Medical Research

Liwei Chen

MB (ASCP)/PhD

Wesley Chapel, FL *****

********@*****.*** - 813-***-****

Work Experience

Research Scientist

Moffitt Cancer Center

-Tampa, FL

April 2016 to present

Responsibilities

Discovery of small molecules (disruptors) inhibiting the interaction of KRas and Raf.

Skills Used

High throughput screening of large chemical libraries, Isothermal Titrating

Calorimetry (ITC), BiaCore T100 SPR system, AlphaScreen technique, PCR, Real-time PCR, Southern blotting, Northern blotting, and Western blotting;

Cell culture, DNA and RNA extraction and purification from cells and tissues, genotyping;

Biological Scientist

University of South Florida

-Tampa, FL

February 2012 to April 2016

Responsibilities

Developed and optimized the ECIS (Electric cell-substrate Impedance Sensing) system; Actively involved in projects on intestinal barrier dysfunction.

Skills Used

PCR, Real-time PCR, Southern blotting, Northern blotting, and Western blotting;

Cell culture, DNA and RNA extraction and purification from cells and tissues, genotyping;

Animal manipulation, models establishment and transgenic techniques: Animal Surgery: mouse/rat Jugular vein catheterization; mouse laparoscopy

Postdoctoral Fellow

Moffitt Cancer Center

-Tampa, FL

April 2004 to November 2011

Responsibilities

Actively involved in P01 Cancer Drug discovery project “Targeting Signal Transduction Pathways for Cancer Drug Discovery 1P01CA118210-01A1” (one of the biggest funding support from NCI in the history of Moffitt Cancer Center).

Plan and direct studies to investigate human cancer, preventive methods, and treatments for disease.

Study animal and human health and physiological processes.

Prepare and analyze organ, tissue, and cell samples to identify signal pathway of target molecules.

Evaluate effects of drugs on inhibiting oncogene targets at various levels.

Write and publish articles in scientific journals.

Supervised and trained graduate students and summer interns to inspire them to make peak performance and teaches them all kinds of basic biological lab techniques.

Regularly order, receive, aliquot, and properly store purchases shipped to the lab.

Conduct research to develop methodologies, instrumentation, and procedures for medical application, analyzing data and presenting findings to the scientific audience and general public.

Accomplishments

First authorship in 4 papers published in peer-reviewed journals with 126, 87, 34 and 11 citations, respectively;

One of major contributors to 2 patents: Inhibition of Shp2/PTPN11 Protein Tyrosine Phosphatase by NSC-87877, NSC-117199 and Their Analogs, US20080176309 A1; INDOLINE SCAFFOLD SHP-2 INHIBITORS AND METHOD OF TREATING CANCER, WO 2,010,121,212

Skills Used

Molecular biological techniques: primer design, gene cloning, sequencing (NGS, pyrosequencing), site-mutagenesis, etc. Experienced in PCR, RT-PCR, and real time quantitative PCR experiments on RNA isolated from cultured cells and frozen tissue;

Biochemical techniques: spectrophotometer, protein extraction and purification, Western blotting, and immunoassay platforms such as ELISA, Luminex;

Cellular biology techniques: cell culture, confocal microscopy, fluorescent microscopy (FISH assay), flow cytometry analysis (FACs).

Animal manipulation, models establishment and transgenic techniques: Xenograft tumor mouse model; myc knock out mouse model; Shp2E76K mutant knock-in mouse model; Retrovirus and lentivirus systems for overexpression and/or knock-down cell lines establishment; Animal Surgery: mouse/rat Jugular vein catheterization; mouse laparoscopy

Postdoctoral Fellow

Shriners Hospital for Children Portland Research Center

-Portland, OR

August 2003 to March 2004

Education

Ph.D in Pharmacology

Shanghai Institute of Materia Medica, Chinese Academy of Sciences – Shanghai, China

1999 to 2002

M.Sc in Pharmacology

Xiangya School of Medicine, Central South University -

Changsha, China

1995 to 1998

DDS in Dentistry

Xiangya School of Medicine Central South University -

Changsha, China

1990 to 1995

Additional Information

Licensureship:

Clinical Laboratory Technologist

TN 46877

02/14/2015 to 08/31/2016

Professional Affiliations:

• American Society for Clinical Pathology

• American Association for Cancer Research (AACR), U.S.A, 2005-present

• Chinese Pharmacological Society, China, 1997-1999

• Chinese Section, International Society for Heart Research (ISHR), 1997-2001

• Hunan Physiological Society, China, 1996-1999

Awards & Honors:

• Outstanding Poster Winner of 2010 Moffitt Scientific Retreat.

