Sravya Challa

Las Vegas, NV

adbfd2@r.postjobfree.com

+1-903-***-****

• Analytical chemist with 5+ years of laboratory experience in raw material and finished product analysis using various analytical instruments and techniques.

• 5+ years of Mass spectrometry, UPLC, LC/MS/MS, GC/MS, GC-Headspace experience.

• Exceptional problem solving and decision-making capacity, ability to work in high pressure environment and excellent organizational skills.

• Practice in Spectrometric and chromatographic techniques for characterization of various peptides, polymers and complex chemical molecules.

• Skilled at learning and applying new instrumental techniques, programming and technical skills.

• 7+ years of experience using MS Office- MS Word for documentation, Microsoft Excel for calculations, analysis and data management and MS Power point for presentations.

• Strong documentation skills, excellent English communication and interpersonal skills, with the ability to break down complex terms and concepts into clear business language. Willing to relocate: Anywhere

Authorized to work in the US for any employer

Work Experience

Sr. Chemist/QA/QC manager

MM Lab, Inc - Las Vegas, NV

September 2018 to Present

• Validated various instruments including Schimadzu LCMS- 8050, GC/MS (QP2010 series), Schimadzu Nexera LC-2040C series, GC-2010 headspace and Perkin Elmer ICP-MS.

• Performed the complete Analytical method development process for LC-MS-MS assays, GCMS assays, GC-FID and HPLC assays, including validations and routine assays.

• Performed complete validation for accuracy, linearity, LOD, LOQ, matrix effect, precision RSD, selectivity, robustness and stability.

• Extensive evaluation of precision and robustness.

• Worked on full validation protocols and reports. .

• Constructed the comprehensive reference tandem LC/MS/MS library of about 35 analytes and GCMS libraries.

• Established, manage and monitor the Quality control and Quality assurance systems and the integral standard operating procedures and other quality documents to provide high-quality products and services of the organization.

• Performed the complete Analytical method development process for LC-MS-MS assays, GCMS assays, GC-FID and HPLC assays, including

Complete validation for accuracy, linearity, LOD, LOQ, matrix effect, precision RSD, selectivity, robustness and stability.

Extensive evaluation of precision and robustness.

• Worked on full validation protocols and reports.

• Played a vital role in the process of ISO17025 accreditation of the organization. Analytical Chemist

MM Lab, Inc - Las Vegas, NV

February 2018 to September 2018

• Performed the qualitative and quantitative assays employing Schimadzu LCMS- 8050 and GC/ MS (QP2010 series) employing MRM scan type in positive electrospray ionization (ESI) mode, with LabSolutions software.

• Operate, calibrate, troubleshoot the Analytical chemistry equipment such as LCMS, UV-Vis spectrometer, HPLC, GCMS and GC-FID.

Chemist

American Specialty lab - Las Vegas, NV

March 2017 to January 2018

• Performed the chemical tests and assays.

• Quantitate the analytes (75 analytes) by GC/MS (QP2010 series) and gradient high-performance liquid chromatography tandem mass spectrometry (Shimadzu HPLC and API 3000 triple LC/MS/MS system) employing MRM scan type in positive electrospray ionization (ESI) mode.

• Perform the GC/MS maintenance and troubleshooting (checking gas levels (above 100 psi), check septa and glass inserts, Broad and misplaced N2 peak: Indicative of N2 leak into the system requires locating and fixing etc.)

• Operate, calibrate, troubleshoot and perform HPLC - LC/MS/MS maintenance

• Acquired data using the AB Sciex Analyst software and quantified with the MultiQuant 2.1 software.

Configure the hardware and the security settings, tune and calibrate the mass spectrometer.

Built the gradient acquisition method and the quantitation method.

• Validated the LC/MS/MS and GC/MS assay for linearity, limit of detection (LOD), lowest limit of quantitation (LLOQ), precision, accuracy, interference and matrix effect, sample stability, carryover and correlation.

• Faced and overcame the LCMS challenges during the testing, such as sudden retention time shift, variable retention times, high column pressure, increased baseline noise, unusual noises from the instrument due to increased friction in the turbo pump due to its failure etc.

• Maintained laboratory notebooks and documented the work done. Clinical SAS trainee

Blue cube IT

September 2016 to March 2017

• Utilize SAS programming skills within protocol team and perform all programming required for clinical trial analysis and reporting.

• Perform Quality control on final reports.

• Develop SAS coding and table templates for preparing, processing and analyzing clinical data.

• Establish monitoring of data transfers for ongoing trials to identify study conduct or Data Quality issues.

Graduate Teaching Assistant

Texas A&M University - Commerce, TX

May 2014 to June 2016

• Taught undergraduate chemistry majors on instrumentation such as HPLC/UPLC, GC, TGA, DSC, UV- Vis spectrophotometer, dissolution equipment and other analytical instruments and Wet chemistry techniques.

• Taught about automated pipettes, centrifuge, dishwasher, analytical balance, lab coat, gloves, gas cylinders, vacuum extraction apparatus, evaporator and pneumatic crimper.

• Prepared chemical solutions, reagents and buffers in the desired molarities for use in gravimetric, colorimetric and wet chemistry techniques.

• Performed Quantitative analysis using Gravimetric, Volumetric and Colorimetry methods, Dissolution, Flame Atomic Absorption, Polarimetry, Loss on Drying, Viscosity, Residue on Ignition and Heavy Metal Content.

• Tutoring students to understand key concepts and experiments in chemistry labs.

• Grade lab reports and meet with students during office hours and clarify their doubts.

