Barbara Henson

**** ******* *****

San Antonio, Texas 78254

Cell Phone: 210-***-****

E-mail: acyf44@r.postjobfree.com

EDUCATION: University of Texas Health Science Center, San Antonio, TX

M.S. Forensic Toxicology (Thesis-Based) May 20, 2007

Schreiner University, Kerrville, TX

B.S. Biochemistry May 5, 2002

Relevant coursework:

Biochemistry Genetics Microbiology

Organic Chemistry Cell Biology Medical

Terminology

Physical Chemistry Analytical Chemistry Anatomy &

Physiology

Forensic Toxicology Clinical Chemistry

Pharmacology

Honors/Awards:

Chancellors List for Graduate School, 2004-2006

President's List and Dean's List every semester

Hill Country Scholarship, 2000-2002

Johnny R. Davis Scholarship, 1998-2002

Outstanding Workstudy, 2002

WORK EXPERIENCE:

Lab Manager/Toxicologist: LSASA, LLC, San Antonio, TX, May 2015-October

2016.

Duties: Responsible for technical and scientific oversight of the

laboratory.

> Selects test methodologies appropriate for the clinical use of the

test menu

> Verifies test procedures performed and establishes the laboratory's

performance criteria, including accuracy and precision of each test

and test system

> Enrolls the laboratory in an approved PT program commensurate with

services offered

> Establishes a quality control program appropriate for the testing

performed

> Establishes the acceptable levels of analytic performance, and ensures

these levels are maintained throughout the testing process

> Resolves technical problems and ensures remedial actions are taken

whenever test systems deviate from the laboratory's established

performance specifications

> Ensures patient test results are not reported until all corrective

action has been taken and the test system is functioning properly

> Identifies training needs and ensures testing personnel receive

regular in-service training

> Evaluates the competency of all testing personnel on an ongoing basis

> Evaluates and documents performance of individuals responsible for

testing at six months and twelve months in the first year of

employment and yearly thereafter, unless test methodology or

instrumentation changes, in which case, prior to reporting patient

test results, the individual's performance must be reevaluated for the

new test methodology or instrumentation

> Answers doctors/medical assistant's questions concerning drugs and

metabolites and/or general toxicology questions

> Correspond with sales reps on incorporating new tests and educating

them on toxicology and drug metabolism

> Toxicology sales and support for pain management, family practice and

treatment centers.

Lab Manager/Toxicologist: Frontier Toxicology, San Antonio, TX, February

2014-April 2015.

Duties: Responsible for technical and scientific oversight of the

laboratory, answering doctor's phone calls in regards to patient toxicology

results and overall management of the laboratory.

Lab Manager (Pharmacogenomics Lab): Frontier Toxicology, San Antonio, TX,

January 2014-September 2014.

Duties: Responsible for technical and scientific oversight of the

laboratory

> Started up the Pharmacogenomic (PGx) laboratory at Frontier Toxicology

> Review reports and communicate with doctors about drug metabolism and

drug-drug interactions

Analyst II/Toxicologist: Hill Country Toxicology, San Antonio, TX, May

2013-January 2014.

Duties: Performs laboratory tests, procedures, experiments and analyses to

provide data as specified or required in toxicological evaluation of

patient samples. Communicated patient results and interpretations of

toxicological findings to doctors and/or medical staff. Reviewed all data

for QA/QC issues.

Toxicology Chemist: Bexar County Medical Examiner's Office, San Antonio,

TX, August 2008-May 2013.

Duties: Responsible for performing toxicological analyses on cases

submitted to the Medical examiners office and other functions as required

by the QA Officer, a Senior Toxicology Chemist or the Chief Toxicologist.

Research Assistant: Department of Pediatrics, Division of Neonatology,

University of Texas Health Science Center, San Antonio, TX, September 2002-

August 2008.

Duties: Carry out molecular and biological techniques, such as, Western and

Northern blots, cell culture, protein assays, isolation and quantitation of

RNA, immunofluorescence, protein from whole tissue and cells, bacterial

transformations, transfections, and RT-PCR. Assist several pediatric

fellows and neonatologists with laboratory and clinical based research

projects.

Work-Study Program: Biology Department, Schreiner University, Kerrville,

TX, August 1998-May 2002.

Duties: To help set up science labs, prepare solutions, clean glassware,

inventory of chemicals, and assist students in lab processes.

Alpha Diagnostics Int., Inc: Research/Sales Company, San Antonio, TX,

Summer 2000 and 2001.

