Microsoft Office Assistant

Felix Alonso-Valenteen linkedin.com/felixalonsovalenteen

***** ******** ***** ****, *********** PA 17236 717-***-**** *******@*****.***

EDUCATION

Cedars Sinai Graduate School of Biomedical & Translational Medicine June 2015

Ph.D. in Biomedical Science & Translational Medicine

Thesis: Immunostimulatory nanoparticle-delivered siRNA for Breast Cancer

St. Joseph’s University, Philadelphia PA June 2010

B.S. in Biology

RESEARCH EXPERI ENCE

Cedars Sinai Biomedical Sciences, Los Angeles, CA 2011 - Present

Graduate Researcher

Advisor: Dr. Medina-Kauwe, PhD

His-Tag protein production and purification

Quantify gene expression

Form nanoparticle complexes

Characterize nanoparticles using DLS, HPLC and cryoEM

Evaluate cellular trafficking using Fluorescent and Confocal Microscopy

Quantify and qualify cell death through proliferation assays, IHC and Microscopy

Carry out small animal in vivo tumor experiments and biodistribution through bioluminescence assays

Saint Joseph’s University Summer Scholars Program, Philadelphia, PA 2008

Undergraduate Researcher

Advisor: Dr. Snetselaar, PhD

Maintain mycological and phytological samples

Carry out mycological infection assays

Quantify response via fluorescent and confocal microscopy

Replicate physical, chemical and biological conditions via silicon replicates

TEACHING/MENTORING EXPERIENCE

Saint Joseph’s University Biology Department, Philadelphia PA Fall 2008-Spring 2010

Teaching Assistant-Organismic Biology

Organized and coordinated laboratory exercises,

Graded quizzes and papers

Cedars-Sinai Medical Center Fall 2011-Present

Medina-Kauwe Lab

Met daily with second year graduate student to review current experiments, etc.

Big Brother Graduate Student Program

Mentored incoming students through first year of classes and acclimation to the lab environment as well as teaching methodology.

PUBLICATIONS

Papers:

1.Jae Youn Hwang, David J Lubow, David Chu, Jessica Sims, Felix Alonso-Valenteen, Harry B Gray, Zeev Gross, Daniel L Farkas, Lali K Medina-Kauwe. “Photoexcitation of tumor-targeted corroles induces singlet oxygen-mediated augmentation of cytotoxicity”. Journal of Controlled Release, 2012; DOI:10.1016/j.jconrel.2012.09.015

2.Blumenfeld CM, Sadtler BF, Fernandez GE, Dara L, Nguyen C, Alonso-Valenteen F, Medina-Kauwe L, Moats RA, Lewis NS, Grubbs RH, Gray HB, Sorasaenee K.” Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate..J Inorg Biochem. 2014 Nov;140:39-44. doi: 10.1016/j.jinorgbio.2014.06.015. Epub 2014 Jun 28

3.Jessica D. Sims, Jae Youn Hwang, Shawn Wagner, Felix Alonso-Valenteen, Gevorg Karapetyan, Karn Sorasaene, Ahmed Ibrahim, Eduardo Marban, Rex Moats, Harry B. Gray, Zeev Gross, Lali K. Medina-Kauwe. “A corrole nanobiologic elicits tissue-activated MRI contrast enhancement and tumor-targeted toxicity” Journal of Controlled Release. Volume 217, 10 November 2015, Pages 92–101

Abstracts:

1.Alonso-Valenteen, F, Medina-Kauwe, L. T7-phage polymerase modification of siRNA serves to elicit immune response. Cedars-Sinai Research Day, Los Angeles, CA, 2013.

2.Alonso-Valenteen, F, Medina-Kauwe, L AACR, San Diego 2014 meeting

3.Alonso-Valenteen, F, Medina-Kauwe, L Cedars-Sinai Research Day, Los Angeles, CA, 2014.

PRESENTATIONS

RECOOP 2015-Presented dissertation research to multinational nanoparticle group.

Samuel Oschin Cancer Program 2014, Cedars-Sinai

“Moving Target” 2014 USC pharmacological Sciences Program

Graduate Student Symposium 2011-2015

PROFESSIONAL AFFILIATIONS

American Association of Cancer Research (AACR)

Women’s Cancer Program, Cedars-Sinai

American Association of Immunologist (AAI)

HONORS AND AWARDS

Presidential Scholarship Award, Saint Joseph’s University Fall 2006-Spring 2010

Pre-med Alumni Scholarship Award, Saint Joseph’s University Fall 2007-Spring 2010

BIOLOGY SKILLS

Nanoparticle Characterization: Dynamic Light Scattering (DLS), cryoEM, tEM, HPLC.

Nanoparticle Development: Nucleic Acids, metallated corroles, nano-silicon, nano-sized metals with and without protein conjugation (Silver, Platinum, titanium, Copper, cysteine-silver conjugations)

Biochemistry and protein analysis: Western blot, cell surface labeling of proteins by

biotinylation, immunoprecipitation. Production of His-tagged proteins in

E.coli. Electrophoretic Mobility Shift Assay (EMSA), GST pulldowns, Chromatin

Immunoprecipitation (ChIP) assays. In vitro kinase assay.

Molecular Biology: All standard molecular biology techniques, gene cloning,

subcloning, plasmid constructs, automated sequencing, Southern Blot, Northern Blot, PCR, RTPCR and real-time quantitative PCR, oligonucleotide design. DNA and RNA isolation from rodent tissues. RNAi, subcellular fractionation, cellular trafficking. Permebealizing and non-Permebealizing ELISA.

Cell Biology and Histology: Cell culture on established and patient derived lines, cell sorting, transfection, cell cloning. Confocal immunofluorescence,IHC, H&E, gram staining

histochemistry and cytochemistry. MTT cell proliferation assay. FRET (Fluorescence

Resonance Energy Transfer).

Immunology: Antibody labeling, flow cytometry, multicellular IHC analyses, ELISA.

Virology: Lentivirus production, transfection of mammalian and non-mammalian cell lines, multiplex cytokine analyses.

in vivo: small animal handling, anesthesia, analgesia, subdermal and orthotopic tumor implantation, I.V. and I.P. injections, non-survival surgery, necropsy, tissue processing.

COMPUTER SKILLS

Operating System: Windows, iOS, Linux

Software: Microsoft Office, Python, Photoshop, ImageJ, SPSS

Modeling Software: Madonna

LANGUAGE SKILLS

English (bilingual proficiency), Spanish (native), French (professional proficiency)

REFERENCES

Dr. Lali Medina-Kauwe, PhD

Phone: 818-***-****

Email: ****.******-*****@****.***

Dr. Jessica Sims, PhD

Phone: 617-***-****

Email: *******.****@****.***

Dr. William Audeh, MD

Phone: 310-***-****

Email: *******.*****@****.***

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