Quality Microsoft Office
Resume:
Stephen Werner
*********@*****.*** - 720-***-****
Work Experience
Analytical Chemist
US Geological Survey, Water Resources Div., National Water Quality Laboratory
Lakewood, CO
June 1985 to March 2016
I worked for the US Geological Survey, Water Resources Division, National Water Quality Laboratory from June 1985 until March 2016.
During that time, I have worked with numerous analytical techniques and state-of-the-art instrumentation/data system. My primary focus has been High Performance Liquid Chromatography & Mass Spectroscopy, using a variety of HPLC/Mass Spectroscopy instrumentation, including tandem mass spectroscopy, ion trap mass spectroscopy, and time-of-flight mass spectroscopy and the associated instrument control and data analysis software packages.
I also have experience with Gas Chromatography & Mass spectroscopy, Inductively-Coupled Plasma spectroscopy, Cold-Vapor Mercury analysis, and multiple sample preparation techniques.
The majority of my time at the NWQL, I have been with the Methods Research & Development Program, and have been involved in numerous method development projects and scientific publications.
I have been involved in the development of methodology with the multiple objectives of providing accurate identification and quantitative of diverse environmental contaminates including pesticide compounds, and emerging contaminates such as pharmaceutical and human personal care product wastes; incorporating advancements in sample preparation for improved performance, reduction in chemical usage, and automation; and to allow for the use of increasing advanced and sensitive instrumentation to achieve more sensitive and more reliable results.
Developed solid phase extraction and instrumental analysis methods for pesticides by High Performance Liquid Chromatography (HPLC)/Photo diode-array, and by HPLC/Mass Spectroscopy (MS); developed methods for solid phase extraction, solvent extraction of solid samples, and instrumental analysis of pharmaceutical and human use personal care product wastes by HPLC/MS & HPLC/tandem Mass Spectroscopy (MS/MS)
Developed methods for the utilization of UPLC/Time-of-Flight mass spectroscopy for accurate mass identification of contaminants and structural elucidation of unknown chemical species in samples.
Analyzed various sample types in support of scientific publications in collaboration with others.
Assisted and trained graduate students, post-doctoral fellows, and international visiting scientists on projects involving sample preparation and HPLC/MS, HPLC/MS/MS, and UPLC/Time-of-Flight Mass Spectroscopy.
Education
BA in Molecular, Cellular, and Developmental Biology
University of Colorado, Boulder -
Boulder, CO
1981 to 1986
Skills
Agilent 6420 & 6465 tandem mass spectrometers with Mass Hunter software (7 years),
Waters Synapt-G2S time-of-flight mass spectrometer with Mass Lynx and UniFi software (3 years),
ABI-Sciex Q-Trap 2000 linear ion-trap tandem mass spectrometer with Analyst software (7 years),
Agilent 1100 HPLC-MS systems with ChemStation Software (10+ years),
Dionex ASE solvent extraction instrumentation (7 years),
Spark-Holland Prospect 2 and Symbiosis sample on-line Solid Phase Extraction instrumentation and automation software (2 years),
Solid-phase liquid extraction techniques, both manual and automated (10+ years),
Perkin-Elmer 3400 ICP-OES systems (2 years), Bruker Ion-Trap LC/MS (3 years),
Sample Chain Of Custody (3 years),
Assisting with NELAC Certification (5 years), Microsoft Office (10+ years),
Laboratory Information Managament System (LIMS) (10+ years),
Technical scientific writing and publication (10+ years),
Laboratory layout and equipment installation (10+ years)
References
Edward T. Furlong, Project Chief, Ph.D., Chemical Oceanography, USGS National Water Quality Laboratory, Building 95 Denver Federal Center, Lakewood CO, 800225
********@****.*** 303-***-**** (http://nwql.usgs.gov/nwql.shtml)
William T. Foreman, Project Chief, Ph.D., Analytical Chemistry, USGS National Water Quality Laboratory, Building 95 Denver Federal Center, Lakewood CO, 800225
********@****.*** 303-***-****
Jeff W. McCoy, Program Chief, PhD., Analytical Chemistry, USGS National Water Quality Laboratory, Building 95 Denver Federal Center, Lakewood CO, 800225
********@****.*** 303-***-****
Lisa Bressler, Data Quality Specialist, USGS National Water Quality Laboratory
Building 95 Denver Federal Center, Lakewood CO, 800225
********@****.*** 303-***-****
Publications
Werner, S.L., Burkhardt, M.R., and DeRusseau, S.N., 1996, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory--Determination of Pesticides in Water by Carbopak-B Solid-Phase Extraction and High-Performance Liquid Chromatography: U.S. Geological Survey Open-File Report 96-216, 42 p. - http://nwql.usgs.gov/rpt.shtml?OFR-96-216
Werner, S.L., and Johnson, S.M., 1994, Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory--Determination of selected carbamate pesticides in water by high-performance liquid chromatography: U.S. Geological Survey Open-File Report 93-650, 29 p. 1994
Furlong, E.T., Burkhardt, M.R., Gates, P.M., Werner, S.L., and Battaglin, W.A., 2000, Routine determination of sulfonylurea, imidazolinone, and sulfonamide herbicides at nanogram-per-liter concentrations by solid-phase extraction and liquid chromatography/mass spectrometry: Science of the Total Environment, v. 248, p. 13-146.
Furlong, E.T., Anderson, B.D., Werner, S.L., Soliven, P.P., Coffey, L.J., and Burkhardt, M.R., 2001, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory---Determination of Pesticides in Water by Graphitized Carbon-Based Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass Spectrometry: U.S. Geological Survey Water-Resources Investigations Report 01-4134, 73 p. - http://nwql.usgs.gov/rpt.shtml?WRIR-01-4134
Furlong, E.T., Werner, S.L., Anderson, B.D., and Cahill, J. D., 2008, Determination of human-health pharmaceuticals in filtered water by chemically modified styrene-divinylbenzene resin-based solid-phase extraction and high-performance liquid chromatography/mass spectrometry: U.S. Geological Survey Techniques and Methods, book 5, sec. B, chap. B5,
56p. - http://nwql.usgs.gov/rpt.shtml?TM-5-B5
Furlong, E.T., Gray, J.L., Quanrud, D.M., Teske, S.S., Werner, S.L., Esposito, Kathleen, Marine, Jeremy, Ela, W.P., Zaugg, S.D., Phillips, P.J., and Stinson, Beverley, 2012, Pharmaceuticals, hormones, anthropogenic waste indicators, and total estrogenicity in liquid and solid samples from municipal sludge stabilization and dewatering: U.S. Geological
Survey Open-File Report 2011-1132, 8 p. - http://pubs.usgs.gov/of/2011/1132
Kinney, C.A., Furlong, E.T., Kolpin, D.W., Burkhardt, M.R., Zaugg, S.D., Werner, S.L., Bossio, J.P., and Benotti, M.J., 2008, Bioaccumulation of Pharmaceuticals and Other Anthropogenic Waste Indicators in Earthworms from Agricultural Soil Amended with Biosolid or Swine Manure: Environmental Science and Technology. - http://nwql.usgs.gov/rpt.shtml?EST-08-01
Schultz, M.M., Painter, M.M., Bartell, S.E., Logue, A., Furlong, E.T., Werner, S.L., Schoenfuss, H.L., 2011, Selective uptake and biological consequences of environmentally relevant antidepressant pharmaceutical exposures on male fathead minnows: Elsevier, Aquatic Toxicology 104 (2011) p. 38-47 - http://nwql.usgs.gov/rpt.shtml?AT104_38-47
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