Talent (Sergey) I. Shevchenko
Address: ***, ******** ******* **, ********, VA 20176-6678
Cell: 434-***-****
E-mail: acte3u@r.postjobfree.com
Citizenship: USA, Australia
Security Clearance: Confidential
EDUCATION
1992 post-graduate course in Biochemistry, Postgraduate Course, Moscow State University, Russia
1991 graduate course in Computer Sciences, Graduate Course, Kiev State University, Ukraine
1982 graduate course in Biology and Biochemistry, Graduate Course, Kiev State University, Ukraine
PROFESSIONAL EXPERIENCE
2012-2015 Clinical Data Manager II, Harris Inc, Alexandria, VA
2007-2012 Laboratory and Research Specialist II, UVa, Charlottesville, VA
2007-2007 Research Associate (temporal position), URI, Kingston, RI
2004-2006 Research Associate, UVa, Charlottesville, VA
1999-2004 Research Associate, UT, Memphis, TN
1996-1999 Research Assistant, Honorary Visiting Fellow, UNSW, Sydney, Australia
1994-1995 Research Fellow, BGU, Beer-Sheba, Israel
1992-1996 Scientific Engineer I, Institute for Experimental Oncology, Kiev, Ukraine
1990-1992 Scientific Researcher, Kiev State University, Kiev, Ukraine
1986-1989 PostGraduate Fellow, Moscow State University, Moscow, Russia
1982-1986 Scientific Engineer, Plant Physiology Institute, Kiev, Ukraine
KEY PROFESSIONAL SKILL
-natural and recombinant protein purification and analysis (calpain 3, RyR I, Kir 6.2, Ca+2-ATPases, H+-ATPase)
-separation methods (HPLC, PAGE, LC, GC, ultracentrifugation, ultrafiltration, other)
-molecular biology techniques, PCR
-cell culture maintenance, support, analysis (COS-1, COS-6, CHO, HEK293, MC38 cells)
-pharmacokinetic study
-animal handling, injections, necropsy
-animal survival surgery
-nanoemulsions, liposomes, microbubbles preparation for oncotherapy, drug/gene delivery, diagnostics
-immunoanalysis (histoimmunochemistry, western, other)
-patch-clamp (in brain slices, dissociated single cells, nucleated patch)
-clinical data acquisition and analysis, databases management
OTHER TRAINING
-Microsoft Office Specialist Certificate Excel 2013
-Transgenic Methods and Applications Course Certificate, University of Virginia, 2011
-Rodent Biomethodology, University of Virginia, 2008
-Proteomics: Principles and Methods Course Certificate, NIH, 2006
-Java 5.0 programming, University of Virginia, 2005-2006
-Recombinant DNA Techniques Certificate, UNSW, Australia, 1998
-Protein Engineering and Engineering Enzymology
High Qualification Course Certificate, Moscow State University, Russia, 1990
SCIENTIFIC PAPERS
1. M. Böhmer, C. Chlon, M. Dingemans, B. Raju, C. Chin, T. Shevchenko, A. Klibanov, Quantification of albumin extravasation in mouse tumor and muscle tissue with lipid and polymer microbubbles using focused ultrasound, in preparation
2. J. L., Tlaxca, J. J., Rychak, P. B., Ernst, K. R., Prasad, T. I., Shevchenko, T., Pizzaro, J. Rivera-Nieves, A. L., Klibanov, M. B., Lawrence, Ultrasound-based molecular imaging and specific gene delivery to mesenteric vasculature by endothelial adhesion molecule targeted microbubbles in a mouse model of Chron’s Disease (2013) J of Control Release, 165, 3, P216
3. S., Hernot, S., Unnikrishnan, T., Shevchenko, B., Cosyns, A., Broisat, J., Toczek, V., Caeliers, S., Muyldermans, T., Lahoutte, A. L., Klibanov, N., Devoogdt, Nanobody-Targeted Microbubbles as New Molecular Tracer (2012) J of Control Release, 158, 2, P346
4. A. L. Klibanov, T. I. Shevchenko, B. I. Raju, R. Seip and C. T. Chin, Ultrasound-triggered release of materials entrapped in microbubble-liposome constructs: A tool for targeted drug delivery (2010) J of Control Release, 148, 1, P13
5. M. R. Böhmer, C. H. T. Chlon, B. I. Raju, C. T. Chin, T. Shevchenko, A. Klibanov, Focused Ultrasound and Microbubbles for Enhancing Extravasation (2010) J of Control Release, 148, 1, P18
6. C.T. Chin, T. Shevchenko, B. I. Raju, A. L. Klibanov, Control and reversal of tumor growth by ultrasound activated microbubbles (2009) IEEE Int. Ultrasonics Symp., P77
7. T. Shevchenko, T. Diman, Social aspects of coffee consumption in Eurasia (2008) Products and Ingredients, 5, P22
8. T. Diman, T. Shevchenko, Trend in dietology: MyPyramid (2008) Mjasnoe delo, 1, P13
