Microsoft Office Technician

Amanda Lynn Cummings

*** *** ****** *****, ****** GA 30605 *.****.********@*****.*** 619-***-**** EDUCATION

San Diego State University San Diego, CA

Bachelor of Science Biology May 2014

PROFESSIONAL CERTIFICATIONS

American Association for Laboratory Animal Science Assistant Laboratory Animal Technician (ALAT) July 2012 INSTRUMENTATION, LABORATORY AND SOFTWARE EXPERIENCE Instrumentation: FPLC (GE AKTA PURE), UV/VIS (Thermo Scientific Nanodrop 2000C), Imaging (GE ImageQuant LAS 4000), BioMek, Tecan, Hamilton Liquid Handler

Laboratory: Affinity Chromatography, Size Exclusion Chromatography, Western Blots, SDS-PAGE, RT-PCR, Directed Evolution, ELISA assays, Various Assay Development (PicoGreen, Catalase, Acid Phophatase, Coomassie Blue G-250, Nextera XT, Qubit) Software: Microsoft Office Suite, Unicorn 7.0, PatentSafe (ELN), LIMS, Quartzy, Logger Pro RELEVANT EXPERIENCE

Glycosensors and Diagnostics Research Associate Athens, GA August 2016 – February 2017

Under the supervision of lead scientists, identified 25 Lectens® candidates via a yeast library display system for 3 product lines

Performed and optimized directed evolution techniques to express identified candidates in an E.Coli expression system

Developed and optimized manual affinity purification methods increasing workload by 3x

Performed automated affinity purification (IMAC) and Size Exclusion Chromatography to generate suitable quantities of purified protein

Developed and optimized a protocol which will eliminate Size Exclusion chromatography resulting in a 97% reduction in cost of production.

Optimized quality control protocols for reagent development using SDS-PAGE and Western blot techniques

Presented scientific findings weekly to multidisciplinary team Alere San Diego Lead Sample Bank Research Associate San Diego, CA February 2015 – July 2016

Direct liaison and trainer of two technicians on sample receiving, processing and storage of clinical trial samples

Package, ship and track Clinical samples international and nationwide

Clinical Trial Diabetes Study coordinator

Audit central clinical files to ensure accuracy and completion

Organized and cataloged 1 million clinical trial samples in over 25 -80 freezers, to ensure database accuracy

Order and stock supplies for Sample Bank and Clinical personnel Belcan-Monsanto Contract Biologist Woodland, CA May 2014 – December 2014

Under ISO9000 environment, perform Seed washes, DNA isolations and Real time PCR on vegetable seeds

Coordinated with Sample management, schedule and perform routine ELISA assays for bacterial pathogens

Track and log sample assay results for greenhouse grow-out scheduling and customer results

Developed and implemented automated SOPs using Hamilton and Biomek machinery which increased workload capacity 250%

Suna- Alere San Diego Contract Research Technician San Diego, CA June 2011 – September 2013

Under supervision of lead scientist, create media, buffers, antibiotic plates and solutions for 3 laboratories

DNA and RNA isolations and purifications of E.coli expression systems

Under supervision of Animal Technician, animal cage washing and preparation

Orbital test bleeds and spleen extractions of mouse colonies

Database entry of bleeds and extracted tissues

Working with vendors, saved the company in $10,000 in chemical products REFERENCES

Upon Request

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