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Lab Technician Data

Location:
Tucson, Arizona, United States
Salary:
45,000
Posted:
April 21, 2017
Email:
aczw87@r.postjobfree.com

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STACY JOHNSON

** *. ***** **** **. Apt.****

Tucson Arizona 85704

(928) ***-****

aczw87@r.postjobfree.com

QUALIFICATIONS SUMMARY

Acute attention to detail, focused, tenaciously strive to achieve goals, former masonry foreman

Years of experience in the workforce, dependable, determined, work well with a team or alone

Keen inductive and deductive reasoning, sharp memory, data processing and computer skills

Former business owner, skilled at advertizing and customer satisfaction

EDUCATION

Northern Arizona University, Flagstaff Arizona

Master of Science, Biology, GPA 3.70, August 2010

Northern Arizona University, Flagstaff Arizona

Bachelor of Science, Cellular and Molecular Biology, Chemistry minor, GPA 3.54, May 2008

Yavapai Community College, Prescott Arizona

Associate of Science, GPA 3.46, August 2005

American Career Builders Truck Driving School, Tucson, Arizona August 2013-October 2013

Student/Graduate. Certificate CDL-A Commercial Truck Driving

HAZMAT

Tanker

Doubles/Triples

EMPLOYMENT

Northwest Colorado Transport

Vac-Truck operator, Killdeer, ND August 2014-Present

Bakken Energy, Miller’s Midnight Trucking March 2014- August 2014

Vac-Truck operator, Williston, Watford City, ND

Reynolds Logistics, Tucson Arizona September 2013-March 2014

Team Over The Road driving

Averaging 2600 miles per week

Doing masonry side jobs on my own, March 2013-August 2013

searching for employment, Tucson Arizona.

Nesco Resources,Ventana Medical Systems, Tucson Arizona October 2012-March 2013

Research Associate R@D

Performed pH measurements on numerous solutions

Prepared reagents in various molar concentrations and dilution percentages

Used Karl Fischer titration Coulometer to determine H20 content in solutions

Collected and then entered large amounts of data via excel spreadsheets

Measured and weighed various mechanical parts for material compatibility

Operated experimental haematoxylin and Eosin staining machines

Ran biopsy specimen slides through H@E machines

Prepared and signed various forms and paper work

Kept meticulous lab book entries

Researched/read peer reviewed scientific journals

Understands and follows good lab practices

Caris Life Sciences, Phoenix, Arizona September 2011-October 2012

Laboratory Aide/Molecular Lab Technician

Performed RNA extraction from patient cancer biopsies via BioRad instruments

Utilized sterile pipepetting skills in multiple daily samples

Quantified/standardized RNA samples via nanodrop BioRad

Synthesized patient DNA via assay using company SOPs

Performed PCR/RT-qPCR

Extracted RNA/DNA from patient blood samples and frozen tissue samples

Analyzed patient RT-qPCR data daily

Used various company computer software programs

Doing masonry side jobs on my own, April 2011-October 2011

searching for employment, Phoenix, Arizona.

A-Tek inc/Homeland Security, State Dept. of Health, Phoenix, Arizona October 2010-April 2011

Microbiologist, Bioterrorism BCL-3 Lab

Performed RNA extraction from filters placed around the city

Maintained Chain of Custody paper work for samples

Utilized sterile pipepetting skills in multiple daily samples

Synthesized DNA from RNA extracted from filter samples

Performed PCR/RT-qPCR

Analyzed samples PCR/RT-qPCR / for presence and concentrations of 5 BCL-3 bacterial pathogens

Used various company computer software programs

Doing masonry side jobs on my own, August 2010-October 2012

searching for employment, Tucson Arizona.

Completing data analysis and thesis writing, Flagstaff Arizona. June 2010-August 2010

Northern Arizona University, Flagstaff Arizona. June 2008- June 2010

Lab technician/Graduate

Maintained cell cultures with Dulbecco’s glucose media, infected cell cultures with protozoan parasites

Extracted mRNA and proteins from cultured cells using appropriate lysis buffers, prepared all buffers

Prepared and loaded lanes after cDNA synthesis and PCR, ran gel electrophoresis via Biorad minicycler

Performed SDS-PAGE and western blot, applied primary & secondary antibodies to PVDF membranes

Prepared ECL reagents for immunoblots, transferred to X-ray film,

Stripped membranes applied anti-actin antibodies, performed RT-PCR via Biorad, standards+melt curve

Used siRNA knockdown transfection kits, entered data in Excel, interpreted data, thesis and manuscript

Taught Bio 100 lab classes

Background in cell biology, microbiology, virological, mycological, and parasitological lab techniques

Performed biochemical and microscopic analyses on purified bacterial and viral cultures

Completing undergraduate college course work, Prescott, Arizona. June 2002-June 2008

Doing masonry side jobs on my own, Prescott, Arizona. November 2001-June 2002

Two home businesses, Keapana Horsemanship trail rides, and September 1996-November 2001

Keapana Services activity referral, Kauai, Hawaii. Co-business owner

Completed business proposal, managed creative advertizing, scheduled trail rides

Fed and watered horses, repaired fences

Referred trairide clients and other visitors to other island activities via website with discount card

Worked on formulating and building Keapana Horsemanship trail rides, and Keapana Services

activity referral businesses March 1996-September 1996

ACE Certified Personal Trainer, Personal December 1993-March 1996

Fitness Trainer, Honolulu, Hawaii.

Instructed clients on fitness machinery, tailored exercise/diet regimes for clients of all ages and genders

Measured heart rates and calculated VO2 max for aerobics clients, underwent CPR certifications

Measured body composition, took blood pressure, rendered encouragement and guidance

ACE Certified Personal Trainer training course studies. November 1992-December 1993

Honolulu, Hawaii

Masonry Construction foreman/supervisor, journeyman brick/block layer May 1977- November 1992

Spread mortar on brick/block using trowel, measured squared and plumbed various masonry structures

Read blueprints for structural steel reinforced commercial buildings, supervised worksite construction

Performed initial layout of numerous commercial and residential buildings, built decorative brick patios

Worked with city inspectors and engineers to ensure structural integrity of block walls

HONORS, AWARDS

Graduated cum laude, Northern Arizona University, August 2010

Awarded government SMART grant 4 times for B+ average in advanced science classes 2007-08

Invited to the Golden Key International Honor Society 2007-10

Dean’s list for 2 semesters Yavapai College

References

Dr. Maribeth Watwood. Professor and chair of the biology department at Northern Arizona University (NAU). aczw87@r.postjobfree.com. Phone, (928)-***-****.

Dr. Catherine Propper. Professor of Endocrinology NAU. aczw87@r.postjobfree.com. Phone, (928)-***-****.

Dr. Bruce Hungate. Professor and chair of environmental science at NAU. aczw87@r.postjobfree.com

Phone, (928)-***-****.

Dr. Nancy Johnson. Professor of environmental science at NAU. aczw87@r.postjobfree.com. Phone, (928)-***-****.

Dr. Fernando Monroy. Professor of Immunology at NAU. aczw87@r.postjobfree.com. Phone, (928)-***-****.

Dr. Russel Bull. Vocational Rehab. Phone, (928)-***-****. aczw87@r.postjobfree.com.

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