Quality Control Data

David A. Stiles

*** *** ******, ***. ***

Gaithersburg, MD 20877

Cellphone#: 240-***-****

Email: *********@*****.***

EDUCATION

M.A., Cell Biology

Marshall University, Huntington, WV

2001

B.S., Biological Sciences

Marshall University, Huntington, WV

1999

EMPLOYMENT HISTORY

Employer Position Title Dates

National Institutes of Health Clinical Center (CC)

Clinical Research Specialist

01/2012 – Present

Gene Logic An Ocimum Biosolutions Company

Quality Control and Data Analyst

08/2008 – 01/2012

Team Placement Services Inc. Contractor at the National Eye Institute (NEI)

Research Associate

04/2007 – 08/2008

National Institutes of Diabetes, Digestive, and Kidney Diseases (NIDDK)

Technologist/Research Assistant

01/2003 – 02/2007

SUMMARY OF EXPERIENCE

While working at Gene Logic and the National Institutes of Health (NIH) from 2003 to 2012 Mr. Stiles developed computational skills for qRT-PCR, the Affymetrix GeneChipTM and commercial dual labeled oligo microarray data analysis. These skills include extensive experience with parametric and nonparametric statistical analysis methodologies for the analysis of microarray and quantitative real-time PCR (qRT-PCR) data (e.g., t-test, ANOVA, PCA, and clustering). He is an expert in the use of Microsoft Office, GeneSpring GX, Partek Genomics Suite, and R software packages for analysis of microarray gene expression datasets. Mr. Stiles has a theoretical and working knowledge of the comparative Ct and standard curve methods to quantifying differential gene expression.

Mr. Stiles has assay design experience using Taqman and SYBER Green based qRT-PCR. His design experience includes the Taqman MGB assay for the detection of short genomic DNA insertions, deletions, and substitutions; as well as Long Range (LR) and Short Range (SR) PCR assays for the Affymetrix resequencing technology platform and Sanger Based Sequencing methods.

For bioinformatics Mr. Stiles has the following skills: computer programming experience with Matlab, SAS Enterprise Guide, and R. Additionally he has a well-rounded working knowledge of NCBI (ex. BLAST) and UCSC bioinformatics resources (ex. BLAT and Genome Viewer) and other web based bioinformatic tools.

EXPERIENCE

National Institutes of Health Clinical Center, Bethesda, MD

Clinical Research Specialist

Investigates research objectives relevant to the overall goals of the laboratory. Scope and objectives are discussed and defined by the Chief, Laboratory Services Section (LSS). Performs literature review pertinent to the project to identify the most applicable protocols and methods. Develops research objectives based on working models as a result of work experience, scientific knowledge, and review of pertinent literature.

Assumes major responsibility for planning and development of experiments and protocols. Recommends appropriate methods, and develops and modifies methods and procedures as appropriate. Recognizes problems or deficiencies in methodologies or protocols and takes appropriate corrective actions. This may involve the evaluation of new or unfamiliar methods. Determines time and resources required to perform experiments and participates in the establishment of plans and schedules. Selects and obtains the appropriate amounts and kinds of reagents, media, and samples required. Consults with other co-workers in obtaining reagents and equipment. Assures that instrumentation to be used is in good working order and is properly maintained and calibrated. Establishes working relationships with researchers in other laboratories within DTM and outside DTM, when appropriate, to obtain access to other equipment if necessary. Performs experiments in accordance with established protocols. Critically analyzes data to ensure it is accurate and meets to the goals of the experiments. Discusses the results of experiments with the Chief, LSS and may give informal presentations at staff meetings when appropriate. Analyzes data, applying standard statistical methods such as dispersion, analysis of variance, and regression and correlation analysis. Assembles data in tabular (tables, graphs, charts) and narrative form, noting inconsistencies or variances. Interprets findings and summarizes conclusions.

Gene Logic, Gaithersburg, MD

Quality Control and Data Analyst

Provided qRT-PCR and microarray data analysis services to Gene Logic clients and for internal research and development projects using appropriate data analysis software packages. Develops R scripts to automate the quality control of Agilent, Affymetrix, and Illumina Microarray data using manufacturer’s recommended metric thresholds. Helps develops standard operating procedures for the quality control of qRT-PCR and microarray data generated at Gene Logic. Performs routine quality control data analysis of in-house generated qRT-PCR and microarray data for Gene Logic clients and internal research and development projects.

National Eye Institute (NEI), Bethesda, MD

Research Associate (US Government Contractor with Team Placement Service Inc.)

