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Biomedical Research

Location:
Silver Spring, Maryland, United States
Salary:
35,000/yr
Posted:
October 31, 2016

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Babita Parajuli

*** ******** **, ****** ****, MD 20912

acxbpl@r.postjobfree.com -240-***-****

SUMMARY:

Highly self-motivated graduate with significant experience working in fast-paced laboratories

Continually uphold a certification of accuracy in microbiology.

Strong knowledge of analytical techniques like PCR, RT-PCR, Western Blotting, Mammalian Cell Culture, Vector Construction, gene cloning.

EDUCATION:

University of Maryland, College Park, MD May 2016

B.S in Biochemistry

Montgomery College, Takoma Park, MD Jun2012 - May 2014

A.S in Chemistry and Biochemistry

Relevant Coursework:

Biochemistry I, II, III, Biochemistry Lab, Calculus I, II, III, Physical Chemistry, Organic Chemistry, Instrumental Analysis, Statistics, Anatomy and Physiology I, II, Molecular Genetics, Virology, and more.

RESEARCH EXPERIENCE:

Jay Research Group

Undergraduate Researcher, University of Maryland Aug 2015 – Aug 2016

Job Responsibilities:

Synergized molecular characteristics of Extracellular Vesicles with nanotechnology approaches to engineer novel nanobiotherapeutics for multiple applications

Performed molecular cloning through RNA isolation, RT-PCR, PCR, restriction enzyme digests, cell transformation

Developed in-vitro assays for extracellular vesicles studies, including cell migration and proliferation assays

Performed Immunoblot Assays, SDS PAGE, agarose gel electrophoresis, mammalian cell culture

Isolated and analyzed extracellular vesicles, RNA and protein

Loaded adjuvants, such as R848 and CPG into EVs for vaccine development project

Labeled EVs with siRNA, ssDNA & miRNA

Instrumentation knowledge including UV-Vis Spectrophotometer, HPLC, ELISA, Gas Chromatography, Fluorescence plate reader

Data collection and analysis

Updated and maintained laboratory databases and notebooks

Thorough understanding of sterilization techniques and handling of harmful materials

Knowledge of using, maintaining and cleaning (autoclaving/dish washing) laboratory equipment

Presented the findings on weekly meeting

Undergraduate Researcher, University of Maryland Jan 2014 - Sept 2015

The First- Year Innovation and Research Experience

Job Responsibilities:

Independent research on identifying the sources of pathogens in environmental water

Total DNA and RNA extraction and purification, gel electrophoresis, PCR

Designed primers

Maintained clear, accurate and original laboratory data

Excellent skills in MS Word, PowerPoint and Excel

Cashier, McDonalds Jun 2012-Sept 2013

Job Responsibilities:

Handled cash and credit card transactions

Took customer orders

Maintained good attitude with customers and other team members

Tutor – Montgomery Blair High School, Silver Spring, MD Sept 2010 - Nov 2015

Job Responsibilities:

Helped 4-5 students daily in science and math

Developed strong personal relationship with other students

Student Assistant - Rolling Terrace Elementary School, Takoma Park, MD May-Aug 2014

Tutored 4th grade students in science and math

Organized informational and entertainment events such as PTA Day

Assisted teacher with grading

AFFILIATIONS

Active member of American Chemical Society

Active member of Nepalese Student Association (NSA)

President of Global Culture Club

Volunteering Experience

Performed scientific experiments at Maryland Science Olympiad and Maryland Day

Taught science and math to middle and high school kids

Packed food for homeless

PUBLICATION:

Tek N. Lamichhane, Anjana Jeyaram, Divya Patel, Babita Parajuli, Natalie Livingston, Navein Arumugasaamy, John Schardt and Steven M. Jay. Oncogene Knockdown via Active Loading of small RNAs into Extracellular Vesicles by Sonication (Accepted and In Press in Cellular and Molecular Bioengineering Journal)

PRESENTATION:

“Delivery of Negatively Charged Therapeutic Molecules into Extracellular Vesicles Prepared by pH-Gradient Approach” Undergraduate Research Day, University of Maryland, College Park, MD, April 2016.



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