Ajinkya Pattekar

267-***-**** acwwyn@r.postjobfree.com

• Clinical Development • Pre-Clinical Research • Vaccines •SOP writing • Animal handling • Laboratory operations • Clinical and Medical experience • GLP and GCP • Immunology

PROFILE

Professional with 2 years of clinical/patient care experience and 1 year of research experience in Immunology. Diversified skill sets include interacting with patients, physical assessment and diagnosis, and providing appropriate treatment, developing and implementing research protocols, data collection and analysis, and interpreting and adhering to appropriate clinical and research practice. Doctor of Medicine, from Smolensk State Medical Academy, Russia and Master of Science in Immunology, from Drexel University, Philadelphia. Actively seeking a challenging and a growth oriented career in basic and translational research where I can utilize my combined training in clinical medicine and research in translational immunology in a team environment to contribute towards personal and organizational goals. Experience in lab animal research including working in BSL2 environment, laboratory safety and environmental hazard management, writing pre-clinical trial protocol, and data analysis using Prism graph pad and SPSS. Developed, monitored and optimized immuno-assays for pre-clinical trials.

PROFESSIONAL EXPERIENCE

Drexel University College of Medicine

May 2015 till present

Position held: Research Assistant:

Projects handled

1.Preclinical projects (Dr. Michele Kutzler, PhD, Department of Medicine)

Vaccines for Clostridium Difficile:

In-vivo testing of efficacy for DNA plasmid encoding the receptor binding domain of toxins A and B

Conducted immunization in mice, drawing blood by submandibular/heart puncture

Performed necropsy, processing of the spleen and lymph nodes

Conducted and monitored anti-toxin ELISAs using sera and mucosal specimens

Vaccines for HIV:

Development of mucosal targeting molecular adjuvants to be co-delivered with HIV-1 DNA-based vaccine antigens

Assist with immunization process followed by in-vivo electroporation in mice, drawing blood (submandibular/heart puncture), processing of the spleen and lymph nodes, and running anti-env ELISAs using sera and mucosal specimens.

Assay Development:

Development of neutralization assay to test the neutralizing function of the antibodies in the serum of immunized mice for Clostridium difficile.

Cell culture with vero cells, observing cell rounding under the microscope and caspase assay formed the basis of this assay development.

Observational training of neutralization assay and anaerobic chamber operation at Pfizer (Pearl River).

2.Clinical projects (Clinical Research Unit, Division of Infectious Diseases & HIV Medicine)

Clinical research studies of HIV-1 infected patients at the Partnership Comprehensive Care Practice

Studied immune response to HIV and other infections, and the potential for immune-based therapies to reverse the dysfunction

Written SOP’s for processing and long term storage of patient blood specimens, isolation of PBMCs, plasma and sera from blood tubes, cryopreservation of cells, maintaining log sheets for specimen storage, and shipment of patient specimens to study sites

Examined the relationship between physical function, co-morbidities, and inflammation in women with HIV.

Measure and analyzed the frequency and absolute cell numbers of CD4+ and CD8+ T cells that express CD28+/HLA DR/CD38+ using flow cytometry and ELISA for detection of the inflammatory biomarkers IL6,CRP, D-dimer and sTNFR1

Run the cytokine ELISAs for inflammatory markers in the serum as well as to stain the PBMCs using tittered and optimized antibody panels during flow cytometric analysis of activated T cells

3.Clinical experience

Assisted Physicians to provide medical care in departments of Medicine, Surgery, Orthopedics, Emergency, Pediatrics, Obstetrics and Gynaecology

Duties included taking patient history, primary evaluation of the patients, prescribing medicines in an OPD, phlebotomy, performing small surgical procedures, participate in differential diagnosis discussion with the lead physician.

RESEARCH SKILLS

Clinical: Patient specimen processing for clinical research projects including sera, plasma and PBMC purification from whole blood, stool processing for mucosal antibody analysis, writing of laboratory standard operating procedures, storage and record keeping of specimens.

Molecular and cell biology: Cell culture, SDS-PAGE, western blot, ELISA, ELISPOT, cell lysate preparation, cell counting using hemacytometer and countess systems, light microscopy.

Small animal procedures: Blood collection (submandibular/heart puncture), necropsy and tissue isolation and processing into single cell suspensions (liver, spleen, popliteal LN, inguinal LN, mesenteric LN, payers patches), intraperitoneal and intramuscular injections, in vivo electroporation delivery of DNA vaccines.

EDUCATION & CREDENTIALS

1.Masters Student, Immunology

Drexel University, College of Medicine, PA USA 2014-2016

GPA: 3.74

2.Doctor of Medicine (MD) (Physician)

Smolensk State Medical Academy, Russia 2005-2011

GPA: 3.10

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