• Second and Third Scholarship of Hunan Medical University, China, 1991-1994

• Second Prize of “Di-Ao Excellent Graduate Award”, Chinese Academy of Sciences, 2001-2002

Poster Presentations:

• SPI-112Me, a new Shp2 inhibitor, exhibits strong anti-tumor activity. Moffitt Annual Scientific Retreat, 2010

• Departmental Seminar – “Discovery of Novel Shp2 Inhibitors: identification of potent Shp2 inhibitors”. Research in Progress of Molecular Oncology at Moffitt, 2005

• Chemical ablation of androgen receptor in prostate cancer cells by the histone deacetylase inhibitor LAQ824. 96th AACR Conference minisymposium on "Experimental and Molecular Therapeutics”. 2005

• China Zhangjiajie/1998 China-Japan Joint Meeting on Pharmacology

• China Changsha/1998 Hunan Physiological Conference

Peer-Reviewed Publications:

1. Maschek AJ, Mevers E, Diyabalange T, Chen L, Ren Y, McClintock JB, Amsler CD,

Wu J and Baker BJ. Palmadorin chemodiversity from the Antarctic nudibranch Austrodoris kerguelenensis and inhibition of Jak2/STAT5-dependent HEL leukemia.

Tetrahedron, 2012 Nov; 68(44):9095-9104.

2. Win-Piazza H, Schneeberger VE, Chen L, Pernazza D, Lawrence HR, Sebti SM,

Lawrence NJ and Wu J. Enhanced anti-melanoma efficacy of interferon alfa-2b via

inhibition of Shp2. Cancer Letters, 2012 Jul; 320 (1): 81-85.

3. Scott L, Chen L, Daniel K, Brooks W, Guida W, Lawrence H, Sebti S, Lawrence N

and Wu J. Shp2 protein tyrosine phosphatase inhibitor activity of estramustine

phosphate and its triterpenoid analogs. Bioorg. Med. Chem. Lett. 2011; 21: 730-733.

4. Ren Y, Chen Z, Chen L, Fang B, Win-Piazza H, Haura E, Koomen JM, Wu J.

Critical Role of Shp2 in Tumor Growth Involving Regulation of c-Myc. Genes &

Cancer. 2010 Oct;1(10):994-1007.

5. Philpott N, Bakken T, Pennell C, Chen L, Wu J, and Cannon M. The KSHV G protein-coupled receptor contains an Immunoreceptor tyrosine-based inhibitory motif

that activates Shp2. Journal of Virology. 2011 Jan; 85(2): 1140-1144.

6. Chen L, Pernazz D, Scott L, Lawrence H, Ren Y, Luo Y, Wu X, Sung S, Guida W,

Sebti S, Lawrence N, and Wu J. Inhibition of cellular Shp2 activity by a methyl ester

analog of SPI-112. Biochemical Pharmacology. 2010 Sep; 80 (6): 801-810.

7. Wahle J, Paraiso K, Kendig R, Lawrence H, Chen L, Wu J, Kerr W. Inappropriate

recruitment and SHP1 Activity is responsible for receptor dominance in the SHIPdeficient NK cell. Journal of Immunology. 2007, 179: 8009-8015.

8. Ren Y, Chen Z, Chen L, Woods N, Reuther G, Cheng J, Wang H, and Wu J.

SHP2E76K mutant confers cytokine-independent survival of TF-1 myeloid cells by upregulating BCL-XL. J. Biol. Chem. 2007, 282(50):364**-*****.

9. Lawrence H, Pireddu R, Chen L, Sung S, Szymanski A, Yip R, Guida W, Sebti S,

Wu J and Lawrence N. Inhibitors of Shp2 Protein Tyrosine Phosphatase Based on

Oxindole Scaffolds. J. Med Chem. 2008 Aug; 51(16): 4948-4956. ( :co-first author)

10. Chen L, Sung L, Yip R, Lawrence H, Guida W, Sebti S, Lawrence N, and Wu J.

Discovery of a Novel Shp2 Protein Tyrosine Phosphatase Inhibitor. Mol Pharmacol.

2006 May 22; 70:562-570.

11. Chen L, Meng S, Bali P, Wang H, Bali P, Bai WL, Li BY, Atadja P, Bhalla K, Wu J.

Chemical ablation of androgen receptor in prostate cancer cells by the histone

deacetylase inhibitor LAQ824. Molecular Cancer Therapeutics. 2005; 4(9): 1311-9.

12. Chen L, Gao C, Chen J, Xu X, Zhou D, Chi Z. Internalization and recycling of

human mun opioid receptors expressed in Sf9 insect cells. Life Sciences. 2003; 73(1):

115-128.

