Archana Sareen

acvxcp@r.postjobfree.com

**** ***** **** *, ********, MN 55455. 651-***-****

EDUCATION

Pune University, Division Biotechnology Pune, India Ph.D in infectious immunology and biotechnology January 2010 Guru Gobind Singh Indraprastha University Delhi, India Bachelor in Technology in Biotechnology August 2003 Masters in Technology in Biotechnology January 2005 RESEARCH EXPERIENCE

Guru Gobind Singh Indraprastha University 2003-2005 B.Tech and M.Tech project with Dr. Nimisha Sharma

Protein Protein Interaction of Rpb4 and Rpb7 subunits of RNA polymerase II in yeast Pune University 2005-2008

Graduate student with Dr. Bhaskar Saha

• Plasticity in a bi-modular assembly of CD40 receptor activated kinases (Infectious immuology)

• Phosphatase SHP-1 plays a crucial role in CD40 signaling reciprocity (Infectious immuology) University of Minnesota 2008-2010

Research Assistant with Dr. Reuben Harris

Post-translational modifications and interaction of APOBEC3 with its subunits by yeast two hybrid system and Mass spectrometry

University of Minnesota

Postdoctoral Associate with Dr. Alexandra Sobeck and Dr. LiNa Wei 2010-2013

• Fanconi Anemia proteins exhibit different DNA damage responses during S-phase

• FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery

• RIP140 expression in T cells and its different subtypes University of Minnesota

Research Associate with Dr. Ashok K. Saluja 2014-2016

• Preclinical trial of Sun Biopharma company developed drug SBP-101 for pancreatic cancer

• Studying the effects of phase II clinical trial drug Minnelide in pancreatitis

• Release of cathepsin-B in cytosol causes cell death in acute pancreatitis

• Morphine analgesia Worsens Acute and Chronic Pancreatitis in Mice University of Minnesota

Researcher5, Lab Manager and Research Safety officer 2016-present

• Pain management for pancreatitis patients

• Preclinical trial of Antidote for cyanide toxicity in rabbits

• Write grants and budgets for projects.

• Supervise and mentor junior personnel, collaborate with other labs for different projects SKILLS AND TECHNIQUES

1. Develop, standardize and characterize reagents, test protocols, troubleshoot problems. 2. Molecular Biology:

• Cloning, DNA/RNA isolation, Protein expression and Purification, Yeast two hybrid, Characterize protein-protein interactions, q- PCR, lentivirus transduction in primary cells and cell-line, siRNA and overexpression transfection in cell-line, site-directed mutagenesis, phospho-mutant cloning, qRT-PCR. Experience working in BSL2 laboratory environment. 3. Protein Biochemistry:

• Immuno-precipitation, Co-immunoprecipitation, Western blots, immunoassays, biochemical analysis of enzymes with kinase and phosphatase assays for measurement of their activity, Dot-blot 4. Writing laboratory protocols, SOPs and supporting documents for all the protocols and good laboratory practice:

• T-cell proliferation assays, Multi-color flow cytometry, Antibody ELISA, Elispot, Lymophocyte isolation from immune organs and tumors. Experience in mouse tumor models. 5. Computer knowledge:

• MS work pad, excel, power point, adope photoshop, imageJ, statistical analysis. 6. Knowledge of GLP, murine model development.

COMMUNICATION EXPERIENCE

• Invited Talks: Department of Surgery, Pharmacology and Biochemistry, University of Minnesota.

• Reviewer for 4 journals: Molecular and Cellular Biochemistry, Journal of Pharmacology and Experimental Therapeutics, American Journal of Chinese Medicine

• Editorial board for 2 journal. International Journal of Clinical Immunology (Reviewer and editorial board). Science Publishing group (Editorial Board).

• Collaborated with Sun Biopharma company to work in a team of 50 people on preclinical trial drug SBP- 101 using immumoassays and apoptosis assays. Presented my work in a conference consisted of 500 people. http://globenewswire.com/news-release/2015/11/09/785142/10155580/en/Compelling- Preclinical-Data-on-Sun-BioPharma-s-SBP-101-Presented-at-the-2015-American-Pancreatic- Association-APA-Annual-Meeting.html

PEER-REVIEWED PUBLICATIONS

1. Sareen A, Choudhry P, Mehta S, Sharma N. Mapping the interaction site of Rpb4 and Rpb7 subunits of RNA polymerase II in Saccharomyces cerevisiae. Biochem Biophys Res Commun, 2005. 332(3): p. 763-70.

