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Assistant Microsoft Office

Bethesda, MD
April 12, 2016

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Lance DaSilva

***** ********** ******* ************, ** 20877


Biology and biochemistry researcher with 17 years of experience in disease states, with a focus on cancer and neurodegenerative disorders

Exceptional process and assay development skills as shown by identifying and implementing improvements to conduct complex laboratory experiments and troubleshoot SOPs and instruments

Over 10 years of experience in small and large scale protein purification (1-50L), scale-up, technology transfer

Exceptional knowledge of bioprocessing techniques, such as chromatography, filtration, sterile technique and protein analysis

Excellent customer-facing experience as evidenced by scientific collaborations, liaising with team members, and mentoring students in the laboratory, participation in lab meetings

Strong ability to review, interpret and summarize scientific and technical information in a clear and concise manner to mixed audiences as shown by presentations, research proposals and theses EDUCATION

Ph.D. University of Western Ontario, Department of Biochemistry 2008-2013 London, Ontario, Canada

Performed hands-on lab experiments to support scale-up and technology transfer for scientific research

Identified and implemented process developments for large scale protein purification to improve yield, stability, minimize degradation in order to reconstitute active DNA unwinding complexes in vitro

Showed robust DNA unwinding activity from a complex comprised of six separately purified proteins M.Sc. University of Waterloo, Department of Biology 2006-2008 Waterloo, Ontario, Canada

Performed hands-on lab experiments to support process and assay development

Identified and implemented process improvements to characterize role of Orc6 in initiation of DNA replication in budding yeast

Showed that Orc6 required to maintain MCM helicase on chromatin B.Sc. Carleton University, Honors Biology 1993-1998 Ottawa, Ontario, Canada


Post-doctoral research fellow, National Cancer Institute, Radiation Oncology Branch 2014-present Bethesda, Maryland, USA

Developing strategies to investigate the radiosensitivity effect of small molecule inhibitors on tumor survival and progression in order to identify anti-cancer therapies

Perform and troubleshoot experiments as necessary, propose future research directions

Active member of collaborative environment, participating in weekly lab meetings, exchanging ideas, providing and receiving feedback and constructive criticism

Research Assistant, Department of Biology, University of Waterloo 2003-2005 Waterloo, Ontario, Canada

Designed and conducted research on the initiation of DNA replication; identified a key factor in maintenance of DNA unwinding complex

Trained undergraduate and graduate students

Managed day-to-day lab activities, purchased and maintained lab reagents and supplies Research Assistant, Neuroscience, Ottawa Hospital Research Institute 1998-2003 Ottawa, Ontario, Canada

Examined synaptic vesicle trafficking and its role in neurodegenerative diseases; identified varied roles of protein isoforms in cellular transport

Managed day-to-day lab activities, purchased and maintained lab reagents and supplies

Trained undergraduate and graduate students



Biochemistry and Molecular Biology 2280A 2012

University of Western Ontario, London, Ontario, Canada

Teaching assistant for 40 undergraduate students, graded and provided feedback on student assignments Society of Graduate Students Council Member and Representative for the 2009-2012 Department of Biochemistry, University of Western Ontario

Represented department, attended council meetings, voiced graduate students’ concerns Clinical Biochemistry 3386B, University of Western Ontario 2009-2011 London, Ontario, Canada

Teaching assistant for 225 undergraduate students, attended lectures and provided guidance to students after class and during office hours

International Genetically Engineered Machine (iGEM) 2006-2007 University of Waterloo

Advisor for math, science and engineering undergraduate students to facilitate education and advancement of synthetic biology

Competed in iGEM jamboree in front of global audience at MIT in Boston, MA, USA RELEVANT TECHNICAL EXPERIENCE/SKILLS

Biochemistry: small and large-scale protein purification, column chromatography, AKTA, filtration, protein characterization and analyses, protein quantification and activity assays, cell-based functional assays, mass spectrometry, SDS-PAGE, Western blot, protein-protein interactions, protein-DNA interactions, radioactive work

Molecular biology: DNA sequencing, nucleic acid extraction/quantitation, genotyping, spectrophotometry, RT- PCR, qPCR, RNAi, microarray analysis, RNAseq, gene cloning, ChIP

Cell biology: cell culture, BSL-2, aseptic technique, ELISA, transformation, Flow cytometry, transfection, creation of stable cell lines, light, fluorescent and confocal microscopy

Computer skills: Microsoft Office, Unicorn, Adobe Acrobat, Photoshop, Illustrator, Partek, Ingenuity Pathway Analysis (IPA), GraphPad Prism, SigmaPlot, survival curves, gene and protein databases RESEARCH PUBLICATIONS (7 in total)

1. DaSilva LF, Pillon S, Genereaux J, Davey MJ, Gloor GB, Karagiannis J and Brandl CJ

(2013) The C-terminal residues of Saccharomyces cerevisiae Mec1 are required for its Localization, Stability and Function. G3: Genes, Genomes, Genetics 3:1661-1674. 2. Da-Silva LF and Duncker BP (2007) ORC function in late G1: maintaining the license for DNA replication. Cell Cycle 6:128-130.

3. Semple JW, Da-Silva LF, Jervis EJ, Ah-Kee J, Al-Attar H, Kummer L, Heikkila JJ, Pasero P, Duncker BP (2006) An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes. EMBO J 25:5150-5158. 4. Hutt DM, Da-Silva LF, Chang LH, Prosser DC, Ngsee JK (2000) PRA1 inhibits the extraction of membrane-bound rab GTPase by GDI1. J Biol Chem 275:185**-*****

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