• Led weekly group discussions on concept generation and problem-solving techniques. Research assistant

Texas A&M University - Commerce, TX

January 2014 to June 2016

• Performed research in analytical chemistry, executed assigned experiments that support development activities and project goals, acquired strong background in HPLC and GC/Headspace, MS/ MS, LC/MS, GC/MS and GPC.

• Operate, tune and calibrate, troubleshoot and perform Mass Spectrometer maintenance

Set the mass spectrometer parameters such as source temperature, Capillary and sample cone voltages, pressure of nebulizer and curtain gas, flow rate etc.

Removing and cleaning the sample cone and extraction cone.

Calibrate the Mass Spectrometer using Polyethylene Glycol (PEG) + Ammonium acetate.

• Prepared chemical solutions, reagents and buffers for use in the spectrometric analysis of peptides and maintained safe environment.

• Worked in a team of chemical engineers, analytical chemists, materials scientists and biologists.

• Accurately analyzed data from test results, prepared technical reports for publications and presentations, documented laboratory work, maintained and organized lab reports and created required standard operating procedures (SOP).

• Developed analytical methods and performed high quality analytical testing in accordance with USP/NF/EP/JP/FCC and maintained physical and electronic reference standard inventory and sample handling to 3rd party contract laboratories.

• Performed shipping of packages and electronic orderings of supplies. Research project:

• "Analysis of Cu(II) and Zn(II) binding properties of peptides as a function of varying pH and metal equivalents employing ion mobility mass spectrometric studies"

• Performed Investigation of Cu (I) and Zn (II) binding affinity of small peptides which helps to understand the mechanism associated with forming amyloid-b complexes related to the Alzheimer's disease.

• Synthesized and purified a series of peptides based on the structure of mb-OB3b replacing the two enethiol-oxazolone groups with the alternative metal binding 2His-2Cys motif.

• Worked on Waters Synapt high definition mass Spectrometer (HDMS) combining ESI (electrospray ionization source), quadrupole, ion mobility cell and orthogonal time of flight mass analyzer in both positive and negative ionization modes, employed in the study of metal binding of peptides.

• Prepared peptide stock solution, required dilutions, metal solutions (ZnCl2) and the pH was adjusted with the addition of acetic acid and Ammonium hydroxide.

• Employed different liquid chromatographic techniques such as HPLC and GPC (for separation of monomers, dimers and trimmers etc.) for separation of peptides.

• Characterization of the peptides was performed using analytical instruments such as Mass spectrometer, UV-Vis spectrometer, NMR, FT-IR and Fluorimeter.

• Acquired data using the MassLynx software and quantified with the Driftscope software.

• Molecular modeling was performed using Gaussian and Spartan computer programs.

• Achieved excellent results.

• The peptide designed and synthesized with sequence acetyl-Cys1-His2-Cys3-Gly4-Pro5-Cys6-His7- Cys8 with higher number of active binding sites, the cysteines, led to a considerable increase in the Cu(I) and Zn(II) binding, oligomerization and redox activity. Analytical trainee

Aurobindo Pharma - Hyderabad, Telangana

July 2012 to October 2012

• Took part in the process of drug formulation, method development and method validation.

• Extensively used mass spectrometer, GC, GC/MS, HPLC for Qualitative analysis.

• Assisted in drug product analytical method development and quality systems i.e. Laboratory Investigation Reports (LIR), Corrective and Preventive Actions (CAPA), calibration, validation, preformulation and change control employing standard operating procedures SOP.

• Worked under cGMP, GLP and OSHA guidelines and FDA regulations in manufacturing of various pharmaceutical products.

• Gained knowledge and experience in QA and QC, R&D stability testing and ANDA. Education

Master of Science in Research

Texas A&M University - Commerce, TX

2016

Texas A&M University "Jaine and SuzzaneNagarkatti

September 2014 to March 2015

MM

Texas A&M University

March 2014 to June 2014

Bachelor of Pharmacy in chemistry

Osmania University - Hyderabad, Telangana

Skills

• Chromatography

• Gcms

• Spectrometer

• Quality control

• Instrumentation

• Sas

• Titrations

• Ir

• Beckman

• Lims

• Clinical sas

• Chemistry

• Ion

• Wet chemistry

• Nmr

• Uv-vis

• Purification

• Titration

• Icp

• Exchange

Additional Information

Skills

Analytical Skills:

• Knowledge in spectrometric and chromatographic techniques (normal and reverse phase chromatography), Quadrupole MS/MS studies of peptides, protein precipitation, particle size analysis, separation and purification techniques, analytical data interpretation.

• UPLC, LC/MS/MS and GCMS Method development of detecting and quantification of various analytes.

• Knowledge and practice in column chemistry, ion exchange chromatography, distillation, liquid- liquid and solid phase extraction techniques, wet chemistry, Potentiometric & Karl Fisher titration and residue determination.

Technical skills

• Mass Lynx, Empower, Lab solutions, Multiquant, Driftscope and Spartan softwares.

• LIMS, Electronic notebooks (ELN).

• MS office (Excel, word and power point)

• Minitab, SPC, Six sigma, Acid-base titrations softwares.

• Clinical SAS

Instrumentation skills:

• Mass spectrometer, UPLC, LC/MS/MS, GCMS, GC-Headspace, UV-Vis spectrometer, NMR, FT-IR, ICP- MS.

• pH meter, Karl Fischer titrators, Malvern & Beckman Coulter particle size analyzers Leadership skills:

• Led a research team on multiple projects, trained new analysts on analytical instruments and techniques.

• Trained my co-researchers on proteomics and promoted the applications of proteomic studies.

• Led the team as the Quality control manager at MM Lab, Inc. successfully.

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