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Professional Experience Manager

Location:
Pasadena, CA
Posted:
April 19, 2016

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Resume:

Shuxia Meng

*

Shuxia Meng Green card

California Institute of Technology acue69@r.postjobfree.com

**** * ********** ****, *****-96 Lab phone: 626-***-**** Pasadena, CA 91125 Cell phone: 626-***-****

SUMMARY OF QUALIFICATIONS

Fifteen years' professional experience in molecular biology, biochemistry and mouse work Experience of managing lab: Ordering supplies, lab organization, equipment maintenance EDUCATION

1999-2002 Yangzhou University, China M.S. with Preventive Veterinary 1995-1999 Henan Agricultural University, China B.S. with Veterinary PROFESSIONAL EXPERIENCE

2011-Present Lab manager/Research technician, David Chan laboratory, California Institute of Technology, Pasadena, CA, USA

2004-2010 Research technician, Xiaozhong (Alec) Wang laboratory, Northwestern University Evanston, IL, USA

TECHNICAL EXPERTISE

Tools for molecular biology work:

Gene construction (PCR, electrophoresis, DNA/RNA purification, digestion, ligation, transformation, preparation for chemically/electro competent cells) Gene expression analysis (southern blot, real-time PCR, in situ hybridization, dual luciferase assay) Tools for cell culture work:

Mouse embryonic stem cell, mouse embryonic, fibroblast cell, mouse feeder cell, HEK293T cell, Hela cell, Cos7 cell, DF1 cell, and hybridoma cell Preparation of lentivirus and retrovirus

Tools for biochemistry:

Protein expression in bacterial, pichia, mammalian cultures Protein purification (gel filtration, dialysis, ion exchange) Shuxia Meng

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Crystallization

Protein detection (SDS-PAGE, western blot, ELISA assay, immunohistology) Identifying and confirming protein-protein interaction (GST pull-down, co-immunoprecipitation and yeast hybridization)

Preparation of monoclonal antibody in hybridoma cells and polyclonal antibody in rabbits Tools for mouse work:

PCR genotyping, mouse mating, glucose tolerance test Mouse whole body composition analysis by Echo Magnetic Resonance Imaging (EchoMRI) Dissecting mouse tissues; making cryostat section, paraffin section and vibratome section Tools for biological imaging:

Dissection microscopy, fluorescent microscopy, confocal microscopy Computer skills:

Blast genome sequence, Word, Excel, Powerpoint

PUBLICATIONS

Losón OC, Meng S, Ngo H, Liu R, Kaiser JT, Chan DC. Crystal structure and functional analysis of MiD49, a receptor for the mitochondrial fission protein Drp1.Protein Sci. 2015 Mar;24(3):386-94 Losón OC, Liu R, Rome ME, Meng S, Kaiser JT, Shan SO, Chan DC. The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor. Structure. 2014 Mar 4;22(3):367-77 Pham AH, Meng S, Chu QN, Chan DC. Loss of Mfn2 results in progressive, retrograde degeneration of dopaminergic neurons in the nigrostriatal circuit. Hum Mol Genet. 2012 Nov 15;21(22):4817-26 Su H, Meng S, Lu Y, Trombly MI, Chen J, Lin C, Turk A, Wang X. Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing. Mol Cell. 2011 Jul 8;43(1):97-109. Lin C, Meng S, Zhu T, Wang X. PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival. J Biol Chem. 2010 Dec 31;285(53):41675-85. Su H, Marcheva B, Meng S, Liang FA, Kohsaka A, Kobayashi Y, Xu AW, Bass J, Wang X. Gamma-protocadherins regulate the functional integrity of hypothalamic feeding circuitry in mice. Dev Biol. 2010 Mar 1;339(1):38-50.

Han MH, Lin C, Meng S, Wang X. Proteomics analysis reveals overlapping functions of clustered protocadherins. Mol Cell Proteomics. 2010 Jan;9(1):71-83. Chen J, Lu Y, Meng S, Han MH, Lin C, Wang X.Wang. alpha- and gamma-Protocadherins negatively regulate PYK2. J Biol Chem. 2009 Jan 30;284(5):2880-90 Shuxia Meng

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Meng S, Jiao X, Shao G. Preparation and Identification of Monoclonal Antibodies against the Common Antigen of Porcine Mycoplasmas. Chinese Journal of Preventive Veterinary Medicine 2003; 2 (25): 128-131

Meng S, Gao YM, Gan J. Detection of Mycoplasma gallisepticum and M. synoviae by Polymerase Chain Reaction. Chinese Journal of Veterinary Science and Technology 2002; 6 (32): 15-17 REFERENCES

Dr. David Chan (Principal investigator), Professor California Institute of Technology

Division of Biology and Biological Engineering

1200 East California Blvd, Pasadena, CA 91125

Phone: 626-***-****

Email: acue69@r.postjobfree.com

Dr. Xiaozhong Wang (Principal investigator), Associate professor Northwestern University

Department of Molecular Biosciences

2205 Tech Drive, Hogan 2-100, Evanston, IL 60208

Phone: 847-***-****

Email: acue69@r.postjobfree.com

Dr. Andrew T. Dudley, Associate Professor

The University of Nebraska Medical Center

Dept. of Genetics, Cell Biology and Anatomy

Omaha, NE 68198-5965

402-***-****

Email: acue69@r.postjobfree.com



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