JAGMOHAN SINGH

Work: 215-***-****

Cell: 831-***-****

Email: aczgdx@r.postjobfree.com

Current Status in US: US Citizen

CURRENT POSITION:

Post-doctoral fellow,

Dept. of Medicine,

Thomas Jefferson University (TJU), Philadelphia.

Area of Research: G-protein coupled receptor pharmacology in lower Gastrointestinal Smooth Muscle.

EDUCATION:

Doctor of Philosophy in Biotechnology and Cancer Genomics, 2007

Punjab University, Chandigarh, India.

Thesis title: Genomic susceptibility in esophageal cancer patients.

Major Emphasis: Cytochrome P450 and HPV as biomarkers associated with esophageal cancer patients.

Professional Courses Completed: 2013

Certificate course in laboratory techniques in Molecular Biology, TJU Philadelphia, USA.

RESEARCH EXPERIENCE & SKILLS:

8+ Years of research experience in Pharmacology, Molecular biology and Physiology.

Developing and validating drug discovery protocols by using in vitro and in vivo models

Expertise in Cell Culture, DNA, RNA. ELISA, Transfection, Transduction, Tissue culture, siRNA, miRNA and their expression analysis, Confocal Microscopy, Immunofluorescence (Immunocytochemistry and Immunohistochemistry).

Experience of Force Transducer experiments related to smooth muscle physiology and drug testing protocols, analyzing and plotting data from drug analysis experiments

Experience in data analysis by using Prism 5, Ingenuity Pathway Analysis and other basic software such as Word, PowerPoint and Excel.

Knowledge of all major databases and bioinformatics sources such as Pubmed, Ensemble, miRbase, Miranda, Targetscan etc.

Experience in working with different animal models, Rat and Mice

GRANTS WRITING EXPERIENCE:

Support Personnel

NIH Grant: RO1: Neurohumoral Control of IAS.

Designed project, written methodology and acquired preliminary data.

Worked on major aims of the grant.

Aim: 1. identify intracellular signaling molecules that regulate IAS tone.

Aim: 2. identify miRNAs that regulate these intracellular signaling molecules, and develop strategies to reverse the miRNA-regulated decrease in IAS tone with age.

Planned experiments, organized data and written manuscripts.

Co-Investigator

Industrial Grant: CSL Behring Investigator Initiated Study Application (Immunology R&D): Characterization of Scleroderma Immunoglobulins (SScIgGs) Against Muscarinic-type3 (M3-R)-Receptor.

Designed Project, written methodology and acquired preliminary data.

Designed experiments to check the effect of Intravenous Immunoglobulin (IVIG) on Anti M3R autoantibodies in SSc patients.

Performed studies and analyzed data.

Helped in organization of results and manuscript.

Assistance in designing projects for MD, PhD students

Immunocytochemistry analysis of scleroderma patient antibodies and its correlation with Disease type in patients involving gastrointestinal manifestation (Sumit Kumar, Singh J, et al., AJP, 2016 accepted).

Heme Oxygenase-1 Up-regulation Modulates Tone and Fibroelastic Properties of Internal Anal Sphincter (Krishna CV, Singh J, et al., Am J Physiol Gastrointest Liver Physiol. 15; 307(6):G595-601, 2014).

SM fascicular reorientation is required for esophageal morphogenesis and dependent on Cdo. (Romer A, Singh J, et al., J Cell Biol. 201(2):309-323, 2013.)

MAJOR JOB RESPONSIBILITIES

As a Post-doctoral fellow at TJU, Philadelphia Mar. 2008 - Present

Designing and performing experiments and analyzing data, making power point presentations and writing manuscripts.

Designing protocols, maintaining cell lines and primary cell culture, performing qPCR, western blotting, immunofluorescence, ELISA or any new innovative experiments based on the requirement of experimental ideas.

Keeping research record, updating chemical inventories, keeping animal record, maintaining lab instruments, ordering chemicals, working with IBC, IACUC for approval of lab safety and animal protocols.

As a research Fellow at Temple University Nov. 2006 - Feb. 2008

Adenovirus preparation in HEK293, Genotyping, studying Glutamate receptors in signaling pathways involved in senescence, Growing and maintaining different breast cancer cell lines.

PUBLICATIONS

1.Singh J, Mohanty I, Addya S, Phillips B, Yong MH, An SS. Penn RB, Rattan S., Role of differentially expressed microRNA-139-5p in the regulation of phenotypic internal anal sphincter smooth muscle tone (submitted: Scientific reports).