Duties: Antibody analysis by ELISA assay, coupling proteins to sepharose,

affinity purification of antibodies, and electrophoresis of brain and

digestive proteins.

INTERNSHIP:

Forensic Toxicology Lab: Bexar County Forensic Toxicology Lab, San Antonio,

TX, Summer 2006.

Duties: Analyzed drugs and poisons in human tissue and body fluids,

including blood alcohol results. Performed acid/neutral and alkaline drug

analysis and screening using GC and GC/MS, as well as, cocaine/opiates,

THC, enzymatic assays, alcohols, accessioning, and Conway diffusion.

SKILLS:

LABORATORY:

Chromatography: paper and thin layer Bacterial

culturing

Electrophoresis: SDS-PAGE and agarose Autoclaving

Spectroscopy Micropipeting

Purification of antibodies

ELISA assays

Protein Assays Cell Culture

DNA/RNA Tissue explants

Animal Handling/Surgery Microscopy

Immunofluorescence Bacterial

Transformations

Prepare solutions/media Transfections

GC/FID and GC/MSD RT-PCR

TOXI-LAB LC/MS/MS

Head-Space GC QA/QC Verification

COMPUTERS:

Microsoft Office, Spreadsheet Applications, Computer programming

(Visual Basic), Front Page, Printshop Pro, Website creation, and

Internet experience.

OTHER:

IRB writing and documentation procedures (based on clinical

research)

Writing/Reviewing SOP's

Compliance protocols/guidelines

QA/QC guidelines

ACTIVITIES:

Science Club (1999-2002)

Science Club President (2001-2002)

Expanding Your Horizons (1998-2003)

Schreiner University Science Camp (1999, 2000)

Student Senate Science Club Representative (2001-2002)

Texas Academy of Science (1999-2002)

UTHSCSA Safety Evacuation Committee (2005-2008)

Southwestern Association of Toxicologists (SAT) Fall 2008 Meeting, Austin,

TX (December 2008)

SAT Spring 2009 Meeting, San Antonio, TX (April-May 2009)

SAT Spring 2012 Meeting, San Antonio, TX (May 2012)

PROFESSIONAL ASSOCIATION MEMBERSHIPS:

Texas Academy of Science member (1999-2002)

American Association of University Women (2002-2003)

SAT Member (2009-present)

SAT Vendor Liaison (2012-2014)

TEACHING:

2014-present Adjunct Assistant Professor, UTHSCSA, Clinical Laboratory

Sciences, School of Health Professions

Neonatal Subspecialty Residents

2002-2005 Dr. Johnson Isaac, M.D., Wilford Hall Medical Center. Finalist

for The Young Investigator Award in Basic Science, The Southern

Society for Pediatric Research, 2005. Manuscript titled

"Mechanical stretch upregulates ?-epithelial Na+ channel (?-

ENaC) expression in murine lung epithelial (MLE-12) cells via

MAPK activation." To be Submitted.

2004-2005 Dr. Ryan McAdams, M.D., Wilford Hall Medical Center.

Manuscript titled "Cyclic stretch attenuates the effects of

hyperoxia on cell proliferation and viability in human alveolar

epithelial cells". American Journal of Physiology, Lung

Cellular and Molecular Physiology, 291, L166-L174, 2006.

2003-2006 Dr. Margarita Vasquez, M.D., UTHSCSA. Finalist for The

Young Investigator Award in Basic Science, The Southern Society

for Pediatric Research, 2006. Manuscript titled "Induction of

serum and glucocorticoid-induced protein kinase-1 (sgk-1) and

epithelial sodium channels (ENaC) by cAMP in epithelial cells".

2004-2007 Dr. Michael Shoemaker, Ph.D., M.D., Wilford Hall Medical

Center. Project title "Effects of hyperoxia on ENaC expression

in murine lung epithelial cells".

2005-2008 Dr. Chris Bishop, M.D., UTHSCSA. Project title "The Effects of

IL-1 beta on Glucocorticoid Receptor Expression in MLE-12

Cells".

2005-2008 Dr. Tim Biela, M.D., UTHSCSA. Project title "Regulation of

Epithelial Sodium Channels by Synthetic Glucocorticoids in MLE-

12 Cells".

2007-2008 Dr. Amy Fowler, M.D., UTHSCSA.

2007-2008 Dr. Aaron Reeves, M.D., UTHSCSA. Project title "Trafficking

and Function of the Epithelial Sodium Channel Is Inhibited By

Epidermal Growth Factor In A Rat Parotid Gland Cell Line".

2007-2008 Dr. Taslim Allibhai, M.D., Wilford Hall Medical Center.

