Yajuan Li, Ph.D.

Phone: 781-***-****

Email: acyvuy@r.postjobfree.com

Visa status: Green card

Summary

. Self-motivated scientist with high enthusiasm in assay development and

drug discovery to improve the new product application in disease

diagnosis and treatment

. Experienced expert in molecular biology, cell biology, oncology,

immunology, in vivo and in vitro assay, BSL2, CLIA lab

. Goal-oriented team player with skills in conducting collaborative

projects across multidisciplinary teams

PROFESSIONAL EXPERIENCE

Kew Inc., Cambridge, MA

Scientist

3/2016-current

. Maintain and manage Biomek FX and NX liquid handling automation

platforms

. Develop and optimize new methods by using liquid handling automation

to support next generation sequencing (NGS)

. Perform NGS (DNA extraction, library preparation and capture) in

manual as well as high throughput automation, run sequencing through

illumina Nextseq and Miseq

. Create and optimize standard operating protocols for laboratory-based

methods both manual protocol and automated protocol

. Design digital PCR to validate paneled sequencing data

. Run QC, validate and make report for new assays and new methods

. Conduct basic data analysis

Verastem, Needham, MA

Scientist

04/2015-10/2015

. Utilized flow cytometry to detect CSC biomarker in different cancer

cells

. Isolated CD 8+ T cell and T reg cell from human PBMC and established

cell culture condition, further evaluated small molecule drug

regulation role in these immune cells

. Validated small molecule drug and immunotherapeutic agents combination

effect on immune cell function during cancer immunotherapy

. Performed cell-based function assay, proliferation assay, cytokine

ELISA and T cell receptor flow cytometry assay

. Validated the small molecule and other chemotherapeutic drugs

combination efficacy for cancer chemotherapy

Microarray core facility, Department of Immunology, UTSW medical center,

Dallas, TX

Postdoc researcher

03/2010-01/2015

. Isolated and cultured mesenchymal stem cell, induced and identified

the differentiation potential of mesenchymal stem cell

. Focused on investigating the treatment effect of the target gene

modified mesenchymal stem cell implantation on immune-mediated

nephritis

. Screened disease biomarker by immunoassay to determine the specific

biomarkers associated with disease development and diagnosis

. Performed qPCR to identify gene expression level and utilized ELISA

and luminex multiple immunoassay to detect cytokine/chemokine protein

in serum sample

. Conducted in vitro cell based assays to evaluate gene function,

identified cell vitality, apoptosis, toxicity, immunohistochemistry to

identify lymphocyte infiltration in tissue

. Isolated DNA/RNA from FFPE tissue, fresh tissue and cell lines,

DNA/RNA quality bioanalyzer assay, conducted Taqman qPCR, illumina

gene expression assay, protein microarray

. Supervised graduate students and interns. Wrote and prepared proposal

for grant application. Prepared peer-reviewed manuscripts

Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China

Researcher

06/2009-02/2010

. Isolated and cultured mouse embryonic fibroblasts as feed layers to

support human stem cell culture and maintenance

. Explored the erythroid differentiation of hematopoietic stem cells

. Utilized RNAi technique and lentivirus vector to knockdown target gene

to explore differentiation mechanism of hematopoietic stem cells

. Supervised graduate students, and interns in academic setting. Led

research projects, planned experiments, reviewed and coordinated

experimental protocols, reviewed data, wrote and oversaw preparation

of manuscripts, provided expertise for projects, and laboratory

management

Peking University Health Science Center, Department of Microbiology,

Beijing, China

PhD candidate

09/2003-06/2009

. Investigated molecular mechanism of functional T-type calcium channel

in modulating hepatocellular carcinoma cell proliferation