9. T. Diman, T. Shevchenko, Nanotechnology in food industry: nanofood (2007) Mjasnoe delo, 12, P50
10. T. Shevchenko, R. Teruyama, W. Armstrong, Kv3-like potassium channels in magnocellular neurosecretory neurons and their role in spike repolarization (2004) J. Neurophysiol. 92 (5) P3043
11. P. Roper, J. Callaway, T. Shevchenko, R. Teruyama, W. Armstrong, AHP's, HAP's and DAP's: How potassium currents regulate the excitability of rat supraoptic neurones, (2003) J Comput Neurosci., 15, P367
12. S. Shevchenko, Wei Feng, M. Varsanyi, V. Shoshan-Barmatz, Identification, characterization and partial purification of a thiol-protease which cleaves specifically the skeletal muscle ryanodine receptor/ Ca++ channel (1998) J of Membr Biol., 161(1), P33
13. S. Shevchenko, Skeletal muscle Ca++ release system functioning without calsequestrin (1998) Kandidat biologicheskih nauk thesis
14. G. Ostrovskaya, S. Shevchenko, B. Mogilevich, R. Rybalchenko, V. Rybalchenko, The surface activity of vasopressin and its interaction with artificial and biological membranes (1992) Biull of Eksp Biol Med. 114(11), P470
15. N. Stecenko, S. Shevchenko, Biological aspects on the loss of the life-giving ability for ONOCLEACEAE spores (1989) Biulleten' Glavnogo botanicheskogo sada, N.151, P58
16. S. Shevchenko, T. Chernova, E. Menshikova, V. Ritov, The blocking effect of aliphatic hydrocarbons on Ca++ leakage channels formed by sarcoplasmic reticulum Ca++- ATPase aggregates (1989) Biokhimiya 54(9), P1526
17. Karasev, G.S., Shevchenko, S.I. Structural and functional changes in wheat microsomal membranes at low temperatures (1986) 2nd All-Union Young Scientists Conference on Plant Cell Physiology, Moscow, Plant Physiology Institute, AS USSR, P89
18. Kolosha, O.I., Dubur, G.Y., Ryaboklyach, V.A., Karasev, G.S., Ozols, Y.Y., Tirzit, G.D., Shevchenko, S.I. Lipid peroxide oxidation products changes in the membranes of the wheat seedlings at low temperatures(1983) 1st All-Union Young Scientists Conference on Plant Cell Physiology, Moscow, Plant Institute, AS USSR, P48
PRESENTATIONS AT SCIENTIFIC MEETINGS
1. T. Shevchenko, A Segment of Ecotrophology Urban Agriculture Escalates (2013) Progress, problems and perspectives of ecologically safe production, BNAU, Bila Tserkva, Ukraine
2. T. Shevchenko, Revising Natural Products Efficacy For Ophthalmic Pharmacology (2013) Progress, problems and perspectives of ecologically safe production, BNAU, Bila Tserkva, Ukraine
3. S. Unnikrishnan, Z. Du, S. Hernot, J. Tlaxca, T. Shevchenko, J. Backer, M. Backer, D. Smith, J. Rychak, J. Gigliotti, K. Kalantarinia, D. Rosin, M. Okusa, M. Lawrence, J. Hossack, A. Klibanov. Targeted microbubbles as ultrasound contrast for molecular imaging applications: toward quantitative evaluation of molecular markers of disease (2012) AIUM, Phoenix, AZ
4. A. Klibanov, T. Shevchenko, W. Shi, Z. Du, R. Seip, E.Leyvi, S. Sethuraman, B. Raju, Ultrasound-triggered Release of Doxorubicin from Liposome-Microbubble Complex Slows Down Tumor Growth in a Murine Model (2011) RSNA, Chicago, IL
5. W.T. Shi, T. Shevchenko, B.I. Raju, S. Sethuraman, Z. Du, R. Seip, E.Leyvi, A.L. Klibanov, Ultrasound Therapy with Liposome-microbubble Complex in a Murine Tumor Model (2011) IEEE International Ultrasonics Symposium, Orlando, FL
6. A.L., Klibanov, T.I., Shevchenko, M.F., Campa, Z., Du, Activation of Intravascular Doxorubicin-Liposome-Microbubble Complexes by Focused Ultrasound Inhibits Tumor Growth in Mice. (2011) WMIC, San-Diego, CA
7. J. Tlaxca, J., Rychak, T., Shevchenko, A., Klibanov, M., Lawrence, Coupling Molecular Imaging of Inflammatory Bowel Disease with Targeted Gene Delivery by Sonoporation (2011) WMIC, San-Diego, CA
8. S. Hernot, S. Unnikrishnan, T. Shevchenko, B. Cosyns, A. Broisat, J. Toczek, V. Caveliers, S. Muyldermans, T. Lahoutte, A. L. Klibanov, N. Devoogdt Nanobody-Targeted Microbubbles as New Molecular Tracer, (2011) WMIC, San-Diego, CA
9. J.L. Tlaxca, J.J. Rychak, T.I., Shevchenko, A.L., Klibanov, M.B., Lawrence, Targeted Gene Delivery by Sonoporation in Crohn’s Disease (2011) ISTU, New-York, NY.
