PURBASA PATNAIK

**** ******** **, *** *****, CA- 94024

Phone: 415-***-****

Email: acysdu@r.postjobfree.com

TECHNICAL SKILLS

? Cell Culture: Mammalian Cell Lines & Bacterial Culture Maintenance, Transfection & Transformation

? Cell Based Assays: TCID50 Assay, BCA Assay, Sucrose Assay, Toxicity Assay, Interference Assay, Chromogenic Assay, Protein Estimation.

? Molecular Biology: Cloning, DNA/RNA purification, DNA quantification, RT-PCR, q-PCR, Gel Electrophoresis, plasmid purification, Western Blotting

? Formulation & Analytical: DoE, Stability studies, CE-SDS, cIEF, CD Spectroscopy, ELISA, LyoSMART lyophilizer, Karl Fisher (KF), HPLC, Differential Scanning Fluorimetry, Dynamic Light Scattering, HIAC, Differential Scanning Calorimetry, Micro-flow Imaging, Viscosizer, UV/Vis, Tecan Liquid Handling Systems, Vial and Syringe Hand Fill, Protein Concentration, pH, Viscometer, Osmometer, Density Meter, Conductivity Meter, DO meter, Lighthouse Headspace Analyzer, Solo VPE, SEC-HPLC, freeze-dry microscopy.

? Other: MS Office, JMP Analysis, GMP qualified

WORK EXPERIENCE

Cook Pharmica, USA (Sept?14-Present)

Associate Scientist, Formulation Development

? Led, planned, and executed formulation development & process development activities in support of biologics and small molecule.

? Conducted DOE, formulation screening, stability studies, protein characterization, forced degradation and managed stability programs.

? Developed lyophilization cycles and characterized lyophilized drug product using analytical methods.

? Characterized drug product processes by executing process compatibility, mixing stress, filter flush, mixing speed, IV bag and set compatibility, extractable & leachable studies.

? Assisted GMP manufacturing during process failures by studying potential causes and troubleshooting technical issues.

? Interacted and collaborated with clients on a regular basis to report analyzed data and take scientific decisions.

? Worked in cross-functional teams (Analytical Development, QC, Tech Services, Project Management, Manufacturing) for successful client campaigns within set budget and timeline.

? Authored and reviewed broad range of batch records, proposals, protocols, technical/test reports and SOPs.

Bayer Healthcare, USA (Sept?13-Sep?14)

Associate Scientist, Pathogen Safety

? Facilitated experimental studies to ensure Bayer biologics meet international pathogen safety standards through cross functional collaboration.

? Planned, developed and executed experiments independently to evaluate effect of various chemical agents on viral inactivation to help GMP manufacturing.

? Conducted data analysis and report review by collaborating with the quality assurance team prior to regulatory submissions.

Eli Lilly Company, USA (Sept?12- Mar?13)

Co-op Intern, Bioprocess Virology & Purification Development

? Broadened the utility of depth filters in biologics production by evaluating their viral clearance capacity.

? Designed filtration experiments and TCID assays to study viral clearance by different types of depth filters.

? Studied & correlated effect of size, pH and salt conditions in working of depth filters helping Eli Lilly to expand its use in viral clearance.

Robert H. Lurie Comprehensive Cancer Center, USA (Jan?12-May?13)

Graduate Research Scholar

? Designed micro RNA (miRNA) based dual biosensor plasmids to identify & isolate Cancer Stem Cells (CSCs).

? Utilized molecular biology techniques to construct fluorescent plasmid sensors.

? Evaluated the functionality of the biosensors by studying miRNA expression in various cell lines using qRT PCR, FACS, UV-Vis spectroscopy, transfection and transformation.

? Increased the isolation to 7 folds making the sensor system a better alternative to isolate CSCs as compared to conventional methods.

EDUCATION

? Northwestern University, Evanston (IL), USA (2013)

Master of Science, Biotechnology

? Jaypee Institute of Information Technology, Noida, INDIA (2011)

Bachelor of Technology, Biotechnology

Additional Training

? IvyTech Corporate College (IN), USA- Six Sigma Green Belt Training (2015)

PUBLICATION

? ?Mechanistic Evaluation of Virus Clearance by Depth Filtration? ? Biotechnol. Prog, 2015, PMID: 25683459

? ?High AU content: A Signature of Unregulated miRNA in Cardiac Diseases? ? BIOINFORMATION, 2010, PMID: 21364793

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