2007-2008 Dr. Kelsey Sherburne, M.D., UTHSCSA. Project title "Maternal

Human Milk versus Donor Human Milk: an in vitro study on

cytokine production and lactoferrin levels".

2007-2008 Dr. Tania Hernandez, M.D., UTHSCSA. Project title "IL-1

Transiently Increases Epithelial

Sodium Channel Expression in Lung Epithelial Cells.

Graduate

2015 Salina Vargas, UTHSCA, MS candidate.

2014 Richard Mazuca, UTHSCA, MS candidate. Project title "The

Colorectal Carcinogenic

Potential of Ingested Aloe barbadensis Miller's

Anthraquinones and Anthrones.

2013 Victor Trejo, UTHSCA, MS candidate. Project title

"Physiological similarities between the

use of Synthetic Stimulants "Bath Salts" Cocaine, Ecstasy, and

Methamphetamine.

2008 Madhu Jupelli, UTSA, Ph.D. candidate. Project title "The mechanisms

involved in development of airway hyper-reactivity in adult

mouse following neonatal pulmonary Chlamydia infection".

Undergraduate

2003-2004 Haleema Harhara, St. Mary's University, volunteer summer

student.

2004 Supervision and teaching Christa Niemoller-Kincaid as part of an

internship in collaboration with Schreiner University, TX.

2005 Sarah Yoder, Rice University, volunteer summer student.

2005 Ashaini Kadakia, UT Austin, volunteer summer student.

2005-2006 Thesis Mentor for Senior UTSA Honors Student: Ana Nicho.

Project title "Disruption of the cytoskeletal network alters

Epithelial Na+ Channel (ENaC) activity".

2006 Aasya Nasar, UT Austin, volunteer summer student.

2006-2007 Jonathan Rodriguez, UTSA, volunteer.

High School

2002-2003 Mentor for a Health Careers High School Student as part of The

Independent Student Mentorship Program: Kinza Shamsi, Project

title "The Effect of Cytokines on Sodium Channel Protein

Expression in Mouse Lung Epithelial Cells."

2003 Aasya Nasar, volunteer summer student.

2004 Ryan DiGeronimo, volunteer summer student.

2005-2006 Mentor for a Health Careers High School Student as part of The

Independent Student Mentorship Program: Chelsea Rodriguez,

Project title "Research in the Division of Neonatology."

PUBLICATIONS:

Abstracts

1. J.A. Petershack, S.R. Seidner, B.M. Henson, and S.B. Mustafa.

Regulation of ? -Epithelial Sodium Channels (?-ENaC) by Interleukin-1 in

Murine Lung Epithelial (MLE-12) Cells. J. Invest. Med, 51, S92, 2003.

2. J.A. Petershack, S.R. Seidner, R. Castro, B.M. Henson, A.J. Falck, and

S.B. Mustafa. Stimulation of ? -Epithelial Sodium Channels by DibucAMP in

Epithelial Cells: Role of MAP Kinase Pathways. Ped. Res. 53, (4), 429A,

2003.

3. J. Isaac, R. J. DiGeronimo, B.M. Henson, C.E. Bishop, S.R. Seidner, and

S.B. Mustafa. Mechanical Stretch regulates ? -Epithelial Na+ Channel

Expression in Murine Lung (MLE-12) Epithelial Cells: Role of MAPK Pathways.

Am. J. of Resp. and Crit. Care Med., 2004.

4. M.M. Vasquez, R. Castro, B.M. Henson, T. A. Biela, S.R. Seidner, and

S.B. Mustafa. cAMP-Dependent Regulation of ?-ENaC in submandibular Gland

Epithelial Cells: Possible Crosstalk Between PKA and PI3K Signaling

Pathways. J. Invest. Med. 52, S297, 2004.

5. M.M. Vasquez, R. Castro, S.R. Seidner, J.A. Petershack, B.M. Henson,

T.A. Biela, S.B. Mustafa. cAMP Regulation of ?-ENaC in Epithelial Cells

Involves Activation of the PI3-Kinase pathway: Role of Serum and

Glucocorticoid-Induced Protein Kinase (sgk1). Ped. Res. 55, 449A, 2004.

6. C.L. Blanco, J.A. Petershack, S.B. Mustafa, B.M. Henson, R.I. Clyman,

S.R. Seidner. Ibuprofen increases ?-Epithelial Sodium Channel Protein in

Preterm Baboons. Ped. Res. 55, 449A, 2004.