2. Sarma U, Sareen A, Maiti M, Kamat V, Sudan R, Pahari S, Srivastava N, Roy S, Sinha S, Ghosh I, Chande AG, Mukhopadhyay R, Saha B. Modeling and experimental analyses reveals signaling plasticity in a bi-modular assembly of CD40 receptor activated kinases. PLoS One. 2012;7(7):e39898. Epub 2012 Jul 18.

3. Sareen A, Chaudhury I, Adams N, Sobeck A. Fanconi Anemia proteins FANCD2 and FANCI exhibit different DNA damage responses during S-phase. Nucleic Acids Res. 2012 Sep 1;40(17):8425-39. 4. Chaudhury I, Sareen A, M Raghunandan, Sobeck A. FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery. Nucleic Acids Res. 2013 Jul;41(13):6444- 59.

5. Khan TS, Srivastava N, Srivastava A, Sareen A, Mathur RK, Chande AG, Kumari S, Musti KV, Roy S, Mukhopadhayay R, Saha B. SHP-1 plays a crucial role in CD40 signaling reciprocity. J of immunology 2014 Oct 1;193(7):3644-53.

6. Release of cathepsin-B in cytosol causes cell death in acute pancreatitis. Rupjyoti Talukdar*, Archana Sareen*, Hongyan Zhu, Vikas Dudeja, Usman Barlass, Raghuwansh Sah, Sushil K Garg, Sulagna Banerjee, Pramod Garg, Rajinder Dawra, Ashok K Saluja (Revised and submitted to Gastroenterology).

7. Comprehensive analysis of microRNA signature of mouse pancreatic acini identifies over- expression of miR-21-3p in acute pancreatitis. Ajay Dixit, Anne Sarver, Zuobiao Yuan, John George, Usman Barlass, Hassam Cheema, Archana Sareen, Sulagna Banerjee, Vikas Dudeja, Rajinder Dawra, Subbaya Subramanian, and Ashok Saluja. (Submitted to American Journal of Physiology- Gastrointestinal and Liver Physiology)

PUBLISHED CONFERENCE PAPERS

1. A Dixit, R Dawra, U Barlass, A Sareen, Z Yuan, A Sarver, S Subramanian, A Saluja. Tu1887 microRNAs Profiles of Mouse Acinar cells and its implications in Acute Pancreatitis. Gastroenterology 148 (4), S-928.

2. A Sareen, R Dawra, AK Saluja. Triptolide inhibits NF-κB mediated pro-inflammatory cytokines production in macrophage and acinar cells. Pancreas 43 (8), 1404 3. Integrin-linked Kinase Pathway Plays an Important role in Initiating Acute Pancreatitis. Z. Yuan, J. George, C. Ulrich, U. Barlass, S. G. Kumar, A. Sareen, A. K. Dixit, R. K. Dawra, A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota Medical School, Minneapolis, MN, USA. (Poster of distinction award). 4. Chemically Direct Inhibition of Nuclear Factor κB only Ameliorates Local Pancreatic but not Remote Lung Inflammation via Up-regulating Pancreatitis Associated Protein 1. C. Ulrich*, Z. Yuan*, J. George, U. Barlass, S. G. Kumar, A. Sareen, A. K. Dixit, R. K. Dawra, A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota Medical School, Minneapolis, MN, USA. (Poster of distinction Award). *Equal contribution. 5. Morphine analgesia Worsens Chronic Pancreatitis in Mice J. George U. Barlass, A. Dixit, Z. Yuan, A. Sareen, S.K Garg V. Dudeja, S. Roy, R. Dawra, A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis MN 6. Evaluation of Minnelide on granulocyte differentiation and apoptosis. V. K. Gupta, A. Sareen, S. Modi, B. Giri, S. Banerjee and A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis MN. 7. SBP-101 Induces Apoptosis in Mouse Pancreatic Acinar Cells and Inhibits Polyamines Induced Proliferation. A. Sareen1, A.Saluja1, A. Shah2, R. Dawra1 1Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis MN 2Sun BioPharma, Inc., Williston, FL.

8. Extracellular ATP causes pancreatic necrosis leading to systemic inflammation. A. Dixit, Barlass, Z. Yuan, J. George, A. Sareen, S.K Garg V. Dudeja, S. Roy, R. Dawra, A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis MN 9. Role of miR-21-3p in Promoting Inflammation During Acute Pancreatitis. A. K. Dixit, J. George, U. Barlass, Z. Yuan, A. Sareen, V. Dudeja, R. Dawra, S. Subramanian, A. K. Saluja. Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis MN.

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