2.Singh J, Boopathi E, Addya S, Phillips B, Rigoutsos I, Penn RB, Rattan S. Aging-associated changes in microRNA expression profile of internal anal sphincter smooth muscle: role of microRNA-133a. Am J Physiol Gastrointest Liver Physiol. 311(5):G964-G973, 2016.

3.Singh J, Kumar S, Rattan S. Bimodal effect of Oxidative Stress in IAS SM.Am J Physiol Gastrointest Liver Physiol. 308(11):G924-933, 2015

4.Singh J, Kumar S, Krishna CV, Rattan S. Aging-associated oxidative stress leads to decrease in IAS tone via RhoA/ROCK downregulation. Am J Physiol Gastrointest Liver Physiol. 306:G983-991, 2014.

5.Singh J, Rattan S. Role of PKC and RhoA/ROCK pathways in the spontaneous phasic activity in the rectal SM.Am J Physiol Gastrointest Liver Physiol.; 304(8):G723-731, 2012.

6.Singh J, Cohen S, Mehendiratta V, Mendoza F, Jimenez SA, Dimarino AJ, Rattan S. Effects of Scleroderma Antibodies and Pooled Human Immunoglobulin on Anal Sphincter and Colonic SM Function. Gastroenterology. 143(5):1308-1318, 2012.

7.Singh J. and Rattan S. Development of Myogenic IAS Reconstructs from Human IAS smooth muscle cells (SMCs) with Functional and Molecular Properties Similar to Intact Human IAS Am J Physiol Gastrointest Liver Physiol. 303(6): G713-722, 2012.

8.Singh J, Maxwell PJ 4th, Rattan S. Immunocytochemical evidence for PDBu-induced activation of RhoA/ROCK in human IAS SMCs. Am J Physiol Gastrointest Liver Physiol. 301(2): G317-G325, 2011.

9.Singh J, Mehendiratta V, Del Galdo F, Jimenez SA, Cohen S, DiMarino AJ, Rattan S. Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in IAS SMCs. Am J Physiol Gastrointest Liver Physiol. 297(6): G1206-G1213, 2009.

ABSTRACTS AND MEETING PRESENTATIONS:

1.Singh J, Kumar S, Phillips, Rattan CS. Nature of Extracellular Signal for the Basal IAS SM Tone in Humans. Gastroenterology, Vol. 148, Issue 4, S-303–S-304, 2015.

2.Singh J, Addya S, Fortina P, Rattan CS. MicroRNA-139-5p (miR-139-5p) in the Myogenic Basal Tone of IAS vs. the Phasic Rectal SM (RSM): Studies in Purified SMCs Gastroenterology, Vol. 144, Issue 5, S-364, 2013.

3.Singh J, Cohen S, Mehendiratta V, Jimenez SA, DiMarino AJ, Rattan S. Effect of pooled Human Intravenous globulin (IVIG) on the Reversal of Cholinergic Inhibition of SM by IgG Immunoglobulins from Patients with Scleroderma (Scl) Gastroenterology, Vol. 143, Issue 5, p1308–1318, 2013. (Poster of distinction Awarded).

4.Singh J. and Rattan S. Development of Myogenic IAS Reconstructs from Human IAS SMCs with Functional and Molecular Properties Similar to Intact Human IAS Gastroenterology, Vol. 142, Issue 5, S-898, 2012.

5.Singh J, Mehendiratta V, Del Galdo F, Jimenez SA, Cohen S, DiMarino AJ, Rattan S. Immunoglobulins from scleroderma patients inhibits the muscarinic receptor activation in IAS SMCs. Gastroenterology, Vol. 136, Issue 5, A-220, 2009.

PRESENTATIONS

Therapeutic potential of Intravenous Immunoglobulins (IVIG) in Systemic sclerosis patients. TJU, Philadelphia.

Isolation of Human Internal Anal Sphincter Smooth Muscle cells and Preparation of IAS reconstructs. TJU, Philadelphia.

PROFESSIONAL AWARDS AND HONORS

Travel fellowship award was awarded to Visit Capitol Hill, Washington, DC as a member of Coalition of Life Sciences (CLS); Capitol Hill Day in Washington, DC in support of increase in NIH funding for scientific research April 2, 2010 and 2013.

Received travel fellowship award from TJU to Attend DDW meeting held at Chicago in 2009.

Received Research Fellow award from Temple University Philadelphia in 2006.

Qualified University Grants Commission examination conducted by Indian Govt. for research Fellowship, 2002.

MEMBERSHIPS:

Vice President of Social Affairs Post-Doctoral Association TJU, 2014.

Member of American Association for the Advancement of Science (AAAS), 2013.

International Committee of Postdoctoral Fellows at TJU since Jan. 2009.

American Gastroenterology Association, 2009.

REFERENCES:

Available Upon Request

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