7. J. Isaac, R.J. DiGeronimo, P.S. Dixon, B.M. Henson, C.E. Bishop, A.S.

Nasar, S.B. Mustafa. Mechanical Stretch Regulates ?-Epithelial Sodium

Channel (?-ENaC) Expression in Murine Lung Epithelial (MLE-12) Cells. J.

Resp. and Crit. Care Med. 169, A737, 2004.

8. J.M. King, S.B. Mustafa, P.S. Dixon, B.M. Henson, R.J. DiGeronimo.

Effect of Cyclic Stretch on Tight Junction Protein Expression in Mouse Lung

Epithelial (MLE-12) Cells. A787, 2004.

9. R.M. McAdams, P.S. Dixon, S.B. Mustafa, B.M. Henson, R.J. DiGeronimo.

Cyclic stretch attenuates the effects of hyperoxia on cell proliferation

and viability in pulmonary epithelial A549 cells. J. Invest. Med 53, S292,

2005.

10. J. Isaac, R. J. DiGeronimo, B.M. Henson, P. Dixon, S.R. Seidner, and

S.B. Mustafa. Mechanical Stretch stimulates ?-Epithelial Na+ Channel

Expression in Murine Lung Epithelial Cells: Role of MAPKS. J. Invest. Med.

53, S292, 2005.

11. T.A. Biela, B.M. Henson, S.R. Seidner, R. Castro, and S.B. Mustafa.

Synthetic Glucocorticoids (GC) Selective for Transactivation stimulate ?-

ENaC Formation but do not Suppress Expression of Glucocorticoid Receptors

(GCR) in Mouse Lung Epithelial (MLE-12) Cells. Ped. Res. 2005.

12. M.M. Vasquez, R. Castro, S.R. Seidner, B.M. Henson, D.J. Ashton, and

S.B. Mustafa. Induction of Serum and Glucocorticoid Induced Protein Kinase-

1 (sgk1) and Epithelial Sodium Channels (ENaC) by cAMP in Epithelial Cells.

J. Invest. Med., 54 (1), S 280, 2006.

13. A.A. Reeves, S.B. Mustafa, B.M. Henson, M.M. Vasquez, and R. Castro.

Trafficking and Function of the Epithelial Sodium Channel Is Inhibited By

Epidermal Growth Factor In A Rat Parotid Gland Cell Line. J. Invest. Med.,

Jan 2007.

14. T.A. Biela, S.R. Seidner, B.M. Henson, and S.B. Mustafa. Regulation of

epithelial sodium channels and glucocorticoid receptors by glucocorticoids

selective for transactivation. J. of Invest. Med., Jan 2008.

15. T.F. Hernandez, T.A. Biela, J.A. Petershack, S.R. Seidner, B.M. Henson,

and S.B. Mustafa. IL-1 transiently increases epithelial sodium channel

expression in lung epithelial cells. Ped. Res., Jan 2008.

Papers

1. J. A. Petershack, B.M. Henson, D. J. Ashton, S.R. Seidner, and S.B.

Mustafa. Regulation of ? -Epithelial Sodium Channels by Interleukin-1 in

Murine Lung Epithelial (MLE-12) Cells. (Manuscript in Preparation).

2. R.M. McAdams, S.B. Mustafa, J.S. Shenberger, P.S. Dixon, B.M. Henson,

and R.J. DiGeronimo. Cyclic stretch attenuates the effects of hyperoxia on

cell proliferation and viability in human alveolar epithelial cells.

American Journal of Physiology, Lung Cellular and Molecular Physiology,

291, L166-L174, 2006.

3. S.B. Mustafa, R. Castro, A.J. Falck, J.A. Petershack, B.M. Henson, Y.M.

Mendoza, M.A. Choudary, and S.R. Seidner. Protein Kinase A and Mitogen-

Activated Protein Kinase Pathways Mediate cAMP Induction of ?-Epithelial

Na+ Channels (?-ENaC). Journal of Cellular Physiology, 215(1): 101-10,

2008.

4. M.M. Vasquez, R. Castro, S.R. Seidner, B.M. Henson, D.J. Ashton, and

S.B. Mustafa. Induction of serum- and glucocorticoid-induced kinase-1

(SGK1) by cAMP regulates increases in ?-ENaC. In Press, Journal of

Cellular Physiology, 2008.

5. S.B. Mustafa, J. Isaac, S.R. Seidener, P.S. Dixon, B.M. Henson, and

R.J. DiGeronimo. Mechanical Stretch induces lung ?-Epithelial Na+ Channel

Expression, Experimental Lung Research, 2014.

REFERENCES AVAILABLE UPON REQUEST.

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