Provided assistance to one or more senior investigators by performing laboratory experiments in support of biomedical research programs and projects. Was responsible for exercising independent judgment in performing a full range of responsibilities in support of the ongoing research of the laboratory. Responsible for selecting, devising, planning and executing the most appropriate experiments, for analysis and interpretation of the data, for developing strategies for pursuing significant observations and for insuring the accuracy and reproducibility of determinations. Served as an expert resource in molecular biology, and cell biology techniques. Trained personnel, research associates, post-doctoral fellows, visiting scientists, students and other assigned personnel in specialized laboratory techniques, procedures and laboratory safety, especially with regard to radioactive, biohazardous or other hazardous materials. Managed computer software and hardware needs and recommends equipment and supplies for the laboratory for procurement. Developed and performs a variety of complex experimental techniques and procedures in support of the laboratory investigations pursued by Unit personnel and scientists from other Institutes or institutions. Was responsible for determining experimental approaches to be applied in order to evaluate the hypotheses under investigation and for planning and carrying out those experiments. Performed and interpreted a variety of complex experimental techniques and procedures; searches all pertinent literature and references; devises, modifies and performs the analyses; evaluates and interprets the data; makes modifications as needed; communicates results and methods in reports, presentations, and manuscripts for peer-reviewed publications. Provided technical advice to others within NEI and other federal or academic research environments on all matters in the specialty area. Upon completion of experiments, actively participates and collaborates in the preparation of scientific publications based on the results; documents information on procedures, modifications and methods for input info final publication and presentations of findings at local, national and international meetings.

National Institute of Diabetes, Digestive, and Kidney Diseases (NIDDK), Bethesda, MD

Technologist/Research Assistant

Participate in the design of research studies by selecting the appropriate methodology and procedure to meet experimental objectives. Performed microarray experiments including preparation of RNA and quality control of RNA from tissues or cells, synthesis of fluorescently labeled target cRNA or cDNA, array hybridization, and optical scanning of arrays. Calibrated and operated a variety of laboratory instruments used to perform tests, analysis and other laboratory procedures. Assisted in the analysis and interpretation of data and prepared summaries of laboratory findings to be used in reports and papers to present at scientific meetings and/or publication in scientific journals. Provided training to students and other laboratory personnel in the use of techniques, procedures, and equipment to complete objectives. Managed quality control of the laboratory including upkeep and routine maintenance of equipment. Performed computer analysis of data and used computer software to prepare data for publication.

CONTINUING EDUCATIONAL COURSES AND TRAINING

Introduction to Object Oriented Programming using Java (John Hopkins University, 3 Credit Hours)

Fundamentals of UNIX (NIH/Center for Information Technology)

MATLAB Fundamentals and Programming Techniques (The MathWorks Training Services)

Statistical Methods in MATLAB (The MathWorks Training Services)

Basic Perl Programming (NIH/Center for Information Technology)

Field Guide to NCBI Molecular Biology Resources (NIH/NLM/NCBI)

Introduction to Biostatistics STAT 200 (6 Credit Hours)

Programming with NCBI BLAST (NIH/NLM/NCBI)

Beckman Coulter Biomek FX Fundamentals, 3.2 Software Course (Beckman Coulter)

Beckman Coulter Biomek FX Methods Programming, 3.2 Software Course (Beckman Coulter)

Onsite Training and application development with Freedom Evo Liquid Handling Workstation (Tecan)

PRESENTATIONS AND ABSTRACTS

“eyeArray for Detection of Mutations in Common Genes Involved With Inherited Retinal Dystrophies” MacDonald IM, Smaoui N, Stiles D, Ben Hamad M, Strunnikova N, Hejtmancik JF. Association for Research in Vision and Ophthalmology Poster Presentation (2008).

“Cover Art for the AABB Journal Transfusion”

David Stiles, Willy A. Flegel

Helped designed the cover art for the AABB journal Transfusion, December 2014, Volume 54, Issue 12.

“Two Large Intergenic Deletions in RHD Involving Either Exon 1 or Exon 10”

David Stiles, Kshitij Srivastava, Franz Wagner, Willy Flegel. AABB Annual Meeting (2015)

PUBLICATIONS

“Long CGG-repeat tracts are toxic to human cells: Implications for carriers of Fragile X permutation alleles”. Vaishali Handaa, Deena Goldwater, David Stiles, Margaret Cam, George Poy, Daman Kumaria, and Karen Usdina, FEBS Letters 579 (200*-****-****.

“A detailed transcript-level probe annotation reveals alternative splicing based microarray platform differences”. Joseph C Lee, David Stiles, Jun Lu, and Margaret C Cam, BMC Genomics 2007, 8:284.

“Disruption of S1P degradation leads to altered lipid metabolism and massive inflammation”, Meryem Bektas, David Stiles, and Richard L. Proia, Unpublished Research (2007).

“The functional effect of pathogenic mutations in Rab escort protein 1”, Y.V. Sergeev, N. Smaoui, R. Sui, D. Stiles, N. Gordiyenko, N. Strunnikova, I.M. MacDonald, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Volume 665, Issues 1-2, 1 June 2009, Pages 44-50.

“Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant”. Manas K. Chattopadhyay, Weiping Chen, George Poy, Margaret Cam, David Stiles and Herbert Tabor. Yeast, Volume 26 Issue 10, 17 Aug 2009, Pages 531 - 544.

“High-Throughput Retina-Array for Screening 93 Genes Involved in Inherited Retinal Dystrophy”.

David Stiles, Jin Song, Nizar Smaoui, Radha Ayyagari, Sonia Benhamed, Ian M. MacDonald, Stephen P. Daiger, Santa J. Tumminia, Fielding Hejtmancik, Xinjing Wang. Investigative Ophthalmology & Visual Science November 2011.

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