Post Graduated in Biotechnology

SONALI KAPOOR

**** ******** ***

Mundelein

Illinois, 60060

Phone no: 224-***-****

**************@*****.***

PROFESSIONAL ABRIDGEMENT

Dedicated, service-oriented team member; motivated to work efficiently without direct supervision in busy environment, handling many tasks simultaneously. Adhere to company policy and rules.

Exceptional organizational skills; able to prioritize workload and multi-task to meet deadlines.

Well-developed communication skills demonstrated through interaction with students and colleagues and the ability to establish and maintain professional relationships.

Good listener. Interact positively with a wide range of people of various ages, cultures and socio-economic levels. Display empathy and consideration for others.

Master in Science in Medical Biotechnology.

Bachelor in Science (Major in Zoology) from University of Delhi.

Worked as High School teacher for few months and was also providing volunteering tutoring to underprivileged students.

6 months project research experience in Molecular biology.

Experience in MS Office (Excel, word, Power point).

PREVIOUS EXPERIENCE

July’09-Nov’09 Little Angels High School, India Science Teacher

Attainments:

Handled 4 classes 7th to 10th, teaching Science (Physics, chemistry & Biology).

Biology Laboratory in charge.

Co- Sponsored an after-school Science Club to provide students with extra support.

Integrated technologies for visual representation like MS PowerPoint, Educational websites, streaming videos.

AREAS OF EXPERTISE

Molecular Biology: SDS-PAGE and Agarose Gel Electrophoresis, Isolation of Genomic and Plasmid DNA, Restriction Digestion and Ligation of DNA molecules, Competent Cell Preparation, Transformation by Calcium Chloride Heat Shock Method, PCR, Growth curve studies in E.coli, Bacterial gene expression - Induction of Lac Operon in E.coli, Selection of transformed cells by Blue White Screening.

Genetics: DNA isolation- Both bacterial and Mammalian, amplification of gene- PCR, Genotyping RFLP method, Isolation, purification and quantization of genomic DNA from mammalian blood using phenol chloroform extraction method, PCR based techniques like RAPD, SNP, AP-PCR, ERIC-PCR.

Immunology: Blood group estimation; Ouchterlony Double Immunodiffusion, Radial Diffusion, ELISA, Western blotting, Performed tests on immunoserums and antibodies produced from rabbits.

Biochemistry: Protein and Carbohydrate Estimation, Chromatography – Paper & Thin Layer, Dnase & Rnase digestion of DNA.

Biophysical: UV-Visible Absorption Spectroscopy.

Bioinformatics: Outlook, Excel, Powerpoint, Word, VB Scripting, NCBI data model, Study of gene bank sequence, Study of phylogeny, DNA Sequence submission, Primer designing, Human genome Sequence Study, Database of NCBI, Genome analysis, DNA Polymorphism study, SNP, etc.

Cell Biology: Histology of tissues, Basic histological techniques like tissue fixation, embedding, Microtomy, staining, Light Microscopy.

RESEARCH EXPERIENCE

Completed Masters Dissertation research under guidance of Prof. B.D Lakchaura, Prof. G.B. Pant University, Pant Nagar, India. My work was on Salmonella, pathogenic bacteria and involved isolation of this bacterium from the water samples collected from the Naini Lake of Nainital. This lake is a major water source for both humans and animals for the city itself and other suburban areas. The objective of the study was to assess the current status of Salmonella contamination in the Naini Lake and standardization of AP-PCR (Arbitrarily primed polymerase chain reaction) for molecular typing of isolates obtained from the water samples. The isolation technique involves both culture and DNA based detection techniques. The water sample was filtered through 0.4-micron filter paper, and then enriched in tetrathionate broth. The samples were screened for the presence of Salmonella by hisA gene targeted PCR. The samples showed positive result in hisA gene PCR and were streaked in Brilliant Green Agar. AP-PCR was used for molecular typing of the isolates obtained from the water samples. AP-PCR is also referred to as the randomly amplified polymorphic DNA assay employing a single short (typically 10 base pairs) primer that is not targeted to amplify any specific bacterial DNA sequence. The research work is examined by Doctors and professors from AIIMS, PGI in the final year viva.

EXTRA-CURRICULAR ACTIVITIES

Active member of Student cultural group in UFHT Medical College.

Ranked First in Debate Competitions in English and Hindi in the School at A.E.S School.

Got First Prize in Science Fair organized in A.E.S School.

Got first Prize in All India Art Competition.

Member of Winning Team in Science and General Knowledge Quiz competitions a number of times at the A.E.S School.

VOLUNTARY COMMUNITY WORK EXPERIENCE

Engaged as Student volunteer in Dr.Shusheela Tiwari Memorial Forest Hospital, Uttarakhand, India.

Worked in rehabilitation centers for short term under National Institute of Forensic Science & Criminology, Ministry of Home Affair, Delhi.

Coached underprivileged children in Mathematics and Science in Uttarakhand.

Worked as Teacher’s Assistant in Biochemistry and Biotechnology lab in Dept. of Biochemistry/ Biotechnology, UFHT Medical college to set up experiments.

Worked in Biochemistry lab. at Dr. Shusheela Tiwari Memorial Forest Hospital.

COMPUTER EXPERTISE

Knowledge of Windows, MS Office, Internet Explorer.

Basic knowledge of usage of various databases like NCBI, Entrez, pubmed.

Languages : English, Hindi.

DATE OF BIRTH: 01st March, 1984

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