Negar Behnamfar
*****.*********@*****.***
San Diego, CA, 92108
Objective
To establish a career that allows me to use my current skills and knowledge and expand them to include new topic. Education
Shiraz University of Medical Science, Iran
Master of Science in Immunology
(2011-2014)
Isfahan University, Iran
Bachelor of Science in Microbiology
(2006-2010)
Skills and Qualifications
• Excellent skills in Western Blot, Immunostaining
• Proficiency in Flowcytometry
• Strong skills in cellular biology with sterile techniques: maintenance of cell culture assays
• Frequently operate laboratory machines and laboratory computer software/programs:
• Microsoft Office, Photoshop, Image Studio Lite, Flowjo, GraphPad Prism
• Excellent communication and ability to work with people Laboratory Experience
The Laboratory of Dr. Dwayne Stupack at Moores Cancar Center, San Diego, CA ( Jun 2015- Now)
• Performed a variety of experiments that support research activities in various disciplines, includes operating scientific equipment, selecting appropriate methods and techniques to perform experiments, analyzing data and preparing related reports.
• Experienced fluorescence and bright field microscopy and image analysis.
• Provided testing using molecular biology techniques: Western blot, Immunofluorescence
• Assisted in the creation of new standard operating procedures and validation of new assays/equipment/supplies.
• Maintained quality control checks and a safe environment in compliance with all government and university policies, rules and regulations
• Skilled in Cell Culture in high quality lab work. The Laboratory of Dr. Doroudchi at T cell Laboratory, Shiraz, Iran ( 2012-2014)
• Performed Isolation of PBMC, Freeze and Thaw
• Experienced in Intracellular Staining and Extracellular Staining
• Skilled in Flowcytometry
Microbiology Experience
• Performed making Culture Medium
• Experience in doing microbial tests
Publications
Behnamfar N, Zibaeenezhad MJ, Golmoghaddam, Doroudchi M. CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis. Cell Mol Biol. 2015 Dec 9;61(8):17-23. Keller N, N. Behnamfar, Chen L, Quach W, Graf R, Moresco J, Yates J, Stupack D. Distinct mechanisms of caspase-8 modulation by Src family tyrosine kinases. manuscript under review. Nadine Keller, Lauren E. Chen, Ryon P. Graf, Negar Behnamfar, Xuemei Huang, Stanley J. Opella, Dwayne G. Stupack. Regulation of Phosphoinositide 3-Kinase (PI3K) Activity by Caspase-8 Binding to the N-terminal Src- Homology 2 (nSH2) Domain of p85α. manuscript under review. Ryon P. Graf, Nadine Keller, Alena Bartakova, Katelyn E. McCabe, Isabelle Tancioni, Vincent J., Joe R. Delaney, Mina Haghighi, William T. Quach, Negar Behnamfar, David D. Schlaepfer, Dwayne G. Stupack. Caspase-8 as a Prometastatic Factor in Breast Cancer. manuscript under review. Presentation / Poster
Th17 deviation of peripheral blood memory CD4+ cells in Atherosclerosis (It was chosen as excellent poster presentation in 12th International Congress of Immunology & Allergy in Tehran, 2014)
T cells and Co-stimulation in Atherosclerosis (M.Sc. seminar 2012) Activities and Organizations
Recognized for outstanding support in Lupus Foundation of American’s Walk to end Lupus (2015 & 2016) Reference
Available upon request