10. A.L. Klibanov, T.I. Shevchenko, Z. Du, B. Kundu, B.I. Raju, R. Seip, C.T. Chin. Ultrasound-triggered microbubbles destruction to modulate tumor vasculature blood flow (2010) FUSF, Washington DC
11. A. Klibanov, T. Shevchenko, B. Raju, R. Seip, C. Chin. Liposome-microbubble complexes: ultrasound-triggered drug release (2010) International Liposome Research Days and Lipids, Liposomes and Membrane Biophysics, Vancouver, Canada
12. A. Klibanov, T. Shevchenko, Z. Du, B. Kundu, R. Seip, B. Raju, C. Chin. Induction of Leukocyte Accumulation by Image-Guided Ultrasound Therapy: Effect Monitoring by Ultrasound Molecular Imaging (2010) WMIC, Kyoto, Japan
13. A. Klibanov, T. Shevchenko, Z. Du, R. Seip, B. Raju, C. Chin. Leukocyte Accumulation Caused by the Ultrasound-Mediated Microbubble Destruction in the Tumor Vasculature: Monitoring by Ultrasound Contrast Molecular Imaging (2010) RSNA Meeting, Chicago, IL
14. A.Klibanov, T. Shevchenko, Z. Du, B. Kundu, R. Seip, B. Raju, C. T. Chin "Drugless" tumor therapy by image-guided insonation of microbubbles in the tumor vasculature: possible mechanisms of action (2010) NIH-NCIGT 3rd Annual Image-Guided Therapy Workshop, Arlington, VA
15. A.L.Klibanov, T.I.Shevchenko, B.Kundu, Z.Du, M.Bohmer, B.I.Raju, R.Seip, C.T.Chin. Tumor therapy by microbubbles and ultrasound: mechanism of tumor growth control (2010) The 15th symposium on Ultrasound Contrast Imaging, Rotterdam, Netherlands
16. A. Klibanov, T. Shevchenko, B. Kundu, Z. Du, M. Bohmer, B. Raju, R. Seip, C. Chin. Tumor therapy by microbubbles and ultrasound: insonation of microbubbles in the tumor vasculature inhibits tumor growth (2010) IEEE International Symposium on Biomedical Imaging, Rotterdam, Netherlands
17. M. Böhmer, C. Chlon, B. Raju, C. Chin, T. Shevchenko and A. Klibanov, Focused Ultrasound and microbubbles for enhanced extravasation (2010) 11th European Symposium on Controlled Drug Delivery Proceedings, Egmond-aan-zee, Netherlands
18. A. Klibanov, T. Shevchenko, B. Raju, R. Seip, C.Chin, Ultrasound-triggered release of materials entrapped in microbubble-liposome constructs: a tool for targeted drug delivery (2010) 11th European Symposium on Controlled Drug Delivery Proceedings, Netherlands
19. A.L. Klibanov, T. Shevchenko, B. Kundu, R. Seip, B.I. Raju, C.T. Chin. Ultrasound contrast media as a therapy for solid tumor: reduction of tumor size as the result of ultrasound treatment of microbubbles in the tumor vasculature (2009) Contrast Media Research symposium, Copenhagen, Denmark
20. Chin C.T., Shevchenko T., Balasundar R., Klibanov A., Arrest of tumor growth and reduction of tumor size in vivo by combination of ultrasound and intravascular microbubbles (2009) IEEE Ultrasonics Symposium Proceedings, Roma, Italy
21. A.L. Klibanov, T. Shevchenko, S. Unnikrishnan, J. Rychak, C. Anderson, L. Phillips, A. Patil, C. Chin, B. Raju, R. Seip, C. Hall, A. Ham, M. Lawrence, J. Hossack, K. Ley. Targeted Ultrasound Agents. Imaging 2020 (2009) Jackson Hole, Wyoming
22. A.L. Klibanov, T. Shevchenko, M. Celebi, C. Hall, and C. Chin, Reduction of blood flow in the tumor vasculature after microbubble destruction by ultrasound (2009) RSNA Meeting, Chicago, IL
23. A. Klibanov, T. Shevchenko, B. Kundu, M. Bohmer, R. Seip, B. Raju, C. Chin, Destruction of microbubbles in the tumor vasculature by image-guided focused ultrasound inhibits tumor growth. (2009) World Molecular Imaging Congress, Montreal, Canada
24. A.L.Klibanov, J.Rychak, T.Shevchenko, A. van Wamel, M. Celebi, E. Ferrante, C.Chin, B.Verhaagen, C.Anderson, J.Baker, M.Baker, S.Unnikrishnam, J.Hossak, K.Ley, Lipid-based microbubbles and bubble-liposome complexes as molecular imaging agents: approaches to targeted imaging and therapy (2009) Meeting on Nanotechnology Liposomes and Health, Bahia, Brazil