13. Gao C, Chen L, Tao Y, Chen J, Xu X, Chi Z. Effects of ohmefentanyl stereoisomers

on phosphorylation of cAMP-response element binding protein in cultured rat hippocampal neurons. Acta Pharmacol Sin. 2003 Dec; 24(12): 1253-8.

14. Gao C, Chen L, Tao Y, Chen J, Xu X, Zhang G, Chi Z. Colocalization of phosphorylated CREB with calcium/calmodulin-dependent protein kinase IV in hippocampal neurons induced by ohmefentanyl stereoisomers. Brain Research. 2004;

1024(1-2): 25-33.

15. Gao C, Chen L, Chen J, Xu X, Chi Z. Ohmefentanyl stereoisomers induce changes of CREB phosphorylation in hippocampus of mice in conditioned place preference

paradigm. Cell Res. 2003; 13(1): 29-34.

16. Chen L, Gao C, Zhou D, Wei Q, Xu X, Chen J, Chi Z. Homomerization of human

mu-opioid receptor overexpressed in Sf9 insect cells. Protein Pept Lett. 2002; 9(2):

145-152.

17. Chen L, Feng Y, Zhou D, Wei Q, Xu X, Chen J, Chi Z. One-step affinity purification

of human mu-opioid receptor overexpressed in baculovirus system. Protein Pept Lett.

2001; 8(4): 265-272.

18. Feng Y, Chen L, Zhou D, Chen J, Xu X, Chi Z. Analysis of binding domain and

function of chimeric opioid receptors to ohmefentanyl stereoisomers. Acta

Pharmacol Sin. 2001; 22(11): 981-985.

19. Wei Q, Zhou D, Shen Q, Chen J, Chen L, Wang T, Pei G, Chi Z. Human mu-opioid

receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o

proteins. Cell Res. 2000; 10(2): 93-102.

20. Chen L, Guo X, Liu W, Guo Z. Effects of lysophosphatidylcholine and endothelium

on rabbit aortic rings contractions by serotonin. Chinese J. Pharmacol and Toxicol.

1999; 13(2): 85-88.

21. Guo X, Chen L, Liu W, Guo Z. High glucose inhibits expression of inducible and

constitutive nitric oxide synthase in bovine aortic endothelial cells. Acta Pharmacol

Sin. 2000; 21(4): 325-328.

22. Guo X, Liu W, Chen L, Guo Z. High glucose impairs endothelium-dependent

relaxation in rabbit aorta. Acta Pharmacol Sin. 2000; 21(2): 169-173.

23. Guo X, Liu W, Chen L, Guo Z. High glucose enhances H2O2-induced apoptosis in

bovine aortic endothelial cells. Acta Pharmacol Sin. 2000; 21(1): 41-45.

24. Yi FX, Liu W, Chen L, Zeng S, Guo Z. Hydrolysis of extracellular adenine

nucleotides by cultured bovine endocardial endothelial cells. Acta Pharmcol Sin.

1999; 20(8): 745-749.

25. Li Z, Chen L, Guo Z. Pharmacokinetics and relative bioavailability of a suspension

formulation of ibuprofen in normal volunteers. Hunan Medical Journal. 1999; 16(4):

243-244.

REFERENCES

Celso P. Silva, M.D.

Center for Reproductive Medicine

3435 Pinehurst Avenue Orlando, FL 32804-4049

410 Celebration Place Suite 205 Celebration, FL 34747

Tel: 407-***-**** Fax: 404-***-****

Email: *****.*****@**********.***

Dahui Qin, M.D. and Ph.D.

Medical Director of Molecular Diagnostic Lab

Moffitt Cancer Center

12902 Magnolia Drive. Tampa, FL 33612

Tel: 813-***-**** Fax: 813-***-****

Email: *****.***@*******.***

Jie Wu, Ph.D.

Associate Professor

Molecular Oncology Program

H.Lee Moffitt Cancer Center & Research Institutes

12902 Magnolia Dr.

Tampa, FL 33612

Tel: 813-***-****

Email: **@*******.***.***

M.L. Richard Yip, Ph.D.

Director, HTS Core

Associate Research Professor, Department of Molecular Medicine

City of Hope

1500 E Duarte Rd

Duarte CA 91010

Tel: 626-***-**** x65057

Fax: 626-***-****

Email: ****@***.***

Nicholas J. Lawrence, PhD

Associate Professor, Drug Discovery Program

H Lee Moffitt Cancer Center & Research Institute

12902 Magnolia Drive, MRC 4West

Tampa, FL 33612

Email: ********.********@*******.***

Contact this candidate