• Isolating stem cells from cord blood and bone marrow samples from donors (human patients from hospital).

• Assisted senior staff in writing SOPs for grant proposals.

• Mentoring graduate and undergrad students on immunohistochemical and flow cytometry experiments.

• Supervise lab staff.

• Participated as a professor in an immunology graduate course. UNIVERSITY OF PUERTO RICO, MEDICAL SCIENCES CAMPUS San Juan, PR Graduate Research Assistant August 2012 – June 2019

• Recombinant Protein expression and purification.

• Native Protein Purification.

• Endotoxin Removal Process.

• In vitro experiments to study inflammation biomarkers with flow cytometry, ELISA, qPCR, WB and others techniques.

• Studying inflammatory and anti-inflammatory effects of different drugs/protein on the immune system.

• Animal model for septic shock.

• Mentoring undergrad students.

NATIONAL UNIVERSITY OF CORDOBA Córdoba, Argentina

Graduate Internship Researcher April – August 2016

• Differentiate dendritic cells from murine bone marrow.

• Study biologic drug effect on dendritic cells.

• Develop a co-culture with dendritic cells and splenocytes to study anti-inflammatory drug effects.

• Study inflammatory and apoptosis biomarkers on dendritic cells. UNIVERSITY OF PUERTO RICO, MEDICAL SCIENCES CAMPUS San Juan, PR Undergraduate Research Assistant May 2011 – July 2012

• Recombinant Protein expression and purification.

• iRNA assays.

• Design ELISA assays for diagnostic purpose.

• Design and test vaccine with recombinant protein in animal models. EDUCATION

• Loma Linda University, Loma Linda, California, 2019-2020 Postdoctoral Fellow in Cancer Research

• University of Puerto Rico, Medical Sciences Campus, San Juan, PR, 2012-2019: Ph.D. in Microbiology & Medical Immunology, Area of Specialization: Immunology, GPA: 3.66, Magna Cum Laude

• University of Puerto Rico, Bayamon, PR, 2004-2009 Bachelor in Natural Sciences, Major in Human Biology. AWARDS and HONORS

• Minorities Travel Award – American Association of Immunologists (AAI) 2020

• Trainee Abstract Award – American Association of Immunologists (AAI) 2020

• Minorities Travel Award- American Association of Cancer Research (AACR) 2020

• NIH Diversity Supplement for Postdoctoral Position- 2019- 2021

• Travel Award for Oral Presentation at Cancun Mexico, American Society of Parasitologists 2018

• Immunology Internship at National University of Cordoba, Argentina 2016

• MBRS-RISE Program Fellowship Funding 2014-2019

• Honor Student, UPR Medical Sciences Campus, 2013-2019

• Outstanding Grade Point Average, UPR Medical Sciences Campus, 2014-2019

• Outstanding in Research, UPR Medical Sciences Campus, 2015-2019

• Florida International University Travel Award 2012 for Biomedical and Comparative Immunology Symposium for Poster Presentation

• Smart Grant 2007-2009

LEADERSHIP POSITIONS:

• President of Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter, San Juan, PR. 2017-2018

• Medicine Student Council Member, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2014- 2017

• Representative of School of Medicine at General Student Council, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2014-2017

• Ph.D. Student Representative at Graduate Committee of Biomedical Sciences Associate Deanship, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2015- 2016

• President of General Student Council of University of Puerto Rico Medical Sciences Campus, San Juan, PR, 2015- 2016

• Senator at Academic Senate of University of Puerto Rico, Medical Sciences Campus, San Juan, PR, 2015 to 2016

• Student Representative at Graduate Committee Department of Microbiology, University of Puerto Rico, Medical Sciences Campus, San Juan, PR. 2013- 2015

• Secretary, Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter. San Juan, PR. 2014- 2015

• Public Relations Specialist, Association of Microbiology of Puerto Rico, Medical Sciences Campus Chapter. San Juan, PR. 2012- 2015

SELECTED PUBLICATIONS

• Ruiz-Jiménez C*, Ramos-Benítez MJ*, Aguayo V, Espino AM. 2017. Recombinant Fasciola hepatica fatty acid binding protein suppresses toll-like receptor stimulation in response to multiple bacterial ligands. Scientific Report. 2017 Jul 14;7(1):5455. Doi: 10.1038/s41598-017-05735-w. PMID: 28710478 *First author equal contribution.

• Ramos-Benitez, M. J., Ruiz-Jimenez, C., Rosado-Franco, J. J., Ramos-Pérez, W. D., Mendez, L. B., Osuna, A., & Espino, A. M. (2018). Fh15 Blocks the Lipopolysaccharide-Induced Cytokine Storm While Modulating Peritoneal Macrophage Migration and CD38 Expression within Spleen Macrophages in a Mouse Model of Septic Shock. mSphere, 3(6), e00548- 18. Doi:10.1128/mSphere.00548-18

• Aguayo, V., Valdés Fernandez, B. N., Rodríguez-Valentín, M., Ruiz-Jiménez, C., Ramos-Benítez, M. J., Méndez, L. B., & Espino, A. M. (2019). Fasciola hepatica GST downregulates NF-κB pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model. Scientific reports, 9(1), 2275. Doi:10.1038/s41598-018-37652-x

• Ramos-Benítez MJ, Lopez-Cruz LM, Aguayo V, Ruiz-Jimenez C., & Espino AM. Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2. Mol Biol Rep. 2018 Oct;45(5):1551-1556. Doi: 10.1007/s11033-018-4251-3.

• Cabán-Hernández, K., Gaudier, J. F., Ruiz-Jiménez, C., & Espino, A. M. (2014). Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis. Journal of clinical microbiology, 52(3), 766–772. Doi:10.1128/JCM.02875-13

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