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Res. Assistant / Associate (Molecular Biology, Genomics, Neuroscience)

Location:
Boston, Massachusetts, United States
Posted:
October 20, 2016

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ABDURRAHMAN W. MUHTASEB

* ********* ****, *********, ** 02048 (323) ***-**** acw5y9@r.postjobfree.com SUMMARY

I hold an MSc. degree in biochemistry and molecular biology with experience in mammalian primary and neural stem cell culture (2D & 3D) and differentiation, molecular biology and genomics (RT-qPCR, NGS), and neuroscience research. I’m seeking a research position in which I can utilize my knowledge and skills and expand them further. EDUCATION

MSc: Biochemistry and Molecular Biology Aug. 2014 – May. 2016 Keck School of Medicine of the University of Southern California – Los Angeles, CA. BSc: Biotechnology and Genetic Engineering Sep. 2009 – Jun. 2013 Jordan University of Science and Technology – Irbid, Jordan. ACCOMPLISHMENTS

Summer research fellowship for master students in Biochemistry. Keck School of Medicine of USC (2015).

Co-founded the “Eureka” Mobile Science Lab for underprivileged school children in Jordan.

Alumnus of the study of the United States institutes for student leaders, US Department of State (2012).

Writer, science contributor and communicator on multiple platforms in Arabic and English. TECHNICAL SKILLS

Rodent handling / dissection, phlebotomy, cryosectioning, and media and reagent preparation and optimization.

Mammalian primary and neural stem cell culture and maintenance (2D & 3D), and neural stem cell differentiation.

SDS-PAGE and protein analysis: Immunostaining (ICC, IHC, western blot) and other Immunoassays (ELISA).

Nucleic acid extraction and purification from tissue, blood, monolayer and 3D cultures.

Conducting different variants of PCR, primer and plasmid design, and gel electrophoresis (Southern blot, RFLP).

Nucleic acid quantitation and analysis (qPCR & RT-qPCR, NanoDrop Fluorospectrometer, and TapeStation).

Library preparation for NGS / RNA-Seq (Illumina Hi-Seq). Statistical data analysis (DESeq2, Partek GS, MS Excel).

Light and fluorescence microscopy and cell imaging. Flow Cytometry and FACS analysis. EXPERIENCE

Graduate Research Assistant

Zilkha Neurogenetic Institute of USC – Los Angeles, CA Jan. 2015 – present Culturing and differentiating neural progenitor cells derived from the olfactory neuroepithelium in 3D as a model system for studying the genetics of schizophrenia and other neurodevelopmental conditions.

Cryosectioning, immunostaining (ICC and IHC), light and fluorescence microscopy and cell imaging.

RNA isolation from 3D cultures, amplification, quantitation, and library preparation for RNA-Seq.

Transcriptome data analysis (statistical analysis, gene ontology, differential gene expression). Research Assistant

University of Jordan, School of Medicine – Amman, Jordan Apr. 2014 – Sep. 2014 Studying the genetic basis of autism in the Jordanian population. Clinical Genetics Assistant.

Collecting blood samples (phlebotomy), culturing white blood cells for cytogenetic analysis (G-banding and FISH), and DNA extraction for sequencing and genotyping.

Undergraduate Research Assistant / Intern

Jordan University of Science and Technology – Irbid, Jordan Mar. 2011 – Dec. 2013

Rodent handling and dissection. Primary and mesenchymal stem cell culture. Medium and solution preparation.

Molecular techniques: DNA extraction, PCR, gel electrophoresis, microarray validation, Sanger sequencing / analysis.

Biochemical techniques: spectroscopy, SDS-PAGE, western blot, immunoassays (ELISA), flow cytometry.



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