. Explored the association between EGF SNPs and the genetic

susceptibility of hepatocellular carcinoma development

. Tested protein levels in serum samples by ELISA to identify biomarkers

related with disease development and diagnosis

. Performed cell apoptosis assay and observed cell morphology

. Utilized siRNA knockdown target gene in cell line and confirmed gene

knockdown efficiency

. Isolated virus DNA and RNA, identified virus genotype, analyzed

hepatitis virus genotype distribution and performed epidemiologic

analysis

SKILLS

Cell Biology: Stem cell, primary cell, cancer cell culture, 3D culture, DNA

and RNA delivery, flow cytometry, cell transfection, cell differentiation,

cytotoxicity, proliferation, apoptosis assay, lentivirus packaging system,

cell transduction, cell immunocytofluorescent assay, fluorescence

microscope

Molecular Biology: DNA cloning, PCR, RT-PCR, qPCR, plasmid preparation and

purification, bacterial transformation, siRNA, CRISPR/Cas9, protein

purification, molecular hybridization, SDS-PAGE, western blot, ELISA,

luminex immunoassay, gel electrophoresis, DNA point mutation, deletion, SNP

detection

NGS and microarray: Illumina and Affymetrix gene expression assay, NGS

fragment library preparation and capture, sequencing, DNA/RNA isolation,

DNA/RNA quantity and quality assay, Taqman qPCR assay, QC assay

Data analysis: NGS data analysis tools with R and linux system

Histopathology: Frozen section and paraffin section preparation, tissue

slide immunohistochemistry assay

Animal Experiment: Immune-mediated nephritis mouse model, ip, iv, sc route

injection, mouse embryonic fibroblasts (MEFs) isolation, draw blood

(orbital, tail, submandibular), collect urine and tissue

Computer Software: Office (Microsoft Word, Excel, and PowerPoint), SPSS,

Vector NTI, BioEdit, BeadStudio, Genespring, GenePix4000B, IPA, GraphPad

Prism, Image J, FlowJo, R, Ubuntu, IGV

EDUCATION

Peking University, Health Science Center, Beijing, China Ph.D.,

Microbiology, molecular biology

Peking University, Health Science Center, Beijing, China M.S.,

Microbiology, molecular biology

Recent Publications (22 peer-reviewed publication in total)

1. Yajuan Li, Mei Yan, Jichen Yang, Indu Raman, Yong Du, Soyoun Min,

Xiangdong Fang, Mohan Chandra, Quan-Zhen Li. Glutathione S-transferase Mu

2 transduced mesenchymal stem cells ameliorated anti-GBM antibody-induced

glomerulonephritis by inhibiting oxidation and inflammation. Stem Cell

Research & Therapy. 2014, 5: 19.

2. Yajuan Li, Wei Li, Chu Liu, Mei Yan, Indu Raman, Yong Du, Xiangdong

Fang, Xin J. Zhou, Chandra Mohan, Quan-Zhen Li. Delivering oxidation

resistance-1 (OXR1) to mouse kidney by genetic modified mesenchymal stem

cells exhibited enhanced protection against nephrotoxic serum induced

renal injury and lupus nephritis. Journal of stem cell research and

therapy, 2014, 4:231.

3. Mukesh Bansal, Jichen Yang, Charles Karan, Michael Patrick Menden, James

C. Costello, Hao Tang, Guganghua Xiao, Yajuan Li, Jeffrey Allen, Rui

Zhong, et al. The challenge of predicting synergistic and antagonistic

compound-pair activity from individual compound perturbations. Nature

Biotechnology, 2014, 32:1213-22.

4. Jichen Yang, Hao Tang, Yajuan Li, Rui Zhong, Tao Wang, Stephen T.C.

Wong, Guanghua Xiao, Yang Xie. DIGRE: Drug Induced Genomic Residual

Effect Model for Successful Prediction of Multidrug Effects.

Pharmacometrics & Systems Pharmacology, 2015, 4:e1.

5. Yajuan Li, Indu Raman, Yong Du, Mei Yan, Soy Min, Jichen Yang, Xiangdong

Fang, Wei Li, Jianxin Lu, Xinjin Zhou, Chandra Mohan, Quanzhen Li.

Kallikrein transduced mesenchymal stem cells ameliorate anti-GBM disease

and lupus nephritisby ameliorating inflammation and oxidative stress.

Plos One. 2013; 8:e67790.

6. Yajuan Li, Xiangdong Fang, Quanzhen Li. Biomarker prolifing for lupus

nephritis. Genomics Proteomics Bioinformatics. 2013; 11:158-165.

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