25. A.L. Klibanov, J.J. Rychak, A.J.E.T. van Wamel, M. Celebi, C. Anderson, T.I. Shevchenko, J. Backer, J. Hossack, K. Ley, Targeted US bubbles (2009) European Congress of Radiology, Vienna, Austria
26. C.T. Chin, T.I. Shevchenko, B. Verhaagen, A.L. Klibanov Liposome-microbubble pendants allow ultrasound-triggered release of liposome contents (2008) MRgFUS Symposium, Washington DC
27. T.I. Shevchenko, C.T. Chin, A.L. Klibanov, Liquid perfluorocarbon droplets: ultrasound-activated enhancers of ultrasound imaging and therapy.(Phospholipid emulsion loaded with fluorocarbon as potential stable contrasting agent for ultrasound imaging) (2008) MRgFUS Symposium, Washington DC (oral presentation)
28. A. L. Klibanov, J. Rychak, T. Shevchenko, A. van Wamel, N. de Jong, M. Celebi, Y. Cho, W. Yang, J. Backer, M. Backer, J. Hossack, E. Ferrante, E. Galkina, K. Ley, Chien T. Chin, C. Hall, Lipid monolayer-coated microbubbles and liposome constructs – ultrasound contrast agents for molecular imaging and drug delivery (2008) The 11th Liposome Research Days Conference, Yokohama, Japan
29. A.L. Klibanov, C.T. Chin, W.T. Shi, M. Bohmer, C. Hall, J.J. Rychak, Y.K. Cho, A. van Wamel, N. de Jong, M. Celebi, T. Shevchenko, J. Backer, M. Backer, J. Hossack, K. Ley Microbubbles as a tool for targeted ultrasound imaging and drug delivery (2008) NIH workshop Clinical Image-Guided Therapy: Opportunities and Needs, Rockville, Maryland
30. 2. Roper, P., Shevchenko, T.I., Armstrong, W.E., Modelling the control of neuro-secretion by potassium current interactions in rat SON magnocellular neurones International (2002) Congress of Neuroendocrinology, Bristol, U.K
31. Fan, Z., Wang, C., Shevchenko, T.I., Cui, Y., Direct interaction between phosphatidylinositols and Kir6.2 activates the channel (2001) Biophys J. (Annual Meeting Abstracts) 79:1(part 2 of 2 ), P629A
32. Shevchenko, T.I., Armstrong, W.E., High threshold, TEA-sensitive potassium currents and their role in spike repolarization in supraoptic neurons (2001) SFN 31st Annual Meeting, San Diego
33. Fryer, W.M., Posterino, C.G., Shevchenko S. Abolition of E-C coupling by high intracellular Ca++ does not involve connectin/beta-connectin proteolysis (1998) APPS/FAOPS/FAONS/PSNZ Meeting, Brisbane
34. Ritov, V.B., Shevchenko, S.I., Rybalchenko,V.K. Alamethicin-induced complete calsequestrin release from SR vesicles ( 1992 ) 4th Conference on Biochemistry, Kiev, Institute of Biochemistry, AS Ukraine, P198
35. Rybalchenko, V.K., Ostrovskaya, G.V., Mogilevich, B.R., Rybalchenko, R.V., Shamonina, A.M., Shevchenko, S.I. "Lipid way"- interaction for peptide and cell membranes ( 1992 ) Conference on the 150-anniversary of the Kiev University Physiology Department, Kiev, Kiev University, P26
36. Rybalchenko,R.V.,Ostrovskaya,G.V., Mogilevich,B.R., Gorbacheva, R.V., Shevchenko S.I. Neurohypophysal hormones: mechanisms of the effect and membrane activity (1991) International Conference "Neuropharmacology on the borderline of the centuries", Leningrad, Leningrad State University, P12
37. Shevchenko, S.I., Ritov, V.B. Alamethicin induced calsequestrin release from SR vesicles (1989) The All-Union Conference on Muscle Biochemistry,Tbilisi, Biochemistry Institute, AS Georgia, P415
38. Karasev, G.S., Shevchenko,S.I. The action of fatty acids and lipid peroxidation products on wheat vegetation at low temperatures (1984) 4th Young Biologists Conference "Biological aspects of plant and animal productivity", Riga, Biology Institute, AS Latvia, P25