Rameshwari Rayaji

San Jose, CA 201-***-**** acx9h4@r.postjobfree.com

SUMMARY Motivated, achievement-driven and goal-oriented professional seeking full time opportunities in research and development, tissue and genetic engineering, quality assurance and allied areas.

EDUCATION

Master of Science, Biomedical Engineering, Rutgers University, New Brunswick GPA: 3.4 Sep 2016

Courses: Biosignal & Image Processing, Artificial Implants, Stem Cell Dynamics, Biocontrol Modeling & Computation,

Innovation & Entrepreneurship for Science and Technology, Biomechanics, Microfluidics.

Bachelor of Engineering, Biotechnology, B.V.B CET, India GPA: 9.26 (Gold Medalist) Jun 2012

Courses: Cell & Molecular Biology, Bioinformatics, Biochemical Analytical Techniques, Bioprocess Technology, Economics & Plant Design, Enzyme Technology, Process Control & Instrumentation, Genetic Engineering.

SKILLS

Experimental: Mammalian cell & explant culture, Protein isolation and characterization, PCR, qPCR, Immunofluorescence assay, Bench scale bioreactor, Fermentation, Upstream & downstream processing, HPLC, GC, MS, ELISA, Aseptic techniques, Sterile surgical procedures, Embryonic injection, Maintain KO mouse line, Cell and Molecular biology techniques, Nanoparticle synthesis, Image segmentation, GxP.

Technical: MATLAB, Minitab, Labview, C, C++, Data Structures, Java-J2EE.

RESEARCH PRESENTATIONS /PUBLICATIONS

● Secured first place in oral presentation on ?Production, Purification and Characterization of Fungal Lipases for Potential Application in Biodiesel Production and Detergent Industry? at International Conference on Exploration of Biotechnology by Students Scholars and Researchers, held at SIET, Tumkur, India.

● Published the same in Acta Biologica Indica 2013, 2(1):367-377, Association for the Advancement of Biodiversity Science.

EXPERIENCE

Research Lab Tech I- Biomedical Engineering, Rutgers Jun 2015 ?Oct 2016

● Designed and structured tissue engineering and gene transformation lab.

● Performed genotyping, qPCR, vector construction, shRNA design and bacterial transformation.

● Carried out topoisomerase 2b knockdown using Cre plasmid isolation, purification, followed by electroporation.

● Established a new Topoisomerase 2b knockout mouse line by using the Cre/LoxP system in selected tissues.

Graduate Researcher- Thesis Project Li Cai Lab, Rutgers Mar 2015 ? Sep 2016

● DOE for ?Effect of Topoisomerase 2b Inhibition on Cadherin Expression in Developing Chicken Retina?.

● Carried out retinal embryonic injections to inhibit Topoisomerase 2b using inhibitor ICRF-193.

● Analyzed inhibition results using immuno-histochemistry, qPCR and SDS-PAGE.

● Reduced cadherin expression leading to neuropsychiatric disorders addressed by Topoisomerase 2b inhibition.

Clinical Work Shadow-Robert Wood Johnson Hospital Dec 2014 ? Jan 2015

● Shadowed surgeons in operation theatres, participated in robotic/instrumentation workshops and ambulatory care.

● Administered and advised technical solutions to problems faced by doctors.

Lab Assistant- Shreedhar Bhat?s Laboratory, India May 2010 ? Sep 2010

● Trained students to perform recombinant DNA technology, qPCR, plant tissue culture, genotyping, immunology.

Part Time Lecturer- Computer Science, Rutgers University Sep 2015 ? May 2016

● Delivered lectures on introduction to computer applications and scratch programming, graphics and webpage-design.

Guest Lecturer- OSAP, Robert Wood Johnson Medical School Jun 2015? 16-Jul 2015-16

● Delivered lectures on organic chemistry, thermodynamics and kinetics, stereochemistry.

Research Analyst- Tata Consultancy Services Oct 2012 ? Jun 2014

● Performed testing of TCS-BaNCs, delivered sales pitch, product demo, on boarded international clients.

ACADEMIC PROJECTS

● Designed and developed prototype hand held scanner using harmonic impulse excitation to detect tissue properties.

Dec 2014-Sep2015

● REU: Produced biodiesel from Pongamia Pinnata oil implementing enzymatic transesterification. Evaluated role of antioxidants to maintain biodiesel stability, project funded by Indian Institute of Science. Jun 2011-Jul 2012

● Isolation, Purification and Screening of Actinomycetes for secondary metabolites. Jun 2010-May 2011

● Design for effective degradation of textile dyes by microorganisms isolated from activated sludge. 2009-2010

ACHIEVEMENTS

● Presented poster on "Role of Topoisomerase IIb in Neural Development? at JMBGSA, Rutgers symposium, 2016.

● Secured first place in oral presentation at National Symposium BIOSATUM, BIET Davangere, India, 2012.

● Organized workshop on ?HPLC an Analytical Tool? at National fest- Pleiades, BVBCET, India, 2012.

● Vice President Neo-BT organization of Biotechnology Department, BVBCET, 2010-2012.

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